BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.16 - phase: 0 /pseudo
(332 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220706 191 4e-69
BE556599 similar to GP|22831203|dbj P0496C02.23 {Oryza sativa (j... 49 3e-06
TC228794 similar to UP|Q6V8V0 (Q6V8V0) Transducin-like (Fragment... 45 3e-05
TC208265 similar to UP|Q6S7B0 (Q6S7B0) TAF5, partial (36%) 36 0.021
TC231770 similar to UP|Q8L4J2 (Q8L4J2) Cleavage stimulation fact... 35 0.036
TC222756 similar to UP|Q9LV35 (Q9LV35) WD40-repeat protein, part... 34 0.11
TC206061 weakly similar to UP|Q9FNN2 (Q9FNN2) WD-repeat protein-... 32 0.52
TC207354 weakly similar to UP|WDR5_HUMAN (P61964) WD-repeat prot... 31 0.68
TC220562 similar to UP|Q9LV35 (Q9LV35) WD40-repeat protein, part... 31 0.68
TC227586 UP|Q9ZS21 (Q9ZS21) Glyoxalase I , complete 30 1.2
TC229097 similar to PIR|T46032|T46032 WD-40 repeat regulatory pr... 30 1.5
TC208554 UP|Q9FUF3 (Q9FUF3) FAS2, complete 30 1.5
TC226136 similar to UP|O48847 (O48847) Expressed protein (At2g32... 30 1.5
TC217730 similar to GB|AAQ56808.1|34098851|BT010365 At5g07720 {A... 30 2.0
TC228843 weakly similar to UP|BIN4_HUMAN (O15213) WD-repeat prot... 30 2.0
TC203361 similar to UP|Q6VBJ4 (Q6VBJ4) Epa5p, partial (7%) 30 2.0
TC229333 similar to UP|Q6H976 (Q6H976) STYLOSA protein, partial ... 29 2.6
TC207353 weakly similar to UP|WDR5_HUMAN (P61964) WD-repeat prot... 29 2.6
AW761058 29 2.6
TC206060 weakly similar to UP|Q9FNN2 (Q9FNN2) WD-repeat protein-... 29 2.6
>TC220706
Length = 891
Score = 191 bits (485), Expect(2) = 4e-69
Identities = 96/122 (78%), Positives = 107/122 (87%)
Frame = +2
Query: 211 RIITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 270
RIITGSENG+INLVGILPNR+I+PIAEHSEYPVE LAFSHD+KFLGSI HDQMLKLWDLD
Sbjct: 329 RIITGSENGIINLVGILPNRVIQPIAEHSEYPVECLAFSHDKKFLGSIAHDQMLKLWDLD 508
Query: 271 NILQGSRSTQRNETGVVANDGDSDDGDEMDVDNSASKFSKGNKRKNASNGHAVGDSNNFF 330
NILQGS +TQRNE G DSDD DEMD+D+ SK +KGNKRKNA+NG+A+G SNNFF
Sbjct: 509 NILQGSGNTQRNEAGGAV---DSDD-DEMDLDDDPSKINKGNKRKNANNGNALGGSNNFF 676
Query: 331 AD 332
AD
Sbjct: 677 AD 682
Score = 88.6 bits (218), Expect(2) = 4e-69
Identities = 44/58 (75%), Positives = 48/58 (81%)
Frame = +3
Query: 156 NFLKMSCCLWF**RMAGKLFADHKLESCYCIHGDASRIAVIDSLILLQTLLIPC*RII 213
NFLKMS CLWF**RM GKL+ADHKLES YCIHGDASRI ID LI L TLL+ C* ++
Sbjct: 150 NFLKMSYCLWF**RMVGKLYADHKLESFYCIHGDASRIVAIDLLISLLTLLMLC*SLM 323
Score = 72.8 bits (177), Expect = 2e-13
Identities = 34/41 (82%), Positives = 35/41 (84%)
Frame = -2
Query: 156 NFLKMSCCLWF**RMAGKLFADHKLESCYCIHGDASRIAVI 196
NFLKMS CLWF**RM GKL+ADH LES YCIHGDASRI I
Sbjct: 125 NFLKMSYCLWF**RMVGKLYADHXLESFYCIHGDASRIVAI 3
>BE556599 similar to GP|22831203|dbj P0496C02.23 {Oryza sativa (japonica
cultivar-group)}, partial (7%)
Length = 167
Score = 48.9 bits (115), Expect = 3e-06
Identities = 22/24 (91%), Positives = 24/24 (99%)
Frame = +2
Query: 98 AAVNRLINLTESTVASGDDDGCIK 121
AA+NRLINLTESTVASGDD+GCIK
Sbjct: 77 AAINRLINLTESTVASGDDEGCIK 148
>TC228794 similar to UP|Q6V8V0 (Q6V8V0) Transducin-like (Fragment), complete
Length = 870
Score = 45.4 bits (106), Expect = 3e-05
Identities = 32/114 (28%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
Frame = +3
Query: 10 FDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAV 69
+ + F+P+ ++VASG D ++ L+ N + D + + H + + G +
Sbjct: 291 YTMKFNPAGSVVASGSHDREIFLW-----NVHGDCKNFMVLKGHKNAVLDLHWTTDGTQI 455
Query: 70 LTGSPDFSILATDVETGSTIARLDNAHEAAVNRLINLTES--TVASGDDDGCIK 121
++ SPD ++ A DVETG I ++ H + VN V SG DDG K
Sbjct: 456 VSASPDKTVRAWDVETGKQIKKMVE-HLSYVNSCCPSRRGPPLVVSGSDDGTAK 614
>TC208265 similar to UP|Q6S7B0 (Q6S7B0) TAF5, partial (36%)
Length = 1048
Score = 36.2 bits (82), Expect = 0.021
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 1/95 (1%)
Frame = +2
Query: 188 GDASRIAVIDSLILLQTLLIPC*RII-TGSENGMINLVGILPNRIIEPIAEHSEYPVERL 246
G+ R+ V ++L + P R + +G E+G I + + R + P+ H+ V L
Sbjct: 335 GECVRVFVGHRGMILSLAMSPDGRYMASGDEDGTIMMWDLSSGRCLTPLIGHTSC-VWSL 511
Query: 247 AFSHDRKFLGSIGHDQMLKLWDLDNILQGSRSTQR 281
AFS + + S D +KLWD++ + SR+ ++
Sbjct: 512 AFSSEGSVIASGSADCTVKLWDVNTSTKVSRAEEK 616
Score = 28.1 bits (61), Expect = 5.8
Identities = 17/61 (27%), Positives = 28/61 (45%)
Frame = +2
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDN 271
I++ S + I L N + H+ YPV + FS + S HD+ ++W +D
Sbjct: 32 ILSSSADSTIRLWSTKLNANLVCYKGHN-YPVWDVQFSPVGHYFASSSHDRTARIWSMDR 208
Query: 272 I 272
I
Sbjct: 209 I 211
>TC231770 similar to UP|Q8L4J2 (Q8L4J2) Cleavage stimulation factor 50K chain
(Cleavage stimulation factor 50), partial (38%)
Length = 753
Score = 35.4 bits (80), Expect = 0.036
Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +3
Query: 213 ITGSENGMINLV-GILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDL 269
+T S++G I L GI N + A H F+ D++F+ S G D LKLW++
Sbjct: 45 VTASKDGAIRLWDGITANCVRSITAAHGTAEATSAIFTKDQRFVLSCGKDSTLKLWEV 218
>TC222756 similar to UP|Q9LV35 (Q9LV35) WD40-repeat protein, partial (28%)
Length = 591
Score = 33.9 bits (76), Expect = 0.11
Identities = 39/129 (30%), Positives = 53/129 (40%), Gaps = 11/129 (8%)
Frame = +1
Query: 3 INLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARF 62
INLG + P N G DG LH+Y S D + VLE H
Sbjct: 196 INLGLIVTASAVSPDGNEAIIGGQDGKLHIYSISGDTLVEE--AVLEKH----------- 336
Query: 63 INGGRAVLTGSPDFSILAT-DV-------ETGSTIARLDNA--HEAAVNRLINLTES-TV 111
G +V+ SPD S+ A+ DV + S +L N H A +N L +S +
Sbjct: 337 -RGAISVIRYSPDLSMFASGDVNREAIVWDRASREVKLKNMLYHTARINCLAWSPDSLRI 513
Query: 112 ASGDDDGCI 120
A+G D C+
Sbjct: 514 ATGSLDTCV 540
>TC206061 weakly similar to UP|Q9FNN2 (Q9FNN2) WD-repeat protein-like,
partial (36%)
Length = 657
Score = 31.6 bits (70), Expect = 0.52
Identities = 16/59 (27%), Positives = 33/59 (55%)
Frame = +3
Query: 227 LPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDNILQGSRSTQRNETG 285
+P+R ++ + H + V + FSH+ K+L S +D+ +W++D + G S + +G
Sbjct: 174 IPSRTLQILEAHDD-EVWYVQFSHNGKYLASASNDRSAIIWEVD--MNGELSVKHKLSG 341
>TC207354 weakly similar to UP|WDR5_HUMAN (P61964) WD-repeat protein 5,
partial (57%)
Length = 1124
Score = 31.2 bits (69), Expect = 0.68
Identities = 20/92 (21%), Positives = 38/92 (40%)
Frame = +1
Query: 10 FDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAV 69
F ++F+P + + SG D + ++ + V + HT + + G +
Sbjct: 403 FCVNFNPQSSYIVSGSFDETIKVWDVKTGKC------VHTIKGHTMPVTSVHYNRDGNLI 564
Query: 70 LTGSPDFSILATDVETGSTIARLDNAHEAAVN 101
++ S D S D ETG+ + L AV+
Sbjct: 565 ISASHDGSCKIWDTETGNLLKTLIEDKAPAVS 660
Score = 30.0 bits (66), Expect = 1.5
Identities = 15/59 (25%), Positives = 29/59 (48%)
Frame = +1
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLD 270
I++GS + I + + + + I H+ PV + ++ D + S HD K+WD +
Sbjct: 436 IVSGSFDETIKVWDVKTGKCVHTIKGHT-MPVTSVHYNRDGNLIISASHDGSCKIWDTE 609
>TC220562 similar to UP|Q9LV35 (Q9LV35) WD40-repeat protein, partial (29%)
Length = 867
Score = 31.2 bits (69), Expect = 0.68
Identities = 38/129 (29%), Positives = 53/129 (40%), Gaps = 11/129 (8%)
Frame = +2
Query: 3 INLGKLAFDIDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARF 62
INL + P + G DG LH+Y S D VLE H
Sbjct: 116 INLCFIVTAAAMSPDGSEAIVGGQDGKLHMYSVSGDTVTEQ--AVLEKH----------- 256
Query: 63 INGGRAVLTGSPDFSILAT-DV-------ETGSTIARLDNA--HEAAVNRLINLTEST-V 111
G V+ SPDFS+ A+ D+ + S +L+N + A +N L +ST V
Sbjct: 257 -RGAITVIRYSPDFSMFASADLNREAVVWDRASKEVKLNNMLFNTARINCLAWSPDSTLV 433
Query: 112 ASGDDDGCI 120
A+G D C+
Sbjct: 434 ATGSLDTCV 460
Score = 29.6 bits (65), Expect = 2.0
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 69 VLTGSPDFSILATDV-ETGSTIARLDNAHEAAVNRLINLTESTVASGDDDGCIK 121
V TGS D ++ +V + S+ + AH V L+ + + V S +DGC++
Sbjct: 431 VATGSLDTCVIIYEVGKPASSRRTIKGAHIGGVYGLVFIDQERVVSSGEDGCVR 592
>TC227586 UP|Q9ZS21 (Q9ZS21) Glyoxalase I , complete
Length = 861
Score = 30.4 bits (67), Expect = 1.2
Identities = 18/41 (43%), Positives = 19/41 (45%), Gaps = 1/41 (2%)
Frame = -2
Query: 148 EIKSKPNLNFLKMSC-CLWF**RMAGKLFADHKLESCYCIH 187
EIK K N F SC WF KLF KLE Y +H
Sbjct: 833 EIKEKKNTRFTTQSCFSFWF----DNKLFIQVKLEKSYILH 723
>TC229097 similar to PIR|T46032|T46032 WD-40 repeat regulatory protein tup1
homolog - Arabidopsis thaliana {Arabidopsis thaliana;} ,
partial (47%)
Length = 755
Score = 30.0 bits (66), Expect = 1.5
Identities = 17/63 (26%), Positives = 32/63 (49%), Gaps = 7/63 (11%)
Frame = +1
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSE-------YPVERLAFSHDRKFLGSIGHDQML 264
I+ GSE+ I L + +I++ + HS+ +P E + S G++G+D +
Sbjct: 307 IVGGSEDNCIYLWDLQSRKIVQKLEGHSDAVVSVSCHPTENMIAS------GALGNDNTV 468
Query: 265 KLW 267
K+W
Sbjct: 469 KIW 477
>TC208554 UP|Q9FUF3 (Q9FUF3) FAS2, complete
Length = 1945
Score = 30.0 bits (66), Expect = 1.5
Identities = 26/123 (21%), Positives = 53/123 (42%), Gaps = 9/123 (7%)
Frame = +1
Query: 6 GKLAFDIDFHPSENLVASGLIDGDLHLYRYS-SDNTNSDPV--RVLEVHAHTESCRAARF 62
GK +DFHP +A+ D D+ ++ + + PV + + H+ + RF
Sbjct: 37 GKPVLTLDFHPHSATLATAGADFDIKFWQIKPAGSPKKLPVVSYLSNLSYHSSAVNVIRF 216
Query: 63 INGGRAVLTGSPDFSIL-----ATDVETGSTIARLDNAHEAAVNRLINLTEST-VASGDD 116
+ G + +G+ ++ +TD + ++ +H + L T++T + SG
Sbjct: 217 SSSGEXLASGADGGDLIIWKLHSTDAGQTWKVLKMLRSHHKDILDLQWSTDATYIISGSV 396
Query: 117 DGC 119
D C
Sbjct: 397 DNC 405
Score = 29.6 bits (65), Expect = 2.0
Identities = 21/86 (24%), Positives = 39/86 (44%), Gaps = 1/86 (1%)
Frame = +1
Query: 12 IDFHPSENLVASGLIDGDLHLYRYSSDNTNSDPVRVLEVHAHTESCRAARFINGGRAVLT 71
I F S +ASG GDL +++ S + + + +H + ++ +++
Sbjct: 208 IRFSSSGEXLASGADGGDLIIWKLHSTDAGQTWKVLKMLRSHHKDILDLQWSTDATYIIS 387
Query: 72 GSPDFSILATDVETGSTIARLD-NAH 96
GS D + DV G+ + LD +AH
Sbjct: 388 GSVDNCCIIWDVNKGTNLQTLDTHAH 465
>TC226136 similar to UP|O48847 (O48847) Expressed protein
(At2g32700/F24L7.16), partial (42%)
Length = 1463
Score = 30.0 bits (66), Expect = 1.5
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = +3
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNI 272
FS D K L S GHD+ + LW+++ +
Sbjct: 255 FSSDGKLLASAGHDKKVVLWNMETL 329
>TC217730 similar to GB|AAQ56808.1|34098851|BT010365 At5g07720 {Arabidopsis
thaliana;} , partial (46%)
Length = 950
Score = 29.6 bits (65), Expect = 2.0
Identities = 23/77 (29%), Positives = 35/77 (44%), Gaps = 2/77 (2%)
Frame = +3
Query: 233 EPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDLDN--ILQGSRSTQRNETGVVAND 290
+P + +YPVER S +R F + +Q+LKL+ + +L RNET
Sbjct: 432 KPCGSYGDYPVERCLSSMERAF--NFADNQVLKLYGFRHRGLLSPKIKRIRNETVSPLEF 605
Query: 291 GDSDDGDEMDVDNSASK 307
D D +N+ SK
Sbjct: 606 VDQFDIRRHSTENTESK 656
>TC228843 weakly similar to UP|BIN4_HUMAN (O15213) WD-repeat protein BING4,
partial (5%)
Length = 1377
Score = 29.6 bits (65), Expect = 2.0
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +1
Query: 231 IIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDL 269
+++ + H PV LAF + + + G D+ +KLWDL
Sbjct: 364 LVKMLCHHG--PVSALAFHSNGLLMATAGKDKKIKLWDL 474
>TC203361 similar to UP|Q6VBJ4 (Q6VBJ4) Epa5p, partial (7%)
Length = 1090
Score = 29.6 bits (65), Expect = 2.0
Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
Frame = -1
Query: 274 QGSRSTQRNET--GVVANDGDSDDGDEMDVDNSASKFSKGNKRKNASNGHAVGDSNN 328
+G R TQ + DG+ D+GD D D+ F +G + ++ +G G+++N
Sbjct: 643 KGERKTQSQDKQRDAEGEDGNDDEGD--DDDDGDGGFGEGEEELSSEDGGGYGNNSN 479
>TC229333 similar to UP|Q6H976 (Q6H976) STYLOSA protein, partial (32%)
Length = 982
Score = 29.3 bits (64), Expect = 2.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNILQ 274
FS D K L S GHD+ + LW D++ Q
Sbjct: 684 FSSDGKLLASGGHDKRVVLWYTDSLKQ 764
>TC207353 weakly similar to UP|WDR5_HUMAN (P61964) WD-repeat protein 5,
partial (58%)
Length = 898
Score = 29.3 bits (64), Expect = 2.6
Identities = 15/57 (26%), Positives = 28/57 (48%)
Frame = +3
Query: 212 IITGSENGMINLVGILPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWD 268
I++GS + I + + + + I H+ PV + ++ D + S HD K+WD
Sbjct: 39 IVSGSFDETIKVWDVKTGKCVHTIKGHT-MPVTSVHYNRDGTLIISASHDGSCKIWD 206
>AW761058
Length = 355
Score = 29.3 bits (64), Expect = 2.6
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3
Query: 248 FSHDRKFLGSIGHDQMLKLWDLDNILQ 274
FS D K L S GHD+ + LW D++ Q
Sbjct: 213 FSSDGKLLASGGHDKRVVLWYTDSLKQ 293
>TC206060 weakly similar to UP|Q9FNN2 (Q9FNN2) WD-repeat protein-like,
partial (62%)
Length = 1510
Score = 29.3 bits (64), Expect = 2.6
Identities = 13/43 (30%), Positives = 25/43 (57%)
Frame = +3
Query: 227 LPNRIIEPIAEHSEYPVERLAFSHDRKFLGSIGHDQMLKLWDL 269
+P+R ++ + H + V + FSH+ K+L S DQ +W++
Sbjct: 387 IPSRTLQILEAHDD-EVWYVQFSHNGKYLASASKDQTAIIWEV 512
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.141 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,024,552
Number of Sequences: 63676
Number of extensions: 160720
Number of successful extensions: 1038
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 1010
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1034
length of query: 332
length of database: 12,639,632
effective HSP length: 98
effective length of query: 234
effective length of database: 6,399,384
effective search space: 1497455856
effective search space used: 1497455856
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148996.16