BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149496.4 - phase: 0
(475 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222199 105 6e-23
TC216808 59 1e-19
BG508280 57 2e-08
BI317622 55 5e-08
BE611176 40 0.002
BI315644 40 0.003
TC229114 similar to UP|Q9LQQ6 (Q9LQQ6) F24B9.6, partial (42%) 32 0.81
TC205285 31 1.4
TC208178 similar to UP|Q70YZ8 (Q70YZ8) Sorting nexin 1, partial ... 30 2.4
BF324596 similar to PIR|JW0071|JW00 asparagine synthase (glutami... 30 3.1
BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sat... 30 3.1
AW234302 29 4.0
TC222083 29 4.0
TC231098 similar to UP|SR40_YEAST (P32583) Suppressor protein SR... 29 5.2
BM094247 similar to PIR|B84668|B846 ethylene-insensitive3-like1 ... 28 6.8
TC204576 similar to UP|Q7XYW2 (Q7XYW2) Seed specific protein Bn1... 28 8.9
TC204620 similar to UP|WUN1_SOLTU (P20144) Wound-induced protein... 28 8.9
TC217228 similar to UP|Q8H0P8 (Q8H0P8) RNA-binding protein, part... 28 8.9
>TC222199
Length = 691
Score = 105 bits (261), Expect = 6e-23
Identities = 70/227 (30%), Positives = 105/227 (45%), Gaps = 16/227 (7%)
Frame = +2
Query: 72 DYQLVPTLEEYSFWVGLPVSEGEPFNGLEPSPKNATIAEALHLKPSDLVHPHFTIKNNLQ 131
D+QLVPT+EE+ +G P+ E +P+ P + I + L T +N +
Sbjct: 5 DFQLVPTIEEFEEILGCPLGERKPYLSSGCLPSLSRIPTMVKDSARGLDRVKQT-RNGIA 181
Query: 132 GLTAKFLYQKASDFVKAKKTNAFESIFTLLIYGLFLFPNMDNFVDLNAIKIFLA-----K 186
GL K+L KA F + LLI+G+ LFPN+D VDL AI FLA +
Sbjct: 182 GLPRKYLEDKARGIANQGDWVPFMDVLALLIFGVVLFPNVDGLVDLAAIDAFLAYHHSKE 361
Query: 187 NPVPTLLANTYHSIHHRNIREGGLIVCCAPLLYRWYASHLTK----------SVFSKENS 236
+PV +LA+ + + + R + I+CC P L W SHL + S S
Sbjct: 362 SPVVAVLADLFDTFNRRYEKSSARIICCLPALCVWLVSHLFQQDTRRPCPLLSHRSCTEK 541
Query: 237 GKASWSERIMPLTPADIVWV-HAGTNTAGIIGSCGEFENVPLIGTCG 282
+ W + + + I W G++ SCG + N+PL+GT G
Sbjct: 542 RRIDWDQLLAGIGGRTISWFPRWKEGKEGVLFSCGRYPNIPLVGTRG 682
>TC216808
Length = 1144
Score = 58.9 bits (141), Expect(2) = 1e-19
Identities = 35/79 (44%), Positives = 44/79 (55%), Gaps = 5/79 (6%)
Frame = +3
Query: 153 AFESIFTLLIYGLFLFPNMDNFVDLNAIKIFLA-----KNPVPTLLANTYHSIHHRNIRE 207
+F I LLI+G+ LFPNM+ VDL AI FLA ++PV +LA Y + +
Sbjct: 591 SFIDILALLIFGVVLFPNMEGLVDLAAIDAFLAFHHGKESPVVAILA-MYDTFDRGCEKS 767
Query: 208 GGLIVCCAPLLYRWYASHL 226
IVCCA LY W SHL
Sbjct: 768 SARIVCCALALYVWLVSHL 824
Score = 55.8 bits (133), Expect(2) = 1e-19
Identities = 44/148 (29%), Positives = 76/148 (50%), Gaps = 8/148 (5%)
Frame = +2
Query: 3 SKTRSIKKYTF----KKPDLTRLRELASHVTNPRD--FRERHGRLLDLLKVKVEDGILET 56
S+ S+KK + K ++T L EL + + FR+ +G++ DL +V+V + +
Sbjct: 125 SQKPSLKKRFYQVKIKSLEVTSLGELGQLMGQLQR*AFRKAYGKIWDLARVEVSMEPIAS 304
Query: 57 LVQFYDPICHCFTFPDYQLVPTLEEYSFWVGLPVSEGEP--FNGLEPSPKNATIAEALHL 114
L Q+YD CFTF D+QLVPT++E G + +P F+G PS +A+ + +
Sbjct: 305 LSQYYDQPLRCFTFEDFQLVPTVKELEEIRGCQLGGRKPYLFSGFYPS--TTRVAKVVKI 478
Query: 115 KPSDLVHPHFTIKNNLQGLTAKFLYQKA 142
+L +N + G+ K L ++A
Sbjct: 479 SAQELNRVKQN-RNGVVGVPRKCLEERA 559
>BG508280
Length = 418
Score = 57.0 bits (136), Expect = 2e-08
Identities = 41/109 (37%), Positives = 55/109 (49%), Gaps = 11/109 (10%)
Frame = +1
Query: 265 IIGSCGEFENVPLIGTCGGITYNPTLAMRQYGYPMKGRP--DSLSLSNEFYLNKNDHTNL 322
++ SCG F NVPL+GT G I+YNP LA++Q GYPM G P D L+ NK + L
Sbjct: 70 VLISCGGFPNVPLMGTRGCISYNPVLAIKQLGYPM*GVPLEDELAPVISRGFNKTNVETL 249
Query: 323 RMRFVQAWHSIRKFDGIQLG---------RKQSFAHESYTQWVIDRATA 362
+ + +AW ++K D G RK AH W+ TA
Sbjct: 250 Q-KVRKAWEVLQKKDKELRGSNNGPIGGYRKWLKAHVQGLDWLPSLRTA 393
>BI317622
Length = 370
Score = 55.5 bits (132), Expect = 5e-08
Identities = 39/123 (31%), Positives = 54/123 (43%), Gaps = 16/123 (13%)
Frame = -2
Query: 172 DNFVDLNAIKIFLAKN-----PVPTLLANTYHSIHHRNIREGGLIVCCAPLLYRWYASHL 226
D VDL AI FLA + P+ +LA+ Y + + R + IVCC P LY W SHL
Sbjct: 369 DGLVDLAAIGAFLAYHDYKEIPIVAMLADLYDTFNRRCEKSSTRIVCCTPALYVWLVSHL 190
Query: 227 ----------TKSVFSKENSGKASWSERIMPLTPADIVWV-HAGTNTAGIIGSCGEFENV 275
+S S G +W + + A + W ++ SCG F NV
Sbjct: 189 FRQEVRHACPLESHCSCTEKGGENWGQLLANKEGASVNWFPRWKEGRTRVLISCGVFLNV 10
Query: 276 PLI 278
PL+
Sbjct: 9 PLM 1
>BE611176
Length = 424
Score = 40.0 bits (92), Expect = 0.002
Identities = 22/76 (28%), Positives = 39/76 (50%), Gaps = 2/76 (2%)
Frame = -2
Query: 2 DSKTRSIKKYTFKKPDLTRLRELASHV--TNPRDFRERHGRLLDLLKVKVEDGILETLVQ 59
D + + K D+T ++EL + + FR+ + ++LDL ++ + +L Q
Sbjct: 372 DQTGKRFYQVKIKGLDITSIKELGRLMGPLQMQAFRKAYEKILDLTIAEISTEAIASLTQ 193
Query: 60 FYDPICHCFTFPDYQL 75
+YD CFTF D+QL
Sbjct: 192 YYD*PLRCFTFGDFQL 145
>BI315644
Length = 421
Score = 39.7 bits (91), Expect = 0.003
Identities = 19/40 (47%), Positives = 24/40 (59%)
Frame = +3
Query: 280 TCGGITYNPTLAMRQYGYPMKGRPDSLSLSNEFYLNKNDH 319
T G I YNP LA+RQ GYPM+G P S++ +DH
Sbjct: 276 TRGCINYNPVLAIRQLGYPMRGVPSDESITPFIARGFSDH 395
>TC229114 similar to UP|Q9LQQ6 (Q9LQQ6) F24B9.6, partial (42%)
Length = 1476
Score = 31.6 bits (70), Expect = 0.81
Identities = 23/79 (29%), Positives = 42/79 (53%), Gaps = 10/79 (12%)
Frame = +2
Query: 394 ERLAEAEREIHMWKREC--QKKDKDY-ETVMGLLEQEAYD-------SRQKDVIIAKLNE 443
E+ ++ ER ++E Q K DY T+M +E+ + SR+ D IAK++E
Sbjct: 713 EKSSKHERNALRREKEILKQAKQSDYIRTLMNDMEERPEEIRDFEGASREVDRYIAKMDE 892
Query: 444 RIKEKDAALDRIPGRKKKR 462
R ++++ R+P K++R
Sbjct: 893 RARQEEELFTRVPLTKQER 949
>TC205285
Length = 1040
Score = 30.8 bits (68), Expect = 1.4
Identities = 15/57 (26%), Positives = 31/57 (54%)
Frame = +1
Query: 393 QERLAEAEREIHMWKRECQKKDKDYETVMGLLEQEAYDSRQKDVIIAKLNERIKEKD 449
++R+ +E+ CQKK+K+Y+ + ++ +R+K +I KL E + E +
Sbjct: 457 RKRIDSVNKELKPLGHTCQKKEKEYKDALEAFNEK---NREKVQLITKLMELVGESE 618
>TC208178 similar to UP|Q70YZ8 (Q70YZ8) Sorting nexin 1, partial (47%)
Length = 1011
Score = 30.0 bits (66), Expect = 2.4
Identities = 23/80 (28%), Positives = 39/80 (48%)
Frame = +2
Query: 348 AHESYTQWVIDRATAFGMPYTLPRFLSSTVPEIPLPLLPQTKEEYQERLAEAEREIHMWK 407
A +S + +RA AF L + + EI L L + + ++AEAE E K
Sbjct: 212 AVQSIKATIAERANAFRRQCELAETMK--LKEINLDKLMLIRSD---KVAEAEHEYKELK 376
Query: 408 RECQKKDKDYETVMGLLEQE 427
E ++ K +ET++ L+ +E
Sbjct: 377 AESEQATKTFETIVKLMNEE 436
>BF324596 similar to PIR|JW0071|JW00 asparagine synthase
(glutamine-hydrolysing) (EC 6.3.5.4) - soybean, partial
(13%)
Length = 250
Score = 29.6 bits (65), Expect = 3.1
Identities = 16/36 (44%), Positives = 18/36 (49%)
Frame = -1
Query: 297 YPMKGRPDSLSLSNEFYLNKNDHTNLRMRFVQAWHS 332
YP P L L N F ++ N H N FVQ WHS
Sbjct: 181 YPFLNSP--LFLFNRFIISINVHCNSDKIFVQKWHS 80
>BI470376 weakly similar to GP|14090338|dbj P0638D12.5 {Oryza sativa
(japonica cultivar-group)}, partial (5%)
Length = 429
Score = 29.6 bits (65), Expect = 3.1
Identities = 15/58 (25%), Positives = 30/58 (50%)
Frame = -1
Query: 41 LLDLLKVKVEDGILETLVQFYDPICHCFTFPDYQLVPTLEEYSFWVGLPVSEGEPFNG 98
++ L ++KV ++ ++ + P H F + TL++ S +G+PV +G P G
Sbjct: 369 IMKLAQLKVNGALVNAFIERWRPETHTFHLKYGEATITLQDVSVLLGIPV-DGRPLIG 199
>AW234302
Length = 260
Score = 29.3 bits (64), Expect = 4.0
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +1
Query: 159 TLLIYGLFLFPNMDNFVDLNAIKIFL 184
T ++ +FLF N++N VDLN I+ FL
Sbjct: 175 TKKVFHVFLFRNLENVVDLN*IRKFL 252
>TC222083
Length = 522
Score = 29.3 bits (64), Expect = 4.0
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = +3
Query: 210 LIVCCAPLLYRWYASHLTKSVFSKENSGKASWSERIMPLTPAD 252
L VC LL R + H+T+ V S+ A WS RI ++P++
Sbjct: 273 LFVC---LLVRLHLKHITRKVLSRFLVMGACWSSRIKAVSPSN 392
>TC231098 similar to UP|SR40_YEAST (P32583) Suppressor protein SRP40, partial
(5%)
Length = 1016
Score = 28.9 bits (63), Expect = 5.2
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +2
Query: 379 EIPLPLLPQTKEEYQERLAEAEREIHMWKRECQKKDKDYE 418
+I + L + YQ + E E++ W R C KKD++ E
Sbjct: 596 KIQMLQLQEQNISYQAQALELEKQRLKWLRYCSKKDRELE 715
>BM094247 similar to PIR|B84668|B846 ethylene-insensitive3-like1 (EIL1)
[imported] - Arabidopsis thaliana, partial (3%)
Length = 428
Score = 28.5 bits (62), Expect = 6.8
Identities = 18/74 (24%), Positives = 36/74 (48%), Gaps = 3/74 (4%)
Frame = +3
Query: 389 KEEYQERLAEAEREIHMWKRECQKKDKDYETVMGLLEQE---AYDSRQKDVIIAKLNERI 445
K + + +L E ++ ++ KR+C DK + +LEQE Y ++ ++ ++E I
Sbjct: 81 KRKAEIKLEELDKNMNSIKRQCVDADKSLTSKKLVLEQEEMDLYTAKNSATPLSSVDELI 260
Query: 446 KEKDAALDRIPGRK 459
+E +I K
Sbjct: 261 EEISEIQKKIKDEK 302
>TC204576 similar to UP|Q7XYW2 (Q7XYW2) Seed specific protein Bn15D17A, partial
(66%)
Length = 1320
Score = 28.1 bits (61), Expect = 8.9
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = +1
Query: 363 FGMPYTLPRFLSSTVPEIPLPLLPQTKEEYQERLAEAEREIHMWKRECQKKD 414
FG+ TLP F SS V + L + K E+Q + E+H + ++ +KK+
Sbjct: 1132 FGLLLTLPLFFSSVVNAV----LYKLKHEWQ-----VDEEVHFYPQQ*EKKE 1260
>TC204620 similar to UP|WUN1_SOLTU (P20144) Wound-induced protein 1, partial
(73%)
Length = 1276
Score = 28.1 bits (61), Expect = 8.9
Identities = 15/32 (46%), Positives = 17/32 (52%)
Frame = +2
Query: 249 TPADIVWVHAGTNTAGIIGSCGEFENVPLIGT 280
T +I WVHA T T GII E+ N L T
Sbjct: 716 TARNIAWVHACTVTDGIITQIREYFNTALTVT 811
>TC217228 similar to UP|Q8H0P8 (Q8H0P8) RNA-binding protein, partial (36%)
Length = 698
Score = 28.1 bits (61), Expect = 8.9
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +2
Query: 369 LPRFLSSTVPEIPLPLLPQTKEEYQE 394
LP SST+P+ P PLL T+E+ Q+
Sbjct: 173 LPPTRSSTLPKTPTPLLFSTEEQMQK 250
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.138 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,575,652
Number of Sequences: 63676
Number of extensions: 342994
Number of successful extensions: 1838
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1832
length of query: 475
length of database: 12,639,632
effective HSP length: 101
effective length of query: 374
effective length of database: 6,208,356
effective search space: 2321925144
effective search space used: 2321925144
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC149496.4