BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148996.15 + phase: 0
(153 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC219290 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), pa... 150 3e-37
TC221966 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), pa... 115 8e-27
BG509584 similar to GP|7770334|gb|A F27J15.21 {Arabidopsis thali... 68 2e-12
TC212945 weakly similar to UP|Q9M9H2 (Q9M9H2) F14O23.12, partial... 47 5e-06
CA850743 39 0.001
TC210888 similar to UP|Q41051 (Q41051) Proline- and leucine-rich... 37 0.003
CA802337 33 0.070
AW755554 32 0.16
TC215705 homologue to PIR|G96755|G96755 developmental protein ho... 31 0.20
TC217096 homologue to UP|Q8W1S2 (Q8W1S2) ABC transporter-like pr... 31 0.26
TC222474 similar to UP|Q9LJU8 (Q9LJU8) Arabidopsis thaliana geno... 30 0.35
TC215703 homologue to PIR|G96755|G96755 developmental protein ho... 30 0.45
TC226172 similar to UP|Q8VZI2 (Q8VZI2) AT4g33700/T16L1_190, part... 29 0.77
TC228903 29 1.0
TC230207 similar to UP|Q9LTR6 (Q9LTR6) Gb|AAF36750.1, partial (28%) 28 2.2
TC225721 homologue to UP|Q7S1C8 (Q7S1C8) Predicted protein, part... 28 2.2
TC223468 similar to GB|AAS92337.1|46402470|BT012421 At5g24330 {A... 28 2.2
TC225719 28 2.2
BG653461 27 2.9
TC207509 27 2.9
>TC219290 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), partial (32%)
Length = 685
Score = 150 bits (378), Expect = 3e-37
Identities = 84/152 (55%), Positives = 102/152 (66%), Gaps = 8/152 (5%)
Frame = +2
Query: 9 SPNRHHSNGGFFVTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMA 68
SP RH+ NG F VTISAILALL ++ + K +++E + +
Sbjct: 86 SPKRHNHNG-FIVTISAILALLKKR----------KAAVKASKEDELK---------KSS 205
Query: 69 KPKKLLSNISNKALSQFGKKKQR--------EEREKEGWGNGGVWQKEILMGGKCEPLDF 120
+PKKLLSNIS+KAL FG KK E ++ +GWGNGGVWQKEILMGGKCEPL+F
Sbjct: 206 RPKKLLSNISSKAL--FGHKKNNKKQLRSVEETQQAQGWGNGGVWQKEILMGGKCEPLNF 379
Query: 121 SGVIYYDINGKQTREVPIRSPRASPLPGYLTR 152
SGVIYYD NGKQ ++P+RSPRASPLPGYLTR
Sbjct: 380 SGVIYYDGNGKQLSQIPLRSPRASPLPGYLTR 475
>TC221966 similar to UP|Q9M9A2 (Q9M9A2) F27J15.21 (At1g49000), partial (29%)
Length = 773
Score = 115 bits (288), Expect = 8e-27
Identities = 60/93 (64%), Positives = 68/93 (72%)
Frame = +3
Query: 55 WRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREEREKEGWGNGGVWQKEILMGGK 114
WR +L++P KKLLSNIS+K L F KKK+ EE WG GGVWQK ILMG K
Sbjct: 81 WRMELRSP--------KKLLSNISSKTLP-FRKKKRGEE----DWGKGGVWQKAILMGDK 221
Query: 115 CEPLDFSGVIYYDINGKQTREVPIRSPRASPLP 147
CEPLDFSGVIYYD NGKQ E+P+RSPRASP+P
Sbjct: 222 CEPLDFSGVIYYDSNGKQVNEMPLRSPRASPVP 320
>BG509584 similar to GP|7770334|gb|A F27J15.21 {Arabidopsis thaliana},
partial (23%)
Length = 327
Score = 67.8 bits (164), Expect = 2e-12
Identities = 29/36 (80%), Positives = 32/36 (88%)
Frame = +2
Query: 112 GGKCEPLDFSGVIYYDINGKQTREVPIRSPRASPLP 147
G KCEPLDFSGVIYYD NGKQ E+P+RSPRASP+P
Sbjct: 2 GDKCEPLDFSGVIYYDSNGKQVNEMPLRSPRASPVP 109
>TC212945 weakly similar to UP|Q9M9H2 (Q9M9H2) F14O23.12, partial (15%)
Length = 625
Score = 46.6 bits (109), Expect = 5e-06
Identities = 29/66 (43%), Positives = 36/66 (53%), Gaps = 6/66 (9%)
Frame = +2
Query: 72 KLLSNISNKALSQFGKKKQREEREKEGWGN------GGVWQKEILMGGKCEPLDFSGVIY 125
KL SNI+N+ALS R+ RE E G+ +W+K ILMG KC PL+FSG
Sbjct: 224 KLNSNINNRALSMVKMISWRKVREGEVEGDYSDQDEEALWRKNILMGEKCRPLNFSGKFE 403
Query: 126 YDINGK 131
D K
Sbjct: 404 NDSEEK 421
>CA850743
Length = 328
Score = 38.5 bits (88), Expect = 0.001
Identities = 15/18 (83%), Positives = 18/18 (99%)
Frame = +3
Query: 135 EVPIRSPRASPLPGYLTR 152
++P+RSPRASPLPGYLTR
Sbjct: 42 QIPLRSPRASPLPGYLTR 95
>TC210888 similar to UP|Q41051 (Q41051) Proline- and leucine-rich protein,
partial (12%)
Length = 771
Score = 37.4 bits (85), Expect = 0.003
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = +3
Query: 104 VWQKEILMGGKCEPLDFSGVIYYDINGKQTRE 135
VWQ+ IL G KCE FSG+I YD G+ R+
Sbjct: 501 VWQRPILRGEKCELPRFSGLILYDEKGRLLRD 596
>CA802337
Length = 381
Score = 32.7 bits (73), Expect = 0.070
Identities = 25/102 (24%), Positives = 44/102 (42%)
Frame = +3
Query: 21 VTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNK 80
+ +++I LL +K + K+K K K K K KK K
Sbjct: 12 IKLNSIK*LLKKKKKKKKKKKKK----------------KKKKKKKKKKKKKKKKKKKKK 143
Query: 81 ALSQFGKKKQREEREKEGWGNGGVWQKEILMGGKCEPLDFSG 122
+ KKK++++++K+ GG +K+ L GG + F+G
Sbjct: 144 KKKKKKKKKKKKKKKKKKKXGGGFKKKKFLXGGNGKKSFFNG 269
Score = 31.6 bits (70), Expect = 0.16
Identities = 23/83 (27%), Positives = 36/83 (42%)
Frame = +1
Query: 38 KEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQREEREKE 97
K+K K K + ++ + K K K KK K + KKK +K+
Sbjct: 46 KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKXGGGLKKK 225
Query: 98 GWGNGGVWQKEILMGGKCEPLDF 120
+ GG+ +K LMGGK + + F
Sbjct: 226 SF*XGGMEKKVFLMGGKKKKIFF 294
>AW755554
Length = 436
Score = 31.6 bits (70), Expect = 0.16
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -3
Query: 69 KPKKLLSNISNKALSQFGKKKQREEREKEGWG 100
K ++ L + N FG K+ ERE+EGWG
Sbjct: 131 K*RRFLESAENDKNGSFGPLKREREREREGWG 36
>TC215705 homologue to PIR|G96755|G96755 developmental protein homolog DG1118
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (57%)
Length = 582
Score = 31.2 bits (69), Expect = 0.20
Identities = 16/45 (35%), Positives = 23/45 (50%)
Frame = +3
Query: 31 SRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLS 75
SR+ R +++SSS T R WR + PP SP+ K + S
Sbjct: 72 SRRCRRPWTRSRSSS*TWRSRPSSWRAPWRDPPPSPLPKERSTAS 206
>TC217096 homologue to UP|Q8W1S2 (Q8W1S2) ABC transporter-like protein,
complete
Length = 2478
Score = 30.8 bits (68), Expect = 0.26
Identities = 20/65 (30%), Positives = 30/65 (45%)
Frame = +3
Query: 33 KANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFGKKKQRE 92
K +KEK S P R E D++ P K P LLS L ++K+++
Sbjct: 354 KHGLVKEKPAVRSLAAPFRMNEGMDDVQAPKKKPEPVDGPLLSERDRLKLE---RRKRKD 524
Query: 93 EREKE 97
ER++E
Sbjct: 525 ERQRE 539
>TC222474 similar to UP|Q9LJU8 (Q9LJU8) Arabidopsis thaliana genomic DNA,
chromosome 3, TAC clone: K10D20, partial (7%)
Length = 781
Score = 30.4 bits (67), Expect = 0.35
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = -1
Query: 91 REEREKEGWGNGGVWQKEI 109
R E+E +GWGNGG +++++
Sbjct: 325 RREKEMDGWGNGGAFRRDL 269
>TC215703 homologue to PIR|G96755|G96755 developmental protein homolog DG1118
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , complete
Length = 896
Score = 30.0 bits (66), Expect = 0.45
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = +3
Query: 31 SRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLS 75
SR+ R +++SSS T R WR PP SP+ K + S
Sbjct: 420 SRRCRRPWTRSRSSS*TWRSRPSSWRAPWPDPPPSPLPKERSTAS 554
>TC226172 similar to UP|Q8VZI2 (Q8VZI2) AT4g33700/T16L1_190, partial (62%)
Length = 1134
Score = 29.3 bits (64), Expect = 0.77
Identities = 16/71 (22%), Positives = 33/71 (45%)
Frame = +3
Query: 27 LALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSNISNKALSQFG 86
+A++ R+ ++ +++ ++ +RD + D + PPK KPK L K+
Sbjct: 468 MAVVVRRCDKTNQQSSQNNANDSVRDVKVDIDGEKPPKEKALKPKMPLHKW--KSFPNTN 641
Query: 87 KKKQREEREKE 97
K R R ++
Sbjct: 642 KSSNRGSRSRK 674
>TC228903
Length = 1479
Score = 28.9 bits (63), Expect = 1.0
Identities = 17/56 (30%), Positives = 31/56 (55%)
Frame = +3
Query: 21 VTISAILALLSRKANRLKEKAKSSSTTKPIRDEEWRFDLKTPPKSPMAKPKKLLSN 76
+T S I+A+ S+ ++ +K + + K +DEE + TP P++K + L SN
Sbjct: 363 ITASWIMAMDSKFSSTIKNNSDNDYNLKKKKDEELSVQISTP---PISKVETLNSN 521
>TC230207 similar to UP|Q9LTR6 (Q9LTR6) Gb|AAF36750.1, partial (28%)
Length = 823
Score = 27.7 bits (60), Expect = 2.2
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +3
Query: 86 GKKKQREEREKEGWGNGGVWQKEILMGG 113
G+ K++ + E+ NGG+W++++ GG
Sbjct: 528 GRMKRKRKPERHAQMNGGMWRQKVSNGG 611
>TC225721 homologue to UP|Q7S1C8 (Q7S1C8) Predicted protein, partial (13%)
Length = 1010
Score = 27.7 bits (60), Expect = 2.2
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +1
Query: 70 PKKLLSNISNKALSQFGKKKQREEREKEGWGNGG 103
P+K + + + + F + E+E+EG+G GG
Sbjct: 604 PRKPATTVEDGVTTSFASCAKEAEKEREGYGGGG 705
>TC223468 similar to GB|AAS92337.1|46402470|BT012421 At5g24330 {Arabidopsis
thaliana;} , partial (33%)
Length = 507
Score = 27.7 bits (60), Expect = 2.2
Identities = 11/29 (37%), Positives = 17/29 (57%)
Frame = +1
Query: 88 KKQREEREKEGWGNGGVWQKEILMGGKCE 116
K + E K GWG GG+ ++E +G + E
Sbjct: 316 KSRHAEEAKAGWGLGGIEEEEEAVGVRAE 402
>TC225719
Length = 1083
Score = 27.7 bits (60), Expect = 2.2
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +1
Query: 70 PKKLLSNISNKALSQFGKKKQREEREKEGWGNGG 103
P+K + + + + F + E+E+EG+G GG
Sbjct: 706 PRKPATTVEDGVTTSFASCAKEAEKEREGYGGGG 807
>BG653461
Length = 394
Score = 27.3 bits (59), Expect = 2.9
Identities = 16/52 (30%), Positives = 26/52 (49%)
Frame = +2
Query: 71 KKLLSNISNKALSQFGKKKQREEREKEGWGNGGVWQKEILMGGKCEPLDFSG 122
KK K + KKK++++++K+ WG G K L GG P ++ G
Sbjct: 2 KKKKKKKKKKKKKKKKKKKKKKKKKKKKWGEGKK-TKIPLRGGPITPFNWRG 154
>TC207509
Length = 641
Score = 27.3 bits (59), Expect = 2.9
Identities = 15/52 (28%), Positives = 25/52 (47%), Gaps = 1/52 (1%)
Frame = +1
Query: 6 SPESPNRHHSNGGFFVTISAILALLSRKANRLKEKAKSSSTTKPI-RDEEWR 56
+P SPN HH N ++ + L R+ +A++S+T + R WR
Sbjct: 220 TPSSPNPHHPNLNLHPNLNHNPSPLPRRTRNALGRARTSAT*RTCRRSRRWR 375
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.313 0.133 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,176,206
Number of Sequences: 63676
Number of extensions: 92879
Number of successful extensions: 605
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 597
length of query: 153
length of database: 12,639,632
effective HSP length: 89
effective length of query: 64
effective length of database: 6,972,468
effective search space: 446237952
effective search space used: 446237952
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC148996.15