BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149127.3 + phase: 0
(218 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230860 141 2e-34
TC230861 107 5e-24
TC211073 weakly similar to UP|BUD8_YEAST (P41698) Bud site selec... 51 5e-07
TC208859 49 2e-06
TC204920 similar to UP|PPF1_PEA (Q9FY06) Inner membrane protein ... 38 0.004
TC224693 similar to UP|Q39835 (Q39835) Extensin, partial (29%) 34 0.045
TC216969 similar to UP|Q9SHJ8 (Q9SHJ8) F12K11.12, partial (70%) 33 0.13
TC221265 33 0.13
TC223651 33 0.13
TC211491 similar to UP|Q9FMN6 (Q9FMN6) N-hydroxycinnamoyl/benzoy... 32 0.22
AI441452 32 0.22
TC221014 homologue to UP|Q6JCU4 (Q6JCU4) ATP synthase F0 subunit... 32 0.22
TC214652 homologue to UP|Q39860 (Q39860) GTPase, complete 32 0.29
TC229612 32 0.29
TC223593 similar to GB|AAN28762.1|23505805|AY143823 At1g71970/F1... 32 0.29
TC204239 UP|CAT4_SOYBN (O48561) Catalase 4 , partial (30%) 31 0.38
BM308028 homologue to GP|16973451|gb| phragmoplast-associated ki... 31 0.50
TC212421 30 0.65
TC216868 homologue to UP|Q7Y0Y9 (Q7Y0Y9) Dehydration responsive ... 30 0.65
TC211919 weakly similar to UP|K2C1_HUMAN (P04264) Keratin, type ... 30 0.65
>TC230860
Length = 456
Score = 141 bits (356), Expect = 2e-34
Identities = 83/149 (55%), Positives = 99/149 (65%), Gaps = 1/149 (0%)
Frame = +2
Query: 13 DELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRSCHTRPEL 72
D S++ P KLSL +++RP E TPPP+ T IS+PFKWEEAPGKPR CHT E
Sbjct: 14 DNKCSDHHVPTKLSL--LISRPTMPPE--TPPPQNT-ISVPFKWEEAPGKPRHCHTESE- 175
Query: 73 REREVNNVVRALELPPRLLSL-ERESTGNIYAPSPTTVLEGPYVGRAVSFTTSYRDNNNK 131
E N V + LELPPRLL L + ++ PSP TVL+GPYVGRA+SFTTSYR +
Sbjct: 176 PEDSGNAVKKTLELPPRLLFLLDTNKVSDVDGPSPITVLDGPYVGRAMSFTTSYRTPRDY 355
Query: 132 DSVNFGSSRWGGLKKNNRIDREGSFDFSS 160
+ NFGSSRW G KK D EG+FDFSS
Sbjct: 356 WNSNFGSSRWTGYKKITTED-EGTFDFSS 439
>TC230861
Length = 503
Score = 107 bits (266), Expect = 5e-24
Identities = 63/128 (49%), Positives = 77/128 (59%), Gaps = 2/128 (1%)
Frame = +1
Query: 1 MTSENNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAP 60
M SE+ DD + + KLS +++RP E PP+ T IS+PFKWEEAP
Sbjct: 133 MVSESENNKCSDDHVLT------KLSF--LISRPTMPPETLMSPPQNT-ISVPFKWEEAP 285
Query: 61 GKPRSCHTRPELREREVNNVVRALELPPRLLSL--ERESTGNIYAPSPTTVLEGPYVGRA 118
GKPR CHT + E V + LELPPRLL L ++ PSPTTVL+GPYVGRA
Sbjct: 286 GKPRHCHTESD-PEDSTTAVKKTLELPPRLLFLLDSTNKVSDVDGPSPTTVLDGPYVGRA 462
Query: 119 VSFTTSYR 126
+SFTTSYR
Sbjct: 463 MSFTTSYR 486
>TC211073 weakly similar to UP|BUD8_YEAST (P41698) Bud site selection protein
BUD8, partial (4%)
Length = 651
Score = 50.8 bits (120), Expect = 5e-07
Identities = 31/88 (35%), Positives = 41/88 (46%), Gaps = 5/88 (5%)
Frame = +3
Query: 29 TILTRPGFSSEAP-----TPPPRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVVRA 83
+I P FS ++P PP T+ S+PF+WE+ PGKPR C +
Sbjct: 81 SIARLPLFSIQSPERSGMATPPLHTLASVPFRWEQVPGKPRPCSALVSFSN------PKC 242
Query: 84 LELPPRLLSLERESTGNIYAPSPTTVLE 111
LELPPRL + PSP TV +
Sbjct: 243 LELPPRL-----------FTPSPATVFK 293
>TC208859
Length = 858
Score = 48.9 bits (115), Expect = 2e-06
Identities = 47/148 (31%), Positives = 65/148 (43%), Gaps = 12/148 (8%)
Frame = +3
Query: 29 TILTRPGFSSEAP-------TPPPRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVV 81
+I P FS ++P PP T+ S+PF+WE+ PGKPR T + +
Sbjct: 51 SIARLPLFSIQSPETERSGMATPPLETLASVPFRWEQVPGKPRP-STALVPFSNPPDLLP 227
Query: 82 RALELPPRLL-----SLERESTGNIYAPSPTTVLEGPYVGRAVSFTTSYRDNNNKDSVNF 136
+ LELPPRLL ++ + ST N Y S T +L + +A D F
Sbjct: 228 KCLELPPRLLIPSPATVFKVSTNNCYG-SDTKLLGAMVLTKA------------NDYCWF 368
Query: 137 GSSRWGGLKKNNRIDREGSFDFSSWSVE 164
GS R K N R S F S S++
Sbjct: 369 GSWRKKAFKLNKREVTGASHVFPSSSLD 452
>TC204920 similar to UP|PPF1_PEA (Q9FY06) Inner membrane protein PPF-1,
chloroplast precursor (Post-floral-specific protein 1),
partial (77%)
Length = 1245
Score = 37.7 bits (86), Expect = 0.004
Identities = 25/74 (33%), Positives = 37/74 (49%), Gaps = 1/74 (1%)
Frame = +2
Query: 34 PGFSSEAPTPPPRTTVISIPFKWEEAPGKPRSCHTR-PELREREVNNVVRALELPPRLLS 92
P SS A T P++T + E+ +P++ TR P LR R ++R L LPP +L
Sbjct: 158 PPKSSSASTKFPQSTP*AATLTSPES*LEPKAFSTRSPMLRLRRTLRLLRTLRLPPPMLP 337
Query: 93 LERESTGNIYAPSP 106
R G+ +P P
Sbjct: 338 YRRAEAGSASSPKP 379
>TC224693 similar to UP|Q39835 (Q39835) Extensin, partial (29%)
Length = 1249
Score = 34.3 bits (77), Expect = 0.045
Identities = 22/83 (26%), Positives = 40/83 (47%), Gaps = 2/83 (2%)
Frame = +1
Query: 25 LSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRS--CHTRPELREREVNNVVR 82
LSLP+ ++ + +P PP + +S P+ + P P+ + P L+ NV+
Sbjct: 103 LSLPSQISAHNYLYSSPPPPKKYPPVSPPYHYPSPPPPPKKPYYYHSPPLQIPSTTNVIA 282
Query: 83 ALELPPRLLSLERESTGNIYAPS 105
+ LP R E +T +I +P+
Sbjct: 283 STSLPLR----EPSTTFSISSPT 339
>TC216969 similar to UP|Q9SHJ8 (Q9SHJ8) F12K11.12, partial (70%)
Length = 969
Score = 32.7 bits (73), Expect = 0.13
Identities = 19/64 (29%), Positives = 30/64 (46%)
Frame = +3
Query: 155 SFDFSSWSVEGGDKVKITRVKRRGSFSHGTSHFWVCLFSYFLFIFILFYISYPFMFHSLF 214
S++F+ W +++ R+ F H S FW+ SY L++ IL P F F
Sbjct: 366 SWNFNGWRCSFDGPIEVLSCNRKNCFCHS*SKFWI---SY*LWLLIL-----PLSFTRAF 521
Query: 215 RIVL 218
R +L
Sbjct: 522 RRIL 533
>TC221265
Length = 918
Score = 32.7 bits (73), Expect = 0.13
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = -3
Query: 177 RGSFSHGTSHFWVCLF-SYFLFIFILFYISYPFMFHSLFR 215
RGSFS G S FW ++F F F+LF S F LF+
Sbjct: 319 RGSFSSGGSRFWPGKS*TFFSFFFLLFLFSSSFFIVILFK 200
>TC223651
Length = 521
Score = 32.7 bits (73), Expect = 0.13
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = -3
Query: 191 LFSYFLFIFILFYISYPFMFHSLFRI 216
+ +YF ++ +LF++SYPF+F S F +
Sbjct: 93 VITYFFYLNLLFFLSYPFLFLSSFNL 16
>TC211491 similar to UP|Q9FMN6 (Q9FMN6)
N-hydroxycinnamoyl/benzoyltransferase-like protein,
partial (15%)
Length = 481
Score = 32.0 bits (71), Expect = 0.22
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +2
Query: 194 YFLFIFILFYISYPFMFHSLF 214
+FLFI I++Y S PF+FH F
Sbjct: 71 FFLFIQIIYYFSIPFLFHFYF 133
>AI441452
Length = 424
Score = 32.0 bits (71), Expect = 0.22
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +2
Query: 24 KLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKP 63
K +P +P E P P + + S+PF WEE PGKP
Sbjct: 59 KPGIPKKDWKPEPEPEVPKTPLKL-IASVPFVWEEKPGKP 175
>TC221014 homologue to UP|Q6JCU4 (Q6JCU4) ATP synthase F0 subunit 8, partial
(31%)
Length = 700
Score = 32.0 bits (71), Expect = 0.22
Identities = 17/35 (48%), Positives = 22/35 (62%)
Frame = +3
Query: 180 FSHGTSHFWVCLFSYFLFIFILFYISYPFMFHSLF 214
F H T F++CL S+ LF F F++SY F SLF
Sbjct: 192 FLHYTHLFFLCLQSHLLFFF--FFLSYLFFSLSLF 290
>TC214652 homologue to UP|Q39860 (Q39860) GTPase, complete
Length = 1004
Score = 31.6 bits (70), Expect = 0.29
Identities = 18/45 (40%), Positives = 22/45 (48%)
Frame = +1
Query: 21 TPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRS 65
TP+K L T P S AP+PPP T +S P +P P S
Sbjct: 250 TPRKSRL-RFGTPPAKSDSAPSPPPTTAALSAPSLSTTSPAAPPS 381
>TC229612
Length = 684
Score = 31.6 bits (70), Expect = 0.29
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +1
Query: 31 LTRPGFSSEAPTP-PPRTTVISIPFKWEEAPGKP 63
+ + + EA P P + S+PF WEE PGKP
Sbjct: 58 IPKKDWKPEAEIPITPLKLIASVPFVWEEKPGKP 159
>TC223593 similar to GB|AAN28762.1|23505805|AY143823 At1g71970/F17M19_12
{Arabidopsis thaliana;} , partial (16%)
Length = 421
Score = 31.6 bits (70), Expect = 0.29
Identities = 16/49 (32%), Positives = 28/49 (56%)
Frame = +2
Query: 40 APTPPPRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVVRALELPP 88
+P P R + +PF WE PG P+ +++ +L+E + ++ L LPP
Sbjct: 185 SPASPLRFS--GVPFSWEHLPGIPKKQNSKKKLQE----SSLKLLPLPP 313
>TC204239 UP|CAT4_SOYBN (O48561) Catalase 4 , partial (30%)
Length = 861
Score = 31.2 bits (69), Expect = 0.38
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 178 GSFSHGTSHFWVCLFSYFLFIFILF 202
G + T HF CLFSY+L +FI F
Sbjct: 751 GKYLIETYHFSFCLFSYYLILFITF 825
>BM308028 homologue to GP|16973451|gb| phragmoplast-associated
kinesin-related protein 2 {Arabidopsis thaliana},
partial (1%)
Length = 366
Score = 30.8 bits (68), Expect = 0.50
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 3/46 (6%)
Frame = +1
Query: 169 VKITRVKRRG---SFSHGTSHFWVCLFSYFLFIFILFYISYPFMFH 211
V I ++KRR F G + F+ C F Y +FI F + Y F+F+
Sbjct: 199 VCIEKMKRRVLLVMFPSGNTVFFGCSFEYVYIVFIGFVVVYYFVFY 336
>TC212421
Length = 734
Score = 30.4 bits (67), Expect = 0.65
Identities = 16/63 (25%), Positives = 29/63 (45%)
Frame = -3
Query: 32 TRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPRSCHTRPELREREVNNVVRALELPPRLL 91
TR GF ++ P+T SI + + CHT EL+ N++ + ++L
Sbjct: 312 TREGFPAKFGNSTPQTPDFSIQSLIQSCTNNDKDCHTAVELQVCNPNSIGHLTLISQKIL 133
Query: 92 SLE 94
S++
Sbjct: 132 SIQ 124
>TC216868 homologue to UP|Q7Y0Y9 (Q7Y0Y9) Dehydration responsive element
binding protein, partial (53%)
Length = 1229
Score = 30.4 bits (67), Expect = 0.65
Identities = 31/118 (26%), Positives = 44/118 (37%), Gaps = 9/118 (7%)
Frame = +1
Query: 2 TSENNKKPDHDDELR--SNYDTPKKLSLPT-------ILTRPGFSSEAPTPPPRTTVISI 52
+S N K H +E S ++L PT +LT P APT PP +T ++
Sbjct: 250 SSSTNNKRSHTEE*G*GSGASGWRRLENPTRGRGSGWVLTPPPSPPHAPTTPPSSTSEAL 429
Query: 53 PFKWEEAPGKPRSCHTRPELREREVNNVVRALELPPRLLSLERESTGNIYAPSPTTVL 110
P PR+ RP R ++ + PP+ S P P T L
Sbjct: 430 PLASTSPNCCPRTTTFRP--NNRATCPPIQFAKKPPKSAPESTRSKPRFSNPRPHTPL 597
>TC211919 weakly similar to UP|K2C1_HUMAN (P04264) Keratin, type II
cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa
cytokeratin) (Hair alpha protein), partial (5%)
Length = 590
Score = 30.4 bits (67), Expect = 0.65
Identities = 19/66 (28%), Positives = 26/66 (38%)
Frame = +3
Query: 5 NNKKPDHDDELRSNYDTPKKLSLPTILTRPGFSSEAPTPPPRTTVISIPFKWEEAPGKPR 64
N P + +N TP+ LP + P +S+ PPP + S P E P P
Sbjct: 243 NFNTPTPPSPIDNNPTTPQTPQLPNPIPPPPYSNNPSLPPPPPILQSPPPPEETTPPTPP 422
Query: 65 SCHTRP 70
S P
Sbjct: 423 SVAAPP 440
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.137 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,122,417
Number of Sequences: 63676
Number of extensions: 214403
Number of successful extensions: 2756
Number of sequences better than 10.0: 137
Number of HSP's better than 10.0 without gapping: 2673
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2736
length of query: 218
length of database: 12,639,632
effective HSP length: 93
effective length of query: 125
effective length of database: 6,717,764
effective search space: 839720500
effective search space used: 839720500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149127.3