BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149471.20 + phase: 0
(227 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217018 238 2e-63
BF069025 132 2e-31
AW309473 99 2e-21
BG509573 50 8e-07
TC220021 similar to UP|Q8LPE9 (Q8LPE9) At5g26743, partial (41%) 35 0.021
TC216872 similar to UP|Q9SCQ0 (Q9SCQ0) RNA helicase-like protein... 33 0.081
TC216873 similar to UP|Q9SCQ0 (Q9SCQ0) RNA helicase-like protein... 32 0.18
TC229552 homologue to UP|Q9SGQ0 (Q9SGQ0) F3M18.4 (Transcription ... 30 1.2
TC227551 homologue to UP|O65879 (O65879) Translation initiation ... 29 1.5
TC234127 similar to UP|Q9FG54 (Q9FG54) Root cap protein 2-like p... 29 2.0
TC230017 similar to UP|Q39941 (Q39941) HAHB-1, partial (20%) 29 2.0
TC229638 similar to UP|Q9EMG2 (Q9EMG2) AMV244, partial (11%) 28 2.6
CO984131 28 2.6
TC226496 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90 (AT5... 28 3.4
TC206686 similar to UP|Q9SCP7 (Q9SCP7) Caffeic acid O-methyltran... 28 3.4
AW706707 28 3.4
TC213538 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase com... 28 4.4
TC221372 homologue to UP|Q6L3T9 (Q6L3T9) NAM (No apical meristem... 27 5.8
TC224933 UP|Q6K853 (Q6K853) 40S ribosomal protein S30-like, comp... 27 5.8
BE346992 homologue to GP|22000958|gb| DNA polymerase processivit... 27 5.8
>TC217018
Length = 1011
Score = 238 bits (606), Expect = 2e-63
Identities = 130/204 (63%), Positives = 159/204 (77%), Gaps = 2/204 (0%)
Frame = +2
Query: 3 KRKGRENNDDRRKKKKKKT--ETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILE 60
KRK +E++D R KKKKK+ + ++QLRFF +SA ++LSS+ELESLK + ILE
Sbjct: 86 KRKRKESSDVRSKKKKKQRSEDEEATKQLRFF----ESAMGIELSSLELESLKDNKCILE 253
Query: 61 LSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIVSPSALRS 120
+S + +S DV +LG I+ AFG W++ LCE + VEGK+ GSP+VLI++ SALR
Sbjct: 254 VSEAADS-----DVTVLGKTIRAAFGASWKEALCEGKPVEGKVIAGSPAVLIITSSALRC 418
Query: 121 IHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSR 180
I LL+GFR MT+QC A KLFSKH+KL+EQISLLKNRVNIASGTPSRIKKLID EAL LSR
Sbjct: 419 IDLLRGFRSMTEQCHAAKLFSKHMKLEEQISLLKNRVNIASGTPSRIKKLIDAEALDLSR 598
Query: 181 LQVLVLDMHPDVKGYSLFTLPQVR 204
LQVLVLD+HPDVKGYSL TLPQVR
Sbjct: 599 LQVLVLDLHPDVKGYSLLTLPQVR 670
>BF069025
Length = 520
Score = 132 bits (331), Expect = 2e-31
Identities = 78/162 (48%), Positives = 104/162 (64%)
Frame = +2
Query: 43 QLSSIELESLKADSSILELSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGK 102
+LSS++ ES K + I+E+S + +S ++ L I+ AFG W++ C+ + VEGK
Sbjct: 2 RLSSMQSESTKDNMCIMEVS*AADS-----NLYRLHKTIRAAFGASWKEAFCKWKPVEGK 166
Query: 103 IPPGSPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASG 162
+ GSP +LI+S SA I LL G MT+QC A K SKH+ ++EQIS+L NRVNI S
Sbjct: 167 VIAGSPVLLIISSSA*TCIDLLTG*HSMTEQCHAGKCLSKHMNMEEQISMLNNRVNIDSR 346
Query: 163 TPSRIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLFTLPQVR 204
S IKKLID +AL LSR Q LV D+H DV+GY L QVR
Sbjct: 347 AQSMIKKLIDADALDLSRSQGLVPDLHADVEGYCNVNLTQVR 472
>AW309473
Length = 376
Score = 99.0 bits (245), Expect = 2e-21
Identities = 58/99 (58%), Positives = 68/99 (68%)
Frame = -1
Query: 106 GSPSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPS 165
GS VLI++ SA R I L+GF MT+ C VKLF +++K +E ISLL N VNIASG S
Sbjct: 373 GSSEVLIITXSASRCIDFLRGFCSMTE*CYXVKLFLQYMKFEE*ISLLNNGVNIASGILS 194
Query: 166 RIKKLIDVEALGLSRLQVLVLDMHPDVKGYSLFTLPQVR 204
RIKK ID E L RL VLVLD++ DVKGYSL T VR
Sbjct: 193 RIKKXIDAEVLDFLRL*VLVLDLYLDVKGYSLLTFL*VR 77
>BG509573
Length = 237
Score = 50.1 bits (118), Expect = 8e-07
Identities = 33/85 (38%), Positives = 48/85 (55%)
Frame = +3
Query: 13 RRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKADSSILELSNSQNSRDTDL 72
++KKK++ + ++Q RFF +SA +SS+ELESLK + ILE S + S
Sbjct: 6 KKKKKQRSEDEEATKQXRFF----ESAMGGGVSSLELESLKDNKCILEASXAAXS----- 158
Query: 73 DVKLLGGDIKGAFGNCWRQVLCESE 97
DV LG I+ AFG + LCE +
Sbjct: 159DVTGLGKTIRAAFGASGXEALCEGK 233
>TC220021 similar to UP|Q8LPE9 (Q8LPE9) At5g26743, partial (41%)
Length = 935
Score = 35.4 bits (80), Expect = 0.021
Identities = 25/80 (31%), Positives = 40/80 (49%)
Frame = +1
Query: 108 PSVLIVSPSALRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRI 167
P L+++P+ + + K + S V ++ + Q S L V++ GTP RI
Sbjct: 40 PRFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGG-VSYVTQQSALSRGVDVVVGTPGRI 216
Query: 168 KKLIDVEALGLSRLQVLVLD 187
LI+ +L LS +Q LVLD
Sbjct: 217 IDLINGNSLKLSEVQYLVLD 276
>TC216872 similar to UP|Q9SCQ0 (Q9SCQ0) RNA helicase-like protein
(At3g53110), partial (64%)
Length = 1702
Score = 33.5 bits (75), Expect = 0.081
Identities = 15/29 (51%), Positives = 19/29 (64%)
Frame = +1
Query: 159 IASGTPSRIKKLIDVEALGLSRLQVLVLD 187
+ GTP IKK I + LG SRL++LV D
Sbjct: 703 VVIGTPGTIKKFISFKKLGTSRLKILVFD 789
>TC216873 similar to UP|Q9SCQ0 (Q9SCQ0) RNA helicase-like protein
(At3g53110), partial (67%)
Length = 1840
Score = 32.3 bits (72), Expect = 0.18
Identities = 22/86 (25%), Positives = 40/86 (45%), Gaps = 5/86 (5%)
Frame = +2
Query: 107 SPSVLIVSPSALRSIHLLKGFRFMTKQCSA-----VKLFSKHIKLQEQISLLKNRVNIAS 161
+P L + P+ +I ++ R M K V L + + ++ ++ +
Sbjct: 542 APQALCICPTRELAIQNIEVLRRMGKYTGIASECLVPLDRDAVHVSKRAPIM---AQVVI 712
Query: 162 GTPSRIKKLIDVEALGLSRLQVLVLD 187
GTP +KK I + LG +RL++LV D
Sbjct: 713 GTPGTVKKFISFKKLGTTRLRILVFD 790
>TC229552 homologue to UP|Q9SGQ0 (Q9SGQ0) F3M18.4 (Transcription factor
AtVOZ1), partial (27%)
Length = 1520
Score = 29.6 bits (65), Expect = 1.2
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 126 GFRFMTKQCSAVKL-FSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVE 174
GF F QCS L FS + K + +L + VN + IKKL+D E
Sbjct: 630 GFLFCPSQCSFFSLLFSSYKKSRSFFALSRYFVNSSFSLECSIKKLLDAE 779
>TC227551 homologue to UP|O65879 (O65879) Translation initiation factor,
complete
Length = 1632
Score = 29.3 bits (64), Expect = 1.5
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +2
Query: 148 EQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQVLVLD 187
E I L+ V++ SGTP R+ +I L +++LVLD
Sbjct: 530 EDIRKLEYGVHVVSGTPGRVCDMIKRRTLRTRAIKMLVLD 649
>TC234127 similar to UP|Q9FG54 (Q9FG54) Root cap protein 2-like protein,
partial (23%)
Length = 573
Score = 28.9 bits (63), Expect = 2.0
Identities = 14/54 (25%), Positives = 27/54 (49%)
Frame = -2
Query: 118 LRSIHLLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLI 171
+ S+ +GFRF T + + SKH Q ++ ++KN + P+ + L+
Sbjct: 446 IESLFPKRGFRFFTSTTHWI-IVSKHTPTQHKLRIIKNSFHFLQTIPNPFENLV 288
>TC230017 similar to UP|Q39941 (Q39941) HAHB-1, partial (20%)
Length = 633
Score = 28.9 bits (63), Expect = 2.0
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 123 LLKGFRFMTKQCSAVK-----LFSKHIKLQEQISLLKNRVNIASGTPSRIKKLID 172
L K + + KQC AVK L S++ KL ++ LK R +GT +KK D
Sbjct: 74 LEKEYEVLKKQCEAVKADDDSLKSQNQKLHTELQTLKRRDCNETGTVISLKKETD 238
>TC229638 similar to UP|Q9EMG2 (Q9EMG2) AMV244, partial (11%)
Length = 780
Score = 28.5 bits (62), Expect = 2.6
Identities = 21/72 (29%), Positives = 37/72 (51%), Gaps = 8/72 (11%)
Frame = +1
Query: 8 ENNDDRRKKKKKKTETTGSEQLR--------FFVNEFQSANDVQLSSIELESLKADSSIL 59
+++ D RK+K K+ + G ++ + +V E + +VQL +ELES++
Sbjct: 286 QSSKDVRKQKAKQHKMCGGQKNKHNSVAHRGIWVKE-KWFKEVQLDQMELESIRTRGMCC 462
Query: 60 ELSNSQNSRDTD 71
L Q+ RDTD
Sbjct: 463 VLGIIQSWRDTD 498
>CO984131
Length = 896
Score = 28.5 bits (62), Expect = 2.6
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = -1
Query: 150 ISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQV 183
+SL +R N+ PS+I KL +E+L LSR Q+
Sbjct: 713 VSLNLSRNNLIGKIPSKIGKLTSLESLDLSRNQL 612
>TC226496 homologue to UP|Q93VJ8 (Q93VJ8) AT5g11200/F2I11_90
(AT5g11170/F2I11_60), partial (98%)
Length = 1674
Score = 28.1 bits (61), Expect = 3.4
Identities = 22/86 (25%), Positives = 39/86 (44%), Gaps = 3/86 (3%)
Frame = +1
Query: 105 PGSPSVLIVSPSALRSIHLLKGFRFMTKQCSAVK--LFSKHIKLQEQISLLKNRV-NIAS 161
PG + L++ + + + F + +K +F + ++ LLKN +I
Sbjct: 382 PGQVAALVLCHTRELAYQICHEFERFSTYLPDIKAAVFYGGVNIKVHKELLKNECPHIVV 561
Query: 162 GTPSRIKKLIDVEALGLSRLQVLVLD 187
GTP RI L + LGL ++ +LD
Sbjct: 562 GTPGRILALARDKDLGLKNVRHFILD 639
>TC206686 similar to UP|Q9SCP7 (Q9SCP7) Caffeic acid O-methyltransferase-like
protein (AT3g53140/T4D2_70), partial (89%)
Length = 1298
Score = 28.1 bits (61), Expect = 3.4
Identities = 15/47 (31%), Positives = 27/47 (56%)
Frame = +1
Query: 167 IKKLIDVEALGLSRLQVLVLDMHPDVKGYSLFTLPQVRCASLSSPYL 213
++KL+DV G L+ ++L+ HP +K F LP+V + P++
Sbjct: 568 VEKLVDVGGSGGDCLR-MILEKHPTIKEGINFDLPEVVAKAPQIPFV 705
>AW706707
Length = 267
Score = 28.1 bits (61), Expect = 3.4
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = -1
Query: 14 RKKKKKKTETTGSEQLRFFV 33
+KKKKKKTET +QLRFF+
Sbjct: 153 KKKKKKKTET---KQLRFFL 103
>TC213538 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase complex
H-protein, partial (15%)
Length = 727
Score = 27.7 bits (60), Expect = 4.4
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +2
Query: 1 MGKRKGRENNDDRRKKKKKKTETTGSE 27
M + KG+E R+KKKK+K E T E
Sbjct: 431 MKQSKGKEKTYHRKKKKKRKEEITVRE 511
>TC221372 homologue to UP|Q6L3T9 (Q6L3T9) NAM (No apical meristem)-like
protein-related, partial (24%)
Length = 588
Score = 27.3 bits (59), Expect = 5.8
Identities = 18/57 (31%), Positives = 29/57 (50%)
Frame = +1
Query: 57 SILELSNSQNSRDTDLDVKLLGGDIKGAFGNCWRQVLCESEVVEGKIPPGSPSVLIV 113
S+L S+ +SR L LLG + F CW ++L ++ + + S S+LIV
Sbjct: 76 SLLASSSFSSSRVLLLVQNLLGHLFQSTFKGCWLKILRRQQIKDSSL---SFSILIV 237
>TC224933 UP|Q6K853 (Q6K853) 40S ribosomal protein S30-like, complete
Length = 935
Score = 27.3 bits (59), Expect = 5.8
Identities = 15/53 (28%), Positives = 26/53 (48%)
Frame = +3
Query: 3 KRKGRENNDDRRKKKKKKTETTGSEQLRFFVNEFQSANDVQLSSIELESLKAD 55
+RK ++ ++KKKKKK ET+ R + V++ + SL+ D
Sbjct: 531 QRKKKKKKKKKKKKKKKKLETSSLSSCRIRHEDMLKYYQVEIDEL-YRSLRGD 686
>BE346992 homologue to GP|22000958|gb| DNA polymerase processivity factor
{Bovine herpesvirus 2}, partial (2%)
Length = 477
Score = 27.3 bits (59), Expect = 5.8
Identities = 17/66 (25%), Positives = 32/66 (47%)
Frame = +1
Query: 123 LLKGFRFMTKQCSAVKLFSKHIKLQEQISLLKNRVNIASGTPSRIKKLIDVEALGLSRLQ 182
LLKG + S ++ + L + +L+N + +S P+R +++ + AL L
Sbjct: 241 LLKGLLSESDWLSKAHPLNEEVSLLSEYKILENVESESSAQPTRPARMLSLLALSAQPLI 420
Query: 183 VLVLDM 188
+ LDM
Sbjct: 421 LAQLDM 438
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.134 0.373
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,058,577
Number of Sequences: 63676
Number of extensions: 118303
Number of successful extensions: 3227
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 1259
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2317
length of query: 227
length of database: 12,639,632
effective HSP length: 94
effective length of query: 133
effective length of database: 6,654,088
effective search space: 884993704
effective search space used: 884993704
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149471.20