BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148762.7 + phase: 2 /partial
(212 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218065 weakly similar to UP|Q9MAD0 (Q9MAD0) T4P13.10 protein, ... 323 3e-89
BQ629319 210 4e-55
TC216762 similar to UP|Q6NM69 (Q6NM69) At5g46870, partial (73%) 110 6e-25
TC216515 weakly similar to UP|Q9LHY8 (Q9LHY8) ESTs D15336(C0474)... 107 3e-24
TC207715 weakly similar to UP|Q9LHY8 (Q9LHY8) ESTs D15336(C0474)... 107 4e-24
BE660111 105 7e-24
CO979016 54 2e-23
TC218064 70 5e-13
BE020498 42 2e-04
TC206015 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible alpha-... 28 3.1
TC230417 similar to UP|Q9C5V6 (Q9C5V6) Topoisomerase 6 subunit B... 27 5.2
TC211031 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 pr... 27 8.9
>TC218065 weakly similar to UP|Q9MAD0 (Q9MAD0) T4P13.10 protein, partial
(88%)
Length = 1280
Score = 323 bits (829), Expect = 3e-89
Identities = 171/214 (79%), Positives = 190/214 (87%), Gaps = 3/214 (1%)
Frame = +1
Query: 1 SSDYESTAYVTFRDAYALETALLLNGSMILDRCISISRWETYTDDSNNWNNLTPNHEDSI 60
S + TAYVT+RDAYALETALLLNGSMILD+CI ISR+E Y +D +NW+N N E SI
Sbjct: 178 SGENAPTAYVTYRDAYALETALLLNGSMILDQCIFISRYEAYVNDYDNWSNHASNPEYSI 357
Query: 61 TYSQ---MDKFVSSPGEALTMAQQVVKTMVAKGFVLSKDAFVMAKAFDESRSVSSTAANK 117
T SQ MDKFV+SPGEALTMAQ VVKTMVAKG+VL KDAFVMAKAFDES SVSSTAA K
Sbjct: 358 TTSQDVHMDKFVASPGEALTMAQVVVKTMVAKGYVLGKDAFVMAKAFDESHSVSSTAATK 537
Query: 118 VAELSNKIGLTETINSGIETFKSVDEKYHVTDITKSAATVTGTTAIVVATVTGRAAMAAG 177
VAELSNKIGLT+TINSGIETFKSVDEKYHVTD TKSAATVTGTTAIVVA+VTG+AA+AAG
Sbjct: 538 VAELSNKIGLTDTINSGIETFKSVDEKYHVTDFTKSAATVTGTTAIVVASVTGKAAVAAG 717
Query: 178 SAIANSSYFAKGALWVSDMLSRAAKSTADLGHHQ 211
+AIANSSYFAKGALWVSD+L+RAAK+ ADLG ++
Sbjct: 718 TAIANSSYFAKGALWVSDVLTRAAKAAADLGQNE 819
>BQ629319
Length = 447
Score = 210 bits (534), Expect = 4e-55
Identities = 114/140 (81%), Positives = 124/140 (88%), Gaps = 3/140 (2%)
Frame = +3
Query: 45 DSNNWNNLTPNHEDSITYSQ---MDKFVSSPGEALTMAQQVVKTMVAKGFVLSKDAFVMA 101
D +NW++ E SIT SQ MDKFV SPGEALTMAQ VVKTMVAKG+VL KDAFVMA
Sbjct: 27 DYDNWSSHASKPEYSITTSQDVHMDKFVGSPGEALTMAQVVVKTMVAKGYVLGKDAFVMA 206
Query: 102 KAFDESRSVSSTAANKVAELSNKIGLTETINSGIETFKSVDEKYHVTDITKSAATVTGTT 161
KAFDES SVSSTAA+KVAELSNKIGLT+TINSGIETFKSVDEKYHVTD TKSAATVTGTT
Sbjct: 207 KAFDESHSVSSTAASKVAELSNKIGLTDTINSGIETFKSVDEKYHVTDFTKSAATVTGTT 386
Query: 162 AIVVATVTGRAAMAAGSAIA 181
AIVVA+VTG+AA+AAG+AIA
Sbjct: 387 AIVVASVTGKAAVAAGTAIA 446
>TC216762 similar to UP|Q6NM69 (Q6NM69) At5g46870, partial (73%)
Length = 1241
Score = 110 bits (274), Expect = 6e-25
Identities = 74/208 (35%), Positives = 108/208 (51%), Gaps = 7/208 (3%)
Frame = +2
Query: 8 AYVTFRDAYALETALLLNGSMILDRCISISRWETYTDDSNNWNNLTPNHEDSITYSQMDK 67
AY+TF+D+ ETALLL+G+ I+D ++I++ Y L P + +
Sbjct: 398 AYITFKDSQGAETALLLSGATIVDMPVTITQDPDY--------QLPP-----AALASSVR 538
Query: 68 FVSSPG---EALTMAQQVVKTMVAKGFVLSKDAFVMAKAFDESRSVSSTAANKVAELSNK 124
+PG A A+ VV M+AKGF+L KDA AK FDE +SSTA+ KVA K
Sbjct: 539 ETQTPGGADSAFRKAEDVVSGMLAKGFILGKDAVNKAKTFDEKHQLSSTASAKVASFDQK 718
Query: 125 IGLTETINSGI----ETFKSVDEKYHVTDITKSAATVTGTTAIVVATVTGRAAMAAGSAI 180
IGL+E I++G + + VD+K+ V++ TKSA T AGSAI
Sbjct: 719 IGLSEKISAGATVVGDRVREVDQKFQVSEKTKSAFAAAEQT-----------VSNAGSAI 865
Query: 181 ANSSYFAKGALWVSDMLSRAAKSTADLG 208
+ Y GA WV+ S+ +K+ ++G
Sbjct: 866 MKNRYVLTGASWVTGAFSKVSKAAGEVG 949
>TC216515 weakly similar to UP|Q9LHY8 (Q9LHY8) ESTs D15336(C0474), partial
(58%)
Length = 1320
Score = 107 bits (268), Expect = 3e-24
Identities = 73/212 (34%), Positives = 109/212 (50%), Gaps = 15/212 (7%)
Frame = +3
Query: 7 TAYVTFRDAYALETALLLNGSMILDRCISISRWETYTDDSN--------NWNNLTPNHED 58
TAYVTF+D ALE ALLL+G+ I+D+ + I+ E Y + N N+ P+
Sbjct: 342 TAYVTFKDPKALEIALLLSGATIVDQVVKITPAENYVPNREMEEVRVVENAINVAPSENS 521
Query: 59 SITYSQMDKFVSSPGEA---LTMAQQVVKTMVAKGFVLSKDAFVMAKAFDESRSVSSTAA 115
S +++ ++SP L+ AQ V M+AKG + +DA AKAFDE +++ A+
Sbjct: 522 ENVSSNIEEGIASPTNRRIYLSRAQDAVTNMLAKGSAIRQDAVNKAKAFDEKHQLTANAS 701
Query: 116 NKVAELSNKIGLTETINSGI----ETFKSVDEKYHVTDITKSAATVTGTTAIVVATVTGR 171
KV L ++GLTE + GI + KSVD++ V+D T + AI+ A R
Sbjct: 702 AKVISLDKRVGLTEKLTVGIAAVNQKVKSVDQRLQVSDKTMA--------AIIAAE---R 848
Query: 172 AAMAAGSAIANSSYFAKGALWVSDMLSRAAKS 203
GSA+ S Y G W + S+ AK+
Sbjct: 849 KINNTGSAVKTSRYVTAGTAWFNGAFSKVAKA 944
>TC207715 weakly similar to UP|Q9LHY8 (Q9LHY8) ESTs D15336(C0474), partial
(57%)
Length = 975
Score = 107 bits (267), Expect = 4e-24
Identities = 73/210 (34%), Positives = 110/210 (51%), Gaps = 4/210 (1%)
Frame = +3
Query: 2 SDYESTAYVTFRDAYALETALLLNGSMILDRCISISRWETYTDDSNNWNNLTPNHEDSIT 61
S + AYVTF+D +TA+LL GS I D ++I+ E Y L P S
Sbjct: 33 SGHTQVAYVTFKDTQGADTAVLLTGSKIGDLYVTITPVEKY--------QLPPEALPSSP 188
Query: 62 YSQMDKFVSSPGEALTMAQQVVKTMVAKGFVLSKDAFVMAKAFDESRSVSSTAANKVAEL 121
+Q SP +A+ A+ V+ TM+AKGF+L KDA AKAFDE ++S A++ VA +
Sbjct: 189 TNQ------SP-DAVKKAEDVMSTMLAKGFILGKDAINKAKAFDERHQLTSNASSTVASI 347
Query: 122 SNKIGLTETINSGIE----TFKSVDEKYHVTDITKSAATVTGTTAIVVATVTGRAAMAAG 177
KIGL++ ++ G + +DE+Y ++++TKSA + A +AG
Sbjct: 348 DRKIGLSDKLSIGTTIVNGKVREMDERYQLSEMTKSA-----------MAAAEQKASSAG 494
Query: 178 SAIANSSYFAKGALWVSDMLSRAAKSTADL 207
SAI ++ Y GA W S + AK+ D+
Sbjct: 495 SAIMSNPYVKSGASWFSSAFTAIAKAAEDV 584
>BE660111
Length = 590
Score = 105 bits (261), Expect(2) = 7e-24
Identities = 53/97 (54%), Positives = 73/97 (74%), Gaps = 2/97 (2%)
Frame = +2
Query: 1 SSDYESTAYVTFRDAYALETALLLNGSMILDRCISISRWETYTDDSNNWNNLTPNHED-- 58
S DY TAYVTF+DAYA ETA LL+G+ ILD+ + I+RW Y D+ + WN + +HED
Sbjct: 239 SGDYACTAYVTFKDAYAQETACLLSGATILDQRVCITRWGHYEDEFDFWNQPSYSHEDET 418
Query: 59 SITYSQMDKFVSSPGEALTMAQQVVKTMVAKGFVLSK 95
+ T Q ++FVSS GEA+TMAQ+VV+TM++KG+VL +
Sbjct: 419 ASTTPQSNQFVSSAGEAVTMAQEVVRTMLSKGYVLKQ 529
Score = 22.3 bits (46), Expect(2) = 7e-24
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +3
Query: 94 SKDAFVMAKAFDES 107
SKDA AK FDES
Sbjct: 525 SKDALAKAKDFDES 566
>CO979016
Length = 764
Score = 53.5 bits (127), Expect(3) = 2e-23
Identities = 24/43 (55%), Positives = 33/43 (75%)
Frame = -2
Query: 166 ATVTGRAAMAAGSAIANSSYFAKGALWVSDMLSRAAKSTADLG 208
A++TGR+ AA + + NSSYF+KGA+WVS L++ AK ADLG
Sbjct: 295 ASLTGRSVAAAANTMVNSSYFSKGAMWVSGALNQVAKVAADLG 167
Score = 52.4 bits (124), Expect(3) = 2e-23
Identities = 25/38 (65%), Positives = 32/38 (83%)
Frame = -3
Query: 77 TMAQQVVKTMVAKGFVLSKDAFVMAKAFDESRSVSSTA 114
++AQ+VVKTM+AKG+VLSKDA AK FD+S VS+TA
Sbjct: 510 SLAQEVVKTMLAKGYVLSKDALAEAKYFDDSHQVSATA 397
Score = 39.7 bits (91), Expect(3) = 2e-23
Identities = 18/31 (58%), Positives = 23/31 (74%)
Frame = -1
Query: 117 KVAELSNKIGLTETINSGIETFKSVDEKYHV 147
KVAE S +IGLT I+ G+E + VD+KYHV
Sbjct: 392 KVAE*SQRIGLTNKISVGVEAIRYVDQKYHV 300
Score = 30.8 bits (68), Expect = 0.47
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -1
Query: 1 SSDYESTAYVTFRDAYALETAL 22
S DY TAYVTF+DA++ E L
Sbjct: 698 SGDYACTAYVTFKDAHSQENCL 633
>TC218064
Length = 249
Score = 70.5 bits (171), Expect = 5e-13
Identities = 33/42 (78%), Positives = 40/42 (94%)
Frame = +3
Query: 170 GRAAMAAGSAIANSSYFAKGALWVSDMLSRAAKSTADLGHHQ 211
G+AA+AAG+AIANSSYFAKGALWVSDML+RAAK+ ADLG ++
Sbjct: 6 GKAAVAAGTAIANSSYFAKGALWVSDMLTRAAKTAADLGQNE 131
>BE020498
Length = 407
Score = 42.0 bits (97), Expect = 2e-04
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = +1
Query: 1 SSDYESTAYVTFRDAYALETALLLN 25
S + STAYVT+RDAYALETALLLN
Sbjct: 82 SGENASTAYVTYRDAYALETALLLN 156
Score = 40.4 bits (93), Expect = 6e-04
Identities = 16/26 (61%), Positives = 22/26 (84%)
Frame = +3
Query: 26 GSMILDRCISISRWETYTDDSNNWNN 51
GSMILD+CI ISR+E Y +D +NW++
Sbjct: 312 GSMILDQCIFISRYEAYVNDYDNWSS 389
>TC206015 similar to UP|Q9AXU5 (Q9AXU5) Pathogen-inducible alpha-dioxygenase,
partial (61%)
Length = 1494
Score = 28.1 bits (61), Expect = 3.1
Identities = 32/115 (27%), Positives = 52/115 (44%), Gaps = 2/115 (1%)
Frame = +3
Query: 34 ISISRWETYTDDSNNWNNLTPNHEDSITYSQMDKFVSSPGEALTMAQQVVKTMVAKGFVL 93
I IS+WE TDD L + D + ++D V MA++ + KGF +
Sbjct: 774 IPISKWEDLTDDKEAIQVLEEVYGDDV--EELDVLVG------LMAEKKI-----KGFAI 914
Query: 94 SKDAFVMAKAFDESRSVSST--AANKVAELSNKIGLTETINSGIETFKSVDEKYH 146
S+ AFV+ R + +N E K GL E +N+ E+ K V ++++
Sbjct: 915 SETAFVIFLLMATRRLEADRFFTSNFNEETYTKKGL-EWVNT-TESLKDVIDRHY 1073
>TC230417 similar to UP|Q9C5V6 (Q9C5V6) Topoisomerase 6 subunit B, partial
(34%)
Length = 937
Score = 27.3 bits (59), Expect = 5.2
Identities = 21/79 (26%), Positives = 39/79 (48%), Gaps = 8/79 (10%)
Frame = +1
Query: 87 VAKGFVLSKDAFVMAKAFDESRSVSSTAANK--------VAELSNKIGLTETINSGIETF 138
V +G + + A + F++ V +T A+K + ++ +KIG+ +I S F
Sbjct: 97 VKQGLNIFRFANRIPLLFEQGADVVTTTAHKRINWSSYKINQMQDKIGVFVSIVSTKIPF 276
Query: 139 KSVDEKYHVTDITKSAATV 157
K ++Y DIT+ A+ V
Sbjct: 277 KGTGKEYIGDDITEIASAV 333
>TC211031 weakly similar to UP|Q8L685 (Q8L685) Pherophorin-dz1 protein
precursor, partial (11%)
Length = 678
Score = 26.6 bits (57), Expect = 8.9
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +2
Query: 149 DITKSAATVTGTTAIVVATVTGRAAMAAGSAIANSSYFA 187
D T ++ TVT T+ V T+T SA ++Y A
Sbjct: 185 DTTSTSTTVTTITSTTVTTITSTTVTTITSATLTATYTA 301
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.312 0.122 0.333
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,830,785
Number of Sequences: 63676
Number of extensions: 62038
Number of successful extensions: 316
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 305
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 307
length of query: 212
length of database: 12,639,632
effective HSP length: 93
effective length of query: 119
effective length of database: 6,717,764
effective search space: 799413916
effective search space used: 799413916
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC148762.7