BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148775.11 - phase: 0 /pseudo/partial
(467 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220263 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partia... 266 2e-71
CO979507 245 3e-65
TC229557 weakly similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase ... 175 4e-44
BI970438 weakly similar to SP|Q9LTS3|CKX3 Cytokinin oxidase 3 pr... 169 3e-42
TC232674 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-li... 159 3e-39
BI498566 similar to GP|11120514|gb cytokinin oxidase {Arabidopsi... 130 1e-30
TC222766 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partia... 102 3e-22
BG651837 weakly similar to GP|11120514|gb| cytokinin oxidase {Ar... 89 5e-18
CA936072 weakly similar to GP|11120514|gb| cytokinin oxidase {Ar... 71 1e-12
TC234116 similar to UP|Q9LY71 (Q9LY71) Cytokinin oxidase-like pr... 65 6e-11
BU926889 weakly similar to GP|11120508|gb| cytokinin oxidase {Ar... 60 2e-09
TC220851 similar to UP|Q84U27 (Q84U27) Cytokinin dehydrogenase 3... 55 9e-08
AW755511 similar to GP|11120514|gb| cytokinin oxidase {Arabidops... 47 2e-05
TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like p... 45 9e-05
TC217973 weakly similar to UP|Q93ZA3 (Q93ZA3) At1g30760/T5I8_22,... 39 0.006
BG510985 similar to GP|20279572|gb L-galactono-1 4-lactone dehyd... 31 1.3
BG510024 similar to PIR|T10626|T10 reticuline oxidase homolog F2... 31 1.3
AW306334 weakly similar to GP|23267157|gb| cytokinin dehydrogena... 31 1.3
TC213020 weakly similar to UP|Q8GU71 (Q8GU71) MDR-like ABC trans... 30 2.3
TC222486 30 3.0
>TC220263 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partial (28%)
Length = 693
Score = 266 bits (679), Expect = 2e-71
Identities = 133/228 (58%), Positives = 169/228 (73%), Gaps = 19/228 (8%)
Frame = +1
Query: 95 KNPL-GCYVDVGGEQLWIDVLNATLKHGLTPLSWTDYLYLSVGGTLSNAGIGGQTFRFGP 153
K+PL G Y DVGGEQLWIDVL+ TLKHGL P+SWTDYLYL+VGGTLSNAGI GQ+FR+GP
Sbjct: 10 KDPLMGHYADVGGEQLWIDVLHTTLKHGLAPVSWTDYLYLTVGGTLSNAGISGQSFRYGP 189
Query: 154 QISNVLELDVITGQGNIVTCSQEKNSEVFYAVLGGLGQFGVITRARILLGPAPTRA---- 209
QISNV E+DVITG+G VTCS +KN E+F+AVLGGLGQFGVI RARI L PAP R
Sbjct: 190 QISNVHEMDVITGKGEFVTCSSQKNLELFHAVLGGLGQFGVIARARIALEPAPKRVKWVR 369
Query: 210 -----------NQEHLISFDRRNDSNGADYVEGMLLLNKPPL---ILSFYPPSDHPRITS 255
+QE LIS + R N D++EGMLL+N+ P+ SF+P SDHPRI S
Sbjct: 370 LLYSDFFAFTKDQERLISINGRKQKNALDFLEGMLLMNQGPINNWRSSFFPLSDHPRIAS 549
Query: 256 LVTQYGITYVLELVKYYDTNSQANITEEVDNLVKGLKFVPTFMFQKDV 303
L+T++ I Y LE+ KYYD ++ N+ +E++ L++GL ++P F ++K+V
Sbjct: 550 LITEHSILYCLEVAKYYDEQTELNVDKEIEVLLQGLAYIPGFYYEKNV 693
>CO979507
Length = 593
Score = 245 bits (625), Expect = 3e-65
Identities = 114/198 (57%), Positives = 150/198 (75%)
Frame = -1
Query: 246 PPSDHPRITSLVTQYGITYVLELVKYYDTNSQANITEEVDNLVKGLKFVPTFMFQKDVTY 305
P +DH RI LVT++ + Y LE+ KYYD ++ N+ +E+ L++GL ++P F ++KDV+Y
Sbjct: 593 PLADHARIILLVTKHSVLYCLEVAKYYDGQNENNVDKELQVLLQGLSYIPGFYYEKDVSY 414
Query: 306 EEFLDRVHSEELILRSKGLWDVPHPWLNMFIPKSRISDFNEGVFKGIILKQNISAGIVLV 365
EFL+RV S EL L+S+GLWDVPHPWLN+FIPKS+I +F+ GVFK IILK+NI+ G VLV
Sbjct: 413 FEFLNRVRSGELKLQSQGLWDVPHPWLNLFIPKSQIMEFDSGVFKNIILKRNITTGPVLV 234
Query: 366 YPMNRNKWDDRMSAITPDEDVFYTLALLHAAKEMDEVKTFQAQNHQILQFCNDAGIKVKE 425
YPMNRNKWD+RMSA PDED+FYT+ LH++ D K + AQN +ILQFCN AGIKVK+
Sbjct: 233 YPMNRNKWDNRMSASIPDEDIFYTVGFLHSS-GFDNWKAYDAQNKEILQFCNVAGIKVKQ 57
Query: 426 YLSGNKTHQEWVEHFGAK 443
Y T ++W HFG K
Sbjct: 56 YFPHYCTQEDWANHFGPK 3
>TC229557 weakly similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-linked
oxidoreductase family), partial (13%)
Length = 841
Score = 175 bits (443), Expect = 4e-44
Identities = 92/210 (43%), Positives = 125/210 (58%), Gaps = 4/210 (1%)
Frame = +1
Query: 262 ITYVLELVKYYDTNSQANITE-EVDNLVKGLKFVPTFMFQKDVTYEEFLDRVHSEELILR 320
+ Y LEL +Y + + EVD L+ L+FV F DVTY EFL RV E +
Sbjct: 1 LLYCLELALHYRNQDHPSAVDMEVDRLLGRLRFVEGLKFCVDVTYMEFLLRVKRVEEDAK 180
Query: 321 SKGLWDVPHPWLNMFIPKSRISDFNEGVFKGIILKQNISAGIVLVYPMNRNKWDDRMSAI 380
+ G+WD PHPWLNMF+ KS I++F+ VFK ILK + G +LVYP+ R+KWD R S +
Sbjct: 181 ANGIWDAPHPWLNMFVSKSNIAEFDREVFKK-ILKHGV-GGPILVYPLLRSKWDSRHSVV 354
Query: 381 TPDEDVFYTLALLH---AAKEMDEVKTFQAQNHQILQFCNDAGIKVKEYLSGNKTHQEWV 437
PD ++FY +ALL + + QNH+I+Q C + G K YL ++ + W+
Sbjct: 355 VPDSNIFYIIALLRFIPPPPKGPPTELLVEQNHEIIQLCYNRGFDFKLYLPHYQSQENWM 534
Query: 438 EHFGAKWQQFEERKAKFDPKRILSPGQGIF 467
H+G KW +F ERKA FDP IL+PGQ IF
Sbjct: 535 RHYGDKWTRFVERKANFDPLAILAPGQKIF 624
>BI970438 weakly similar to SP|Q9LTS3|CKX3 Cytokinin oxidase 3 precursor (EC
1.4.3.-) (CKO 3). [Mouse-ear cress] {Arabidopsis
thaliana}, partial (26%)
Length = 773
Score = 169 bits (427), Expect = 3e-42
Identities = 81/136 (59%), Positives = 97/136 (70%)
Frame = -3
Query: 332 LNMFIPKSRISDFNEGVFKGIILKQNISAGIVLVYPMNRNKWDDRMSAITPDEDVFYTLA 391
LN P S+I N GVFK I+LK+N G VLVYPMNRNKW DRMSA PDEDVFYT+
Sbjct: 771 LNCXXPXSQILXXNSGVFKDIVLKRNXXXGPVLVYPMNRNKWYDRMSASIPDEDVFYTVG 592
Query: 392 LLHAAKEMDEVKTFQAQNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERK 451
LH++ D K + AQN +IL+FC DAGI VK+YL + T ++W HFGAKW +F ERK
Sbjct: 591 FLHSSG-FDTWKAYDAQNREILEFCRDAGIMVKQYLPNHSTQEDWTNHFGAKWMKFLERK 415
Query: 452 AKFDPKRILSPGQGIF 467
+FDP+ ILSPGQ IF
Sbjct: 414 HQFDPRMILSPGQKIF 367
>TC232674 similar to UP|Q9FUJ1 (Q9FUJ1) Cytokinin oxidase (FAD-linked
oxidoreductase family), partial (28%)
Length = 779
Score = 159 bits (401), Expect = 3e-39
Identities = 85/196 (43%), Positives = 122/196 (61%), Gaps = 1/196 (0%)
Frame = +2
Query: 12 ASTDYGHIIHENPFAVLEPTSISDIANLINYSNSLPHSFTISPRGQAHSVLGQAMTQNGI 71
A D+G + P AV+ P D+ + + + T++ RG HS+ GQAM + G+
Sbjct: 167 AGKDFGGMKSVKPRAVIRPALAGDVERAVKEAARTTY-LTVAARGNGHSINGQAMAEKGL 343
Query: 72 VVNMTQLNWYRNGSGIVVSDCDVKNPLGCYVDVGGEQLWIDVLNATLKH-GLTPLSWTDY 130
V++M + + + + + D + YVDV G LW DVL + L P SWTDY
Sbjct: 344 VLDMRAME--DHFTLLSLDDGSL------YVDVSGGALWEDVLKRCVSEFRLAPRSWTDY 499
Query: 131 LYLSVGGTLSNAGIGGQTFRFGPQISNVLELDVITGQGNIVTCSQEKNSEVFYAVLGGLG 190
L L+VGGTLSNAG+ GQ FR+GPQ +NV EL+V++G+G + CS+ +NSE+F+A LGGLG
Sbjct: 500 LGLTVGGTLSNAGVSGQAFRYGPQTANVTELEVVSGKGETLVCSESQNSELFFATLGGLG 679
Query: 191 QFGVITRARILLGPAP 206
QFG+ITRAR+ + AP
Sbjct: 680 QFGIITRARVPVQQAP 727
>BI498566 similar to GP|11120514|gb cytokinin oxidase {Arabidopsis thaliana},
partial (22%)
Length = 378
Score = 130 bits (327), Expect = 1e-30
Identities = 62/106 (58%), Positives = 80/106 (74%), Gaps = 1/106 (0%)
Frame = +3
Query: 102 VDVGGEQLWIDVLNATLKH-GLTPLSWTDYLYLSVGGTLSNAGIGGQTFRFGPQISNVLE 160
VDV G LW +VL + L P SWTDYL L+VGGTLSN G+ GQ+FR+GPQ +NV E
Sbjct: 18 VDVSGGALWEEVLKRCVSEFRLAPRSWTDYLGLTVGGTLSNGGVSGQSFRYGPQTANVTE 197
Query: 161 LDVITGQGNIVTCSQEKNSEVFYAVLGGLGQFGVITRARILLGPAP 206
L+V+TG+G + CS+ +NSE+F+A LGGLGQFG+ITRAR+ + AP
Sbjct: 198 LEVVTGKGETLVCSESQNSELFFATLGGLGQFGIITRARLPVQQAP 335
>TC222766 similar to UP|Q9FUJ0 (Q9FUJ0) Cytokinin oxidase, partial (16%)
Length = 276
Score = 102 bits (255), Expect = 3e-22
Identities = 46/86 (53%), Positives = 66/86 (76%)
Frame = -3
Query: 280 ITEEVDNLVKGLKFVPTFMFQKDVTYEEFLDRVHSEELILRSKGLWDVPHPWLNMFIPKS 339
+ +E+ L+K L F+PT +F D+ + ++LDRVH EL L+SKGLWDV HPWLN+F+PKS
Sbjct: 253 VMQEIQALLKKLNFIPTSVFTTDLPHVDYLDRVHKAELKLKSKGLWDVHHPWLNLFVPKS 74
Query: 340 RISDFNEGVFKGIILKQNISAGIVLV 365
RI DF++GVFKGI+ N ++G +L+
Sbjct: 73 RIEDFDKGVFKGIL--GNKTSGPILI 2
>BG651837 weakly similar to GP|11120514|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (15%)
Length = 414
Score = 88.6 bits (218), Expect = 5e-18
Identities = 46/103 (44%), Positives = 62/103 (59%)
Frame = +2
Query: 271 YYDTNSQANITEEVDNLVKGLKFVPTFMFQKDVTYEEFLDRVHSEELILRSKGLWDVPHP 330
Y + + + + +VD L+ L+F+ FQ DVTY EFL RV E + G WD PHP
Sbjct: 14 YRNADHPSRVDTDVDGLLGRLRFIQGLKFQVDVTYMEFLLRVKRVEEHAKGNGTWDAPHP 193
Query: 331 WLNMFIPKSRISDFNEGVFKGIILKQNISAGIVLVYPMNRNKW 373
WLN+F+ KS I DF+ VFK ILK + G +LVYP+ RN +
Sbjct: 194 WLNLFVSKSHIVDFDREVFKK-ILKDGVD-GPILVYPLLRNNY 316
>CA936072 weakly similar to GP|11120514|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (12%)
Length = 421
Score = 70.9 bits (172), Expect = 1e-12
Identities = 30/60 (50%), Positives = 40/60 (66%)
Frame = +1
Query: 408 QNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERKAKFDPKRILSPGQGIF 467
QNH+I+Q C + G K YL ++ + W+ H+G KW +F ERKA FDP IL+PGQ IF
Sbjct: 70 QNHEIIQLCYNRGFDFKLYLPHYQSKENWMRHYGDKWSRFVERKANFDPLAILAPGQKIF 249
>TC234116 similar to UP|Q9LY71 (Q9LY71) Cytokinin oxidase-like protein,
partial (10%)
Length = 638
Score = 65.1 bits (157), Expect = 6e-11
Identities = 27/61 (44%), Positives = 40/61 (65%)
Frame = +1
Query: 407 AQNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERKAKFDPKRILSPGQGI 466
AQN +++ F A ++VK+YL T +EW HFG++W+ F ERK +DP +L+PG I
Sbjct: 22 AQNKRVIDFYTGAKMRVKQYLPHYSTQEEWQVHFGSRWETFVERKRAYDPLALLAPGHKI 201
Query: 467 F 467
F
Sbjct: 202 F 204
>BU926889 weakly similar to GP|11120508|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (7%)
Length = 447
Score = 60.1 bits (144), Expect = 2e-09
Identities = 35/71 (49%), Positives = 46/71 (64%), Gaps = 3/71 (4%)
Frame = +1
Query: 1 KLSRNPQILS-QASTDYGHIIHENPFAVLEPTSISDIANLI--NYSNSLPHSFTISPRGQ 57
KL +P+ L +AS DYG+++ E P AV P S SDIA LI +Y+ S+P F I+ RGQ
Sbjct: 238 KLRDDPEALQGRASRDYGNLVREVPLAVFHPASASDIARLIKLSYNGSVP--FKIAARGQ 411
Query: 58 AHSVLGQAMTQ 68
HS GQAM +
Sbjct: 412 GHSTRGQAMAR 444
>TC220851 similar to UP|Q84U27 (Q84U27) Cytokinin dehydrogenase 3 , partial
(9%)
Length = 651
Score = 54.7 bits (130), Expect = 9e-08
Identities = 25/49 (51%), Positives = 32/49 (65%)
Frame = +2
Query: 419 AGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERKAKFDPKRILSPGQGIF 467
A + VK+YL T QEW HFG +W+ F RK+ +DP IL+PGQ IF
Sbjct: 5 AQLGVKQYLPHYNTQQEWRAHFGPQWETFLLRKSVYDPLAILAPGQRIF 151
>AW755511 similar to GP|11120514|gb| cytokinin oxidase {Arabidopsis
thaliana}, partial (12%)
Length = 433
Score = 47.0 bits (110), Expect = 2e-05
Identities = 22/64 (34%), Positives = 37/64 (57%)
Frame = +3
Query: 12 ASTDYGHIIHENPFAVLEPTSISDIANLINYSNSLPHSFTISPRGQAHSVLGQAMTQNGI 71
A D+G + P AV+ P D+A + + + + T++ RG HS+ GQAM +NG+
Sbjct: 198 AGKDFGGMKSAKPLAVIRPAVAGDVARAVKAA-ARKANLTVAARGNGHSINGQAMAENGL 374
Query: 72 VVNM 75
V++M
Sbjct: 375 VLDM 386
>TC204376 similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like protein,
partial (63%)
Length = 1418
Score = 44.7 bits (104), Expect = 9e-05
Identities = 24/75 (32%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
Frame = +1
Query: 133 LSVGGTLSNAGIGGQTFRFGPQISNVLELDVITGQGNIVTCSQEKNSEVFYAVLGGLG-Q 191
+ VGG S G G ++G + NV++ ++ +GN++ + ++F+A+ GG G
Sbjct: 196 VGVGGHFSGGGYGNMLRKYGLSVDNVIDAQIVDVKGNLLN-RKTMGEDLFWAIRGGGGAS 372
Query: 192 FGVITRARILLGPAP 206
FGVI I L P P
Sbjct: 373 FGVILSFTIKLVPVP 417
>TC217973 weakly similar to UP|Q93ZA3 (Q93ZA3) At1g30760/T5I8_22, partial
(55%)
Length = 1432
Score = 38.5 bits (88), Expect = 0.006
Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +1
Query: 133 LSVGGTLSNAGIGGQTFRFGPQISNVLELDVITGQGNIVTCSQEKNSEVFYAVL-GGLGQ 191
L +GG ++ G ++G NVL+ ++ G I+ + ++F+A+ GG G
Sbjct: 31 LGIGGHITGGAYGSMVRKYGLGADNVLDAKIVDANGRILD-RKAMGEDLFWAIRGGGGGS 207
Query: 192 FGVITRARILLGPAP 206
FG++ ++ L P P
Sbjct: 208 FGILLWWKVKLVPVP 252
>BG510985 similar to GP|20279572|gb L-galactono-1 4-lactone dehydrogenase
{Cucumis melo}, partial (20%)
Length = 371
Score = 30.8 bits (68), Expect = 1.3
Identities = 15/55 (27%), Positives = 26/55 (47%)
Frame = +2
Query: 146 GQTFRFGPQISNVLELDVITGQGNIVTCSQEKNSEVFYAVLGGLGQFGVITRARI 200
G R P V+ + ++T + S++K+ E+FY GLG GV+ +
Sbjct: 2 GTGARLPPIDEQVIAMKLVTPAKGTIEISKDKDPELFYLARCGLGGLGVVAEVTL 166
>BG510024 similar to PIR|T10626|T10 reticuline oxidase homolog F21C20.190 -
Arabidopsis thaliana, partial (24%)
Length = 402
Score = 30.8 bits (68), Expect = 1.3
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = +1
Query: 133 LSVGGTLSNAGIGGQTFRFGPQISNVLELDVITGQGNIV 171
+ VGG S G G ++G + NV++ ++ +GN++
Sbjct: 271 VGVGGHFSGGGYGNMLRKYGLSVDNVIDAQIVDVKGNLL 387
>AW306334 weakly similar to GP|23267157|gb| cytokinin dehydrogenase 2
{Hordeum vulgare}, partial (14%)
Length = 409
Score = 30.8 bits (68), Expect = 1.3
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +3
Query: 12 ASTDYGHIIHENPFAVLEPTSISDIANLINY 42
A+ D+G+I H P AVL P ++SDI+ I +
Sbjct: 261 AAKDFGNIHHFPPLAVLYPKTVSDISLTIKH 353
>TC213020 weakly similar to UP|Q8GU71 (Q8GU71) MDR-like ABC transporter,
partial (18%)
Length = 864
Score = 30.0 bits (66), Expect = 2.3
Identities = 15/44 (34%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = +1
Query: 54 PRGQAHSVLGQAMTQNGIVVNMTQLNWYRNGSGIV-VSDCDVKN 96
P G++ +V+GQ+ + V+++ + +Y SG+V V +CD+KN
Sbjct: 22 PAGKSLAVVGQSGSGKSTVISLV-MRFYDPDSGLVLVDECDIKN 150
>TC222486
Length = 711
Score = 29.6 bits (65), Expect = 3.0
Identities = 24/76 (31%), Positives = 33/76 (42%), Gaps = 4/76 (5%)
Frame = -3
Query: 219 RRNDSNGADYVE--GMLLLNKPPLILSF--YPPSDHPRITSLVTQYGITYVLELVKYYDT 274
RR D A + G L+LN+P L+ F YP P T +Y +LEL + +
Sbjct: 682 RRTDPEDAQWCSQFGFLILNQPVLVDKFYSYPELSKPHTT---VRYATKKLLELREQKPS 512
Query: 275 NSQANITEEVDNLVKG 290
N I E + L G
Sbjct: 511 NLSDAIVEHIQILELG 464
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.138 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,103,669
Number of Sequences: 63676
Number of extensions: 301043
Number of successful extensions: 1509
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 1495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1499
length of query: 467
length of database: 12,639,632
effective HSP length: 101
effective length of query: 366
effective length of database: 6,208,356
effective search space: 2272258296
effective search space used: 2272258296
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148775.11