BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148971.3 - phase: 0
(226 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC227612 weakly similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_1... 194 4e-50
TC227613 175 2e-44
TC206373 similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_12, part... 103 8e-23
TC234259 similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_12, part... 64 4e-11
TC221693 63 1e-10
TC234666 62 2e-10
BE802665 59 1e-09
TC210855 34 0.047
TC221816 weakly similar to UP|Q70G10 (Q70G10) SUMO protease, par... 33 0.14
BF066760 similar to GP|9293943|dbj| contains similarity to DNA-b... 30 0.89
TC218813 30 0.89
TC216000 similar to UP|O22253 (O22253) Photolyase/blue-light rec... 29 1.5
TC225311 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal prote... 28 2.6
TC225310 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal prote... 28 3.4
BQ253804 similar to GP|10176854|dbj pectinacetylesterase {Arabid... 28 4.4
BE611936 28 4.4
BM307520 28 4.4
TC218760 similar to UP|O04098 (O04098) Receptor-kinase isolog, 5... 28 4.4
TC230392 27 5.8
TC223840 similar to GB|AAR92297.1|40823665|BT011261 At2g45860 {A... 27 5.8
>TC227612 weakly similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_12, partial
(19%)
Length = 1563
Score = 194 bits (492), Expect = 4e-50
Identities = 114/224 (50%), Positives = 129/224 (56%), Gaps = 1/224 (0%)
Frame = +1
Query: 1 MADDFSSKFLQLRFISLEVSSLQMNLTNSVELFIDFCFSLPPFFFFQLPQQDNFYDCGLF 60
+ +D SSKFL LRFISLE LPQQ+N YDCGLF
Sbjct: 187 VVEDVSSKFLHLRFISLE-----------------------------LPQQENLYDCGLF 279
Query: 61 LLYFVERFLEEAPIKFNPFKITKFSKFLNSNWFPSDEASLRRSHIQNLIYDIFENGSLKA 120
LL++VERFLEEAPI FNPF ITK S FLNSNWFP E SL+RSHIQ++IYDIFEN SL+A
Sbjct: 280 LLHYVERFLEEAPINFNPFMITKSSIFLNSNWFPPLEVSLKRSHIQSVIYDIFENNSLQA 459
Query: 121 PPIDCRGKGPLSELPGVIEH-KVEADSSGASCYPGIWHGNLSNGSTETDIQFRPVSPVRA 179
P DC K L E P +I + K+E DS CYP SP+R
Sbjct: 460 PHTDCLDKDLLLEDPAIIVNPKLEEDSLRGCCYP-------------------TASPIRV 582
Query: 180 ASCSRDPGIVFKDLQAAVVPPHFDCRQMSLCHQSGFLSPIEVFE 223
ASC PG+V KDL AVV C QMS CHQ GFLSP+E E
Sbjct: 583 ASCLTGPGLVSKDL-VAVVTSRSACLQMSACHQRGFLSPLEEIE 711
>TC227613
Length = 1444
Score = 175 bits (443), Expect = 2e-44
Identities = 95/153 (62%), Positives = 104/153 (67%), Gaps = 5/153 (3%)
Frame = +1
Query: 76 FNPFKITKFSKFLNSNWFPSDEASLRRSHIQNLIYDIFENGSLKAPPIDCRGKGPLSELP 135
FNPF ITK S FL+SNWFP EASL+RSHIQNLIYDIFEN SL APP DC KG SE P
Sbjct: 4 FNPFMITKSSNFLSSNWFPPPEASLKRSHIQNLIYDIFENNSLHAPPTDCLDKGHPSEDP 183
Query: 136 GVIEH-KVEADSSGASCYPGIWHG-NLSNGSTE---TDIQFRPVSPVRAASCSRDPGIVF 190
+I + KVE DS CYP +WHG N SN STE TDIQ+ S +R C G+V
Sbjct: 184 SIIVNPKVEEDSL-RGCYPALWHGKNPSNSSTELETTDIQYPTASHIRVPICLTGSGLVS 360
Query: 191 KDLQAAVVPPHFDCRQMSLCHQSGFLSPIEVFE 223
KDLQAAVV H DC QM CHQ GFLSP+E E
Sbjct: 361 KDLQAAVVTSHSDCLQMPACHQRGFLSPLEEIE 459
>TC206373 similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_12, partial (18%)
Length = 1651
Score = 103 bits (256), Expect = 8e-23
Identities = 56/128 (43%), Positives = 74/128 (57%), Gaps = 1/128 (0%)
Frame = +3
Query: 1 MADDFSSKFLQLRFISLEVSSLQMNLTNSVELFIDFCFSLPPFFFFQLPQQDNFYDCGLF 60
+ +D SS+FL +RF+ L LPQQ+N YDCGLF
Sbjct: 159 LGEDLSSRFLNMRFLPLA-----------------------------LPQQENSYDCGLF 251
Query: 61 LLYFVERFLEEAPIKFNPFKITKFSKFLNSNWFPSDEASLRRSHIQNLIYDIFEN-GSLK 119
LL+++E FL EAP+ FNPFK+TKFS FLN +WF EA L+R+ IQ LI+++ EN GS +
Sbjct: 252 LLHYLELFLAEAPLNFNPFKLTKFSNFLNVDWFLPAEAFLKRTLIQKLIFELLENHGSHE 431
Query: 120 APPIDCRG 127
DC G
Sbjct: 432 ISSSDCSG 455
>TC234259 similar to UP|Q8L7S0 (Q8L7S0) At1g09730/F21M12_12, partial (8%)
Length = 468
Score = 64.3 bits (155), Expect = 4e-11
Identities = 29/39 (74%), Positives = 33/39 (84%)
Frame = +2
Query: 51 QDNFYDCGLFLLYFVERFLEEAPIKFNPFKITKFSKFLN 89
QDN YDCGLFLL++VE FLEEAPI FNPF ITK S F++
Sbjct: 68 QDNLYDCGLFLLHYVEGFLEEAPINFNPFMITKSSIFVS 184
>TC221693
Length = 760
Score = 62.8 bits (151), Expect = 1e-10
Identities = 31/69 (44%), Positives = 42/69 (59%)
Frame = +1
Query: 47 QLPQQDNFYDCGLFLLYFVERFLEEAPIKFNPFKITKFSKFLNSNWFPSDEASLRRSHIQ 106
Q+PQQ N YDCGLF+LYF+ERF+EEAP + + F + WF EAS R I+
Sbjct: 142 QVPQQKNDYDCGLFVLYFIERFMEEAPERLKMKDLDMFGR----RWFKPQEASNLRVKIR 309
Query: 107 NLIYDIFEN 115
L+ + +N
Sbjct: 310 KLLVEKLQN 336
>TC234666
Length = 725
Score = 62.0 bits (149), Expect = 2e-10
Identities = 32/69 (46%), Positives = 41/69 (59%)
Frame = +1
Query: 47 QLPQQDNFYDCGLFLLYFVERFLEEAPIKFNPFKITKFSKFLNSNWFPSDEASLRRSHIQ 106
Q+PQQ N YDCGLF+LYF+ERF+EEAP + F + WF EAS R I
Sbjct: 190 QVPQQKNEYDCGLFVLYFIERFMEEAPE*LKRKDLDMFGR----RWFKPQEASNLRVKIL 357
Query: 107 NLIYDIFEN 115
L+ +I +N
Sbjct: 358 KLLLEILQN 384
>BE802665
Length = 370
Score = 59.3 bits (142), Expect = 1e-09
Identities = 30/63 (47%), Positives = 39/63 (61%)
Frame = +1
Query: 47 QLPQQDNFYDCGLFLLYFVERFLEEAPIKFNPFKITKFSKFLNSNWFPSDEASLRRSHIQ 106
Q+PQQ N YDCGLF+LYF+ERF+EEAP + + F + WF EAS R I+
Sbjct: 82 QVPQQKNDYDCGLFVLYFIERFMEEAPERLKMKDLDMFGR----RWFKPQEASNLRVKIR 249
Query: 107 NLI 109
L+
Sbjct: 250 ILL 258
>TC210855
Length = 908
Score = 34.3 bits (77), Expect = 0.047
Identities = 17/59 (28%), Positives = 32/59 (53%), Gaps = 5/59 (8%)
Frame = +3
Query: 32 LFIDFCFSLPPFFFFQLPQQDNFYDCGLFLLYFVERFLEEAP-----IKFNPFKITKFS 85
L++D + FF+F P+ NF+ C +F+ +F+ +L+ FNPF++ F+
Sbjct: 291 LYLDHWVTFFLFFYFSQPKSLNFFGCSVFIFFFL*CWLKLFQWVIFLYLFNPFELDTFN 467
>TC221816 weakly similar to UP|Q70G10 (Q70G10) SUMO protease, partial (28%)
Length = 943
Score = 32.7 bits (73), Expect = 0.14
Identities = 13/43 (30%), Positives = 24/43 (55%)
Frame = +3
Query: 44 FFFQLPQQDNFYDCGLFLLYFVERFLEEAPIKFNPFKITKFSK 86
F LP+Q N YDCG+F++ + + + + FN ++ F +
Sbjct: 495 FVEDLPEQQNGYDCGVFMIKYADFYSRNLGLCFNQEHMSYFRR 623
>BF066760 similar to GP|9293943|dbj| contains similarity to DNA-binding
protein~gene_id:MYM9.3 {Arabidopsis thaliana}, partial
(6%)
Length = 448
Score = 30.0 bits (66), Expect = 0.89
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -2
Query: 146 SSGASCYPGIWHGNLSNGSTE 166
SSG +C WH +SNGSTE
Sbjct: 285 SSGDNCMARFWHKEVSNGSTE 223
>TC218813
Length = 776
Score = 30.0 bits (66), Expect = 0.89
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -2
Query: 146 SSGASCYPGIWHGNLSNGSTE 166
SSG +C WH +SNGSTE
Sbjct: 223 SSGDNCMARFWHKEVSNGSTE 161
>TC216000 similar to UP|O22253 (O22253) Photolyase/blue-light receptor
(Photolyase/blue light photoreceptor PHR2), partial
(84%)
Length = 1641
Score = 29.3 bits (64), Expect = 1.5
Identities = 28/101 (27%), Positives = 45/101 (43%)
Frame = +2
Query: 126 RGKGPLSELPGVIEHKVEADSSGASCYPGIWHGNLSNGSTETDIQFRPVSPVRAASCSRD 185
RG L E+P +++ + + S G + N S ET+ R AA C+
Sbjct: 794 RGDFELGEIPSLMD--LGLNPSATMSQDGKFGANASMIGGETEALQRLKK--FAAECAAQ 961
Query: 186 PGIVFKDLQAAVVPPHFDCRQMSLCHQSGFLSPIEVFETLQ 226
P FKD ++ +F C+ +S G LSP ++E L+
Sbjct: 962 PNKGFKDGTQSIYGANFSCK-ISPWLAMGCLSPRTMYEELK 1081
>TC225311 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal protein S25-1,
complete
Length = 729
Score = 28.5 bits (62), Expect = 2.6
Identities = 15/27 (55%), Positives = 16/27 (58%), Gaps = 1/27 (3%)
Frame = -1
Query: 27 TNSVELF-IDFCFSLPPFFFFQLPQQD 52
+NS LF FCF L FFFF LP D
Sbjct: 225 SNSTMLFTFSFCFPLLHFFFFCLPPPD 145
>TC225310 homologue to UP|R25A_ARATH (Q9SIK2) 40S ribosomal protein S25-1,
complete
Length = 700
Score = 28.1 bits (61), Expect = 3.4
Identities = 15/27 (55%), Positives = 16/27 (58%), Gaps = 1/27 (3%)
Frame = -2
Query: 27 TNSVELF-IDFCFSLPPFFFFQLPQQD 52
+NS LF FCF L FFFF LP D
Sbjct: 213 SNSTILFTFSFCFPLLHFFFFCLPPPD 133
>BQ253804 similar to GP|10176854|dbj pectinacetylesterase {Arabidopsis
thaliana}, partial (15%)
Length = 423
Score = 27.7 bits (60), Expect = 4.4
Identities = 15/48 (31%), Positives = 24/48 (49%), Gaps = 6/48 (12%)
Frame = -1
Query: 164 STETDIQFRPVSP------VRAASCSRDPGIVFKDLQAAVVPPHFDCR 205
+++T + FRP + ASCSR+ +V + A+ PP D R
Sbjct: 303 ASDTPVNFRPNKERH*HERLERASCSREASVVVVASKRAMAPP*IDTR 160
>BE611936
Length = 416
Score = 27.7 bits (60), Expect = 4.4
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = -3
Query: 148 GASCYPGIWHGNLSNGSTE 166
G +C WH +SNGSTE
Sbjct: 378 GGNCMARFWHKEVSNGSTE 322
>BM307520
Length = 402
Score = 27.7 bits (60), Expect = 4.4
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +1
Query: 43 FFFFQLPQQDNFYDCGLFLLYFV 65
FF+F P+ NF+ C F+ +F+
Sbjct: 304 FFYFS*PKSLNFFGCSAFIYFFL 372
>TC218760 similar to UP|O04098 (O04098) Receptor-kinase isolog, 5' partial;
115640-113643 (Fragment), partial (50%)
Length = 1236
Score = 27.7 bits (60), Expect = 4.4
Identities = 24/85 (28%), Positives = 33/85 (38%), Gaps = 4/85 (4%)
Frame = +2
Query: 108 LIYDIFENGSLKAPPIDCRGKGPLSELPGVIEHKVEADSSGASCY----PGIWHGNLSNG 163
L+YD F NGSL + + G L K+ D + Y PG+ HGNL +
Sbjct: 317 LVYDYFPNGSLFSLIHGSKTSGGGKPLHWTSCLKIAEDLATGMLYIHQNPGLTHGNLKSS 496
Query: 164 STETDIQFRPVSPVRAASCSRDPGI 188
+ F SC D G+
Sbjct: 497 NVLLGSDFE--------SCLTDYGL 547
>TC230392
Length = 708
Score = 27.3 bits (59), Expect = 5.8
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3
Query: 151 CYPGIWHGNLSNGSTE 166
C P WH +SNGSTE
Sbjct: 610 CMPRFWHKPVSNGSTE 563
>TC223840 similar to GB|AAR92297.1|40823665|BT011261 At2g45860 {Arabidopsis
thaliana;} , complete
Length = 460
Score = 27.3 bits (59), Expect = 5.8
Identities = 20/63 (31%), Positives = 28/63 (43%), Gaps = 5/63 (7%)
Frame = -2
Query: 7 SKFLQLR-FISLEVSSLQMNLTNSVELFIDFCFS----LPPFFFFQLPQQDNFYDCGLFL 61
SK Q+ +S ++ + L +V L F F LPPFFF LP F+
Sbjct: 315 SKITQVEPHLSSSIACAALALARAVNLVFTFLFPT*NPLPPFFFLPLP--------STFI 160
Query: 62 LYF 64
L+F
Sbjct: 159 LFF 151
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.141 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,610,802
Number of Sequences: 63676
Number of extensions: 181364
Number of successful extensions: 1440
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1424
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1432
length of query: 226
length of database: 12,639,632
effective HSP length: 94
effective length of query: 132
effective length of database: 6,654,088
effective search space: 878339616
effective search space used: 878339616
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148971.3