BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149050.15 + phase: 0
(235 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205003 similar to GB|AAS20952.1|42565013|AY531115 calmodulin b... 236 9e-63
TC220856 similar to GB|AAS20952.1|42565013|AY531115 calmodulin b... 131 2e-31
TC205006 similar to GB|AAS20952.1|42565013|AY531115 calmodulin b... 87 5e-18
BI970801 75 3e-14
TC229170 59 2e-09
TC229171 49 2e-06
TC220293 49 2e-06
TC228184 46 2e-05
BG405443 43 1e-04
TC211369 43 1e-04
TC212907 37 0.008
TC234499 weakly similar to UP|Q94A53 (Q94A53) At1g12830/F13K23_6... 36 0.017
TC231797 similar to UP|Q9FZA1 (Q9FZA1) F3H9.7 protein, partial (... 34 0.066
CK769131 33 0.11
AI960729 similar to GP|21593313|gb| protein disulfide isomerase ... 32 0.33
BM527146 similar to GP|18491265|gb| At2g46160/T3F17.19 {Arabidop... 32 0.33
TC209061 weakly similar to UP|Q9FNP0 (Q9FNP0) Gb|AAF27124.1, par... 32 0.33
TC208606 similar to PIR|T02655|T02655 hydroxymethylglutaryl-CoA ... 32 0.33
BQ452938 31 0.43
TC216636 similar to UP|Q8S0V8 (Q8S0V8) OJ1014_G12.16 protein, pa... 31 0.43
>TC205003 similar to GB|AAS20952.1|42565013|AY531115 calmodulin binding
protein 25 {Arabidopsis thaliana;} , partial (24%)
Length = 1133
Score = 236 bits (601), Expect = 9e-63
Identities = 154/250 (61%), Positives = 180/250 (71%), Gaps = 15/250 (6%)
Frame = +2
Query: 1 MASSENLAASIESWMYRPAMTDTWLSDYISRDAETLTKALHKSLSSSSSP--EDALSPFL 58
MASSEN+A SIE M RP +TD+WL+++ +RDA+ LTKAL KSLS + P E+ SPFL
Sbjct: 110 MASSENVA-SIEPCMLRPPVTDSWLAEFFARDADALTKALQKSLSGTVLPDEENYFSPFL 286
Query: 59 NLIKTDSATTTTTTTPTVSSLSASD-DSAPKRQRVA--AGKISKRKSRASKRSQTTFITA 115
K+D A T PTVSSLS SD DSAP+R+ +GKISKRKSRASKRSQTTFITA
Sbjct: 287 P--KSDPAVAT----PTVSSLSGSDQDSAPRRRAPLPPSGKISKRKSRASKRSQTTFITA 448
Query: 116 DPANFRQMVQQVTGVRFGSGSNVSMAP---LVKPEPHRAVGVNGGGRFTTGGGCLPTLDT 172
DPANFRQMVQQVTGVRFG G+ +AP +VKPEP RA G+ RF G GCLPTLDT
Sbjct: 449 DPANFRQMVQQVTGVRFG-GAGQPIAPAAGVVKPEPQRASGL---ARFPAGAGCLPTLDT 616
Query: 173 SAFLLQHHQQQQQTMVGPNS--DGPE--MTGLGPLSFGQPIGE---DAAGYDFETFSSCF 225
SAFLL HHQQ +VGP+S GP ++G GPL F QP+ + +AG DF+TFSS F
Sbjct: 617 SAFLLDHHQQ----VVGPSSVNSGPATGLSGPGPLPFAQPLLDASFASAGLDFDTFSS-F 781
Query: 226 PTLESSWKVM 235
PTLE SWKVM
Sbjct: 782 PTLE-SWKVM 808
>TC220856 similar to GB|AAS20952.1|42565013|AY531115 calmodulin binding
protein 25 {Arabidopsis thaliana;} , partial (11%)
Length = 428
Score = 131 bits (330), Expect = 2e-31
Identities = 75/124 (60%), Positives = 91/124 (72%), Gaps = 4/124 (3%)
Frame = +1
Query: 1 MASSENLAASIESWMYRPAMTDTWLSDYISRDAETLTKALHKSLSSSSSPED-ALSPFLN 59
MASS+N+A SIE WM RP +TD+WL+++ +RDA+TLT+AL KSLS + P+D LSPFL+
Sbjct: 76 MASSQNVA-SIEPWMLRPPVTDSWLAEFFARDADTLTRALQKSLSGAVLPQDNHLSPFLH 252
Query: 60 LIKTDSATTTTTTTPTVSSLSASDDSAPKRQRVA---AGKISKRKSRASKRSQTTFITAD 116
TPTVSSLS SD + R+R A +GKISKRKSRASKRSQTTFITAD
Sbjct: 253 ------QPAAAAATPTVSSLSGSDQESAPRRRAAPPPSGKISKRKSRASKRSQTTFITAD 414
Query: 117 PANF 120
PANF
Sbjct: 415 PANF 426
>TC205006 similar to GB|AAS20952.1|42565013|AY531115 calmodulin binding
protein 25 {Arabidopsis thaliana;} , partial (13%)
Length = 742
Score = 87.4 bits (215), Expect = 5e-18
Identities = 52/107 (48%), Positives = 66/107 (61%), Gaps = 6/107 (5%)
Frame = +1
Query: 135 GSNVSMAPLVKPEPHRAVGVNGGGRFTTGGGCLPTLDTSAFLLQHHQQQQQTMVGPNSDG 194
G+ ++ A +VKPEP R G+ RF G GCLPTLDTSAFLL HHQ + G
Sbjct: 4 GAPIAPAAVVKPEPQRXSGL---ARFPAGVGCLPTLDTSAFLLDHHQXXVVPASVNSGPG 174
Query: 195 PEMTGLGPLSFGQPIGE------DAAGYDFETFSSCFPTLESSWKVM 235
++G GPL F QP+ + ++G DF+TFS+ FPTLE SWKVM
Sbjct: 175 TGLSGPGPLPFAQPLLDAHAASFASSGLDFDTFSN-FPTLE-SWKVM 309
>BI970801
Length = 420
Score = 74.7 bits (182), Expect = 3e-14
Identities = 47/95 (49%), Positives = 57/95 (59%), Gaps = 7/95 (7%)
Frame = -1
Query: 143 LVKPEPHRAVGVNGGGRFTTGGGCLPTLDTSAFLLQHHQQQQQTMVGPNS----DGPEMT 198
+VKPEP RA G+ RF G GCL TLD SAFL+ HHQQ +VGP+S P +
Sbjct: 420 VVKPEPQRASGL---ARFPAGAGCLRTLDPSAFLVGHHQQ----VVGPSSVNFGPAPGLF 262
Query: 199 GLGPLSFGQPIGE---DAAGYDFETFSSCFPTLES 230
G GP F QP+ + +AG DF+ FS FPT ES
Sbjct: 261 GAGPFPFAQPLLDASFASAGLDFDPFSR-FPTFES 160
>TC229170
Length = 1025
Score = 58.9 bits (141), Expect = 2e-09
Identities = 38/118 (32%), Positives = 57/118 (48%), Gaps = 3/118 (2%)
Frame = +2
Query: 15 MYRPAMTDTWLSDYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTTTP 74
M A+TD W+ Y + S AL+P + AT TTTT+
Sbjct: 68 MTMAAITDQWMQFY-----------QQPLMDSHGHDHHALAPISTELGFSDATVTTTTSS 214
Query: 75 TVSSLSASDDSAPKRQRVAAGKISK---RKSRASKRSQTTFITADPANFRQMVQQVTG 129
++ +S+ ++ Q G +SK R+SRASK++ TT + A+ NFR +VQQ TG
Sbjct: 215 PTPTMPSSESNSILSQLSPKGNVSKPIRRRSRASKKTPTTLLNANTTNFRALVQQFTG 388
>TC229171
Length = 432
Score = 49.3 bits (116), Expect = 2e-06
Identities = 29/67 (43%), Positives = 40/67 (59%), Gaps = 3/67 (4%)
Frame = +3
Query: 66 ATTTTTTTPTVSSLSASDDSAPKRQRVAAGKISK---RKSRASKRSQTTFITADPANFRQ 122
+++ T T P+ S S +PK G +SK R+SRASKR+ TT + A+P NFR
Sbjct: 189 SSSPTPTMPSSESNSILSQLSPK------GNVSKPIRRRSRASKRTPTTLLNANPTNFRA 350
Query: 123 MVQQVTG 129
+VQQ TG
Sbjct: 351 LVQQFTG 371
>TC220293
Length = 921
Score = 48.9 bits (115), Expect = 2e-06
Identities = 40/115 (34%), Positives = 50/115 (42%), Gaps = 16/115 (13%)
Frame = +1
Query: 48 SSPEDALSPFLNLIKTDS-----ATTTTTTTPTVSSLSASDD--------SAPKRQRVAA 94
S P D L F N T S TT TT V++ S D S
Sbjct: 127 SGPNDWLQ-FYNQNLTPSIPDFTVTTAAATTAIVAATSPPQDMPNLSPSGSTHLSPEGRV 303
Query: 95 GKISKRKSRASKRSQTTFITADPANFRQMVQQVTG---VRFGSGSNVSMAPLVKP 146
K ++R+SRAS+R+ TT + D NFR MVQQ TG F S ++S KP
Sbjct: 304 AKPTRRRSRASRRTPTTLLNTDTTNFRAMVQQFTGGPSAPFASNPSLSTTSFAKP 468
>TC228184
Length = 818
Score = 45.8 bits (107), Expect = 2e-05
Identities = 27/65 (41%), Positives = 34/65 (51%)
Frame = +2
Query: 65 SATTTTTTTPTVSSLSASDDSAPKRQRVAAGKISKRKSRASKRSQTTFITADPANFRQMV 124
S TT TTT + LS K +R+SRAS+R+ TT + D +NFR MV
Sbjct: 185 SDATTVTTTAAPAKLSPEG---------GVSKPIRRRSRASRRTPTTLLNTDTSNFRAMV 337
Query: 125 QQVTG 129
QQ TG
Sbjct: 338 QQFTG 352
>BG405443
Length = 339
Score = 42.7 bits (99), Expect = 1e-04
Identities = 18/32 (56%), Positives = 25/32 (77%)
Frame = +3
Query: 99 KRKSRASKRSQTTFITADPANFRQMVQQVTGV 130
K++SRAS+R+ TT +T D NFR MVQ+ TG+
Sbjct: 36 KKRSRASRRAPTTVLTTDTTNFRAMVQEFTGI 131
>TC211369
Length = 667
Score = 42.7 bits (99), Expect = 1e-04
Identities = 18/32 (56%), Positives = 25/32 (77%)
Frame = +3
Query: 99 KRKSRASKRSQTTFITADPANFRQMVQQVTGV 130
K++SRAS+R+ TT +T D NFR MVQ+ TG+
Sbjct: 96 KKRSRASRRAPTTVLTTDTTNFRAMVQEFTGI 191
>TC212907
Length = 421
Score = 37.0 bits (84), Expect = 0.008
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +2
Query: 99 KRKSRASKRSQTTFITADPANFRQMVQ 125
K++SRAS+R+ TT +T D NFR MVQ
Sbjct: 341 KKRSRASRRAPTTVLTTDTTNFRAMVQ 421
>TC234499 weakly similar to UP|Q94A53 (Q94A53) At1g12830/F13K23_6, partial
(26%)
Length = 894
Score = 35.8 bits (81), Expect = 0.017
Identities = 27/92 (29%), Positives = 41/92 (44%), Gaps = 7/92 (7%)
Frame = +2
Query: 27 DYISRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTT-------TTPTVSSL 79
D + ET+ ++ S SSSSS FL K+ S TTT P+ S +
Sbjct: 26 DSVPETRETVAAGINSS-SSSSSSSSPKP*FLQFTKSHSWLNTTTHPKNPHCPPPSASPI 202
Query: 80 SASDDSAPKRQRVAAGKISKRKSRASKRSQTT 111
S S S PK + K + + S+ + + +TT
Sbjct: 203 STSKTSLPKSPSSTSKKTTTKTSKTTMKERTT 298
>TC231797 similar to UP|Q9FZA1 (Q9FZA1) F3H9.7 protein, partial (37%)
Length = 658
Score = 33.9 bits (76), Expect = 0.066
Identities = 21/78 (26%), Positives = 37/78 (46%), Gaps = 1/78 (1%)
Frame = +1
Query: 73 TPTVSSLSASDDSAPKRQRVAAGKISKRKSRASKRSQTTFITADPANFRQMVQQVTG-VR 131
TPT ++ + S P + +S ++ TTF+ AD +F+Q+VQ +TG +
Sbjct: 88 TPTAAAAQSPTSSYPPKPVT--------RSESANPYPTTFVQADTTSFKQVVQMLTGSTQ 243
Query: 132 FGSGSNVSMAPLVKPEPH 149
++ S + KP H
Sbjct: 244 TAKQASASASEPAKPHTH 297
>CK769131
Length = 425
Score = 33.1 bits (74), Expect = 0.11
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 2/55 (3%)
Frame = +1
Query: 77 SSLSASDDSAPKRQRV--AAGKISKRKSRASKRSQTTFITADPANFRQMVQQVTG 129
S +A D +P+ Q K +S + TTF+ AD ++F+Q+VQ +TG
Sbjct: 43 SESAAMDSISPRAQENNNTTAKAMMTRSEPANPYPTTFVQADTSSFKQVVQMLTG 207
>AI960729 similar to GP|21593313|gb| protein disulfide isomerase
precursor-like {Arabidopsis thaliana}, partial (6%)
Length = 438
Score = 31.6 bits (70), Expect = 0.33
Identities = 24/72 (33%), Positives = 37/72 (51%)
Frame = +3
Query: 44 LSSSSSPEDALSPFLNLIKTDSATTTTTTTPTVSSLSASDDSAPKRQRVAAGKISKRKSR 103
LS SS P +LS +KT + TTTTTT +S+ S + + P A +I +R
Sbjct: 96 LSFSSHPSLSLSAIKPPLKTTTKKTTTTTT-KISASSRNPMTLPPLLTKAHFQIPTSSTR 272
Query: 104 ASKRSQTTFITA 115
+ ++TT T+
Sbjct: 273 TTATTRTTSATS 308
>BM527146 similar to GP|18491265|gb| At2g46160/T3F17.19 {Arabidopsis
thaliana}, partial (36%)
Length = 422
Score = 31.6 bits (70), Expect = 0.33
Identities = 22/62 (35%), Positives = 33/62 (52%), Gaps = 3/62 (4%)
Frame = +3
Query: 30 SRDAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTT---TTPTVSSLSASDDSA 86
S + + T + S SSSSSP + P + +ATTT T TTP ++S S + S+
Sbjct: 87 SPTSASATPSPSPSASSSSSPPSSSPPTSAAAPSATATTTATPPVTTPPMASSSRA*SSS 266
Query: 87 PK 88
P+
Sbjct: 267 PR 272
>TC209061 weakly similar to UP|Q9FNP0 (Q9FNP0) Gb|AAF27124.1, partial (30%)
Length = 617
Score = 31.6 bits (70), Expect = 0.33
Identities = 12/20 (60%), Positives = 18/20 (90%)
Frame = +3
Query: 110 TTFITADPANFRQMVQQVTG 129
TTF+ A+P+NFR +VQ++TG
Sbjct: 48 TTFVQANPSNFRAVVQKLTG 107
>TC208606 similar to PIR|T02655|T02655 hydroxymethylglutaryl-CoA lyase -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(36%)
Length = 1157
Score = 31.6 bits (70), Expect = 0.33
Identities = 23/80 (28%), Positives = 38/80 (46%)
Frame = +2
Query: 32 DAETLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTTTPTVSSLSASDDSAPKRQR 91
++E +A +++ SS+ A + T +ATT T TTP L D S+P +R
Sbjct: 41 NSEREKEAKNRTAQQSSAARPAA-----VSSTRTATTVTHTTPAHDVLERDDGSSPTARR 205
Query: 92 VAAGKISKRKSRASKRSQTT 111
+ K+ +R+QTT
Sbjct: 206 TTHAR-DPSKTHPRERTQTT 262
>BQ452938
Length = 425
Score = 31.2 bits (69), Expect = 0.43
Identities = 14/27 (51%), Positives = 19/27 (69%)
Frame = +1
Query: 103 RASKRSQTTFITADPANFRQMVQQVTG 129
RASK + T + A+ +NFR +VQQ TG
Sbjct: 1 RASKSTPITLLKANTSNFRALVQQFTG 81
>TC216636 similar to UP|Q8S0V8 (Q8S0V8) OJ1014_G12.16 protein, partial (8%)
Length = 633
Score = 31.2 bits (69), Expect = 0.43
Identities = 25/85 (29%), Positives = 38/85 (44%)
Frame = -1
Query: 35 TLTKALHKSLSSSSSPEDALSPFLNLIKTDSATTTTTTTPTVSSLSASDDSAPKRQRVAA 94
TL S SSS+ P L I + TTT+T+TPT SA+ +++P+
Sbjct: 384 TLPPPTSSSASSSTLP-------LRSIPFPTPTTTSTSTPT---SSAAPNTSPRSSPTGG 235
Query: 95 GKISKRKSRASKRSQTTFITADPAN 119
S+R SR + T + A+
Sbjct: 234 SATSRRSSRPPPPATATATSTSSAS 160
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.311 0.125 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,994,995
Number of Sequences: 63676
Number of extensions: 129019
Number of successful extensions: 1329
Number of sequences better than 10.0: 96
Number of HSP's better than 10.0 without gapping: 1131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1246
length of query: 235
length of database: 12,639,632
effective HSP length: 94
effective length of query: 141
effective length of database: 6,654,088
effective search space: 938226408
effective search space used: 938226408
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC149050.15