BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148755.9 + phase: 0
(527 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228155 similar to UP|HEMZ_CUCSA (P42044) Ferrochelatase II, ch... 215 4e-56
TC228154 similar to UP|HMZ1_ARATH (P42043) Ferrochelatase I, chl... 208 5e-54
BE440729 similar to GP|12082085|db ferrochelatase {Cucumis sativ... 204 8e-53
TC233407 homologue to UP|Q949G2 (Q949G2) Ferrochelatase , parti... 191 9e-49
TC234073 homologue to UP|O64391 (O64391) Ferrochelatase , parti... 55 1e-07
BI968443 similar to GP|12082085|dbj ferrochelatase {Cucumis sati... 48 1e-05
TC221276 homologue to UP|Q9ZSK8 (Q9ZSK8) Ferrochelatase (Fragmen... 47 2e-05
TC220944 similar to UP|HEMZ_CUCSA (P42044) Ferrochelatase II, ch... 45 1e-04
CF920449 38 0.010
CF923119 38 0.013
TC205631 weakly similar to UP|CBR_DUNBA (P27516) Carotene biosyn... 37 0.022
TC225114 similar to UP|Q6NKS4 (Q6NKS4) At5g47110, partial (58%) 36 0.037
TC225125 similar to UP|Q6NKS4 (Q6NKS4) At5g47110, partial (58%) 34 0.14
TC204939 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F2... 33 0.41
TC204937 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F2... 33 0.41
TC204942 homologue to UP|Q6W5F3 (Q6W5F3) Microtubule-associated ... 33 0.41
TC207501 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F2... 32 0.53
BQ297416 32 0.91
TC215536 similar to UP|SNA2_ARATH (Q9SPE6) Alpha-soluble NSF att... 31 1.2
BI321770 weakly similar to GP|9796478|gb|A serine threonine kina... 31 1.6
>TC228155 similar to UP|HEMZ_CUCSA (P42044) Ferrochelatase II, chloroplast
precursor (Protoheme ferro-lyase) (Heme synthetase) ,
partial (33%)
Length = 637
Score = 215 bits (547), Expect = 4e-56
Identities = 107/155 (69%), Positives = 131/155 (84%), Gaps = 3/155 (1%)
Frame = +2
Query: 210 GMRYWHPFTEEAIELIKRDGITKLVVLPLYPQFSISTSGSSLRLLESIFREDEYLVNMQH 269
GMRYW+PFTEEAI+ IKRD IT+LVVLPLYPQFSIST+GSS+R+LE IFRED YL N+
Sbjct: 2 GMRYWYPFTEEAIQQIKRDRITRLVVLPLYPQFSISTTGSSIRVLEHIFREDAYLSNLPV 181
Query: 270 TVIPSWYQREGYIKAMANLIEKELKGFDLPEKVMIFFSAHGVPVAYVEEAGDPYKAEMEE 329
++I SWYQREGYIK+MANLI+KEL+ F P++VMIFFSAHGVPV+YVEEAGDPY+ +MEE
Sbjct: 182 SIINSWYQREGYIKSMANLIQKELQSFSEPKEVMIFFSAHGVPVSYVEEAGDPYRDQMEE 361
Query: 330 CVDLIMEELEKRKITN---AYTLAYQSRVGPVEWL 361
C+ LIM+EL+ R I+N +YTLA + VEW+
Sbjct: 362 CIFLIMQELKARGISNFLRSYTLA--G*ISRVEWV 460
Score = 69.3 bits (168), Expect = 4e-12
Identities = 29/47 (61%), Positives = 41/47 (86%)
Frame = +1
Query: 342 KITNAYTLAYQSRVGPVEWLKPYTDETIIELGKKGVKSLLAVPISFV 388
+I ++ + +QSRVGPV+WLKPYTDE ++ELG+KGVKSLLAVP+ ++
Sbjct: 415 EIIHSCWMNFQSRVGPVQWLKPYTDEVLVELGQKGVKSLLAVPVRYI 555
>TC228154 similar to UP|HMZ1_ARATH (P42043) Ferrochelatase I,
chloroplast/mitochondrial precursor (Protoheme
ferro-lyase) (Heme synthetase) , partial (35%)
Length = 818
Score = 208 bits (529), Expect = 5e-54
Identities = 100/141 (70%), Positives = 119/141 (83%)
Frame = +1
Query: 304 IFFSAHGVPVAYVEEAGDPYKAEMEECVDLIMEELEKRKITNAYTLAYQSRVGPVEWLKP 363
I SAH PV+YVE+ DPY+ +MEEC+ IM+E + R I+N LAYQSRVGPV+WLKP
Sbjct: 1 IXXSAHXXPVSYVEKXXDPYRDQMEECIXXIMQEXKARGISNEXXLAYQSRVGPVQWLKP 180
Query: 364 YTDETIIELGKKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIENWGRVPALGCE 423
YTDE ++ELG+KGVKSLLAVP+SFVSEHIETLEEID+EYKELALESGI+NW RVPALG
Sbjct: 181 YTDEVLVELGQKGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIKNWARVPALGVT 360
Query: 424 PTFISDLADAVIESLPYVGAM 444
P+FI+DLADAVIE+LP A+
Sbjct: 361 PSFITDLADAVIEALPSATAI 423
>BE440729 similar to GP|12082085|db ferrochelatase {Cucumis sativus}, partial
(21%)
Length = 466
Score = 204 bits (519), Expect = 8e-53
Identities = 97/114 (85%), Positives = 103/114 (90%)
Frame = +3
Query: 414 WGRVPALGCEPTFISDLADAVIESLPYVGAMAVSNLEARQSLVPLGSVEELLAAYDSQRR 473
WGRVPALGCEPTFISDLADAVIESLPYVGAM S+LEA+QSLVPLGSVEELLAAYDSQRR
Sbjct: 6 WGRVPALGCEPTFISDLADAVIESLPYVGAMTASDLEAQQSLVPLGSVEELLAAYDSQRR 185
Query: 474 ELPPPILVWEWGWTKSAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGILPLFR 527
ELPPP++VWEWGWTKSAETWNGR AM+AVLLLLF E T +G LHQ GI PL R
Sbjct: 186 ELPPPVIVWEWGWTKSAETWNGRVAMLAVLLLLFFEFTADKGLLHQMGIWPLLR 347
>TC233407 homologue to UP|Q949G2 (Q949G2) Ferrochelatase , partial (19%)
Length = 421
Score = 191 bits (484), Expect = 9e-49
Identities = 91/94 (96%), Positives = 94/94 (99%)
Frame = +3
Query: 434 VIESLPYVGAMAVSNLEARQSLVPLGSVEELLAAYDSQRRELPPPILVWEWGWTKSAETW 493
VIESLPYVGAMAVSNLEARQSLVPLGSVEELLAAYDSQRRELPPP++VWEWGWTKSAETW
Sbjct: 3 VIESLPYVGAMAVSNLEARQSLVPLGSVEELLAAYDSQRRELPPPVIVWEWGWTKSAETW 182
Query: 494 NGRAAMIAVLLLLFLEVTTGEGFLHQWGILPLFR 527
NGRAAM+AVLLLLFLEVTTGEGFLHQWGILPLFR
Sbjct: 183 NGRAAMLAVLLLLFLEVTTGEGFLHQWGILPLFR 284
>TC234073 homologue to UP|O64391 (O64391) Ferrochelatase , partial (4%)
Length = 400
Score = 54.7 bits (130), Expect = 1e-07
Identities = 20/23 (86%), Positives = 23/23 (99%)
Frame = +1
Query: 466 AAYDSQRRELPPPILVWEWGWTK 488
AAYDSQRRELPPP++VWEWGWT+
Sbjct: 1 AAYDSQRRELPPPVIVWEWGWTR 69
>BI968443 similar to GP|12082085|dbj ferrochelatase {Cucumis sativus},
partial (3%)
Length = 136
Score = 47.8 bits (112), Expect = 1e-05
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = -3
Query: 488 KSAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGILPLFR 527
KSAETWNGR M+AV+++LF +G +HQ GI P R
Sbjct: 134 KSAETWNGRVXMLAVVVVLFFGFXADKGLVHQMGIWPSVR 15
>TC221276 homologue to UP|Q9ZSK8 (Q9ZSK8) Ferrochelatase (Fragment), partial
(14%)
Length = 581
Score = 47.0 bits (110), Expect = 2e-05
Identities = 24/46 (52%), Positives = 34/46 (73%), Gaps = 3/46 (6%)
Frame = +1
Query: 92 PVEALVTSTTQDVS--DTPL-IGDDKIGVLLLNLGGPETLDDVQPF 134
P V ++ DV+ ++P + ++K+GVLLLNLGGPETL+DVQPF
Sbjct: 442 PASYSVETSAYDVASLESPSHVAEEKVGVLLLNLGGPETLNDVQPF 579
>TC220944 similar to UP|HEMZ_CUCSA (P42044) Ferrochelatase II, chloroplast
precursor (Protoheme ferro-lyase) (Heme synthetase) ,
partial (6%)
Length = 444
Score = 44.7 bits (104), Expect = 1e-04
Identities = 27/60 (45%), Positives = 40/60 (66%), Gaps = 4/60 (6%)
Frame = +2
Query: 78 QPLVSKQSLNRHLL-PVEALVTSTTQDVS--DTPL-IGDDKIGVLLLNLGGPETLDDVQP 133
+PL S R+L+ P + ++ DV+ ++P + ++K+GVLLLNLGGPETL DVQP
Sbjct: 263 RPLCVNPSGRRNLVGPAFYSLETSAYDVAALESPSRVAEEKVGVLLLNLGGPETLSDVQP 442
>CF920449
Length = 465
Score = 38.1 bits (87), Expect = 0.010
Identities = 17/22 (77%), Positives = 20/22 (90%)
Frame = -2
Query: 418 PALGCEPTFISDLADAVIESLP 439
PALG P+FI+DLADAVIE+LP
Sbjct: 464 PALGVTPSFITDLADAVIEALP 399
>CF923119
Length = 224
Score = 37.7 bits (86), Expect = 0.013
Identities = 22/77 (28%), Positives = 40/77 (51%)
Frame = -1
Query: 206 NVYVGMRYWHPFTEEAIELIKRDGITKLVVLPLYPQFSISTSGSSLRLLESIFREDEYLV 265
++Y+G+++ PF +A++ + DGI + L L P +S + S + + E E L
Sbjct: 224 HMYLGLKHIEPFMXDAVKDMHNDGIQDAIALVLAPHYSTFSVKSYVGRAQ---EEAEKLG 54
Query: 266 NMQHTVIPSWYQREGYI 282
N+ I SWY+ +I
Sbjct: 53 NLTIHGIDSWYKEPKFI 3
>TC205631 weakly similar to UP|CBR_DUNBA (P27516) Carotene
biosynthesis-related protein CBR, chloroplast precursor,
partial (17%)
Length = 1088
Score = 37.0 bits (84), Expect = 0.022
Identities = 20/40 (50%), Positives = 25/40 (62%)
Frame = +1
Query: 484 WGWTKSAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGIL 523
+G+T E NGRAA+I LLLL E+ TG+G L G L
Sbjct: 727 FGFTGKNEIINGRAAVIGFLLLLDFELLTGKGLLKGTGFL 846
>TC225114 similar to UP|Q6NKS4 (Q6NKS4) At5g47110, partial (58%)
Length = 1210
Score = 36.2 bits (82), Expect = 0.037
Identities = 19/48 (39%), Positives = 25/48 (51%), Gaps = 5/48 (10%)
Frame = +2
Query: 479 ILVWEWGWTK-----SAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWG 521
I+ W W W K AE NGRAAM+ + F++ TG G + Q G
Sbjct: 626 IIPW-WAWMKRFHLPEAELLNGRAAMVGFFMAYFVDSLTGVGLVDQMG 766
>TC225125 similar to UP|Q6NKS4 (Q6NKS4) At5g47110, partial (58%)
Length = 1193
Score = 34.3 bits (77), Expect = 0.14
Identities = 19/46 (41%), Positives = 24/46 (51%), Gaps = 5/46 (10%)
Frame = +2
Query: 479 ILVWEWGWTK-----SAETWNGRAAMIAVLLLLFLEVTTGEGFLHQ 519
I+ W W W K AE NGRAAMI + F++ TG G + Q
Sbjct: 653 IVPW-WAWMKRFHLPEAELLNGRAAMIGFFMAYFVDSLTGVGLVDQ 787
>TC204939 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F28A23_50
{Arabidopsis thaliana;} , partial (53%)
Length = 727
Score = 32.7 bits (73), Expect = 0.41
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 489 SAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGI 522
S + W GR AM+ + + +E+ TG+G L +G+
Sbjct: 339 SLDVWLGRLAMVGFAVAITVEIATGKGLLENFGL 440
>TC204937 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F28A23_50
{Arabidopsis thaliana;} , partial (53%)
Length = 514
Score = 32.7 bits (73), Expect = 0.41
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = +3
Query: 489 SAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGI 522
S + W GR AM+ + + +E+ TG+G L +G+
Sbjct: 159 SLDVWLGRLAMVGFAVAITVEIATGKGLLENFGL 260
>TC204942 homologue to UP|Q6W5F3 (Q6W5F3) Microtubule-associated protein 1
light chain 3, partial (84%)
Length = 1000
Score = 32.7 bits (73), Expect = 0.41
Identities = 12/34 (35%), Positives = 21/34 (61%)
Frame = -2
Query: 489 SAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGI 522
S + W GR AM+ + + +E+ TG+G L +G+
Sbjct: 348 SLDVWLGRLAMVGFAVAITVEIATGKGLLENFGL 247
>TC207501 similar to GB|AAN38679.1|23507751|BT000842 At4g34190/F28A23_50
{Arabidopsis thaliana;} , partial (52%)
Length = 724
Score = 32.3 bits (72), Expect = 0.53
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +3
Query: 491 ETWNGRAAMIAVLLLLFLEVTTGEGFLHQWGI 522
+ W GR AM+ + + +E++TG+G L +G+
Sbjct: 330 DVWLGRLAMVGFAVAITVEISTGKGLLENFGV 425
>BQ297416
Length = 422
Score = 31.6 bits (70), Expect = 0.91
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +3
Query: 483 EWGWTKSAETWNGRAAMIAVLLLLFLEVTTGEGFLH 518
E G+++ +E NGR A++ + LL +E+ TG+G ++
Sbjct: 279 ELGFSQYSERLNGRIAILGLTALLLVELATGKGVIN 386
>TC215536 similar to UP|SNA2_ARATH (Q9SPE6) Alpha-soluble NSF attachment
protein 2 (Alpha-SNAP2) (N-ethylmaleimide-sensitive
factor attachment protein, alpha 2), complete
Length = 1264
Score = 31.2 bits (69), Expect = 1.2
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 479 ILVWEWGWTKSAETWNGRAAMIAVLLLLFLEVTTGEGFLH 518
I +W W WT T G + + +L LLFL+ T + +H
Sbjct: 60 IWIWIWIWTLVVNTAFGPSLRLRILFLLFLQRTAFDQSIH 179
>BI321770 weakly similar to GP|9796478|gb|A serine threonine kinase homolog
COK-4 {Phaseolus vulgaris}, partial (11%)
Length = 407
Score = 30.8 bits (68), Expect = 1.6
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = +3
Query: 476 PPPILVWEWGWTKSAETWNGRAAMIAVLLLLFLEVTTGEGFLHQWG 521
PPP+L WEW W TW L+L LEV T + + WG
Sbjct: 102 PPPLLTWEWNWNL---TWKR-------LILEMLEV*T-KWATNNWG 206
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,945,113
Number of Sequences: 63676
Number of extensions: 347217
Number of successful extensions: 1901
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 1892
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1900
length of query: 527
length of database: 12,639,632
effective HSP length: 102
effective length of query: 425
effective length of database: 6,144,680
effective search space: 2611489000
effective search space used: 2611489000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148755.9