BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148755.4 + phase: 1 /partial
(441 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI425075 237 9e-63
TC212387 weakly similar to UP|Q9ZW89 (Q9ZW89) F5A8.9 protein, pa... 51 1e-06
TC227392 41 0.001
BE474559 40 0.002
TC230475 40 0.003
TC227743 similar to PIR|E86326|E86326 protein F18O14.3 [imported... 39 0.006
TC206224 similar to PIR|E86326|E86326 protein F18O14.3 [imported... 39 0.006
BM085273 38 0.008
TC216036 similar to GB|AAP13393.1|30023720|BT006285 At4g27470 {A... 38 0.008
TC221337 similar to UP|Q9SD57 (Q9SD57) RNA-binding protein-like ... 38 0.010
TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, pa... 37 0.014
TC218509 similar to UP|Q6GKW2 (Q6GKW2) At1g24580, partial (41%) 37 0.014
BF423589 similar to PIR|T51853|T518 RING-H2 finger protein RHF1a... 37 0.014
TC228034 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {A... 37 0.018
TC219821 weakly similar to UP|PJA1_MOUSE (O55176) Ubiquitin prot... 37 0.018
TC228035 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {A... 37 0.018
TC217772 similar to GB|AAM51588.1|21436021|AY116954 AT5g19430/F7... 37 0.023
TC210454 weakly similar to GB|AAP68217.1|31711722|BT008778 At3g0... 37 0.023
TC228188 similar to GB|AAP31929.1|30387527|BT006585 At3g58030 {A... 37 0.023
TC232492 weakly similar to UP|Q8VYW5 (Q8VYW5) AT5g45290/K9E15_7,... 37 0.023
>BI425075
Length = 408
Score = 237 bits (604), Expect = 9e-63
Identities = 107/134 (79%), Positives = 115/134 (84%)
Frame = +1
Query: 225 QSDGPDHHCSQCVTVVGGFQCDPSTIHLQCQECGGMMPSRTGLGVPQYCLGCDRPFCGAY 284
QSDG H C QCVT V GF+C T+HLQCQ CGGMMPSRTG G+PQYC GCDR FCGAY
Sbjct: 1 QSDGTYHQCQQCVTEVAGFRCKYDTVHLQCQACGGMMPSRTGFGIPQYCSGCDRSFCGAY 180
Query: 285 WSALGVTGSGSYPVCSPDTLRPISEHTISGIPLVAHEKNLHEQNITESCIRQMGRTLQDV 344
W ALGVTG+GSYPVCS DTLRPIS+H+IS IPL+AHEKNLHEQNIT+SCIRQMGRTL DV
Sbjct: 181 WHALGVTGNGSYPVCSQDTLRPISDHSISRIPLLAHEKNLHEQNITDSCIRQMGRTLPDV 360
Query: 345 ISEWIAKLNNREID 358
ISEWIAK NREID
Sbjct: 361 ISEWIAKFENREID 402
>TC212387 weakly similar to UP|Q9ZW89 (Q9ZW89) F5A8.9 protein, partial (16%)
Length = 1107
Score = 50.8 bits (120), Expect = 1e-06
Identities = 39/145 (26%), Positives = 56/145 (37%), Gaps = 13/145 (8%)
Frame = +1
Query: 35 IVRNSDLCSATLKNKSSNTILVDGAEVGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIV-S 93
I++NS+ +A L N+S I G D IKD S I F+ + +
Sbjct: 292 ILQNSEGPNAGLSNQSRTII------GGSNDIEQIKDSSHISKPRNSTSFIEDPLPVPQT 453
Query: 94 SPEICQTLLKICVDVDHA------------KCSICLNIWHDVVTVAPCLHNFCNGCFSEW 141
S ++C + + D D A C ICL + + PC H FC C W
Sbjct: 454 SIDLCSSDDEKFTDGDQADNVAGLPTSTEMSCVICLTEFSSTRGILPCGHRFCFPCIQNW 633
Query: 142 LRRSQEKRSTVLCPQCRAVVQFVGK 166
+ R T CP C+A + K
Sbjct: 634 ADHTTSMRKTSTCPLCKASFMMIKK 708
>TC227392
Length = 1464
Score = 41.2 bits (95), Expect = 0.001
Identities = 23/58 (39%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
Frame = +1
Query: 106 VDVDHAKCSICLNIWH--DVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVV 161
V + ++C ICL +H + V PC HNF C EWLR + V CP+CR V
Sbjct: 640 VPTNCSECLICLEEFHVGNQVRGLPCAHNFHVECIDEWLRLN------VNCPRCRCSV 795
>BE474559
Length = 414
Score = 40.4 bits (93), Expect = 0.002
Identities = 23/58 (39%), Positives = 28/58 (47%)
Frame = +3
Query: 101 LLKICVDVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
LL+I DV +C ICL I TV CLH FC C + +R + CP CR
Sbjct: 63 LLEIRKDV---QCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNE-----CPACR 212
>TC230475
Length = 528
Score = 39.7 bits (91), Expect = 0.003
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = +3
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
C+IC H + + C H FC C SEW R + CP CRA+V+
Sbjct: 297 CAICQEKMHAPILLR-CKHIFCEDCVSEWFERER------TCPLCRALVK 425
>TC227743 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(73%)
Length = 1212
Score = 38.5 bits (88), Expect = 0.006
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +2
Query: 107 DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
D + +C+IC + D + + C H FC C +WL + R CP C+A+V+
Sbjct: 200 DAANFECNICFELAQDPI-ITLCGHLFCWPCLYKWLHFHSQSRE---CPVCKALVE 355
>TC206224 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(43%)
Length = 1080
Score = 38.5 bits (88), Expect = 0.006
Identities = 19/56 (33%), Positives = 27/56 (47%)
Frame = +1
Query: 107 DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
D +C+IC ++ D V + C H FC C WL + CP C+A+VQ
Sbjct: 298 DAGDFECNICFDLAQDPV-ITLCGHLFCWPCLYRWLHHHSHSQE---CPVCKALVQ 453
>BM085273
Length = 424
Score = 38.1 bits (87), Expect = 0.008
Identities = 20/76 (26%), Positives = 36/76 (47%)
Frame = +2
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFLRT 172
C ICL + + +V + C H+ C C+ +W RSQ CP CR ++ V +
Sbjct: 2 CGICLEM-NSMVVLPNCNHSMCMKCYEDWHARSQS------CPFCRDSLKRVNSDDLWIC 160
Query: 173 IAEDMVKADSSLRRSH 188
I+ + +S+ + +
Sbjct: 161 ISSSEINDLASINKEN 208
>TC216036 similar to GB|AAP13393.1|30023720|BT006285 At4g27470 {Arabidopsis
thaliana;} , partial (23%)
Length = 1061
Score = 38.1 bits (87), Expect = 0.008
Identities = 24/77 (31%), Positives = 35/77 (45%)
Frame = +3
Query: 81 KEGFVSYKFQIVSSPEICQTLLKICVDVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSE 140
+E +VS + + + SP L C D C+ICL+ H+ V V C H +C C +
Sbjct: 201 EEHYVSQELKTIPSPMTVTENLDGCFD-----CNICLDFAHEPV-VTLCGHLYCWPCIYK 362
Query: 141 WLRRSQEKRSTVLCPQC 157
WL + PQC
Sbjct: 363 WLHVQSASLAPDEHPQC 413
>TC221337 similar to UP|Q9SD57 (Q9SD57) RNA-binding protein-like protein,
partial (77%)
Length = 1151
Score = 37.7 bits (86), Expect = 0.010
Identities = 21/77 (27%), Positives = 36/77 (46%)
Frame = +1
Query: 112 KCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFLR 171
+C ICL I + V + C H+ C C+ +W RSQ CP CR ++ V +
Sbjct: 751 ECGICLEI-NSKVVLPNCNHSMCMKCYEDWHARSQS------CPFCRDSLKRVNTDDLWI 909
Query: 172 TIAEDMVKADSSLRRSH 188
I+ + +S+ + +
Sbjct: 910 YISSSEINDLASINKEN 960
>TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, partial (8%)
Length = 1326
Score = 37.4 bits (85), Expect = 0.014
Identities = 18/50 (36%), Positives = 25/50 (50%)
Frame = +3
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQ 162
C+IC + ++ C H FC C SEW R + CP CRA+V+
Sbjct: 828 CAICQEKMQAPILLS-CKHMFCEECVSEWFERER------TCPLCRALVK 956
>TC218509 similar to UP|Q6GKW2 (Q6GKW2) At1g24580, partial (41%)
Length = 671
Score = 37.4 bits (85), Expect = 0.014
Identities = 34/130 (26%), Positives = 56/130 (42%), Gaps = 4/130 (3%)
Frame = +2
Query: 35 IVRNSDLCSATLKN--KSSNTILVDGAEVGKGDTVVIKDRSEIIPGPAKEGFVSYKFQIV 92
+V N+ L A LKN +S +++ G+ G + D S + YK +
Sbjct: 164 LVINTVLSVAVLKNMFRSMLQVVLGGSAAANGSNIE-HDESSSSSWERRVSITQYK-SLC 337
Query: 93 SSPEICQTLLKICVDVDHAKCSICLNIW--HDVVTVAPCLHNFCNGCFSEWLRRSQEKRS 150
S +I T + + +C +CL + + V+ PC H F GC +W K +
Sbjct: 338 HSHDIGGTSMAM------VECCVCLCRFEANQEVSELPCKHYFHRGCLDKWF---DNKHT 490
Query: 151 TVLCPQCRAV 160
T CP CR++
Sbjct: 491 T--CPLCRSI 514
>BF423589 similar to PIR|T51853|T518 RING-H2 finger protein RHF1a [imported]
- Arabidopsis thaliana (fragment), partial (21%)
Length = 413
Score = 37.4 bits (85), Expect = 0.014
Identities = 21/53 (39%), Positives = 25/53 (46%), Gaps = 2/53 (3%)
Frame = +3
Query: 107 DVDHAKCSICLNIW--HDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQC 157
D CSICL + HD TV C H + C EW +RS+E CP C
Sbjct: 159 DSSEDSCSICLEPFSVHDPSTVTCCKHEYHLHCIIEWSQRSKE------CPIC 299
>TC228034 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;} , partial (67%)
Length = 947
Score = 37.0 bits (84), Expect = 0.018
Identities = 19/52 (36%), Positives = 24/52 (45%), Gaps = 2/52 (3%)
Frame = +3
Query: 109 DHAKCSICLNIWH--DVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
D CS+CL + DV+ PCLH F C WLR+ CP C+
Sbjct: 573 DDLTCSVCLEQVNVGDVLRSLPCLHQFHANCIDPWLRQQG------TCPVCK 710
>TC219821 weakly similar to UP|PJA1_MOUSE (O55176) Ubiquitin protein ligase
Praja1 , partial (4%)
Length = 1135
Score = 37.0 bits (84), Expect = 0.018
Identities = 30/115 (26%), Positives = 57/115 (49%), Gaps = 7/115 (6%)
Frame = +3
Query: 108 VDHAKCSICLNIWHD--VVTVAP-CLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFV 164
++H +C ICL + + V+ + P C H F C WLR K+ST CP CR ++
Sbjct: 549 LEHTQCVICLADYREREVLRIMPKCGHTFHLSCIDIWLR----KQST--CPVCRLPLKNS 710
Query: 165 GKNHFLR----TIAEDMVKADSSLRRSHDEVALLDTYASVRSNLVIGSGKKSRKR 215
+ +R T+++ + ++ +S R E + T + ++L SG++ ++
Sbjct: 711 SETKHVRPVTFTMSQSLDESHTSDRNDDIERYVEPTPTAASNSLQPTSGEQEARQ 875
>TC228035 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;} , partial (19%)
Length = 582
Score = 37.0 bits (84), Expect = 0.018
Identities = 19/52 (36%), Positives = 24/52 (45%), Gaps = 2/52 (3%)
Frame = +3
Query: 109 DHAKCSICLNIWH--DVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCR 158
D CS+CL + DV+ PCLH F C WLR+ CP C+
Sbjct: 135 DDLTCSVCLEQVNVGDVLRSLPCLHQFHANCIDPWLRQQG------TCPVCK 272
>TC217772 similar to GB|AAM51588.1|21436021|AY116954 AT5g19430/F7K24_180
{Arabidopsis thaliana;} , partial (61%)
Length = 1136
Score = 36.6 bits (83), Expect = 0.023
Identities = 20/72 (27%), Positives = 32/72 (43%), Gaps = 2/72 (2%)
Frame = +2
Query: 113 CSICLN--IWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFL 170
C+ICL+ + + V C H +C C W R V CPQC+ +F+ + L
Sbjct: 311 CAICLDKIVLQETALVKGCEHAYCVTCILHWATY----REKVTCPQCKHPFEFLNVHRSL 478
Query: 171 RTIAEDMVKADS 182
+D + +S
Sbjct: 479 DGSIQDYMFEES 514
>TC210454 weakly similar to GB|AAP68217.1|31711722|BT008778 At3g05250
{Arabidopsis thaliana;} , partial (25%)
Length = 449
Score = 36.6 bits (83), Expect = 0.023
Identities = 21/84 (25%), Positives = 35/84 (41%), Gaps = 8/84 (9%)
Frame = +1
Query: 113 CSICLNIWHDVVTVAPCLHNFCNGCFSEWLR----RSQEKRSTVLCPQCR----AVVQFV 164
C ICL + + + C H FC C W + + + S+V CP C+ +++ V
Sbjct: 70 CPICLGSFLQLSYLDKCFHKFCFNCILRWTKVVASKHRSPPSSVKCPLCKTENFSIIYVV 249
Query: 165 GKNHFLRTIAEDMVKADSSLRRSH 188
+ F R + L R+H
Sbjct: 250 DGSCFQRHYVNQDFENSFILSRAH 321
>TC228188 similar to GB|AAP31929.1|30387527|BT006585 At3g58030 {Arabidopsis
thaliana;} , partial (27%)
Length = 877
Score = 36.6 bits (83), Expect = 0.023
Identities = 20/55 (36%), Positives = 26/55 (46%)
Frame = +1
Query: 107 DVDHAKCSICLNIWHDVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVV 161
D D C+ICL++ D V V C H FC C WL + + CP C+ V
Sbjct: 223 DGDFFDCNICLDLARDPV-VTCCGHLFCWPCLYRWLHLHSDAKE---CPVCKGEV 375
>TC232492 weakly similar to UP|Q8VYW5 (Q8VYW5) AT5g45290/K9E15_7, partial
(6%)
Length = 582
Score = 36.6 bits (83), Expect = 0.023
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
Frame = +1
Query: 113 CSICLNIWH--DVVTVAPCLHNFCNGCFSEWLRRSQEKRSTVLCPQCRAVVQFVGKNHFL 170
C +CL+ D + V PC H F C ++WL+ + CP CR + K+H
Sbjct: 187 CCVCLSRLKAKDEIRVLPCSHKFHKICVNKWLKGRHK-----TCPLCRFSMGAEEKSHRA 351
Query: 171 RTIAEDMV 178
E+M+
Sbjct: 352 EMFTEEML 375
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.134 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,818,481
Number of Sequences: 63676
Number of extensions: 443862
Number of successful extensions: 2331
Number of sequences better than 10.0: 146
Number of HSP's better than 10.0 without gapping: 2316
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2329
length of query: 441
length of database: 12,639,632
effective HSP length: 100
effective length of query: 341
effective length of database: 6,272,032
effective search space: 2138762912
effective search space used: 2138762912
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC148755.4