BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148653.2 + phase: 0
(117 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC224824 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, pa... 147 5e-37
TC225002 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, pa... 145 3e-36
TC207303 weakly similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like prot... 141 5e-35
BI788429 similar to GP|11596186|gb| cystatin-like protein {Citru... 132 2e-32
CD408043 weakly similar to PIR|T07822|T0782 cystein proteinase i... 118 3e-28
TC221610 90 1e-19
BE330223 90 2e-19
TC225963 homologue to UP|CYTI_VIGUN (Q06445) Cysteine proteinase... 75 3e-15
TC226074 cysteine proteinase inhibitor 70 1e-13
TC216246 UP|Q39842 (Q39842) Cysteine proteinase inhibitor (Fragm... 66 3e-12
TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inh... 65 4e-12
TC205866 UP|O04720 (O04720) Cysteine proteinase inhibitor, complete 65 5e-12
TC226073 similar to UP|Q71SU8 (Q71SU8) Protease inhibitor, parti... 62 3e-11
TC216658 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (68%) 57 1e-09
BU544869 53 2e-08
TC219907 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (64%) 53 2e-08
BE024068 similar to PIR|T07054|T070 cysteine proteinase inhibito... 49 3e-07
TC219423 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (66%) 45 4e-06
TC226075 similar to UP|Q39841 (Q39841) Cysteine proteinase inhib... 40 1e-04
TC229538 similar to UP|Q9SA76 (Q9SA76) T5I8.6, partial (29%) 30 0.16
>TC224824 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial (42%)
Length = 756
Score = 147 bits (372), Expect = 5e-37
Identities = 75/116 (64%), Positives = 90/116 (76%)
Frame = +3
Query: 1 MRNPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAK 60
MR+ LLLV L VL SYAA R++ A GGWSPI D+ND HV EIAN+A++EYD+RSGAK
Sbjct: 123 MRHQCLLLVSL---VLVSYAA-RSESALGGWSPIKDVNDSHVAEIANYALSEYDKRSGAK 290
Query: 61 LKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVPVH 116
L KV+ GE+QVV+GTNYRL L A DGS + +YEA+VWEK W HF NLTSF P+H
Sbjct: 291 LTLVKVVKGETQVVSGTNYRLVLKAKDGSATASYEAIVWEKPWLHFMNLTSFKPLH 458
>TC225002 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial (42%)
Length = 1205
Score = 145 bits (366), Expect = 3e-36
Identities = 70/108 (64%), Positives = 86/108 (78%)
Frame = +2
Query: 9 VLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVIN 68
+LL +VL SYAA R++ A GGWSPI D+ND HV EIAN+A++EYD+RSGAKL KV+
Sbjct: 290 LLLVSLVLVSYAA-RSESALGGWSPIKDVNDSHVAEIANYALSEYDKRSGAKLTLVKVVK 466
Query: 69 GESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVPVH 116
GE+QVV+GTNYRL L A DGS + +YEA+VWEK W HF NLTSF P+H
Sbjct: 467 GETQVVSGTNYRLVLKAKDGSATASYEAIVWEKPWLHFMNLTSFKPLH 610
>TC207303 weakly similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial
(47%)
Length = 479
Score = 141 bits (355), Expect = 5e-35
Identities = 71/112 (63%), Positives = 88/112 (78%), Gaps = 2/112 (1%)
Frame = +2
Query: 8 LVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVI 67
LV+L VVL + A ++ PGGW+PI +INDPHVTEIANFAVTEYD++SG KLK KVI
Sbjct: 59 LVVLVFVVLLACAVGWDEGIPGGWNPIKNINDPHVTEIANFAVTEYDKQSGEKLKLVKVI 238
Query: 68 NGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIW--QHFRNLTSFVPVHA 117
G+ QVVAG NYRL+L+ASD S NY+A+V+EK W +H+RNLTSF P+HA
Sbjct: 239 KGDLQVVAGLNYRLSLTASD---SNNYQAIVYEKAWAREHYRNLTSFTPLHA 385
>BI788429 similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (39%)
Length = 365
Score = 132 bits (332), Expect = 2e-32
Identities = 62/96 (64%), Positives = 75/96 (77%)
Frame = +1
Query: 21 AARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYR 80
AAR++ A GGWSPI D+ND HV EIAN+A++EYD+RSGAKL K GE+QV +GTNYR
Sbjct: 67 AARSESALGGWSPIKDVNDSHVAEIANYALSEYDKRSGAKLTLVKDGKGETQVGSGTNYR 246
Query: 81 LTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVPVH 116
L L A DGS + +YEA+VWEK W HF NLTSF P+H
Sbjct: 247 LVLKAKDGSATASYEAIVWEKPWLHFMNLTSFKPLH 354
>CD408043 weakly similar to PIR|T07822|T0782 cystein proteinase inhibitor -
cucumber, partial (53%)
Length = 606
Score = 118 bits (296), Expect = 3e-28
Identities = 59/88 (67%), Positives = 72/88 (81%), Gaps = 2/88 (2%)
Frame = -1
Query: 32 SPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYS 91
+PI +INDPHVTEIANFAVTEYD+ SG KLK KVI G+ QVV+G NYRL+L+ASD S
Sbjct: 474 NPIKNINDPHVTEIANFAVTEYDKLSGEKLKLVKVIKGDLQVVSGLNYRLSLTASD---S 304
Query: 92 KNYEAVVWEKIW--QHFRNLTSFVPVHA 117
NY+A+V+EK W +H+RNLTSF P+HA
Sbjct: 303 DNYQAIVYEKAWAREHYRNLTSFTPLHA 220
>TC221610
Length = 519
Score = 90.1 bits (222), Expect = 1e-19
Identities = 56/128 (43%), Positives = 74/128 (57%), Gaps = 12/128 (9%)
Frame = +3
Query: 2 RNPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKL 61
RN L LL V+VL + AR + G WSPI +INDP V EIAN+A+ +D+R G K
Sbjct: 36 RNMIKLECLLLVLVLMTSVVARKEPLLGRWSPIKNINDPKVIEIANYAIATFDKRFGTKK 215
Query: 62 KFEKVINGE----------SQVVAGTNYRLTLSASD--GSYSKNYEAVVWEKIWQHFRNL 109
K EK+I GE +++VAG +Y L A+D G + +NY A V HFRNL
Sbjct: 216 KLEKMIEGETRTVEGLKYKTRIVAGMDYFLIFRANDEYGEF-QNYGATVRVIESLHFRNL 392
Query: 110 TSFVPVHA 117
T FVP ++
Sbjct: 393 TGFVPANS 416
>BE330223
Length = 268
Score = 89.7 bits (221), Expect = 2e-19
Identities = 48/90 (53%), Positives = 60/90 (66%)
Frame = +2
Query: 10 LLFVVVLFSYAAARNQFAPGGWSPIDDINDPHVTEIANFAVTEYDRRSGAKLKFEKVING 69
LL +VL SYAA R + GG SPI D+ND HV EI N+A++EY+ R+GAK K I G
Sbjct: 2 LLVSLVLVSYAA-RAEITLGGLSPIKDVNDSHVAEIGNYALSEYEERAGAKRTLGKGIKG 178
Query: 70 ESQVVAGTNYRLTLSASDGSYSKNYEAVVW 99
E+Q V+ TN L L A DGS + YEA+VW
Sbjct: 179ENQGVSRTNCPLVLKAKDGSGTARYEAIVW 268
>TC225963 homologue to UP|CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor
(Cystatin), complete
Length = 757
Score = 75.5 bits (184), Expect = 3e-15
Identities = 34/75 (45%), Positives = 49/75 (65%)
Frame = +3
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E++++ A L+FEKV++ + QVV+GT Y +TL A DG K YEA
Sbjct: 198 NSLEIDGLARFAVEEHNKKQNALLEFEKVVSAKQQVVSGTLYTITLEAKDGGQKKVYEAK 377
Query: 98 VWEKIWQHFRNLTSF 112
VWEK W +F+ + F
Sbjct: 378 VWEKAWLNFKEVQEF 422
>TC226074 cysteine proteinase inhibitor
Length = 828
Score = 70.5 bits (171), Expect = 1e-13
Identities = 33/79 (41%), Positives = 47/79 (58%)
Frame = +2
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E ++R + L+F +VI+ + QVVAG NY +TL A DG YEA
Sbjct: 53 NSVEINNLARFAVEEQNKRENSVLEFVRVISAKQQVVAGVNYYITLEAKDGLIKNEYEAK 232
Query: 98 VWEKIWQHFRNLTSFVPVH 116
VW + W + + L F PV+
Sbjct: 233 VWVREWLNSKELLEFKPVN 289
Score = 57.4 bits (137), Expect = 1e-09
Identities = 27/78 (34%), Positives = 42/78 (53%)
Frame = +2
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV +++++ A L+F VI + QVV G Y +TL A DG YE
Sbjct: 332 NSLEIDILARFAVDQHNKKENANLEFVGVIRAKQQVVEGFIYYITLEAKDGETKNVYETK 511
Query: 98 VWEKIWQHFRNLTSFVPV 115
VW + W + + + F P+
Sbjct: 512 VWVRSWLNSKEVLEFKPI 565
>TC216246 UP|Q39842 (Q39842) Cysteine proteinase inhibitor (Fragment),
complete
Length = 715
Score = 65.9 bits (159), Expect = 3e-12
Identities = 35/90 (38%), Positives = 54/90 (59%), Gaps = 1/90 (1%)
Frame = +2
Query: 27 APGGWSPIDDI-NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSA 85
A GG++ I N + +A FAV E++++ A L+F +VI+ + QVV+GT Y +TL A
Sbjct: 50 ALGGFTDITGAQNSIDIENLARFAVDEHNKKENAVLEFVRVISAKKQVVSGTLYYITLEA 229
Query: 86 SDGSYSKNYEAVVWEKIWQHFRNLTSFVPV 115
+DG K YE V EK W + + + F P+
Sbjct: 230 NDGVTKKVYETKVLEKPWLNIKEVQEFKPI 319
>TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inhibitor,
complete
Length = 1064
Score = 65.5 bits (158), Expect = 4e-12
Identities = 31/77 (40%), Positives = 45/77 (58%)
Frame = +1
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N +A FAV E++++ + L+F +V+ + QVVAGT + LTL A + K YEA
Sbjct: 220 NSVETEALARFAVDEHNKKQNSLLEFARVVRAQEQVVAGTLHHLTLEAIEAGEKKLYEAK 399
Query: 98 VWEKIWQHFRNLTSFVP 114
VW K W +F+ L F P
Sbjct: 400 VWVKPWLNFKELQEFKP 450
>TC205866 UP|O04720 (O04720) Cysteine proteinase inhibitor, complete
Length = 1175
Score = 65.1 bits (157), Expect = 5e-12
Identities = 31/77 (40%), Positives = 45/77 (58%)
Frame = +3
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N +A FAV E++++ + L+F +V+ + QVVAGT + LTL A + K YEA
Sbjct: 222 NSVQTEALARFAVDEHNKKQNSLLEFSRVVRTQEQVVAGTLHHLTLEAIEAGEKKLYEAK 401
Query: 98 VWEKIWQHFRNLTSFVP 114
VW K W +F+ L F P
Sbjct: 402 VWVKPWLNFKELQEFKP 452
>TC226073 similar to UP|Q71SU8 (Q71SU8) Protease inhibitor, partial (75%)
Length = 666
Score = 62.4 bits (150), Expect = 3e-11
Identities = 30/77 (38%), Positives = 45/77 (57%)
Frame = +3
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + +A FAV E ++R + L+F +VI+ + QVV+G NY +TL A DG Y+A
Sbjct: 75 NSVEINNLARFAVEEQNKRENSVLEFVRVISAQQQVVSGVNYYITLEAKDGEIKNEYKAK 254
Query: 98 VWEKIWQHFRNLTSFVP 114
VWE+ + L F+P
Sbjct: 255 VWER---ESQELLEFMP 296
Score = 58.5 bits (140), Expect = 4e-10
Identities = 28/74 (37%), Positives = 40/74 (53%)
Frame = +3
Query: 42 VTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEK 101
+ +A FAV E ++R A L+F VI + QVV G Y +TL A DG YE VW +
Sbjct: 318 IDHLARFAVEEQNKRENANLEFVGVIRAKQQVVEGFIYYITLEAKDGETKNVYETKVWVR 497
Query: 102 IWQHFRNLTSFVPV 115
W + + + F P+
Sbjct: 498 SWLNSKEVLEFKPI 539
>TC216658 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (68%)
Length = 849
Score = 57.4 bits (137), Expect = 1e-09
Identities = 39/117 (33%), Positives = 58/117 (49%), Gaps = 8/117 (6%)
Frame = +2
Query: 6 LLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN-DPHVTEIANFAVTEYDR-------RS 57
L +VL + VL S + AR GG + + D+ + V E+ FAV EY+R
Sbjct: 218 LTIVLSLLSVLSSASCAR---MVGGKTEVPDVRTNREVQELGRFAVEEYNRGLKQWKNNG 388
Query: 58 GAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVP 114
+L F +V+ + QVV+G Y L +SA+ K + +VV K W H + L F P
Sbjct: 389 SEQLNFSEVVEAQQQVVSGMKYYLKISATHKGVHKMFTSVVVVKPWLHSKQLLHFAP 559
>BU544869
Length = 588
Score = 52.8 bits (125), Expect = 2e-08
Identities = 25/54 (46%), Positives = 33/54 (60%)
Frame = -2
Query: 61 LKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVP 114
L+F +V+ + QVVAGT + LTL A + K YEA VW K W +F+ L F P
Sbjct: 275 LEFSRVVRTQEQVVAGTLHHLTLEAIEAGEKKLYEAKVWVKPWLNFKELQEFKP 114
>TC219907 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (64%)
Length = 553
Score = 52.8 bits (125), Expect = 2e-08
Identities = 35/124 (28%), Positives = 65/124 (52%), Gaps = 10/124 (8%)
Frame = +3
Query: 1 MRNPTLLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN-DPHVTEIANFAVTEYDR---- 55
+R+P ++L +L + + A+ GG S I D+ + V ++ F+V E++R
Sbjct: 48 IRSPAVILAILTISACIACTASYGGLV-GGRSKIPDVKANKKVQDLGRFSVEEHNRMLRQ 224
Query: 56 --RSGAKLKFEKVINGESQVVAGTNYRLTLSAS---DGSYSKNYEAVVWEKIWQHFRNLT 110
+ ++ F +V+ + QVV+G Y + +SA+ DG S+ +E+VV K W + L
Sbjct: 225 AQKEEEQVTFVEVVEAQQQVVSGIKYYMKISATQGGDGGDSRIFESVVVVKPWLRSKQLL 404
Query: 111 SFVP 114
+F P
Sbjct: 405 NFAP 416
>BE024068 similar to PIR|T07054|T070 cysteine proteinase inhibitor (clone R1)
- soybean (fragment), partial (88%)
Length = 265
Score = 49.3 bits (116), Expect = 3e-07
Identities = 24/70 (34%), Positives = 42/70 (59%)
Frame = +2
Query: 38 NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97
N + ++ FAV E++ + A+L+F + ++G+ QVVAGT + A+DG K Y+
Sbjct: 29 NSIDIEKLGRFAVDEHNIKENARLEFVRGMSGKKQVVAGTLNCMAGEANDGVTKKGYDTN 208
Query: 98 VWEKIWQHFR 107
V +K W++ R
Sbjct: 209 VLDKPWKNIR 238
>TC219423 similar to UP|Q84XY6 (Q84XY6) Cystatin, partial (66%)
Length = 440
Score = 45.4 bits (106), Expect = 4e-06
Identities = 34/119 (28%), Positives = 55/119 (45%), Gaps = 12/119 (10%)
Frame = +3
Query: 6 LLLVLLFVVVLFSYAAARNQFAPGGWSPIDDIN-DPHVTEIANFAVTEYDR--------- 55
L +++ + VL S + AR GG + I ++ + V E+ FAV EY+
Sbjct: 93 LTILVTLLSVLSSASCAR---MVGGKTEIPEVRKNRQVQELGRFAVEEYNLGLKLLKNNN 263
Query: 56 --RSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSF 112
+L F V+ + QVV+G Y L +SA+ + + +VV K W H + L F
Sbjct: 264 VDNGREQLNFSAVVEAQQQVVSGMKYYLKISATHNGVHEMFNSVVVVKPWLHSKQLLHF 440
>TC226075 similar to UP|Q39841 (Q39841) Cysteine proteinase inhibitor
(Fragment), partial (33%)
Length = 427
Score = 40.4 bits (93), Expect = 1e-04
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +1
Query: 70 ESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHFRNLTSFVP 114
+ QVV+G NY +TL A DG Y+A VWE+ + L F+P
Sbjct: 13 QQQVVSGVNYYITLEAKDGEIKNEYKAKVWER---ESQELLEFMP 138
>TC229538 similar to UP|Q9SA76 (Q9SA76) T5I8.6, partial (29%)
Length = 1321
Score = 30.0 bits (66), Expect = 0.16
Identities = 15/58 (25%), Positives = 30/58 (50%)
Frame = +2
Query: 49 AVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAVVWEKIWQHF 106
A E+ AK+ FE++ +++ T+Y++ + S++ +A V EK W +F
Sbjct: 641 AYLEHGMFQEAKVLFEQMSENANRLRGNTDYKMLVVPDTYSFNTMLDACVAEKRWDYF 814
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,221,039
Number of Sequences: 63676
Number of extensions: 55939
Number of successful extensions: 350
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 342
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 344
length of query: 117
length of database: 12,639,632
effective HSP length: 93
effective length of query: 24
effective length of database: 6,717,764
effective search space: 161226336
effective search space used: 161226336
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148653.2