BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149050.9 - phase: 0 /pseudo
(157 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209432 similar to UP|Q93Z42 (Q93Z42) AT5g19750/T29J13_170, par... 192 2e-49
TC209954 similar to UP|Q9DUC1 (Q9DUC1) ORF1, partial (3%) 95 1e-20
TC228157 similar to UP|Q6NMZ6 (Q6NMZ6) At3g24570, partial (82%) 61 2e-10
TC225285 similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRANE PROT... 58 2e-09
TC229695 57 4e-09
BM528142 50 3e-07
TC218627 50 6e-07
TC225289 weakly similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRA... 48 2e-06
AW759809 38 0.002
TC229694 28 0.015
CK605652 29 0.82
TC219788 29 1.1
BG650799 28 1.8
BG510043 similar to GP|15146316|gb AT5g67220/K21H1_18 {Arabidops... 28 1.8
BQ473897 similar to GP|571484|gb|A peroxidase precursor {Medicag... 28 1.8
AW782136 homologue to GP|21553745|gb| Yippee-like protein {Arabi... 28 2.4
TC204255 PDB|1OD5_A.0|33357661|1OD5_A Chain A, Crystal Structure... 28 2.4
BQ786062 27 5.3
TC233105 27 5.3
BF596427 27 5.3
>TC209432 similar to UP|Q93Z42 (Q93Z42) AT5g19750/T29J13_170, partial (47%)
Length = 617
Score = 192 bits (487), Expect(2) = 2e-49
Identities = 90/105 (85%), Positives = 98/105 (92%)
Frame = +2
Query: 48 RTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLV 107
R F+FLG LVGPTLHFWYLYLS+LVTLPG SGA+LRLVLDQF+FSPIF+GVFLS+LV
Sbjct: 47 RXXXFTFLGFALVGPTLHFWYLYLSKLVTLPGASGALLRLVLDQFLFSPIFIGVFLSTLV 226
Query: 108 TLEGRPSQAVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
TLEG PS+AVPKLKQEWFSAVLANW+LWIPFQFLNFRFVPQQFQV
Sbjct: 227 TLEGNPSRAVPKLKQEWFSAVLANWKLWIPFQFLNFRFVPQQFQV 361
Score = 20.4 bits (41), Expect(2) = 2e-49
Identities = 8/9 (88%), Positives = 9/9 (99%)
Frame = +3
Query: 32 LICQLVIDK 40
LICQLVID+
Sbjct: 3 LICQLVIDQ 29
>TC209954 similar to UP|Q9DUC1 (Q9DUC1) ORF1, partial (3%)
Length = 610
Score = 95.1 bits (235), Expect = 1e-20
Identities = 46/59 (77%), Positives = 52/59 (87%)
Frame = +2
Query: 7 YMALLAKYPVPVKALTSAILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLH 65
Y+ALL KYPV VKALTS+ILNLIGDLICQLVID+V + D KRTF+F+FLG LVGPTLH
Sbjct: 434 YLALLGKYPVAVKALTSSILNLIGDLICQLVIDQVPSLDFKRTFVFTFLGFALVGPTLH 610
>TC228157 similar to UP|Q6NMZ6 (Q6NMZ6) At3g24570, partial (82%)
Length = 1110
Score = 61.2 bits (147), Expect = 2e-10
Identities = 47/173 (27%), Positives = 79/173 (45%), Gaps = 21/173 (12%)
Frame = +1
Query: 1 MTLYYRYMALLAKYPVPVKALTSAILNLIGDLICQLV----------IDKVQTPDLK--- 47
+ L+ Y LA +PV + ++S ++ GD+ Q V D T + K
Sbjct: 271 LRLWKWYQNCLAVHPVKTQVISSGLIWGAGDIAAQAVTHYTAKKRVTFDADDTKEFKINW 450
Query: 48 -RTFLFSFLGLVLVGPTLHFWYLYLSQLVTL-----PGTSGAI-LRLVLDQFVFSPIFLG 100
R S GL VGP HFWY L + + L P + + ++ +D F+F P+ L
Sbjct: 451 RRVSTTSLFGLGFVGPVGHFWYEGLDRFIRLKLMLKPNSFRFVATKVAVDGFIFGPLDLL 630
Query: 101 VFLSSLVTLEGRP-SQAVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
VF + + G+ Q +K+++ A + +W Q NFRF+P ++Q+
Sbjct: 631 VFFTYMGFSAGKSVPQVKEDVKRDFLPAFVLEGGIWPIVQVANFRFIPVRYQL 789
>TC225285 similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRANE PROTEIN PMP22
(Pmp22 peroxisomal membrane protein) (At4g04470),
partial (88%)
Length = 834
Score = 58.2 bits (139), Expect = 2e-09
Identities = 40/149 (26%), Positives = 77/149 (50%), Gaps = 3/149 (2%)
Frame = +3
Query: 7 YMALLAKYPVPVKALTSAILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLHF 66
Y+ L ++P+ K +T+ +L+ I D++ Q + +Q LKR G +GP HF
Sbjct: 111 YVKQLQQHPLRTKVITAGVLSAISDVVSQKLTG-IQKLQLKRLLFKVIFGAAYLGPFGHF 287
Query: 67 WYLYLSQLVTLPGTSGAILRLVL-DQFVFSPIFLGVFLSSL-VTLEGRPSQAV-PKLKQE 123
++L L ++ S + + VL +Q +P +F+ + +EG+P V K+K++
Sbjct: 288 FHLILDKIFKGKRDSKTVAKKVLIEQLTSNPWNNLLFMIYYGLVVEGQPWVNVKAKVKKD 467
Query: 124 WFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
+ S +W +W ++N +F+P F+V
Sbjct: 468 YPSVQYTSWTVWPVVGWINHKFMPLHFRV 554
>TC229695
Length = 639
Score = 57.0 bits (136), Expect = 4e-09
Identities = 44/158 (27%), Positives = 67/158 (41%), Gaps = 19/158 (12%)
Frame = +2
Query: 14 YPVPVK-ALTSAILNLIGDLICQL------------------VIDKVQTPDLKRTFLFSF 54
Y PVK A+T+A L L GD I QL + + D R +
Sbjct: 116 YRFPVKQAVTAASLALTGDTIAQLSHRWRKAKEGGGSVSQDELWRYLSDHDWLRALRMTS 295
Query: 55 LGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRPS 114
G +L GP + WY L + P +L++VL+Q V P + V + + S
Sbjct: 296 YGFLLYGPGSYAWYQCLDHCLPKPTVQNLVLKVVLNQIVLGPCVIAVVFAWNNLWLQKLS 475
Query: 115 QAVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
+ K +++ +L ++ WIP LNF VP Q +V
Sbjct: 476 ELPEKYRRDALPTLLYGFRFWIPVSVLNFWVVPLQARV 589
>BM528142
Length = 427
Score = 50.4 bits (119), Expect = 3e-07
Identities = 32/104 (30%), Positives = 50/104 (47%), Gaps = 5/104 (4%)
Frame = +3
Query: 5 YRYMALLAKYPVPVKALTSAILNLIGDLICQLVIDK--VQTPDLKRTFLFSFLGLVLVGP 62
YRY L K+PV K +T + D++CQL+ ++ +QT RT G V P
Sbjct: 48 YRYSESLRKHPVYTKMMTIGGIYFTADVLCQLLKERKSLQTYSTGRTLRHLVNGTFFVAP 227
Query: 63 TLHFWYLYLSQLVTLPGTS---GAILRLVLDQFVFSPIFLGVFL 103
TLH W+ + +T + + + + VF+P+FLG L
Sbjct: 228 TLHVWHSKVIPALTYAFKNKYKKILCAYLFGEIVFAPLFLGSLL 359
>TC218627
Length = 1021
Score = 49.7 bits (117), Expect = 6e-07
Identities = 38/137 (27%), Positives = 59/137 (42%), Gaps = 2/137 (1%)
Frame = +1
Query: 7 YMALLAKYPVPVKALTSAILNLIGDLICQLVIDKVQTP-DLKRTFLFSFLGLVLVGPTLH 65
Y L PV K + S I+ IGD I Q K D R F +G L G H
Sbjct: 217 YEEALKTNPVLAKMMISGIVYSIGDWIAQCFEGKPLFEFDRARMFRSGLVGFTLHGSLSH 396
Query: 66 FWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRPSQAV-PKLKQEW 124
F+Y + +L ++ DQ +S ++ ++ + + L P ++ +LK +
Sbjct: 397 FYYQFCEELFPYKEWWVVPAKVAFDQTAWSALWNSIYYTVVALLRRDPPMSILNELKATF 576
Query: 125 FSAVLANWQLWIPFQFL 141
F + A W+LW PF L
Sbjct: 577 FPMLTAGWKLW-PFAHL 624
>TC225289 weakly similar to UP|Q9ZS51 (Q9ZS51) PEROXISOMAL MEMBRANE PROTEIN
PMP22 (Pmp22 peroxisomal membrane protein) (At4g04470),
partial (86%)
Length = 818
Score = 48.1 bits (113), Expect = 2e-06
Identities = 35/131 (26%), Positives = 66/131 (49%), Gaps = 3/131 (2%)
Frame = +1
Query: 25 ILNLIGDLICQLVIDKVQTPDLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAI 84
+L+ I D++ Q + +Q LKR G +GP HF++L L ++ S +
Sbjct: 127 VLSAISDVVSQKLTG-IQKLQLKRLLFKVIFGAAYLGPFGHFFHLILDKIFKGKRDSKTV 303
Query: 85 LRLVL-DQFVFSPIFLGVFLSSL-VTLEGRPSQAV-PKLKQEWFSAVLANWQLWIPFQFL 141
+ VL +Q +P +F+ + +EG+P V K+K+++ S +W +W ++
Sbjct: 304 AKKVLIEQLTSNPWNNLLFMIYYGLVVEGQPWVNVKAKVKKDYLSVQYTSWTVWPVVGWI 483
Query: 142 NFRFVPQQFQV 152
N +F+P F+V
Sbjct: 484 NHKFMPLHFRV 516
>AW759809
Length = 398
Score = 38.1 bits (87), Expect = 0.002
Identities = 27/88 (30%), Positives = 40/88 (44%), Gaps = 13/88 (14%)
Frame = +3
Query: 1 MTLYYRYMALLAKYPVPVKALTSAILNLIGDLICQLVI-------------DKVQTPDLK 47
M ++ Y L+ +PV +A++SAIL +G L Q + D T +
Sbjct: 78 MKVWNWYQNCLSVHPVKTQAISSAILWGVGYLSAQYITHSAAKKPLQLSDSDAKFTINWN 257
Query: 48 RTFLFSFLGLVLVGPTLHFWYLYLSQLV 75
R + S G LVGP HFWY L + +
Sbjct: 258 RLVVTSMFGFGLVGPVGHFWYEGLDKCI 341
>TC229694
Length = 899
Score = 28.1 bits (61), Expect(2) = 0.015
Identities = 13/39 (33%), Positives = 21/39 (53%)
Frame = +3
Query: 114 SQAVPKLKQEWFSAVLANWQLWIPFQFLNFRFVPQQFQV 152
S+ K +++ +L ++ WIP LNF VP Q +V
Sbjct: 360 SELPEKYRRDALPTLLYGFRFWIPVSVLNFWVVPLQARV 476
Score = 25.8 bits (55), Expect(2) = 0.015
Identities = 15/62 (24%), Positives = 26/62 (41%)
Frame = +1
Query: 45 DLKRTFLFSFLGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLS 104
D R + G +L GP + WY L + P +L++ + +F + + S
Sbjct: 178 DWLRALRMTSYGFLLYGPGSYAWYQCLDHCLPKPTVQNLVLKVHVSLLLFLHGIIYGYRS 357
Query: 105 SL 106
SL
Sbjct: 358 SL 363
>CK605652
Length = 282
Score = 29.3 bits (64), Expect = 0.82
Identities = 15/38 (39%), Positives = 21/38 (54%), Gaps = 1/38 (2%)
Frame = -2
Query: 66 FWYLYLSQLVTLPGTSGAILR-LVLDQFVFSPIFLGVF 102
+W + +L S A+LR L FVF+PIF G+F
Sbjct: 242 YWSSFSRVSFSLRSVSTALLRALAASAFVFTPIFFGIF 129
>TC219788
Length = 759
Score = 28.9 bits (63), Expect = 1.1
Identities = 22/88 (25%), Positives = 36/88 (40%), Gaps = 1/88 (1%)
Frame = +1
Query: 55 LGLVLVGPTLHFWYLYLSQLVTLPGTSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRP- 113
+G L G H++Y L ++ DQ V+S I+ ++ L L
Sbjct: 16 VGFTLHGSLSHYYYQLCEALFPFQEWWVVPAKVAFDQTVWSAIWNSIYFVVLGLLRFESL 195
Query: 114 SQAVPKLKQEWFSAVLANWQLWIPFQFL 141
+ +LK + + A W+LW PF L
Sbjct: 196 TNIYGELKSTFLPLLTAGWKLW-PFAHL 276
>BG650799
Length = 381
Score = 28.1 bits (61), Expect = 1.8
Identities = 15/57 (26%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +3
Query: 86 RLVLDQFVFSPIFLGVFLSSLVTLEGRPSQAV-PKLKQEWFSAVLANWQLWIPFQFL 141
++ DQ +S ++ ++ + + L P ++ +LK +F + A W+LW PF L
Sbjct: 132 KVAFDQTAWSALWNSIYYTVVALLRLDPPMSILNELKATFFPMLTAGWKLW-PFAHL 299
>BG510043 similar to GP|15146316|gb AT5g67220/K21H1_18 {Arabidopsis
thaliana}, partial (25%)
Length = 425
Score = 28.1 bits (61), Expect = 1.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 3 LYYRYMALLAKYPVPVKALTSAILNLIGD 31
L Y+ L KYPVP + + S + L+GD
Sbjct: 147 LLIEYLKLCEKYPVPWRMIRSHVHKLLGD 233
>BQ473897 similar to GP|571484|gb|A peroxidase precursor {Medicago
truncatula}, partial (34%)
Length = 421
Score = 28.1 bits (61), Expect = 1.8
Identities = 18/45 (40%), Positives = 24/45 (53%)
Frame = -1
Query: 80 TSGAILRLVLDQFVFSPIFLGVFLSSLVTLEGRPSQAVPKLKQEW 124
TS ++ L+ + VFSP+ LGV S + PSQ P KQ W
Sbjct: 337 TSKPLIELIFGRAVFSPVKLGVSSSRI-----DPSQ--PLTKQSW 224
>AW782136 homologue to GP|21553745|gb| Yippee-like protein {Arabidopsis
thaliana}, partial (10%)
Length = 396
Score = 27.7 bits (60), Expect = 2.4
Identities = 12/22 (54%), Positives = 17/22 (76%), Gaps = 2/22 (9%)
Frame = +3
Query: 48 RTFLFSFLGLVLVGPT--LHFW 67
++F+FSF+ L+LVGP L FW
Sbjct: 96 KSFMFSFITLLLVGPNSKLSFW 161
>TC204255 PDB|1OD5_A.0|33357661|1OD5_A Chain A, Crystal Structure Of Glycinin
A3b4 Subunit Homohexamer. {Glycine max;} , partial (6%)
Length = 519
Score = 27.7 bits (60), Expect = 2.4
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 4/33 (12%)
Frame = -3
Query: 46 LKRTFLFSFL---GL-VLVGPTLHFWYLYLSQL 74
L+ F FS L GL V +GPT H W ++L++L
Sbjct: 475 LESVFFFSKLWTPGLPVAMGPTCHMWLMFLAEL 377
>BQ786062
Length = 406
Score = 26.6 bits (57), Expect = 5.3
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +3
Query: 112 RPSQAVPKLKQEWFSAVLANWQLW 135
RP+++ KL ++W +LA+ Q+W
Sbjct: 141 RPTESAKKLAKQWKEMLLADDQIW 212
>TC233105
Length = 586
Score = 26.6 bits (57), Expect = 5.3
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -3
Query: 75 VTLPGTSGAILRLVLDQFVFSPIFLGVFLSSL 106
VTL TSG+IL + F+ +FL + + SL
Sbjct: 317 VTLASTSGSILLALQQSSEFASLFLRIIIDSL 222
>BF596427
Length = 409
Score = 26.6 bits (57), Expect = 5.3
Identities = 10/28 (35%), Positives = 13/28 (45%)
Frame = -3
Query: 110 EGRPSQAVPKLKQEWFSAVLANWQLWIP 137
EG P + P+LK WF W+P
Sbjct: 125 EGPPRKQAPRLKPRWFPKSSTETWTWMP 42
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.332 0.146 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,583,701
Number of Sequences: 63676
Number of extensions: 109538
Number of successful extensions: 844
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 836
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 839
length of query: 157
length of database: 12,639,632
effective HSP length: 89
effective length of query: 68
effective length of database: 6,972,468
effective search space: 474127824
effective search space used: 474127824
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149050.9