BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.7 - phase: 0 /pseudo
(1391 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU081086 weakly similar to GP|27817864|db OJ1081_B12.13 {Oryza s... 199 9e-51
CD402931 similar to GP|9081793|dbj P0489A01.23 {Oryza sativa (ja... 149 3e-41
TC213121 122 1e-27
TC231883 weakly similar to UP|Q7QBQ3 (Q7QBQ3) AgCP4056 (Fragment... 107 4e-23
TC212860 weakly similar to UP|PIF1_YEAST (P07271) DNA repair and... 40 0.005
TC224067 36 0.10
TC228663 similar to UP|Q9SE92 (Q9SE92) Adenosine-5'-phosphosulfa... 32 1.5
TC215239 UP|H3_PEA (P02300) Histone H3, partial (39%) 32 1.9
TC210380 similar to PIR|B96789|B96789 protein T23E18.4 [imported... 32 1.9
TC234535 similar to UP|Q9S960 (Q9S960) LEAUX=ARABIDOPSIS auxin-r... 32 1.9
TC209328 similar to UP|KAPS_CATRO (O49204) Adenylyl-sulfate kina... 31 3.3
TC206099 similar to UP|O82161 (O82161) Phi-1 protein, partial (85%) 30 5.7
TC205614 30 7.4
AW100554 weakly similar to SP|P10976|NLTP_ Nonspecific lipid-tra... 30 9.7
>BU081086 weakly similar to GP|27817864|db OJ1081_B12.13 {Oryza sativa
(japonica cultivar-group)}, partial (7%)
Length = 426
Score = 199 bits (505), Expect = 9e-51
Identities = 100/142 (70%), Positives = 116/142 (81%)
Frame = +2
Query: 1207 SDSIDRSEVNDQCQSFQLFTPEFLSTLRTSGLPNHKIKLKVGTPIMLLRNLDQVEGLCNG 1266
SDSI++S+ + + F T EF+++L TS LPNHKIKLKV + IMLLRNLDQ EGLCN
Sbjct: 2 SDSIEKSDTIES-EGFSTITTEFINSLSTSRLPNHKIKLKVDSRIMLLRNLDQNEGLCNS 178
Query: 1267 TRLIVTRMANHVIEAKIISGKNVGNLTYIPRMSMSPSQSPWPFKLIRRQFPIIVSYAMTI 1326
TRLI+T A+H+IEAKI+SGK GN YIPR++ SPSQSPWPFKLIRR+FPIIVSYAMTI
Sbjct: 179 TRLIITMFADHIIEAKIMSGKGQGNTVYIPRLATSPSQSPWPFKLIRRKFPIIVSYAMTI 358
Query: 1327 NKSQGQTLDSVGLYLPRSVFSH 1348
NKSQGQ L SVGLYLP VFSH
Sbjct: 359 NKSQGQLLASVGLYLPTPVFSH 424
>CD402931 similar to GP|9081793|dbj P0489A01.23 {Oryza sativa (japonica
cultivar-group)}, partial (5%)
Length = 617
Score = 149 bits (377), Expect(2) = 3e-41
Identities = 81/145 (55%), Positives = 105/145 (71%), Gaps = 2/145 (1%)
Frame = +2
Query: 941 GTGKTFMWTTLAASLRSQGKIVLTVATSGIASLLLPGGRTAHSKFKIPVPSFENSTCNID 1000
GT KTF+W TL+A +RS+G ++ + +ASLLLP G TAHS F IP+ ++STCNI+
Sbjct: 11 GTSKTFLWKTLSAGMRSKG-LMSSSCLKRLASLLLPRG*TAHSTFSIPLVIKDDSTCNIN 187
Query: 1001 GDSDLAKLLKVTNLIIWDEAPMAHKFCFEALDRTLKDIMS--DTADGDKIFGGKVIVFGG 1058
S +KLL T LIIWDE P+ +KFCFEALDRTL+DIM+ + + K FGGKV V GG
Sbjct: 188 QGSARSKLLLHTKLIIWDETPVMNKFCFEALDRTLQDIMASQNKDNATKPFGGKV-VLGG 364
Query: 1059 DFRQILPVVPRADRSDIINSSINSS 1083
DFRQILPV+ + R DI+ S+IN+S
Sbjct: 365 DFRQILPVIRKGSRQDIVGSAINAS 439
Score = 39.3 bits (90), Expect(2) = 3e-41
Identities = 19/49 (38%), Positives = 30/49 (60%)
Frame = +1
Query: 1096 KNMRLRFNVGSSDADELKTFSEWILKVGEGKISEPNDGIVDFEIPDDLL 1144
KNMRL + D D+++ F +WIL + +G E ++G +D IP +LL
Sbjct: 460 KNMRLGTTNNNEDRDDIREFVDWILMIRDGNRDEEDEGEID--IPKNLL 600
>TC213121
Length = 713
Score = 122 bits (305), Expect = 1e-27
Identities = 57/112 (50%), Positives = 80/112 (70%)
Frame = +2
Query: 866 SLKEFKGFPYPTDYVMEELGNRLIYDELNYDEDALKLEFKRLFSTVTDEQRDIYHKIMSA 925
S + F YP Y++ GN+LIY+EL YD+D L F R + ++TDE I++KIM
Sbjct: 275 SHRNFPLMSYPIGYIVNPYGNKLIYNELAYDKDILAA*FSRCYHSLTDEHASIFNKIMHM 454
Query: 926 VNGQNGGVFFLHGYGGTGKTFMWTTLAASLRSQGKIVLTVATSGIASLLLPG 977
+ Q+GGV+FL+GYGGTGKTF+W TL++++ S IVLT+A+SGI S+LLPG
Sbjct: 455 IATQSGGVYFLYGYGGTGKTFVWKTLSSTIHSNSGIVLTMASSGILSMLLPG 610
>TC231883 weakly similar to UP|Q7QBQ3 (Q7QBQ3) AgCP4056 (Fragment), partial
(17%)
Length = 1263
Score = 107 bits (267), Expect = 4e-23
Identities = 47/74 (63%), Positives = 59/74 (79%)
Frame = +1
Query: 435 MYTIEFQKRGLPHAHILIFLHPSSKYPTPDHINQIISAEIPHPENDRELYKLVGTHMMHG 494
M+TIEFQKRGLPH +L+FLHP ++YP+ D I+ IISAEIP E+D ELY LV HM+HG
Sbjct: 877 MHTIEFQKRGLPHIQLLLFLHPDNQYPSSDEIDHIISAEIPSHEDDPELYTLVQNHMVHG 1056
Query: 495 PCGLAKPSSPCMKK 508
PCG+ + SPCMK+
Sbjct: 1057PCGILQSHSPCMKE 1098
>TC212860 weakly similar to UP|PIF1_YEAST (P07271) DNA repair and recombination
protein PIF1, mitochondrial precursor, partial (8%)
Length = 596
Score = 40.4 bits (93), Expect = 0.005
Identities = 24/67 (35%), Positives = 38/67 (55%)
Frame = +2
Query: 1321 SYAMTINKSQGQTLDSVGLYLPRSVFSHGQLYVAFSRVRTKAGLKILIHDLEKKPLSNTT 1380
++AM+I+K QG TL+ V L R+ F G +YVA SRVR+ GL + + K +
Sbjct: 2 AWAMSIHKCQGMTLERVHTDLSRA-FGCGMVYVALSRVRSLEGLHLSAFNRSKIKVDQRV 178
Query: 1381 NVVYKEV 1387
+ Y+ +
Sbjct: 179 SRFYRSL 199
>TC224067
Length = 415
Score = 36.2 bits (82), Expect = 0.10
Identities = 29/71 (40%), Positives = 39/71 (54%), Gaps = 7/71 (9%)
Frame = -2
Query: 1319 IVSYAMTINKSQGQTLDSV-GLYLPR-SVFSHGQLYVAFSRV-----RTKAGLKILIHDL 1371
+V Y M INKS GQTL V G+ LPR + SHGQ YV ++V R + +DL
Sbjct: 363 LVCYTMKINKSHGQTLSQVGGVLLPRPN**SHGQ-YVTLNQV*SY*RRKSKSINCEKYDL 187
Query: 1372 EKKPLSNTTNV 1382
++ N T+V
Sbjct: 186 QRIFSKNITHV 154
>TC228663 similar to UP|Q9SE92 (Q9SE92) Adenosine-5'-phosphosulfate kinase
(Fragment) , partial (68%)
Length = 940
Score = 32.3 bits (72), Expect = 1.5
Identities = 26/87 (29%), Positives = 39/87 (43%)
Frame = +3
Query: 929 QNGGVFFLHGYGGTGKTFMWTTLAASLRSQGKIVLTVATSGIASLLLPGGRTAHSKFKIP 988
Q G V ++ G G+GK+ + +L+ L S+GK+ S +L G H K
Sbjct: 201 QKGCVVWITGLSGSGKSTLACSLSRELHSRGKL----------SYVLDGDNLRHGLNKDL 350
Query: 989 VPSFENSTCNIDGDSDLAKLLKVTNLI 1015
E+ T NI ++AKL LI
Sbjct: 351 GFKAEDRTENIRRTGEVAKLFADAGLI 431
>TC215239 UP|H3_PEA (P02300) Histone H3, partial (39%)
Length = 340
Score = 32.0 bits (71), Expect = 1.9
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = -3
Query: 1316 FPIIVSYAMTINKSQGQT 1333
FP+IV +AMT NKS+GQT
Sbjct: 326 FPLIVCFAMTTNKSEGQT 273
>TC210380 similar to PIR|B96789|B96789 protein T23E18.4 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(32%)
Length = 807
Score = 32.0 bits (71), Expect = 1.9
Identities = 23/73 (31%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = +3
Query: 277 SRKTEAMTILCARRLFQQFLVDCFTMMEADRLKW-LRKNQSKLRVGKYRSLSNNQGNVDG 335
SR+ + I C L ++FL CFT++ W L +RV YRS +N V G
Sbjct: 111 SRENLTVVI*CP*SLVRRFLEGCFTILS----NWFLHHRFQNMRVPLYRSTANLTVLVVG 278
Query: 336 QTKKQGKRVVLPS 348
+ K+G ++ S
Sbjct: 279 RKTKEGGTPIIQS 317
>TC234535 similar to UP|Q9S960 (Q9S960) LEAUX=ARABIDOPSIS auxin-regulated
protein homolog (Fragment), partial (11%)
Length = 530
Score = 32.0 bits (71), Expect = 1.9
Identities = 18/66 (27%), Positives = 29/66 (43%)
Frame = +3
Query: 15 NPEFGMCCGNGKIQIPFLPTPPPLLNDLMFNNYSTESKQFNQNIRLYNSMFAFSSPGFKV 74
NP FG+ CG I L +++ + + NQ +R+ N+ F+ S P
Sbjct: 153 NPGFGISCGPNGFPILNLSNTQYIIHQIFYE---------NQTLRVSNTAFSVSRPNTTN 305
Query: 75 DKGVIP 80
KG +P
Sbjct: 306 SKGCLP 323
>TC209328 similar to UP|KAPS_CATRO (O49204) Adenylyl-sulfate kinase,
chloroplast precursor (APS kinase)
(Adenosine-5'phosphosulfate kinase) (ATP
adenosine-5'-phosphosulfate 3'-phosphotransferase) ,
partial (69%)
Length = 1056
Score = 31.2 bits (69), Expect = 3.3
Identities = 26/87 (29%), Positives = 39/87 (43%)
Frame = +1
Query: 929 QNGGVFFLHGYGGTGKTFMWTTLAASLRSQGKIVLTVATSGIASLLLPGGRTAHSKFKIP 988
Q G V +L G G+GKT + L+ SL S+GK+ S +L G H +
Sbjct: 244 QKGCVIWLTGLSGSGKTTIACALSRSLHSKGKL----------SYILDGDNIRHGLNQDL 393
Query: 989 VPSFENSTCNIDGDSDLAKLLKVTNLI 1015
E+ + NI ++AKL +I
Sbjct: 394 SFRAEDRSENIRRIGEVAKLFADAGVI 474
>TC206099 similar to UP|O82161 (O82161) Phi-1 protein, partial (85%)
Length = 1174
Score = 30.4 bits (67), Expect = 5.7
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = +2
Query: 440 FQKRGLPHAHILIFLHPSSKYPTPDHINQIIS 471
FQ LP +I +FLHPSS PT H+++II+
Sbjct: 2 FQFPSLP-IYIHLFLHPSSPQPTCFHLHKIIN 94
>TC205614
Length = 742
Score = 30.0 bits (66), Expect = 7.4
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1
Query: 622 LSLCFT*RSLLEDICLSNPWKKASC*KIVLS 652
LS+ FT S+LE+ LSN WK + *+++LS
Sbjct: 631 LSIHFTM*SMLENFLLSNLWKNSYS*EVILS 723
>AW100554 weakly similar to SP|P10976|NLTP_ Nonspecific lipid-transfer
protein precursor (LTP) (Phospholipid transfer protein)
(PLTP). [Spinach], partial (21%)
Length = 307
Score = 29.6 bits (65), Expect = 9.7
Identities = 19/72 (26%), Positives = 29/72 (39%), Gaps = 9/72 (12%)
Frame = +1
Query: 25 GKIQIPFLPTPPPLLNDLMFNNYSTESKQFNQNIRLY---------NSMFAFSSPGFKVD 75
G ++ P P P ND+ N T++ ++Q I +Y NS+ P
Sbjct: 88 GYLKGPGGSVPTPCYNDINIINNQTKTTPYHQEIYIYLKNTILILLNSILQPFHPSLSSI 267
Query: 76 KGVIPGRGPPTI 87
K P R PP +
Sbjct: 268 KSTYPTRSPPPL 303
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.336 0.147 0.471
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,777,174
Number of Sequences: 63676
Number of extensions: 864128
Number of successful extensions: 6189
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 6107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6187
length of query: 1391
length of database: 12,639,632
effective HSP length: 109
effective length of query: 1282
effective length of database: 5,698,948
effective search space: 7306051336
effective search space used: 7306051336
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 65 (29.6 bits)
Medicago: description of AC148528.7