Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC148996.5 + phase: 0 
         (196 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC227580 similar to PIR|S34045|S34045 protamine - North American...   226  5e-60
TC229433 similar to UP|SSB_ARATH (Q84J78) Single-stranded DNA-bi...    95  2e-20
TC229434 similar to UP|SSB_ARATH (Q84J78) Single-stranded DNA-bi...    89  1e-18
BI969709                                                               37  0.006
TC226606 similar to UP|PIN1_MALDO (Q94G00) Peptidyl-prolyl cis-t...    35  0.029
TC230242 weakly similar to UP|Q9FQZ6 (Q9FQZ6) Avr9/Cf-9 rapidly ...    30  0.55
TC226607 similar to UP|PIN1_MALDO (Q94G00) Peptidyl-prolyl cis-t...    30  0.71
TC205128 similar to UP|Q9SMA5 (Q9SMA5) Zwh0004.1, partial (8%)         29  1.2
TC210418 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (36%)    29  1.6
AW567640 similar to SP|Q9C7E8|COL3_ Zinc finger protein constans...    29  1.6
TC212794 weakly similar to GB|AAQ55274.1|33942043|BT010323 At1g2...    29  1.6
BG839154                                                               28  2.1
TC208968 similar to UP|GBF4_ARATH (P42777) G-box binding factor ...    28  2.1
TC226922 similar to UP|Q9LPQ3 (Q9LPQ3) F15H18.14, partial (54%)        28  2.1
BI469435                                                               28  2.1
TC209317 similar to UP|Q944G2 (Q944G2) Mevalonate kinase, partia...    28  2.7
TC211905 homologue to UP|Q77IW3 (Q77IW3) ORF91, partial (11%)          28  2.7
TC220323 similar to UP|LBD1_ARATH (Q9LQR0) LOB domain protein 1,...    28  2.7
BE822954 similar to GP|16417946|gb mevalonate kinase {Hevea bras...    28  2.7
TC227464 similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partia...    28  3.5

>TC227580 similar to PIR|S34045|S34045 protamine - North American opossum
           {Didelphis virginiana;} , partial (31%)
          Length = 930

 Score =  226 bits (576), Expect = 5e-60
 Identities = 116/193 (60%), Positives = 145/193 (75%), Gaps = 3/193 (1%)
 Frame = +3

Query: 1   MAKFSISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPE--- 57
           M+   ++ +  LY SLL TP    + +RH       +T  L D D+ADSA   P+P+   
Sbjct: 30  MSSSMMTLSRRLYCSLLRTP----NAYRHF------STDHLFDSDEADSAPDPPAPDAVQ 179

Query: 58  QTERTFFDRPLENGLDPGVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPL 117
           + E+   DRPLENGLD G+YRAILVGKAGQ PLQKKLKSGT VTLLS+GTGGI NNRRP 
Sbjct: 180 EEEKFVIDRPLENGLDVGIYRAILVGKAGQAPLQKKLKSGTSVTLLSVGTGGIHNNRRPR 359

Query: 118 DHENPREYANRCAVQWHRVTVYPERLGNLLMKTVLPGSTLYVEGNLETKVFSDPVTGLVR 177
             ENP++YANR A+QWHRV+VYP++LG+L+ K V+PGS LYVEGNLETK F+DP+TG+ R
Sbjct: 360 VDENPKDYANRSAIQWHRVSVYPQKLGDLVTKHVVPGSMLYVEGNLETKCFTDPITGIAR 539

Query: 178 RVREVAVRRNGNL 190
           R+RE+AVRRNG +
Sbjct: 540 RIREIAVRRNGRI 578


>TC229433 similar to UP|SSB_ARATH (Q84J78) Single-stranded DNA-binding
           protein, mitochondrial precursor, partial (73%)
          Length = 846

 Score = 94.7 bits (234), Expect = 2e-20
 Identities = 46/127 (36%), Positives = 80/127 (62%), Gaps = 7/127 (5%)
 Frame = +1

Query: 71  GLDP-------GVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHENPR 123
           G+DP       GV++AI+ GK GQ P+QK L++G  +T+ ++GTGG+ + R     + P+
Sbjct: 229 GVDPKKGWGFRGVHKAIICGKVGQAPVQKILRNGRNITIFTVGTGGMFDQRIQGPKDLPK 408

Query: 124 EYANRCAVQWHRVTVYPERLGNLLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRVREVA 183
                   QWHR+ V+ + LG   ++ +   S++YVEG++ET+V++D + G V+ + E+ 
Sbjct: 409 P------AQWHRIAVHNDILGTYAVQQLFKNSSVYVEGDIETRVYNDSINGNVKSIPEIC 570

Query: 184 VRRNGNL 190
           VRR+G +
Sbjct: 571 VRRDGRI 591


>TC229434 similar to UP|SSB_ARATH (Q84J78) Single-stranded DNA-binding
           protein, mitochondrial precursor, partial (75%)
          Length = 761

 Score = 89.0 bits (219), Expect = 1e-18
 Identities = 44/127 (34%), Positives = 78/127 (60%), Gaps = 7/127 (5%)
 Frame = +2

Query: 71  GLDP-------GVYRAILVGKAGQKPLQKKLKSGTVVTLLSIGTGGIRNNRRPLDHENPR 123
           G+DP       GV++AI+ GK GQ P+QK L++G  + + ++GTGG+ + R     + P+
Sbjct: 65  GVDPKKGWGFRGVHKAIICGKVGQAPVQKILRNGRNLXIFTVGTGGMFDQRIQGPKDLPK 244

Query: 124 EYANRCAVQWHRVTVYPERLGNLLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRVREVA 183
                   QWHR+ V+ + LG   ++ +   S++YVEG++E +V++D + G V+ + E+ 
Sbjct: 245 P------AQWHRIAVHNDILGAYAVQKLFKNSSVYVEGDIEIRVYNDSINGEVKSIPEIC 406

Query: 184 VRRNGNL 190
           VRR+G +
Sbjct: 407 VRRDGKI 427


>BI969709 
          Length = 572

 Score = 37.0 bits (84), Expect = 0.006
 Identities = 24/76 (31%), Positives = 35/76 (45%)
 Frame = -3

Query: 120 ENPREYANRCAVQWHRVTVYPERLGNLLMKTVLPGSTLYVEGNLETKVFSDPVTGLVRRV 179
           + PR + N    Q  R+  + + LG   +        +YVEG  E +VF D  TG V+  
Sbjct: 468 QGPRVFPN--LAQGLRIPGHNDILGPYPVHHFFKTFPVYVEGAFELRVFIDAFTGTVKTF 295

Query: 180 REVAVRRNGNLANSSN 195
            ++ V RNG    S N
Sbjct: 294 PKICVPRNGRFPFSKN 247


>TC226606 similar to UP|PIN1_MALDO (Q94G00) Peptidyl-prolyl cis-trans
           isomerase 1  (Rotamase Pin1) (PPIase Pin1) (MdPin1) ,
           partial (91%)
          Length = 748

 Score = 34.7 bits (78), Expect = 0.029
 Identities = 25/66 (37%), Positives = 32/66 (47%)
 Frame = -1

Query: 7   SRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFFDR 66
           SRT  LY+ + S  P P HHH+       TTTTS +      S +PSP P  T  +  D 
Sbjct: 196 SRTFVLYQYVRS--P*PHHHHQSST----TTTTSSTS*SYCGSPSPSPPPRSTPSSASDL 35

Query: 67  PLENGL 72
            L + L
Sbjct: 34  TLSSPL 17


>TC230242 weakly similar to UP|Q9FQZ6 (Q9FQZ6) Avr9/Cf-9 rapidly elicited
           protein 146, partial (30%)
          Length = 713

 Score = 30.4 bits (67), Expect = 0.55
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +1

Query: 19  TPPPPLHHHRHIP 31
           +PPPPLHHH  +P
Sbjct: 355 SPPPPLHHHHQVP 393



 Score = 28.1 bits (61), Expect = 2.7
 Identities = 14/47 (29%), Positives = 21/47 (43%)
 Frame = +2

Query: 3   KFSISRTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADS 49
           +FS S +  L+  +  +     HHH       CTTTT    +DD  +
Sbjct: 290 EFSCSNSPALFHVIKRSTNKHRHHHHR-----CTTTTKFPHHDDVST 415


>TC226607 similar to UP|PIN1_MALDO (Q94G00) Peptidyl-prolyl cis-trans
           isomerase 1  (Rotamase Pin1) (PPIase Pin1) (MdPin1) ,
           partial (93%)
          Length = 736

 Score = 30.0 bits (66), Expect = 0.71
 Identities = 21/58 (36%), Positives = 27/58 (46%)
 Frame = -3

Query: 15  SLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFFDRPLENGL 72
           S+ + P P   HH        TTTTS + Y  A S +PSP P  T  +  D  L + L
Sbjct: 209 SICAKP*PQYRHHPST-----TTTTSSTSY--AYSGSPSPPPRSTPSSASDLTLSSPL 57


>TC205128 similar to UP|Q9SMA5 (Q9SMA5) Zwh0004.1, partial (8%)
          Length = 411

 Score = 29.3 bits (64), Expect = 1.2
 Identities = 9/10 (90%), Positives = 9/10 (90%)
 Frame = +2

Query: 20  PPPPLHHHRH 29
           PPPPLHHH H
Sbjct: 113 PPPPLHHHHH 142


>TC210418 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (36%)
          Length = 598

 Score = 28.9 bits (63), Expect = 1.6
 Identities = 13/22 (59%), Positives = 14/22 (63%)
 Frame = +2

Query: 8  RTSTLYRSLLSTPPPPLHHHRH 29
          +TST   SL   PPPPL H RH
Sbjct: 26 QTSTTSVSLSPMPPPPLSHARH 91


>AW567640 similar to SP|Q9C7E8|COL3_ Zinc finger protein constans-like 3.
           [Mouse-ear cress] {Arabidopsis thaliana}, partial (11%)
          Length = 386

 Score = 28.9 bits (63), Expect = 1.6
 Identities = 18/58 (31%), Positives = 21/58 (36%), Gaps = 13/58 (22%)
 Frame = +2

Query: 23  PLHHHRHIPLRFCTTTTSLSDYDDAD-------------SAAPSPSPEQTERTFFDRP 67
           PL HH H P    TT T    Y  A+                P+PSP  T  + F  P
Sbjct: 125 PLTHHHHSPCCHATTATPNPPYSSAELIPLNSV*FATSTCTPPTPSPSSTCASRFATP 298


>TC212794 weakly similar to GB|AAQ55274.1|33942043|BT010323 At1g23980
           {Arabidopsis thaliana;} , partial (7%)
          Length = 625

 Score = 28.9 bits (63), Expect = 1.6
 Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
 Frame = -2

Query: 8   RTSTLYRSLLSTPPP----PLHHHRHIPLRFCTTTTSLSDYDDADSAAP 52
           R+S L+ S LS+ PP    P HHH H+ +    T  + S+++      P
Sbjct: 192 RSSHLWLSPLSSSPPHSHFPKHHHHHLEIENEITRLANSNHEPHTDTNP 46


>BG839154 
          Length = 787

 Score = 28.5 bits (62), Expect = 2.1
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = -2

Query: 9   TSTLYRSLLSTPPPPLHHHR 28
           TS LY  L S+PPP  HH R
Sbjct: 597 TSHLYHYLSSSPPPHFHHSR 538


>TC208968 similar to UP|GBF4_ARATH (P42777) G-box binding factor 4
           (AtbZIP40), partial (27%)
          Length = 1199

 Score = 28.5 bits (62), Expect = 2.1
 Identities = 14/50 (28%), Positives = 27/50 (54%)
 Frame = +2

Query: 20  PPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERTFFDRPLE 69
           PPPP      +P     +++S+  + +  SAAPS S ++ +R   + P++
Sbjct: 377 PPPPPPPSSFLPFPADGSSSSVEPFANGVSAAPSNSVQKGKRRAVEEPVD 526


>TC226922 similar to UP|Q9LPQ3 (Q9LPQ3) F15H18.14, partial (54%)
          Length = 1190

 Score = 28.5 bits (62), Expect = 2.1
 Identities = 10/14 (71%), Positives = 10/14 (71%)
 Frame = +3

Query: 20  PPPPLHHHRHIPLR 33
           PPPP HHHR   LR
Sbjct: 153 PPPPSHHHRQTSLR 194


>BI469435 
          Length = 426

 Score = 28.5 bits (62), Expect = 2.1
 Identities = 20/53 (37%), Positives = 24/53 (44%)
 Frame = +3

Query: 10 STLYRSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQTERT 62
          S+    LLS       HH+  P    TT+ S S    A SA P PSP+ T  T
Sbjct: 6  SSTLTPLLSPHASSSQHHKP-PAAATTTSLS*STSTAAPSAPPPPSPQTTTTT 161


>TC209317 similar to UP|Q944G2 (Q944G2) Mevalonate kinase, partial (38%)
          Length = 534

 Score = 28.1 bits (61), Expect = 2.7
 Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
 Frame = -2

Query: 16  LLSTPPPPL----HHHRHIPLRFCTTTTSLSDYDDADSAAPSPSP 56
           L+STPPPP+    H + H      T +T++ D +   S    P P
Sbjct: 425 LISTPPPPIPAMKHWNPHDSSSATTLSTTVPDSNVGSSVRTQPPP 291


>TC211905 homologue to UP|Q77IW3 (Q77IW3) ORF91, partial (11%)
          Length = 639

 Score = 28.1 bits (61), Expect = 2.7
 Identities = 14/33 (42%), Positives = 16/33 (48%)
 Frame = +2

Query: 8  RTSTLYRSLLSTPPPPLHHHRHIPLRFCTTTTS 40
          RT  L   LL  PPPPL    H+ L  C   T+
Sbjct: 2  RTLILLSLLLPPPPPPLAFSHHLSLLCCVV*TA 100


>TC220323 similar to UP|LBD1_ARATH (Q9LQR0) LOB domain protein 1, partial
           (69%)
          Length = 854

 Score = 28.1 bits (61), Expect = 2.7
 Identities = 15/45 (33%), Positives = 21/45 (46%)
 Frame = +1

Query: 14  RSLLSTPPPPLHHHRHIPLRFCTTTTSLSDYDDADSAAPSPSPEQ 58
           ++  STP PP+  H  +P     +  S S    + S  P PSP Q
Sbjct: 88  KTTTSTPSPPIPIH--LPTMLSNSPPSSSSSPPSQSPTPFPSPSQ 216


>BE822954 similar to GP|16417946|gb mevalonate kinase {Hevea brasiliensis},
           partial (34%)
          Length = 481

 Score = 28.1 bits (61), Expect = 2.7
 Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
 Frame = +2

Query: 16  LLSTPPPPL----HHHRHIPLRFCTTTTSLSDYDDADSAAPSPSP 56
           L+STPPPP+    H + H      T +T++ D +   S    P P
Sbjct: 92  LISTPPPPIPAMKHWNPHDSSSATTLSTTVPDSNVGSSVRTQPPP 226


>TC227464 similar to UP|Q8VYI9 (Q8VYI9) AT5g64080/MHJ24_6, partial (53%)
          Length = 829

 Score = 27.7 bits (60), Expect = 3.5
 Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 11/58 (18%)
 Frame = +3

Query: 22  PPLHHHRHIPLRF----CTTTTSLSDYDDADSAAPSPSPEQTER-------TFFDRPL 68
           P  HHH H PL       T  T  S +  A  ++P+ +P Q++R       + F +PL
Sbjct: 72  PREHHHHHHPLTRQRPPWTAPTWFSPWPIACPSSPTAAPSQSQRAHAALV*SLFSKPL 245


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.317    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,275,286
Number of Sequences: 63676
Number of extensions: 157604
Number of successful extensions: 2027
Number of sequences better than 10.0: 73
Number of HSP's better than 10.0 without gapping: 1829
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1978
length of query: 196
length of database: 12,639,632
effective HSP length: 92
effective length of query: 104
effective length of database: 6,781,440
effective search space: 705269760
effective search space used: 705269760
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)


Medicago: description of AC148996.5