BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148994.1 + phase: 0
(347 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218780 similar to UP|Q9FFD2 (Q9FFD2) Amylogenin; reversibly gl... 391 e-109
TC225386 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated... 365 e-101
TC225385 similar to UP|Q6IV07 (Q6IV07) UDP-glucose:protein trans... 362 e-100
TC225387 homologue to UP|O24548 (O24548) Type IIIa membrane prot... 186 1e-47
TC225388 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated... 173 1e-43
TC225383 homologue to UP|O24547 (O24547) Type IIIa membrane prot... 120 7e-28
AW597016 similar to PIR|T11576|T11 type IIIa membrane protein cp... 116 1e-26
BG154238 similar to GP|9759135|dbj| amylogenin; reversibly glyco... 115 4e-26
TC220756 similar to UP|Q9FFD2 (Q9FFD2) Amylogenin; reversibly gl... 89 2e-18
TC225384 similar to UP|O24547 (O24547) Type IIIa membrane protei... 63 2e-10
CA802654 similar to GP|4158232|emb| reversibly glycosylated poly... 60 1e-09
AW310021 similar to GP|18077708|emb reversibly glycosylated poly... 55 5e-08
AW203319 similar to PIR|T11576|T115 type IIIa membrane protein c... 40 0.002
TC214733 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane ph... 29 3.6
>TC218780 similar to UP|Q9FFD2 (Q9FFD2) Amylogenin; reversibly glycosylatable
polypeptide (Amylogenin), partial (61%)
Length = 884
Score = 391 bits (1004), Expect = e-109
Identities = 187/214 (87%), Positives = 200/214 (93%)
Frame = +2
Query: 133 FNTLYDPFRKGADFVRGYPFSLRSGVDCALSCGLWLNLADLDAPTQALKPTQRNSRYVDA 192
FNTLYDPF KGADFVRGYPFSLRSGVDCALSCGLWLNLADLDAPTQALKP QRN RYVDA
Sbjct: 2 FNTLYDPFCKGADFVRGYPFSLRSGVDCALSCGLWLNLADLDAPTQALKPGQRNLRYVDA 181
Query: 193 VLTVPTRAMLPVSGINIAFNRELVGPALVPALVLAGEGKLRWETVEDIWCGLCVKIVCDH 252
VLTVP+RAM+PVSGINIAFNRE VGPALVPAL LAGEGKLRWET+EDIWCG+CVK++CDH
Sbjct: 182 VLTVPSRAMVPVSGINIAFNREAVGPALVPALRLAGEGKLRWETMEDIWCGMCVKVICDH 361
Query: 253 LSLGVKSGLPYVWRNERGNAIDSLKKEWEGVKLMEDVVPFFQSVKLPQSATTAEDCVIEM 312
L LGVKSGLPYVWR ERG+AI SLKKEWEGVKLMEDVVPFFQS++LPQSATTAE CV+E+
Sbjct: 362 LGLGVKSGLPYVWRTERGDAIQSLKKEWEGVKLMEDVVPFFQSLRLPQSATTAEACVVEV 541
Query: 313 AKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGS 346
AK+VKE LGKVDPMF +AA AM EWVKLWKSV S
Sbjct: 542 AKTVKEHLGKVDPMFSEAAQAMEEWVKLWKSVRS 643
>TC225386 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated
polypeptide, partial (94%)
Length = 1547
Score = 365 bits (937), Expect = e-101
Identities = 169/339 (49%), Positives = 239/339 (69%), Gaps = 1/339 (0%)
Frame = +1
Query: 9 NEVDIVIGALHSNLTPFMNEWKSIFSRFHLIIVKDPALKEELQIPEGFSADVYTNSEIER 68
+E+DIVI + + F+ W+ F +HLIIV+D +++P+GF ++Y ++I R
Sbjct: 112 DELDIVIPTIRN--LDFLEMWRPFFEPYHLIIVQDGDPNRTIKVPDGFDYELYNRNDINR 285
Query: 69 VVGSSTS-IRFSGYACRYFGFLVSKKKYVVCIDDDCVPAKDDAGNVVDAVAQHIVNLKTP 127
++G S I F ACR FG++VSKKKY+ IDDDC AKD +G ++A+ QHI NL P
Sbjct: 286 ILGPKASCISFKDSACRCFGYMVSKKKYIYTIDDDCFVAKDPSGKDINALEQHIKNLLCP 465
Query: 128 ATPFFFNTLYDPFRKGADFVRGYPFSLRSGVDCALSCGLWLNLADLDAPTQALKPTQRNS 187
+TPFFFNTLYDP+R GADFVRGYPFSLR G A+S GLWLN+ D DAPTQ +KP +RN+
Sbjct: 466 STPFFFNTLYDPYRAGADFVRGYPFSLREGAPTAVSHGLWLNIPDYDAPTQLVKPLERNT 645
Query: 188 RYVDAVLTVPTRAMLPVSGINIAFNRELVGPALVPALVLAGEGKLRWETVEDIWCGLCVK 247
RYVDAVLT+P + P+ G+N+AF+R+L+GPA+ L+ G+ R+ +D+W G CVK
Sbjct: 646 RYVDAVLTIPKGTLFPMCGMNLAFDRQLIGPAMYFGLMGDGQPIGRY---DDMWAGWCVK 816
Query: 248 IVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEGVKLMEDVVPFFQSVKLPQSATTAED 307
++CDHL LGVK+GLPY+W ++ N +LKKE++G+ E+++PFFQS L + T+ +
Sbjct: 817 VICDHLGLGVKTGLPYIWHSKASNPFVNLKKEYKGIFWQEEIIPFFQSATLSKECTSVQK 996
Query: 308 CVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGS 346
C IE++K VKE+LG VDP F+K ADAM W++ W + +
Sbjct: 997 CYIELSKQVKEKLGAVDPYFIKLADAMVTWIEAWDELNN 1113
>TC225385 similar to UP|Q6IV07 (Q6IV07) UDP-glucose:protein
transglucosylase-like protein SlUPTG1, partial (95%)
Length = 1298
Score = 362 bits (928), Expect = e-100
Identities = 168/340 (49%), Positives = 242/340 (70%), Gaps = 1/340 (0%)
Frame = +3
Query: 9 NEVDIVIGALHSNLTPFMNEWKSIFSRFHLIIVKDPALKEELQIPEGFSADVYTNSEIER 68
+E+DIVI + + F+ W+ F +HLIIV+D + +++PEGF ++Y ++I R
Sbjct: 108 DELDIVIPTIRN--LDFLEMWRPFFEPYHLIIVQDGDPSKIIKVPEGFDYELYNRNDINR 281
Query: 69 VVGSSTS-IRFSGYACRYFGFLVSKKKYVVCIDDDCVPAKDDAGNVVDAVAQHIVNLKTP 127
++G + I F ACR FG++VSKKKY+ IDDDC A D +G+ ++A+ QHI NL P
Sbjct: 282 ILGPKANCISFKDSACRCFGYMVSKKKYIYTIDDDCFVATDPSGHKINALKQHIENLLCP 461
Query: 128 ATPFFFNTLYDPFRKGADFVRGYPFSLRSGVDCALSCGLWLNLADLDAPTQALKPTQRNS 187
+TP+FFNTLY+PFR+GADFVRGYPFSLR GV A+S GLWLN+ D DAPTQ +KP +RN+
Sbjct: 462 STPYFFNTLYEPFREGADFVRGYPFSLREGVPTAVSHGLWLNIPDYDAPTQLVKPLERNT 641
Query: 188 RYVDAVLTVPTRAMLPVSGINIAFNRELVGPALVPALVLAGEGKLRWETVEDIWCGLCVK 247
RYVDA+LT+P + P+ G+N+AF+R+L+G A+ L+ G+ R+ +D+W G C K
Sbjct: 642 RYVDAILTIPKGTLFPMCGMNLAFDRDLIGAAMYFGLMGDGQPIGRY---DDMWAGWCCK 812
Query: 248 IVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEGVKLMEDVVPFFQSVKLPQSATTAED 307
++CDHL LG+K+GLPY++ ++ N +L+KE++G+ ED++PFFQS+ LP+ ATT +
Sbjct: 813 VICDHLGLGIKTGLPYIYHSKASNPFVNLRKEYKGIFWQEDIIPFFQSIVLPKEATTVQK 992
Query: 308 CVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGSA 347
C IE+AK VKE+L KVDP F K ADAM W++ W + A
Sbjct: 993 CYIELAKQVKEKLSKVDPYFDKLADAMVTWIEAWDELNPA 1112
>TC225387 homologue to UP|O24548 (O24548) Type IIIa membrane protein
cp-wap13, partial (49%)
Length = 823
Score = 186 bits (472), Expect = 1e-47
Identities = 81/170 (47%), Positives = 122/170 (71%)
Frame = +1
Query: 177 TQALKPTQRNSRYVDAVLTVPTRAMLPVSGINIAFNRELVGPALVPALVLAGEGKLRWET 236
T+ +KP +RN+RYVDAVLT+P + P+ G+N+AF+R+L+GPA+ L+ G+ R++
Sbjct: 1 TRLVKPLERNTRYVDAVLTIPKGTLFPMCGMNLAFDRQLIGPAMYFGLMGDGQPIGRYD- 177
Query: 237 VEDIWCGLCVKIVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEGVKLMEDVVPFFQSV 296
D+W G CVK++CDHL LGVK+GLPY+W ++ N +LKKE++G+ E+++PFFQS
Sbjct: 178 --DMWAGWCVKVICDHLGLGVKTGLPYIWHSKASNPFVNLKKEYKGIFWQEEIIPFFQSA 351
Query: 297 KLPQSATTAEDCVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGS 346
+P+ T+ + C IE++K VKE+LG VDP F K ADAM W++ W + S
Sbjct: 352 TIPKECTSVQKCYIELSKQVKEKLGAVDPYFTKLADAMVTWIEAWDELNS 501
>TC225388 homologue to UP|Q9ZR33 (Q9ZR33) Reversibly glycosylated
polypeptide, partial (46%)
Length = 546
Score = 173 bits (438), Expect = 1e-43
Identities = 83/160 (51%), Positives = 110/160 (67%), Gaps = 1/160 (0%)
Frame = +2
Query: 9 NEVDIVIGALHSNLTPFMNEWKSIFSRFHLIIVKDPALKEELQIPEGFSADVYTNSEIER 68
+E+DIVI + + F+ W+ F +HLIIV+D + +PEGF ++Y ++I R
Sbjct: 71 DELDIVIPTIRN--LDFLEMWRPFFEPYHLIIVQDGDPNRTINVPEGFDYELYNRNDINR 244
Query: 69 VVGSSTS-IRFSGYACRYFGFLVSKKKYVVCIDDDCVPAKDDAGNVVDAVAQHIVNLKTP 127
++G S I F ACR FG++VSKKKY+ IDDDC AKD +G ++A+ QHI NL P
Sbjct: 245 ILGPKASCISFKDSACRCFGYMVSKKKYIYTIDDDCFVAKDPSGKDINALEQHIKNLLCP 424
Query: 128 ATPFFFNTLYDPFRKGADFVRGYPFSLRSGVDCALSCGLW 167
+TPFFFNTLYDP+R+GADFVRGYPFSLR G A+S GLW
Sbjct: 425 STPFFFNTLYDPYREGADFVRGYPFSLREGAPTAVSHGLW 544
>TC225383 homologue to UP|O24547 (O24547) Type IIIa membrane protein
cp-wap11, partial (37%)
Length = 775
Score = 120 bits (302), Expect = 7e-28
Identities = 51/104 (49%), Positives = 75/104 (72%)
Frame = +3
Query: 244 LCVKIVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEGVKLMEDVVPFFQSVKLPQSAT 303
+ V ++CDHL LG+K+GLPY++ ++ N +L+KE++G+ ED++PFFQS+ LP+ AT
Sbjct: 108 MIV*VICDHLGLGIKTGLPYIYHSKASNPFVNLRKEYKGIFWQEDIIPFFQSIVLPKEAT 287
Query: 304 TAEDCVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGSA 347
T + C IE+AK VKE+L KVDP F K ADAM W++ W + A
Sbjct: 288 TVQKCYIELAKQVKEKLSKVDPYFDKLADAMVTWIEAWDELNPA 419
>AW597016 similar to PIR|T11576|T11 type IIIa membrane protein cp-wap11 -
cowpea, partial (45%)
Length = 445
Score = 116 bits (291), Expect = 1e-26
Identities = 56/148 (37%), Positives = 92/148 (61%)
Frame = +2
Query: 163 SCGLWLNLADLDAPTQALKPTQRNSRYVDAVLTVPTRAMLPVSGINIAFNRELVGPALVP 222
S GLWL +A DAPTQ L P +RN+ YVDA+ T+P ++P+ G+N++F+R L+ A+
Sbjct: 11 SHGLWLYIAYYDAPTQLLNPLERNTTYVDAIWTIPKGTLIPMCGMNLSFDR*LI*AAIYS 190
Query: 223 ALVLAGEGKLRWETVEDIWCGLCVKIVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEG 282
L+ G R+ +W G C K++C+H L + +GLPY++ ++ + +L+K++
Sbjct: 191 GLMGDGHTIRRY---HHMWAGWCCKVICEH*RLTINTGLPYIYHSKASHPWVNLRKDYIC 361
Query: 283 VKLMEDVVPFFQSVKLPQSATTAEDCVI 310
+ ED++ FFQS+ LP +TA C I
Sbjct: 362 LFWQEDIIHFFQSIDLPTETSTAPICYI 445
>BG154238 similar to GP|9759135|dbj| amylogenin; reversibly glycosylatable
polypeptide {Arabidopsis thaliana}, partial (18%)
Length = 220
Score = 115 bits (287), Expect = 4e-26
Identities = 52/70 (74%), Positives = 58/70 (82%)
Frame = +2
Query: 224 LVLAGEGKLRWETVEDIWCGLCVKIVCDHLSLGVKSGLPYVWRNERGNAIDSLKKEWEGV 283
L LAGEGKLRWET+EDIWCG+CVK++CDHL LGVKSG PYV R +RG+AI S KEWE V
Sbjct: 2 LRLAGEGKLRWETMEDIWCGMCVKVICDHLGLGVKSGSPYVRRTQRGDAIQSSNKEWERV 181
Query: 284 KLMEDVVPFF 293
KLMED FF
Sbjct: 182 KLMEDASIFF 211
>TC220756 similar to UP|Q9FFD2 (Q9FFD2) Amylogenin; reversibly glycosylatable
polypeptide (Amylogenin), partial (14%)
Length = 774
Score = 89.4 bits (220), Expect = 2e-18
Identities = 52/84 (61%), Positives = 60/84 (70%), Gaps = 2/84 (2%)
Frame = +3
Query: 1 MSQVIINDNEVDIVIGALHSNLTPFMNEWKSIFSRFHLIIVKDPALKEEL-QIPEGFSAD 59
MSQ +INDNEVDIVIGALHS+LT FMNEW+ IFSRFHLIIVKDP LKE + + D
Sbjct: 429 MSQAVINDNEVDIVIGALHSDLTTFMNEWRPIFSRFHLIIVKDPDLKEGTPKFL*ASTLD 608
Query: 60 VYTN-SEIERVVGSSTSIRFSGYA 82
V+T SE ++V G S F G A
Sbjct: 609 VFTKASESDQVGGG--SFHFXGXA 674
>TC225384 similar to UP|O24547 (O24547) Type IIIa membrane protein cp-wap11,
partial (18%)
Length = 530
Score = 62.8 bits (151), Expect = 2e-10
Identities = 29/53 (54%), Positives = 36/53 (67%)
Frame = +2
Query: 295 SVKLPQSATTAEDCVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVGSA 347
SV LP+ ATT + C IE+AK VKE+L KVDP F K ADAM W++ W + A
Sbjct: 2 SVVLPKEATTVQKCYIELAKQVKEKLTKVDPYFDKLADAMVTWIEAWDELNPA 160
>CA802654 similar to GP|4158232|emb| reversibly glycosylated polypeptide
{Triticum aestivum}, partial (16%)
Length = 396
Score = 60.1 bits (144), Expect = 1e-09
Identities = 28/52 (53%), Positives = 35/52 (66%), Gaps = 1/52 (1%)
Frame = +1
Query: 53 PEGFSADVYTNSEIERVVGSSTS-IRFSGYACRYFGFLVSKKKYVVCIDDDC 103
PEGF ++Y ++I RV+G I F ACR FGFL+SKKKY+ IDDDC
Sbjct: 25 PEGFDYELYNRNDINRVLGPKAHCISFKDSACRCFGFLLSKKKYIFTIDDDC 180
>AW310021 similar to GP|18077708|emb reversibly glycosylated polypeptide
{Gossypium hirsutum}, partial (22%)
Length = 244
Score = 55.1 bits (131), Expect = 5e-08
Identities = 28/77 (36%), Positives = 45/77 (58%), Gaps = 1/77 (1%)
Frame = +2
Query: 36 FHLIIVKDPALKEELQIPEGFSADVYTNSEIERVVGSST-SIRFSGYACRYFGFLVSKKK 94
++LII D + + +PEG++ ++Y ++I R++G I F A FG++VSK K
Sbjct: 5 YNLIIEHD*NPNKTI*VPEGYNYELYIRNDINRILGPKAYCISFKDSA*NCFGYMVSKNK 184
Query: 95 YVVCIDDDCVPAKDDAG 111
Y+ ID DC AK+ G
Sbjct: 185 YIYAIDKDCFDAKNPYG 235
>AW203319 similar to PIR|T11576|T115 type IIIa membrane protein cp-wap11 -
cowpea, partial (13%)
Length = 418
Score = 40.0 bits (92), Expect = 0.002
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = +2
Query: 181 KPTQRNSRYVDAVLTVPTRAMLPVSGINIAFNRELVGPAL 220
K +RN+ VDA +T+ + P+ G+N+A +R+L+GPA+
Sbjct: 284 KALERNTMNVDADITIAIGTLFPMCGVNVA*DRQLIGPAM 403
>TC214733 similar to UP|TP14_ARATH (Q8LCA1) Thylakoid membrane phosphoprotein
14 kDa, chloroplast precursor, partial (56%)
Length = 1021
Score = 28.9 bits (63), Expect = 3.6
Identities = 17/47 (36%), Positives = 26/47 (55%)
Frame = +2
Query: 299 PQSATTAEDCVIEMAKSVKEQLGKVDPMFLKAADAMAEWVKLWKSVG 345
P AT E V E+ K+++E KVD + ++ A+ + LW SVG
Sbjct: 350 PAEATPTE--VPEIVKTLQETWDKVDDKYAVSSLALVGVIALWGSVG 484
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.137 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,816,318
Number of Sequences: 63676
Number of extensions: 219218
Number of successful extensions: 983
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 972
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 975
length of query: 347
length of database: 12,639,632
effective HSP length: 98
effective length of query: 249
effective length of database: 6,399,384
effective search space: 1593446616
effective search space used: 1593446616
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148994.1