BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149547.6 + phase: 0
(160 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227194 similar to UP|Q8T5S8 (Q8T5S8) Prolyl 4-hydroxylase alph... 248 9e-67
TC232158 227 2e-60
TC228929 226 4e-60
TC216902 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alph... 160 3e-40
TC219496 153 4e-38
TC219497 152 5e-38
TC227195 112 1e-25
BE822836 94 3e-20
BE823168 77 5e-15
TC229833 77 5e-15
TC222092 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, parti... 75 1e-14
TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alp... 70 4e-13
BI971299 65 1e-11
TC222832 similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, par... 64 2e-11
AW472148 58 2e-09
TC222216 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alph... 51 2e-07
TC222148 weakly similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxyla... 46 9e-06
AW278519 37 0.003
TC234894 weakly similar to UP|ELK3_HUMAN (P41970) ETS-domain pro... 34 0.034
TC225985 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer pro... 28 2.4
>TC227194 similar to UP|Q8T5S8 (Q8T5S8) Prolyl 4-hydroxylase alpha-related
protein PH4[alpha]PV (CG31015-PA), partial (4%)
Length = 965
Score = 248 bits (633), Expect = 9e-67
Identities = 118/179 (65%), Positives = 139/179 (76%), Gaps = 22/179 (12%)
Frame = +1
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILH 59
MHKSTV D ETGKS D+ RTSSGTF+ RG DKI+R+IE+RIA ++FIPVE+GE + +LH
Sbjct: 37 MHKSTVVDSETGKSKDSRVRTSSGTFLARGRDKIVRDIEKRIAHYSFIPVEHGEGLQVLH 216
Query: 60 YEVGQKYEPHPDFFTDEINTKNGGERVATMLMYL---------------------PWWNE 98
YEVGQKYEPH D+F D+ NTKNGG+R+AT+LMYL PWWNE
Sbjct: 217 YEVGQKYEPHYDYFLDDFNTKNGGQRIATVLMYLTDVEEGGETVFPAAKGNFSSVPWWNE 396
Query: 99 LSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRVGKWS 157
LS+CGKKGLSIKPK GDALLFWSMKPD TLDP S+HG CPVIKG+KWS TKWMRV +++
Sbjct: 397 LSECGKKGLSIKPKRGDALLFWSMKPDATLDPSSLHGGCPVIKGNKWSSTKWMRVSEYN 573
>TC232158
Length = 904
Score = 227 bits (579), Expect = 2e-60
Identities = 106/173 (61%), Positives = 132/173 (76%), Gaps = 22/173 (12%)
Frame = +2
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILH 59
M KSTV D+++G+SV + R S+G F++RG D+I+RNIE+RIAD TFIP+ENGE + ++H
Sbjct: 299 MQKSTVADNQSGQSVVHDVRKSTGAFLDRGQDEIVRNIEKRIADVTFIPIENGEPIYVIH 478
Query: 60 YEVGQKYEPHPDFFTDEINTKNGGERVATMLMYL---------------------PWWNE 98
YEVGQ Y+PH D+F D+ N +NGG+R+ATMLMYL PWWNE
Sbjct: 479 YEVGQYYDPHYDYFIDDFNIENGGQRIATMLMYLSNVEEGGETMFPRAKANFSSVPWWNE 658
Query: 99 LSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWM 151
LS+CGK GLSIKPKMGDALLFWSMKP+ TLD L++H ACPVIKG+KWSCTKWM
Sbjct: 659 LSNCGKMGLSIKPKMGDALLFWSMKPNATLDALTLHSACPVIKGNKWSCTKWM 817
>TC228929
Length = 979
Score = 226 bits (576), Expect = 4e-60
Identities = 103/165 (62%), Positives = 124/165 (74%), Gaps = 21/165 (12%)
Frame = +3
Query: 15 DNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVGQKYEPHPDFFT 74
++ RTSSG F+ RG DKI++NIE+RIADFTFIP ENGE + ILHYEVGQKYEPH D+F
Sbjct: 9 ESRVRTSSGMFLKRGRDKIIQNIEKRIADFTFIPEENGEGLQILHYEVGQKYEPHYDYFL 188
Query: 75 DEINTKNGGERVATMLMYL---------------------PWWNELSDCGKKGLSIKPKM 113
DE NTKNGG+R+AT+LMYL PWWN+LS C +KGLS+KPKM
Sbjct: 189 DEFNTKNGGQRIATVLMYLSDVEEGGETVFPAANANFSSVPWWNDLSQCARKGLSVKPKM 368
Query: 114 GDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRVGKWSI 158
GDALLFWSM+PD TLDP S+HG CPVIKG+KWS TKWM + ++ +
Sbjct: 369 GDALLFWSMRPDATLDPSSLHGGCPVIKGNKWSSTKWMHLREYKV 503
>TC216902 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (89%)
Length = 1381
Score = 160 bits (404), Expect = 3e-40
Identities = 76/173 (43%), Positives = 110/173 (62%), Gaps = 23/173 (13%)
Frame = +3
Query: 4 STVDDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVG 63
+ D+ +G+S + RTSSG FI++ D I+ IE +I+ +TF+P ENGE + +L YE G
Sbjct: 399 AVADNLSGESQLSDVRTSSGMFISKNKDPIVAGIEDKISSWTFLPKENGEDIQVLRYEHG 578
Query: 64 QKYEPHPDFFTDEINTKNGGERVATMLMYLP-----------------------WWNELS 100
QKY+PH D+FTD++N GG R+AT+LMYL ++LS
Sbjct: 579 QKYDPHYDYFTDKVNIARGGHRIATVLMYLTDVAKGGETVFXSAEEPPRRRGAETSSDLS 758
Query: 101 DCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRV 153
+C KKG+++KP+ GDALLF+S+ + T D S+H CPVI+G+KWS TKW+ V
Sbjct: 759 ECAKKGIAVKPRRGDALLFFSLHTNATPDTSSLHAGCPVIEGEKWSATKWIHV 917
>TC219496
Length = 910
Score = 153 bits (386), Expect = 4e-38
Identities = 83/170 (48%), Positives = 109/170 (63%), Gaps = 18/170 (10%)
Frame = +1
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDK--ILRNIEQRIADFTFIPVENGESVNI 57
+H STV D +TGK + + RTSSG F+N K +++ IE+RI+ ++ IP+ENGE + +
Sbjct: 232 LHISTVVDTKTGKGIKSDVRTSSGMFLNSKERKYPMVQAIEKRISVYSQIPIENGELMQV 411
Query: 58 LHYEVGQKYEPHPDFFTDEINTKNGGERVATMLMY-------------LPWWNELSDCGK 104
L YE Q Y+PH D+F+D N K GG+R+ATMLMY L E S GK
Sbjct: 412 LRYEKNQYYKPHHDYFSDTFNLKRGGQRIATMLMYLSDNIEGGETYFPLAGSGECSCGGK 591
Query: 105 --KGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMR 152
KGLS+KP G+A+LFWSM DG DP S+HG C VI G+KWS TKW+R
Sbjct: 592 LVKGLSVKPIKGNAVLFWSMGLDGQSDPNSVHGGCEVISGEKWSATKWLR 741
>TC219497
Length = 770
Score = 152 bits (385), Expect = 5e-38
Identities = 81/166 (48%), Positives = 106/166 (63%), Gaps = 17/166 (10%)
Frame = +3
Query: 4 STVDDETGKSVDNSARTSSGTFINRGHDK--ILRNIEQRIADFTFIPVENGESVNILHYE 61
+ VD +TGK + + RTSSG F+N K +++ IE+RI+ ++ IP+ENGE + +L YE
Sbjct: 33 NVVDTKTGKGIKSDVRTSSGMFLNPQERKYPMVQAIEKRISVYSQIPIENGELMQVLRYE 212
Query: 62 VGQKYEPHPDFFTDEINTKNGGERVATMLMY-------------LPWWNELSDCGK--KG 106
Q Y+PH D+F+D N K GG+R+ATMLMY L E S GK KG
Sbjct: 213 KNQYYKPHHDYFSDTFNLKRGGQRIATMLMYLSDNIEGGETYFPLAGSGECSCGGKLVKG 392
Query: 107 LSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMR 152
LS+KP G+A+LFWSM DG DP S+HG C VI G+KWS TKWMR
Sbjct: 393 LSVKPIKGNAVLFWSMGLDGQSDPNSVHGGCEVISGEKWSATKWMR 530
>TC227195
Length = 594
Score = 112 bits (279), Expect = 1e-25
Identities = 49/61 (80%), Positives = 54/61 (88%)
Frame = +2
Query: 96 WNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRVGK 155
WNELS+CGKKGLSIKPK GDALLFWSMKPD TLDP S+HG CPVIKG+K S TKWMRV +
Sbjct: 11 WNELSECGKKGLSIKPKRGDALLFWSMKPDATLDPSSLHGGCPVIKGNKGSSTKWMRVSE 190
Query: 156 W 156
+
Sbjct: 191 Y 193
>BE822836
Length = 610
Score = 94.0 bits (232), Expect = 3e-20
Identities = 51/122 (41%), Positives = 70/122 (56%), Gaps = 17/122 (13%)
Frame = -2
Query: 47 IPVENGESVNILHYEVGQKYEPHPDFFTDEINTKNGGERVATMLMYL-----------PW 95
IP +G NIL YEV QKY+ H D F + +R+A+ L+YL P+
Sbjct: 606 IPXTHGXIFNILKYEVXQKYDSHYDAFNPDEYXXVESQRIASFLLYLSNVEAGGETMFPY 427
Query: 96 WNELS-DCGKK-----GLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTK 149
L+ D G GL +KP+ GD LLF+S+ P+G +D S+HG+CPVIKG+KW TK
Sbjct: 426 EGGLNIDRGYDYQKCIGLKVKPRQGDGLLFYSLLPNGKIDKTSLHGSCPVIKGEKWVATK 247
Query: 150 WM 151
W+
Sbjct: 246 WI 241
>BE823168
Length = 654
Score = 76.6 bits (187), Expect = 5e-15
Identities = 30/55 (54%), Positives = 43/55 (77%)
Frame = -1
Query: 99 LSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGACPVIKGDKWSCTKWMRV 153
L +C +KG+++KP+ GDALLF+S+ P+ D LS+H CPVI+G+KWS TKW+ V
Sbjct: 546 LXECAQKGIAVKPRRGDALLFFSLYPNAIPDTLSLHAGCPVIEGEKWSATKWIHV 382
>TC229833
Length = 1265
Score = 76.6 bits (187), Expect = 5e-15
Identities = 41/136 (30%), Positives = 70/136 (51%), Gaps = 16/136 (11%)
Frame = +3
Query: 31 DKILRNIEQRIADFTFIPVENGESVNILHYEVGQKYEPHPDFFTDEINTKNGGERVATML 90
D IL IE+R++ + F+P E + + ++HY Q + D+FT++ + G +AT++
Sbjct: 444 DDILARIEERLSVWAFLPKEYSKPLQVMHYGPEQNGR-NLDYFTNKTQLELSGPLMATII 620
Query: 91 MYL----------------PWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMH 134
+YL P + S C ++P G+A+LF+S+ P + D S H
Sbjct: 621 LYLSNDVTQGGQILFPESVPGSSSWSSCSNSSNILQPVKGNAILFFSLHPSASPDKSSFH 800
Query: 135 GACPVIKGDKWSCTKW 150
CPV++GD WS K+
Sbjct: 801 ARCPVLEGDMWSAIKY 848
>TC222092 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partial (26%)
Length = 455
Score = 75.5 bits (184), Expect = 1e-14
Identities = 35/83 (42%), Positives = 51/83 (61%), Gaps = 3/83 (3%)
Frame = +2
Query: 80 KNGGERV---ATMLMYLPWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLDPLSMHGA 136
+ GGE + A + P S+C KG ++KP+ GDALLF+ + D + D S+HG+
Sbjct: 5 EKGGETIFPNAEAKLLQPKDESWSECAHKGYAVKPRKGDALLFFILHLDASTDTKSLHGS 184
Query: 137 CPVIKGDKWSCTKWMRVGKWSIP 159
CPVI+G+KWS TKW+ V + P
Sbjct: 185 CPVIEGEKWSATKWIHVSDFEKP 253
>TC227193 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (41%)
Length = 557
Score = 70.1 bits (170), Expect = 4e-13
Identities = 35/51 (68%), Positives = 43/51 (83%), Gaps = 1/51 (1%)
Frame = +1
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVE 50
MHKS+V D ETGKS D+ RTSSGTF+ RG DKI+R+IE+RIA ++FIPVE
Sbjct: 403 MHKSSVVDSETGKSKDSRVRTSSGTFLARGRDKIVRDIEKRIAHYSFIPVE 555
>BI971299
Length = 669
Score = 65.1 bits (157), Expect = 1e-11
Identities = 39/141 (27%), Positives = 65/141 (45%), Gaps = 21/141 (14%)
Frame = -1
Query: 31 DKILRNIEQRIADFTFIPVENGESVNILHYEVGQKYEPHP-----DFFTDEINTKNGGER 85
D IL IE+R++ + E + ++HY P P D+FT + + G
Sbjct: 657 DDILARIEERLSXXXXLXXEYSKPXQVMHYG------PEPNGRNLDYFTXKTQLELSGPL 496
Query: 86 VATMLMYL----------------PWWNELSDCGKKGLSIKPKMGDALLFWSMKPDGTLD 129
+AT+++YL P + S C ++P G+A+LF+S+ P + D
Sbjct: 495 MATIVLYLSNAATQGGQILFPESVPRSSSWSSCSNSSNILQPVKGNAILFFSLHPSASPD 316
Query: 130 PLSMHGACPVIKGDKWSCTKW 150
S H CPV++G+ WS K+
Sbjct: 315 KNSFHARCPVLEGNMWSAIKY 253
>TC222832 similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, partial (57%)
Length = 619
Score = 64.3 bits (155), Expect = 2e-11
Identities = 39/94 (41%), Positives = 54/94 (56%), Gaps = 4/94 (4%)
Frame = +1
Query: 4 STVDDETGKSVDNSA--RTSSGTFINRGHDKI--LRNIEQRIADFTFIPVENGESVNILH 59
ST+ G++ DN+ RTSSG F++ DK L IE++IA T IP +GE+ NIL
Sbjct: 265 STLALRQGETEDNTKGIRTSSGVFVSASEDKTRTLDVIEEKIARATMIPRSHGEAFNILR 444
Query: 60 YEVGQKYEPHPDFFTDEINTKNGGERVATMLMYL 93
YEV Q+Y H D F +R+A+ L+YL
Sbjct: 445 YEVNQRYNSHYDAFNPAEYGPQKSQRMASFLLYL 546
>AW472148
Length = 391
Score = 58.2 bits (139), Expect = 2e-09
Identities = 30/90 (33%), Positives = 52/90 (57%)
Frame = +2
Query: 4 STVDDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIPVENGESVNILHYEVG 63
+ D+ + +S + TSS FI +D I+ +IE +I+ +TF+P+EN E + IL E G
Sbjct: 107 AVADNLSSESKVSEITTSSCMFIPNNNDLIVASIEYKISSWTFLPIENEEDIQILICEHG 286
Query: 64 QKYEPHPDFFTDEINTKNGGERVATMLMYL 93
KY PH D+ ++++ R + +L+ L
Sbjct: 287 HKYHPHYDYCANKVHITRCTHRSSHILISL 376
>TC222216 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (27%)
Length = 573
Score = 51.2 bits (121), Expect = 2e-07
Identities = 25/49 (51%), Positives = 36/49 (73%), Gaps = 1/49 (2%)
Frame = +1
Query: 1 MHKSTV-DDETGKSVDNSARTSSGTFINRGHDKILRNIEQRIADFTFIP 48
+ KS V D+E+GKS+ + RTSSG F+N+ D+I+ IE RIA +TF+P
Sbjct: 427 LEKSMVADNESGKSIMSEVRTSSGMFLNKAQDEIVAGIEARIAAWTFLP 573
>TC222148 weakly similar to UP|Q75ZI4 (Q75ZI4) Prolyl 4-hydroxylase, partial
(40%)
Length = 619
Score = 45.8 bits (107), Expect = 9e-06
Identities = 28/55 (50%), Positives = 36/55 (64%), Gaps = 2/55 (3%)
Frame = +1
Query: 9 ETGKSVDNSARTSSGTFINRGHDK--ILRNIEQRIADFTFIPVENGESVNILHYE 61
ET +S ++ RTSSGTFI+ DK IL +E++IA T IP +GE NIL YE
Sbjct: 457 ETAESTKDT-RTSSGTFISASEDKSGILDLVERKIAKVTMIPRTHGEIFNILKYE 618
>AW278519
Length = 288
Score = 37.4 bits (85), Expect = 0.003
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = +3
Query: 135 GACPVIKGDKWSCTKWMR 152
G+CPVIKG+KW TKW+R
Sbjct: 6 GSCPVIKGEKWVATKWIR 59
>TC234894 weakly similar to UP|ELK3_HUMAN (P41970) ETS-domain protein Elk-3
(ETS-related protein NET) (ETS-related protein ERP) (SRF
accessory protein 2) (SAP-2), partial (5%)
Length = 408
Score = 33.9 bits (76), Expect = 0.034
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +3
Query: 50 ENGESVNILHYEVGQKYEPHPDF 72
E+GE ++++ Y VGQ YEPH D+
Sbjct: 339 EHGEPLHVIRYAVGQYYEPHVDY 407
>TC225985 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer protein-like,
partial (40%)
Length = 1027
Score = 27.7 bits (60), Expect = 2.4
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -2
Query: 78 NTKNGGERVATMLMYLPWWNELSDCGKKGLSIK 110
N G R+ +L +LP WN C KK S K
Sbjct: 135 NRPKGATRLKRVLDFLPLWNPFWSCRKKDKSKK 37
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.136 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,319,163
Number of Sequences: 63676
Number of extensions: 119125
Number of successful extensions: 531
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 520
length of query: 160
length of database: 12,639,632
effective HSP length: 90
effective length of query: 70
effective length of database: 6,908,792
effective search space: 483615440
effective search space used: 483615440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149547.6