BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149490.11 + phase: 0
(304 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC210448 similar to UP|CHI1_TULBA (Q9SLP4) Chitinase 1 precursor... 437 e-123
NP005387 putative narbonin 128 4e-30
TC221919 weakly similar to UP|RUAP_SOYBN (P39657) RuBisCO-associ... 111 4e-25
TC230752 weakly similar to UP|RUAP_SOYBN (P39657) RuBisCO-associ... 75 2e-24
TC205783 UP|RUAP_SOYBN (P39657) RuBisCO-associated protein, comp... 103 1e-22
TC205480 weakly similar to UP|Q08883 (Q08883) Narbonin, partial ... 77 1e-14
TC221192 similar to UP|Q8WZI7 (Q8WZI7) Ferulic acid esterase A p... 72 3e-13
BM886222 weakly similar to PIR|S51591|S51 chitinase (EC 3.2.1.14... 30 1.0
BE190308 weakly similar to GP|20453112|gb| At1g08490/T27G7_14 {A... 29 2.3
AW200928 29 3.0
AW508128 28 5.1
TC221226 weakly similar to UP|Q6T282 (Q6T282) Predicted protein,... 28 5.1
CA800269 weakly similar to PIR|T04761|T047 chitinase homolog T16... 28 6.7
TC230479 similar to UP|Q947K6 (Q947K6) CDK-activating kinase, pa... 28 6.7
TC204859 similar to UP|Q9FS26 (Q9FS26) Plastidic cysteine syntha... 27 8.8
BM270261 similar to GP|19310377|gb| At2g46320/F11C10.1 {Arabidop... 27 8.8
>TC210448 similar to UP|CHI1_TULBA (Q9SLP4) Chitinase 1 precursor (Tulip
bulb chitinase-1) (TBC-1) , partial (70%)
Length = 1157
Score = 437 bits (1123), Expect = e-123
Identities = 221/310 (71%), Positives = 257/310 (82%), Gaps = 6/310 (1%)
Frame = +1
Query: 1 MELHIPKPCFILLILTTSLF--ASTILAA---NSNLFREYIGAQFKGVKFSDVPINPNVN 55
+++ +P CF++LIL S F AST + A +S+LFREYIGA F GVKFSDVPINPNVN
Sbjct: 94 IKMALPNLCFVVLILKVSFFLLASTQVRAATSDSSLFREYIGALFNGVKFSDVPINPNVN 273
Query: 56 FHFILSFAIDYDTSS-PPSPTNGNFNIFWDTQNLSPSEVSFIKNQNPNVKVALSLGGDTV 114
FHFILSFAIDYDTSS PSPTNG FNIFWD QNL+P++VS IK +NPNVKVALSLGGDTV
Sbjct: 274 FHFILSFAIDYDTSSGSPSPTNGKFNIFWDNQNLTPTQVSSIKAKNPNVKVALSLGGDTV 453
Query: 115 QGDPVNFIPSSIDSWVSNAVSSLTSIIKTYNLDGIDVDYEHFIGDPNTFAECIGRLITTL 174
F P+S+DSWVSNAVSSLT+IIK YNLDGID+DYEHF D TFA+ IG+LI TL
Sbjct: 454 SSAFAYFDPTSVDSWVSNAVSSLTNIIKEYNLDGIDIDYEHFKADSETFAQSIGKLIQTL 633
Query: 175 KNNNVISFASIAPFDDAEVQNHYLALWKSYSNIIDYVNFQFYAYDKSTTVAQFIDYYNKQ 234
K+ VI+FASIAPFD+ EVQ+HYLALWKSY +IIDYVNFQFYAYD+STTV+QFIDY+NKQ
Sbjct: 634 KSRGVITFASIAPFDNDEVQSHYLALWKSYGHIIDYVNFQFYAYDRSTTVSQFIDYFNKQ 813
Query: 235 SSNYNSEKVLVSFVSDGSTGLGPGNGFLDGCRMLKSQQKLNGIFVYSADDSKADGFRYEK 294
SSNYN KVLVSF SDGS GL P NGF C MLK+QQKL+GIFV+ ADDSKA+GF YEK
Sbjct: 814 SSNYNGGKVLVSFNSDGSRGLIPDNGFFTACSMLKTQQKLHGIFVWCADDSKANGFSYEK 993
Query: 295 EAQEVLASPN 304
++Q++L + N
Sbjct: 994 QSQDLLVTAN 1023
>NP005387 putative narbonin
Length = 873
Score = 128 bits (321), Expect = 4e-30
Identities = 98/293 (33%), Positives = 143/293 (48%), Gaps = 22/293 (7%)
Frame = +1
Query: 31 LFREYIGAQFKGVKFSDVPIN----PNVNFHFILSFAIDYDTSSPPSPTNGNFNIFWDTQ 86
+FREYIG + D P N+ FHFIL FA + S S GNF WD++
Sbjct: 13 IFREYIGVKPNSETLHDFPHEIIDTENLEFHFILGFATESYYESGKS--TGNFEESWDSE 186
Query: 87 NLSPSEVSFIKNQNPNVKVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSIIKTYN- 145
+ V +K + P VKV +S+GG VQ F P+ + WV NA SL I + Y+
Sbjct: 187 SFGLENVKKLKERYPEVKVVISIGGRGVQ---TPFHPAEENVWVENAQESLKQIFQKYSN 357
Query: 146 -----LDGIDVDYEHFIGDPNTFAECIGRLITTLKNNN--VISFASIAPFDDAEVQNHYL 198
+DGID++YEH D FA +G LIT LK ++ I+ SIAP + +HY
Sbjct: 358 ESGSMIDGIDINYEHISSD-EPFARLVGLLITELKKDDDLNINVVSIAPSETN--SSHYQ 528
Query: 199 ALWKSYSNIIDYVNFQFYAYDKST-TVAQFIDYYNKQSSNYNSEKVLVSFVSDGSTGLG- 256
L+ + + I++V++QF K F++ + +Y+ KVL F +D
Sbjct: 529 KLYNAKKDYINWVDYQFSNQHKPVHKDDHFVEIFKTVEKDYHLRKVLPGFSTDPDDAKHN 708
Query: 257 --PGNGFLDGCRMLKSQQKLNGIFVYSADDS---KADG---FRYEKEAQEVLA 301
+ F+ GC LK L G+F ++A DS + DG F E Q++LA
Sbjct: 709 KITRDVFIGGCTRLKQTSSLPGVFFWNAHDSVTPRLDGDKPFIVELTLQQLLA 867
>TC221919 weakly similar to UP|RUAP_SOYBN (P39657) RuBisCO-associated
protein, partial (31%)
Length = 688
Score = 111 bits (278), Expect = 4e-25
Identities = 71/189 (37%), Positives = 105/189 (54%), Gaps = 1/189 (0%)
Frame = +2
Query: 25 LAANSNLFREYIGAQFKGVKFSDVPINPNVNFHFILSFAIDYDTSSPPSPTNGNFNIFWD 84
+A + +FREY K +D+P + + L+FA DYD T G F +WD
Sbjct: 95 MAGSKIVFREYTSDD-SFTKLTDIPKDVS-EIQVALTFARDYDGVGS---TQGKFIPYWD 259
Query: 85 TQNLSPSEVSFIKNQNPNVKVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSIIKTY 144
T ++P ++ K++ P+ KV +S+G + + P + +W++ A SLT IIK Y
Sbjct: 260 TAIVNPDVIANFKSKYPSAKVLVSIGNNK-RHFPFKIDEDRVTAWINAATESLTRIIKEY 436
Query: 145 NLDGIDVDYEHF-IGDPNTFAECIGRLITTLKNNNVISFASIAPFDDAEVQNHYLALWKS 203
NLDGIDV YE + +P F CIG LI TLK N VI+ ASI+P + N Y +L ++
Sbjct: 437 NLDGIDVYYEDIEVSNPAIFVHCIGELIKTLKLNKVITRASISPNIEIN-DNFYRSLHQN 613
Query: 204 YSNIIDYVN 212
+ + IDYVN
Sbjct: 614 FEDHIDYVN 640
>TC230752 weakly similar to UP|RUAP_SOYBN (P39657) RuBisCO-associated
protein, partial (9%)
Length = 1028
Score = 75.5 bits (184), Expect(2) = 2e-24
Identities = 52/139 (37%), Positives = 73/139 (52%), Gaps = 8/139 (5%)
Frame = +3
Query: 30 NLFREYIGAQFKGVKFSDVPINPNV--NFHFILSFAIDYDTSSPPSPTNGNFNIFWDTQN 87
++FR+Y FS V ++ + L+FA DYD +PTNG F WD
Sbjct: 39 SIFRQYTFDD----SFSQVFVSAKFLKEYQIALTFASDYDDEG--APTNGVFRPTWDLSK 200
Query: 88 LSPSEVSFIKNQNPNV--KVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSIIKT-- 143
++P ++ K++NPNV KV +S+G +G F P + +W+ NA SLT +IK
Sbjct: 201 VTPESIARFKDKNPNVDIKVFISIGN---RGTQHPFKPLNNKTWIDNATESLTHLIKNED 371
Query: 144 YNL--DGIDVDYEHFIGDP 160
YNL DGIDV YEH P
Sbjct: 372 YNLHVDGIDVLYEHIDASP 428
Score = 54.7 bits (130), Expect(2) = 2e-24
Identities = 44/142 (30%), Positives = 71/142 (49%), Gaps = 11/142 (7%)
Frame = +1
Query: 160 PNTFAECIGRLITTLKNNNVISFASIAPFDDAEVQNHYLALWKSYSNIIDYVNFQFYA-- 217
P F EC+G+LI LK V+S ASI+P A + +Y L+ + S +D+V++QF +
Sbjct: 427 PGDFIECVGQLIRNLKEKGVVSEASISP-SFALNEEYYPLLYSAVSFFVDWVDYQFQSEL 603
Query: 218 ---YDKSTTVAQFIDYYNKQSSNYNSEKVLVSFVSDGS--TGLGPGNGFLDGCRMLKSQQ 272
+D +T V + YN+ + Y K+ + ++ L P FL G +LK ++
Sbjct: 604 KPVFDPTTLVKR----YNELTKLYPRRKLFAGYSAENEDWATLSPIVFFLGGMDILK-KR 768
Query: 273 KLNGIFV----YSADDSKADGF 290
K G+ + Y AD D F
Sbjct: 769 KAPGVSIHYHNYYADAPNKD*F 834
>TC205783 UP|RUAP_SOYBN (P39657) RuBisCO-associated protein, complete
Length = 1353
Score = 103 bits (257), Expect = 1e-22
Identities = 70/194 (36%), Positives = 96/194 (49%), Gaps = 5/194 (2%)
Frame = +2
Query: 25 LAANSNLFREYIGAQFKGVKFSDVPINPNVN-FHFILSFAIDYDTSSPPSPTNGNFNIFW 83
++ +FRE+ F + I N+ F LS A DYD ++ TNG F +W
Sbjct: 86 MSTKFKVFREFTSDD----SFLNQVIPENITEFQVTLSLARDYDGNNS---TNGKFIPYW 244
Query: 84 DTQNLSPSEVS-FIKNQNPN---VKVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTS 139
DT+ ++P + F K P VKV +S+G Q S+ ++WVS A +SL S
Sbjct: 245 DTEKVTPEVIKKFKKKYEPTALRVKVLVSIGNKNKQFPFTIGSDSNSEAWVSEATASLKS 424
Query: 140 IIKTYNLDGIDVDYEHFIGDPNTFAECIGRLITTLKNNNVISFASIAPFDDAEVQNHYLA 199
IIKTYNLDGIDV YE + F +G L+ LK N +I+ AS A DA Y
Sbjct: 425 IIKTYNLDGIDVSYEDIAANEADFVNSVGGLVRNLKQNKLITVASFATSADAANNKFYNL 604
Query: 200 LWKSYSNIIDYVNF 213
L+ Y+ D V F
Sbjct: 605 LYAEYATFFDTVVF 646
>TC205480 weakly similar to UP|Q08883 (Q08883) Narbonin, partial (8%)
Length = 1157
Score = 76.6 bits (187), Expect = 1e-14
Identities = 69/226 (30%), Positives = 101/226 (44%), Gaps = 15/226 (6%)
Frame = +3
Query: 77 GNFNIFWDTQNLSPSEVSFIK----NQNPNVKVALSLGGDTVQGDPVNFIPSSIDSWVSN 132
G F WDT N++P + K N VKV +S+GGD DP D WV N
Sbjct: 216 GKFEPKWDTDNVTPQLIEQFKLNCDASNLPVKVFVSIGGD----DPFKVAAGGADDWVLN 383
Query: 133 AVSSLTSIIKTYNLDGIDVDYEHFIGDPNTFAECIGRLITTLKNNNVIS--FASIAPFDD 190
A ++L +I Y++DGIDV+Y + F CI LI L+ + AS+AP D
Sbjct: 384 ATTTLHHMINEYHIDGIDVNYTTIQCNQTLFVNCIKGLIKGLREALCTTGFVASLAP-DA 560
Query: 191 AEVQNHYLALWKSYSNI-IDYVNFQ----FYAYDKSTTVAQFIDYYNKQSSNYN--SEKV 243
A + YL L++S N I V FQ F D + V + K Y+ K+
Sbjct: 561 ATNETFYLPLYRSIDNSWIGTVVFQCFVDFEPLDPNRPVESLVRQVEKIVGEYDYYDSKI 740
Query: 244 LVSF--VSDGSTGLGPGNGFLDGCRMLKSQQKLNGIFVYSADDSKA 287
L+ + + + + P FL R++ S ++ G V D+ A
Sbjct: 741 LLGYSAIKEDWNKVSPPLFFLALNRLVHSWPRVKGASVKVISDTYA 878
>TC221192 similar to UP|Q8WZI7 (Q8WZI7) Ferulic acid esterase A precursor,
partial (6%)
Length = 706
Score = 72.0 bits (175), Expect = 3e-13
Identities = 47/144 (32%), Positives = 73/144 (50%), Gaps = 4/144 (2%)
Frame = +3
Query: 132 NAVSSLTSIIKTYNL-DGIDVDYEHFIGDPNTFAECIGRLITTLKNNNVISFASIAPFDD 190
NA SLTS+I ++ GIDV Y+H P F EC+G+LI LK N V+S ASI+P
Sbjct: 12 NATMSLTSLINNLHVVHGIDVLYDHIDASPADFTECVGQLIRNLKENGVVSQASISP-SS 188
Query: 191 AEVQNHYLALWKSYSNIIDYVNFQFYAYDKST-TVAQFIDYYNKQSSNYNSEKVLVSFVS 249
Q +Y L+ S +D+V++QF + D+ + YN+ + Y K+ + +
Sbjct: 189 YPNQEYYPLLYSSVPFFVDWVDYQFQSEDQPVLEPTTLVKRYNELTKVYPKTKLFAGYSA 368
Query: 250 DGS--TGLGPGNGFLDGCRMLKSQ 271
+ + P FL +LK +
Sbjct: 369 ENEDWATVSPFVFFLGAMDLLKKR 440
>BM886222 weakly similar to PIR|S51591|S51 chitinase (EC 3.2.1.14) / lysozyme
(EC 3.2.1.17) PZ precursor pathogenesis-related -
common, partial (32%)
Length = 425
Score = 30.4 bits (67), Expect = 1.0
Identities = 31/112 (27%), Positives = 52/112 (45%), Gaps = 6/112 (5%)
Frame = +1
Query: 50 INPNVNFHFILSFA-IDYDTSSPP--SPTNGNFNIFWDTQNLSPSEVSFIKNQNPNVKVA 106
INP+ H +FA +D +T+ S + F+ F T ++ +NP+VK
Sbjct: 64 INPSHFTHLFCAFAHLDPNTNKVTISSSDSSQFSTFTQT----------LQAKNPSVKTL 213
Query: 107 LSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSII---KTYNLDGIDVDYEH 155
LS+GG NF S + + S + S I ++ N G+D+D+E+
Sbjct: 214 LSIGGGFGPSLAANF--SRMARQANTRKSFIDSSIQQARSNNFLGLDLDWEY 363
>BE190308 weakly similar to GP|20453112|gb| At1g08490/T27G7_14 {Arabidopsis
thaliana}, partial (11%)
Length = 214
Score = 29.3 bits (64), Expect = 2.3
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -1
Query: 130 VSNAVSSLTSIIKTYNLDGIDVDYE 154
+SN + S TSI + YNL GID Y+
Sbjct: 127 MSNKIDSFTSIFREYNLFGIDSIYK 53
>AW200928
Length = 457
Score = 28.9 bits (63), Expect = 3.0
Identities = 13/21 (61%), Positives = 14/21 (65%)
Frame = +2
Query: 209 DYVNFQFYAYDKSTTVAQFID 229
DY Q YAYDKS T A+F D
Sbjct: 134 DYKLSQLYAYDKSRTPAEFAD 196
>AW508128
Length = 454
Score = 28.1 bits (61), Expect = 5.1
Identities = 13/36 (36%), Positives = 23/36 (63%), Gaps = 3/36 (8%)
Frame = -2
Query: 9 CFILLILTTSLF---ASTILAANSNLFREYIGAQFK 41
C I + +T L+ AST+LAA+SN+ ++G+ +
Sbjct: 363 CHISVYVTVCLYRI*ASTVLAASSNIHTTFVGSSIR 256
>TC221226 weakly similar to UP|Q6T282 (Q6T282) Predicted protein, partial
(26%)
Length = 849
Score = 28.1 bits (61), Expect = 5.1
Identities = 27/80 (33%), Positives = 32/80 (39%), Gaps = 2/80 (2%)
Frame = -3
Query: 19 LFASTILAANSNLFREYIGAQFKGVKFSDVPINPNVNFHFILSFAIDYDTSSPPSPTNGN 78
LF+ T L NS L +I + KFS NP IL PP T
Sbjct: 847 LFSFTFLVLNSTLSNHFINSSDTTAKFSPFQFNPL**EQQIL----------PPPQTLLP 698
Query: 79 FN--IFWDTQNLSPSEVSFI 96
FN IF LSP ++FI
Sbjct: 697 FNVVIFNAKLYLSPGNIAFI 638
>CA800269 weakly similar to PIR|T04761|T047 chitinase homolog T16H5.160 -
Arabidopsis thaliana, partial (14%)
Length = 434
Score = 27.7 bits (60), Expect = 6.7
Identities = 15/65 (23%), Positives = 33/65 (50%)
Frame = +1
Query: 90 PSEVSFIKNQNPNVKVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSIIKTYNLDGI 149
P ++ ++ + P VK LS+GG + + S+ + ++S + + Y +G+
Sbjct: 157 PKFINGLRYRYPPVKTLLSIGGGGSNSTAFSLMASNKHTR-QVFINSTIHVARQYGFNGL 333
Query: 150 DVDYE 154
D+D+E
Sbjct: 334 DLDWE 348
>TC230479 similar to UP|Q947K6 (Q947K6) CDK-activating kinase, partial (42%)
Length = 565
Score = 27.7 bits (60), Expect = 6.7
Identities = 15/57 (26%), Positives = 25/57 (43%)
Frame = -3
Query: 85 TQNLSPSEVSFIKNQNPNVKVALSLGGDTVQGDPVNFIPSSIDSWVSNAVSSLTSII 141
+ NLSP + P + + LGGD G ++DS++ + SLT +
Sbjct: 395 SSNLSPCKEFPSDPSRPGITLLDFLGGDKTVGPSFEITSETLDSFLGAGLESLTGSV 225
>TC204859 similar to UP|Q9FS26 (Q9FS26) Plastidic cysteine synthase 1,
partial (63%)
Length = 1092
Score = 27.3 bits (59), Expect = 8.8
Identities = 28/110 (25%), Positives = 44/110 (39%), Gaps = 17/110 (15%)
Frame = +3
Query: 47 DVPINPNVNFHFILSFAIDYDTSSPP--SPTNGNFNIFW---DTQNLSPSEVSFIKNQNP 101
D P NP V+ Y+T+ P T G +I T F+K QNP
Sbjct: 210 DNPSNPKVH----------YETTGPEIWEDTRGKIDILVAGIGTGGTVSGAGQFLKQQNP 359
Query: 102 NVKVA--------LSLGGDT----VQGDPVNFIPSSIDSWVSNAVSSLTS 139
++V + GG +QG F+P ++D V + V +++S
Sbjct: 360 KIQVIGVEPLESNILTGGKPGPHKIQGIGAGFVPRNLDQDVLDEVIAISS 509
>BM270261 similar to GP|19310377|gb| At2g46320/F11C10.1 {Arabidopsis
thaliana}, partial (27%)
Length = 421
Score = 27.3 bits (59), Expect = 8.8
Identities = 19/66 (28%), Positives = 28/66 (41%)
Frame = +1
Query: 20 FASTILAANSNLFREYIGAQFKGVKFSDVPINPNVNFHFILSFAIDYDTSSPPSPTNGNF 79
F S I+ ++ + + AQ GV + V N H + FAI +S PP P
Sbjct: 73 FVSAIIVNPLDVAKTRLQAQAAGVPYQGVCQLANTTLHDVRCFAI--SSSEPPRPCPSGC 246
Query: 80 NIFWDT 85
N + T
Sbjct: 247 NRYKGT 264
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.135 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,579,247
Number of Sequences: 63676
Number of extensions: 181819
Number of successful extensions: 1034
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 1022
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1024
length of query: 304
length of database: 12,639,632
effective HSP length: 97
effective length of query: 207
effective length of database: 6,463,060
effective search space: 1337853420
effective search space used: 1337853420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC149490.11