BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148654.3 - phase: 0 /pseudo
(719 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218193 similar to GB|AAO64894.1|29029030|BT005959 At5g08720 {A... 425 e-124
TC218194 similar to GB|AAO64894.1|29029030|BT005959 At5g08720 {A... 320 2e-87
CA938843 139 4e-33
BQ273609 65 8e-11
TC230818 similar to UP|Q9LDH9 (Q9LDH9) Gb|AAF34826.1 (MJM20.1 pr... 38 0.018
TC212585 36 0.068
TC208914 31 1.7
TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, pa... 31 2.2
TC227543 30 2.9
TC219861 similar to UP|O24041 (O24041) Laccase , partial (14%) 30 4.9
TC207159 similar to GB|AAR18374.1|38350523|AY444865 nucleobase-a... 30 4.9
TC208150 similar to UP|Q6X182 (Q6X182) Mal d 1-associated protei... 29 6.4
TC224859 weakly similar to UP|Q7TP79 (Q7TP79) Aa2-245, partial (3%) 29 6.4
TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family prote... 29 8.3
BI969600 similar to GP|10177224|dbj ribitol dehydrogenase-like {... 29 8.3
TC214632 homologue to UP|Q9LKH8 (Q9LKH8) NADPH-protochlorophylli... 29 8.3
TC214136 similar to UP|Q84L12 (Q84L12) Peptide methionine sulfox... 29 8.3
TC207258 similar to UP|IF2C_PHAVU (P57997) Translation initiatio... 29 8.3
>TC218193 similar to GB|AAO64894.1|29029030|BT005959 At5g08720 {Arabidopsis
thaliana;} , partial (33%)
Length = 1055
Score = 425 bits (1092), Expect(2) = e-124
Identities = 207/247 (83%), Positives = 229/247 (91%)
Frame = +1
Query: 412 LLYMVLHARVVLDLCEQLEQEISFEQAEGDFDSFHGKWTFEQLGNHHTLLKYSVDSKMRR 471
LLYMVLHARVVLDLCE LEQEISFEQ EGDFDSF GKW FEQLGNHHTLLKYSV+SKMR+
Sbjct: 1 LLYMVLHARVVLDLCEYLEQEISFEQVEGDFDSFRGKWIFEQLGNHHTLLKYSVESKMRK 180
Query: 472 DTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEVCEQNTNSGQQIILSGSGDDNN 531
DTFLSEAIMEEVIYEDLPSNL AIRDY+EN AS++ EVCEQN NSGQQI+ SG D+N
Sbjct: 181 DTFLSEAIMEEVIYEDLPSNLSAIRDYIENMNASKYSEVCEQNMNSGQQILSSGYLKDDN 360
Query: 532 SSSADDISDCNVQSSSNQRSRVPGLQRDIEVLKSELLKFVAEYGQEGFMPMRKQLRLHGR 591
S SA+++ +C+VQ SS QRS+VPGLQR+I+VL+SELLKF+AE+GQEGFMPMRKQLRLHGR
Sbjct: 361 SGSAEEVPNCDVQCSSQQRSKVPGLQRNIKVLESELLKFIAEHGQEGFMPMRKQLRLHGR 540
Query: 592 VDIEKAITRMGGFRKIATIMNLSLAYKYRKPKGYWDNLENLQDEISRFQRCWGMDPSFMP 651
VDIEKAITRMGGFRKIATI+NLSLAYK+RKPKGYWDNLENLQ+EISRFQR WG+DPSFMP
Sbjct: 541 VDIEKAITRMGGFRKIATILNLSLAYKHRKPKGYWDNLENLQEEISRFQRGWGIDPSFMP 720
Query: 652 SRKSFER 658
SRKSFER
Sbjct: 721 SRKSFER 741
Score = 51.6 bits (122), Expect = 1e-06
Identities = 36/129 (27%), Positives = 60/129 (45%), Gaps = 1/129 (0%)
Frame = +1
Query: 164 MYWHIEARVVLDLQELLNSEWDRELHFSMVDGDFKKFEGKWSVKS-GTRSSSTNLSYEVN 222
+Y + ARVVLDL E L ++E+ F V+GDF F GKW + G + S E
Sbjct: 4 LYMVLHARVVLDLCEYL----EQEISFEQVEGDFDSFRGKWIFEQLGNHHTLLKYSVESK 171
Query: 223 VIPRFNFPAIFLERIVRSDLPVNLRALAYRVERNLLGNQKLPQPEDDLHKTSLVVNGSSV 282
+ +E ++ DLP NL A+ +E N+ ++ E +++ +++ +
Sbjct: 172 MRKDTFLSEAIMEEVIYEDLPSNLSAIRDYIE-NMNASKYSEVCEQNMNSGQQILSSGYL 348
Query: 283 KKINGSLCE 291
K N E
Sbjct: 349 KDDNSGSAE 375
Score = 40.0 bits (92), Expect(2) = e-124
Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +3
Query: 654 KSFERVVSQGKEKKNQTGQQPWQG*EK-*SQRISRCR**DQCSI*TLYFSRHTQVAYRTK 712
+ F V+QGK + QTGQ P G*E+ *S R **++ T+YFSRHT+VAY+TK
Sbjct: 792 RGFSASVAQGKTTE-QTGQ-PC*G*EEW*SYR-----**NED---TMYFSRHTKVAYKTK 941
Query: 713 RVGHKL 718
GH+L
Sbjct: 942 TFGHEL 959
>TC218194 similar to GB|AAO64894.1|29029030|BT005959 At5g08720 {Arabidopsis
thaliana;} , partial (24%)
Length = 659
Score = 320 bits (819), Expect = 2e-87
Identities = 157/194 (80%), Positives = 172/194 (87%)
Frame = +2
Query: 465 VDSKMRRDTFLSEAIMEEVIYEDLPSNLCAIRDYVENQKASQFLEVCEQNTNSGQQIILS 524
V+SKMR+DTFLSEAIMEEVIYEDLPSNLCAIRDY+EN+ AS E C+QNT+ GQQ + S
Sbjct: 2 VESKMRKDTFLSEAIMEEVIYEDLPSNLCAIRDYIENRTASNMFEACKQNTDLGQQTVPS 181
Query: 525 GSGDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVLKSELLKFVAEYGQEGFMPMRK 584
DD++ SA+D+ DCN QSSS QR RVPGLQRDIEVLKSELLKF+AE+GQEGFMPMRK
Sbjct: 182 VFEDDDSYCSAEDLFDCNAQSSSQQRPRVPGLQRDIEVLKSELLKFIAEHGQEGFMPMRK 361
Query: 585 QLRLHGRVDIEKAITRMGGFRKIATIMNLSLAYKYRKPKGYWDNLENLQDEISRFQRCWG 644
QLRLHGRVDIEKAI R GGFRKIATIMNLSLAYK+RKPKGYWDNLENL EISRFQR WG
Sbjct: 362 QLRLHGRVDIEKAINRKGGFRKIATIMNLSLAYKHRKPKGYWDNLENLHYEISRFQRSWG 541
Query: 645 MDPSFMPSRKSFER 658
MDPSFMPSR+SFER
Sbjct: 542 MDPSFMPSRRSFER 583
>CA938843
Length = 422
Score = 139 bits (350), Expect = 4e-33
Identities = 77/134 (57%), Positives = 92/134 (68%), Gaps = 10/134 (7%)
Frame = +2
Query: 30 ASSSRANPLSLR----SSLHSKPSFLSLSLFFPRHFHKS-IALSS---TTQCKPRSHLGG 81
++S+ P LR + HS FLSL L H S +ALSS TT KP H G
Sbjct: 32 STSTNVGPHPLRFLSKPTTHSSTRFLSLRL----HSRGSAVALSSSTRTTPLKPTCHFNG 199
Query: 82 NLNN--GLEEDGDREVHCELQVVSWRERRVKAEISINADINSVWNALTDYEHLADFIPNL 139
+ EE+G+REVHCE+QV+SWRERRV A+I+++AD SVWNALTDYEHLADFIPNL
Sbjct: 200 KDGSFEQHEEEGEREVHCEVQVISWRERRVNAQITVDADTESVWNALTDYEHLADFIPNL 379
Query: 140 VWSGRIPCPFPGRI 153
VWSGRIPCP+PGRI
Sbjct: 380 VWSGRIPCPYPGRI 421
Score = 36.2 bits (82), Expect = 0.052
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +2
Query: 357 RCVVASITVKAPVRDVWNVMSSYETLPEIVPNLAIS 392
R V A ITV A VWN ++ YE L + +PNL S
Sbjct: 281 RRVNAQITVDADTESVWNALTDYEHLADFIPNLVWS 388
>BQ273609
Length = 421
Score = 65.5 bits (158), Expect = 8e-11
Identities = 35/56 (62%), Positives = 39/56 (69%)
Frame = +3
Query: 156 EQRGFQRAMYWHIEARVVLDLQELLNSEWDRELHFSMVDGDFKKFEGKWSVKSGTR 211
+ RG + +Y AR VL L WDRELHFSMVDGDFKKF+GKWSVKSGTR
Sbjct: 252 KSRGEK*IIY*INTARFVL----LFLQAWDRELHFSMVDGDFKKFDGKWSVKSGTR 407
Score = 35.4 bits (80), Expect = 0.089
Identities = 16/43 (37%), Positives = 25/43 (57%)
Frame = +3
Query: 408 GCKGLLYMVLHARVVLDLCEQLEQEISFEQAEGDFDSFHGKWT 450
G K ++Y + AR VL + ++E+ F +GDF F GKW+
Sbjct: 261 GEK*IIY*INTARFVLLFLQAWDRELHFSMVDGDFKKFDGKWS 389
>TC230818 similar to UP|Q9LDH9 (Q9LDH9) Gb|AAF34826.1 (MJM20.1 protein),
partial (94%)
Length = 782
Score = 37.7 bits (86), Expect = 0.018
Identities = 32/120 (26%), Positives = 56/120 (46%), Gaps = 14/120 (11%)
Frame = -2
Query: 18 TLTLTSMITACRA-SSSRANPLSLRSSLH--SKP--SFLSLSLFFPRHFHKSIALSSTTQ 72
TL L +++ A + S S +NP SS H S P SF S+F +ST+
Sbjct: 520 TLKLLALLNASTSFSKSNSNPFFFNSSTHFPSNPLSSFSRCSIFS--------TWASTSL 365
Query: 73 CKPRSHLGGNLNNGL---------EEDGDREVHCELQVVSWRERRVKAEISINADINSVW 123
++H+ + NN +E+GD E+ ++VV W+ ++ + I+ + S+W
Sbjct: 364 L*RKAHIRPSENNREHSEGTHDL*QEEGDEELKRSMRVVEWKRKKKM*RVVISLRVESIW 185
>TC212585
Length = 1016
Score = 35.8 bits (81), Expect = 0.068
Identities = 18/56 (32%), Positives = 26/56 (46%)
Frame = -1
Query: 44 LHSKPSFLSLSLFFPRHFHKSIALSSTTQCKPRSHLGGNLNNGLEEDGDREVHCEL 99
++ KP FL + + F S+ LSS++ C RS N +NG E C L
Sbjct: 776 MNKKPKFLKILIKIEXAFVHSVMLSSSSSCSLRSRSASNSSNGTEXSSPSRKACTL 609
>TC208914
Length = 459
Score = 31.2 bits (69), Expect = 1.7
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +2
Query: 528 DDNNSSSADDISD-CNVQSSSNQRSRVPGLQRDIEVLKSELLKFVAEYGQEGFMPMRK 584
++ N++SAD +SD N SS++ GL + +K+ F G + F P+R+
Sbjct: 95 EEKNAASADSLSDEGNTNSSNSSGGSSSGLTSTWQSMKTGFQSFKVNIGAKKFFPLRQ 268
>TC226342 homologue to UP|NPM2_MOUSE (Q80W85) Nucleoplasmin 2, partial (8%)
Length = 1326
Score = 30.8 bits (68), Expect = 2.2
Identities = 27/104 (25%), Positives = 49/104 (46%), Gaps = 4/104 (3%)
Frame = +3
Query: 527 GDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDIE-VLKSELLK---FVAEYGQEGFMPM 582
G +SSS+ S + SS+ QR + + + IE +L LL F+ ++ Q F+ +
Sbjct: 66 GVRESSSSSSSSSSSSSSSSTYQRYDIQQVAKWIEQILPFSLLLLIVFIRQHLQGFFVTI 245
Query: 583 RKQLRLHGRVDIEKAITRMGGFRKIATIMNLSLAYKYRKPKGYW 626
+ +I K T + G RK++ ++ +S A+ YW
Sbjct: 246 CISAVMFKSNEIVKKQTALKGDRKVSVLLGISFAFVLHVISIYW 377
>TC227543
Length = 1489
Score = 30.4 bits (67), Expect = 2.9
Identities = 24/101 (23%), Positives = 40/101 (38%), Gaps = 17/101 (16%)
Frame = +2
Query: 268 DDLHKTSLVVNGSSVKKINGSLCETDKLAPGQD----------------KEGLDTSISGS 311
DD+H+ +V + + NGS + D + +D K G + +++
Sbjct: 581 DDMHRDGSLVKVNGISDTNGSGHQLDSVEETRDAINSRLQDSNHLVIKEKVGAEDALANG 760
Query: 312 LPASSSELNSNWGIFGKVCSLD-KPCVVDEVHLRRFDGLLE 351
P+S N+ I +LD KP D R G+LE
Sbjct: 761 FPSSGPSFNAGQVIEQNTKTLDNKPNTTDSSQERTAQGVLE 883
>TC219861 similar to UP|O24041 (O24041) Laccase , partial (14%)
Length = 534
Score = 29.6 bits (65), Expect = 4.9
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = +1
Query: 249 LAYRVERNLLGNQKLPQPEDDLHK 272
+A+ VE L NQKLP P DLHK
Sbjct: 175 MAWIVEDGKLPNQKLPPPPADLHK 246
>TC207159 similar to GB|AAR18374.1|38350523|AY444865 nucleobase-ascorbate
transporter 12 {Arabidopsis thaliana;} , partial (52%)
Length = 1445
Score = 29.6 bits (65), Expect = 4.9
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = -1
Query: 200 FEGKWSVKSGTRSSSTNLSYEVNVIPRF 227
F+G++ V S +R SS+NLS+ N+ P F
Sbjct: 1088 FQGQFIVISNSRLSSSNLSFGPNIYPTF 1005
>TC208150 similar to UP|Q6X182 (Q6X182) Mal d 1-associated protein, partial
(38%)
Length = 804
Score = 29.3 bits (64), Expect = 6.4
Identities = 30/112 (26%), Positives = 52/112 (45%), Gaps = 21/112 (18%)
Frame = +1
Query: 484 IYEDLPSNLCAIRDYVENQ------KASQFLEVCEQNTNSGQQIILSGSG------DDNN 531
+ D S CA V++Q + +F+ CE+ ++I+ + G N
Sbjct: 64 VRRDNSSERCATTKVVKSQCRTEEVETGKFVRKCEKT----EEILRNCIGKPAEVLQSNK 231
Query: 532 SSSADDISDCNVQ------SSSNQRSRV---PGLQRDIEVLKSELLKFVAEY 574
+ +DI+D ++ SSS+ S V PGLQ DIEV++ LL ++ +
Sbjct: 232 EYTEEDITDEVLKGRSVPFSSSDGASGVVDFPGLQNDIEVMERNLLSGLSHF 387
>TC224859 weakly similar to UP|Q7TP79 (Q7TP79) Aa2-245, partial (3%)
Length = 843
Score = 29.3 bits (64), Expect = 6.4
Identities = 25/82 (30%), Positives = 39/82 (47%)
Frame = -1
Query: 15 RLHTLTLTSMITACRASSSRANPLSLRSSLHSKPSFLSLSLFFPRHFHKSIALSSTTQCK 74
++ TLTL S ++ +SSS ++ +L SS S PS + FP S T Q K
Sbjct: 783 KMATLTLLSSLSLRSSSSSSSSSFTLSSSF-SSPSSFAFFCSFPLRSATSSLKVQTIQKK 607
Query: 75 PRSHLGGNLNNGLEEDGDREVH 96
R + +EED +E++
Sbjct: 606 KR------VVKAVEEDTQQELN 559
>TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family protein-like,
partial (29%)
Length = 971
Score = 28.9 bits (63), Expect = 8.3
Identities = 19/64 (29%), Positives = 31/64 (47%), Gaps = 2/64 (3%)
Frame = -3
Query: 3 TCHETSSTTTQL--RLHTLTLTSMITACRASSSRANPLSLRSSLHSKPSFLSLSLFFPRH 60
TC +S +++ R H L + S C +SSSR + S+ S PS L++ + P +
Sbjct: 744 TCLGLTSIPSRMKPRFHQLLVVSETLICNSSSSRPGLKNSPISISSHPSLLTVGRWSPWN 565
Query: 61 FHKS 64
S
Sbjct: 564 LSSS 553
>BI969600 similar to GP|10177224|dbj ribitol dehydrogenase-like {Arabidopsis
thaliana}, partial (13%)
Length = 655
Score = 28.9 bits (63), Expect = 8.3
Identities = 15/59 (25%), Positives = 24/59 (40%)
Frame = +1
Query: 505 SQFLEVCEQNTNSGQQIILSGSGDDNNSSSADDISDCNVQSSSNQRSRVPGLQRDIEVL 563
S ++ C Q ++ + LSG D + D ++ N S +N S L D L
Sbjct: 187 SNYVNACSQGASTTCYVALSGQTDGMSGKYFTDCNESNCSSLANDESEARKLWNDTHAL 363
>TC214632 homologue to UP|Q9LKH8 (Q9LKH8) NADPH-protochlorophyllide
oxidoreductase, complete
Length = 1525
Score = 28.9 bits (63), Expect = 8.3
Identities = 21/62 (33%), Positives = 28/62 (44%), Gaps = 3/62 (4%)
Frame = +1
Query: 5 HETSSTTTQLRLHTLTLTSMITACRASSSRANPLSLRSSLHSK---PSFLSLSLFFPRHF 61
+ T T +L H L T + + +A S P S SSLHSK P LS + R
Sbjct: 913 NSTEDTMMKLGSHLLPFTQVASPQQACSESTFPCSDFSSLHSKST*PRALSQKMNQERDL 1092
Query: 62 HK 63
H+
Sbjct: 1093HR 1098
>TC214136 similar to UP|Q84L12 (Q84L12) Peptide methionine sulfoxide
reductase, partial (71%)
Length = 1164
Score = 28.9 bits (63), Expect = 8.3
Identities = 24/71 (33%), Positives = 35/71 (48%)
Frame = +3
Query: 5 HETSSTTTQLRLHTLTLTSMITACRASSSRANPLSLRSSLHSKPSFLSLSLFFPRHFHKS 64
H S +T R + L S++ SSS ++PLSL S+ S FLS + + H+
Sbjct: 15 HRWSLSTKPQRSNCFLLLSLLPNPSLSSSSSSPLSLLSTTQSL-QFLSST----GNKHQH 179
Query: 65 IALSSTTQCKP 75
A SS+ C P
Sbjct: 180TATSSSLLCVP 212
>TC207258 similar to UP|IF2C_PHAVU (P57997) Translation initiation factor
IF-2, chloroplast precursor (PvIF2cp), partial (9%)
Length = 649
Score = 28.9 bits (63), Expect = 8.3
Identities = 17/58 (29%), Positives = 27/58 (46%)
Frame = -3
Query: 2 PTCHETSSTTTQLRLHTLTLTSMITACRASSSRANPLSLRSSLHSKPSFLSLSLFFPR 59
PT + + L +++ A R S S + S S HS+P+F SL++ F R
Sbjct: 266 PTPSTADAIEAEASSRVLFF*NVLKASRMSPSSQSSKSSNPSPHSRPAFTSLTISFTR 93
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,177,016
Number of Sequences: 63676
Number of extensions: 492544
Number of successful extensions: 2524
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 2485
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2518
length of query: 719
length of database: 12,639,632
effective HSP length: 104
effective length of query: 615
effective length of database: 6,017,328
effective search space: 3700656720
effective search space used: 3700656720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC148654.3