BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148528.6 + phase: 0 /partial
(310 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208526 similar to UP|Q6YW97 (Q6YW97) MAP3K protein kinase-like... 244 3e-65
CO985741 137 5e-33
BU577276 40 0.001
CO983198 30 1.1
TC230000 UP|Q43439 (Q43439) Phosphatidylinositol-specific phosph... 30 1.4
TC203547 weakly similar to UP|Q75IX4 (Q75IX4) Expressed protein,... 30 1.8
AW164281 similar to PIR|T11688|T11 phosphoinositide-specific pho... 29 3.1
BE058237 28 4.1
TC233681 UP|Q43442 (Q43442) Phosphoinositide-specific phospholip... 28 5.3
TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specif... 28 6.9
TC216905 28 6.9
TC217319 similar to UP|Q8SNA3 (Q8SNA3) Bat3 protein (Fragment), ... 27 9.0
>TC208526 similar to UP|Q6YW97 (Q6YW97) MAP3K protein kinase-like protein,
partial (59%)
Length = 1214
Score = 244 bits (624), Expect = 3e-65
Identities = 115/217 (52%), Positives = 154/217 (69%), Gaps = 3/217 (1%)
Frame = +2
Query: 93 LDMHDYYGDIWLC---RGPCTIFTAFQPAINVLREINTFLTRHRTEIVTVFIKDRVTSPN 149
LDM+D+ DIWLC RG C FTAFQPA+N L+E+ FLT + TEIVT+ I+D V +P
Sbjct: 23 LDMYDFQNDIWLCHSFRGQCYNFTAFQPAVNTLKEVEAFLTENPTEIVTIIIEDYVHTPK 202
Query: 150 GVNKVFNKAGLRKFWFPVYKMPKNGSDWLTVKKMLRMNHRLIVFTSNATKEASERIAYEW 209
G+ VF AGL K+WFPV KMPK G DW TV +M++ NHRL+VFTS+A+KEA E IAY+W
Sbjct: 203 GLTNVFTSAGLDKYWFPVSKMPKKGDDWPTVTEMVQANHRLVVFTSDASKEAGEGIAYQW 382
Query: 210 NYVVENKYGNDGMGRDHCLHRAESYPMNTTTKSLVLMNYYRNVLNSNEACKDNSSPLIRK 269
++VEN+ G+ G+ + C HR ES +N+ + SL LMNY+ ++CK++S+PL
Sbjct: 383 KHMVENESGDPGVQQGSCPHRKESKALNSKSHSLFLMNYFPTYPVEADSCKEHSAPLAEM 562
Query: 270 MHTCYKDAGNRWPNYIAVDFYKRGDGGGAPEALDVAN 306
++TCYK AGN PN+IAV+FY R DGGG + +D N
Sbjct: 563 VNTCYKAAGNLMPNFIAVNFYMRSDGGGVFDIVDKMN 673
>CO985741
Length = 761
Score = 137 bits (346), Expect = 5e-33
Identities = 63/107 (58%), Positives = 82/107 (75%)
Frame = -1
Query: 203 ERIAYEWNYVVENKYGNDGMGRDHCLHRAESYPMNTTTKSLVLMNYYRNVLNSNEACKDN 262
E IAY+W YVVEN+YG+DGM C RAES MNT ++SLVL+NY+ + N ++AC DN
Sbjct: 761 EGIAYQWTYVVENQYGDDGMKAGSCPSRAESPAMNTKSRSLVLVNYFHSAPNRSQACADN 582
Query: 263 SSPLIRKMHTCYKDAGNRWPNYIAVDFYKRGDGGGAPEALDVANRNL 309
S+PL+ M TC++ AGNRW N+IAVD+Y+R DGGGAP A+D AN +L
Sbjct: 581 SAPLLDMMKTCHEAAGNRWANFIAVDYYQRSDGGGAPLAVDEANGHL 441
>BU577276
Length = 428
Score = 40.0 bits (92), Expect = 0.001
Identities = 25/53 (47%), Positives = 28/53 (52%), Gaps = 4/53 (7%)
Frame = +2
Query: 1 QIGETCSRDVNDCGTGLQCLECNS----QSRCTRVRTSSPISKVMELPFNHYS 49
QI E CS DCG GL C C S Q CTR + + P S V LPFN Y+
Sbjct: 272 QILEACSA-ATDCGPGLFCGNCPSLGLKQPICTRGQVTLPTSIVNGLPFNKYT 427
>CO983198
Length = 736
Score = 30.4 bits (67), Expect = 1.1
Identities = 14/37 (37%), Positives = 22/37 (58%)
Frame = -1
Query: 257 EACKDNSSPLIRKMHTCYKDAGNRWPNYIAVDFYKRG 293
++CK++ PL ++TCYK AGN +AV + G
Sbjct: 166 DSCKEHLVPLADMVNTCYKAAGNHVLPNLAVKRHVNG 56
>TC230000 UP|Q43439 (Q43439) Phosphatidylinositol-specific phospholipase C ,
complete
Length = 2116
Score = 30.0 bits (66), Expect = 1.4
Identities = 21/55 (38%), Positives = 27/55 (48%)
Frame = +1
Query: 41 MELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDM 95
M LP +HY T HNSY + LS D ++N L+ GVR I LD+
Sbjct: 439 MTLPLSHYFIYTGHNSYLT-GNQLSSDCSDVPIIN-------ALKRGVRVIELDI 579
>TC203547 weakly similar to UP|Q75IX4 (Q75IX4) Expressed protein, partial
(6%)
Length = 302
Score = 29.6 bits (65), Expect = 1.8
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +3
Query: 257 EACKDNSSPLIRKMHTCYKDAGN 279
++CK++ PL ++TCYK AGN
Sbjct: 39 DSCKEHLVPLADMVNTCYKAAGN 107
>AW164281 similar to PIR|T11688|T11 phosphoinositide-specific phospholipase C
(EC 3.1.4.-) - cowpea, partial (21%)
Length = 433
Score = 28.9 bits (63), Expect = 3.1
Identities = 36/117 (30%), Positives = 50/117 (41%), Gaps = 11/117 (9%)
Frame = +2
Query: 41 MELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDM--HDY 98
M P +HY T HNSY + + S S V I L+ GVR I LD+ +
Sbjct: 68 MTAPLSHYFIYTGHNSYLT---GNQLSSDCSDV-----PIIKALQRGVRVIELDLWPNSD 223
Query: 99 YGDIWLCRGPC-----TIFTAFQPA--INVLREIN--TFLTRHRTEIVTVFIKDRVT 146
DI + G C +I T P I L+ I F+ H I+T ++D +T
Sbjct: 224 EDDIEVVHGRC*LILSSISTLTTPVSLIQCLKSIKEYAFVKSHYPLIIT--LEDHLT 388
>BE058237
Length = 413
Score = 28.5 bits (62), Expect = 4.1
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -2
Query: 261 DNSSPLIRKMHTCYKDAGNRWPNYIAVDFYKRG 293
+NS LI + TC+ NY+AVD+YK G
Sbjct: 376 NNSGGLIDMLQTCHG------ANYVAVDYYKAG 296
>TC233681 UP|Q43442 (Q43442) Phosphoinositide-specific phospholipase C P12 ,
complete
Length = 1880
Score = 28.1 bits (61), Expect = 5.3
Identities = 32/114 (28%), Positives = 46/114 (40%), Gaps = 2/114 (1%)
Frame = +1
Query: 35 SPISKVMELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLD 94
S + M P +HY T HNSY + + S S V I L+ GVR I LD
Sbjct: 298 SQVHHDMNAPLSHYFIYTGHNSYLT---GNQLSSDCSDV-----PIIKALQRGVRVIELD 453
Query: 95 M--HDYYGDIWLCRGPCTIFTAFQPAINVLREINTFLTRHRTEIVTVFIKDRVT 146
+ + DI + G TA I L+ I + V + ++D +T
Sbjct: 454 LWPNSTKDDIDVVHG--RTLTAPVSLIQCLKSIKEYAFVKSDYPVIITLEDHLT 609
>TC218867 homologue to UP|Q43444 (Q43444) Phosphoinositide-specific
phospholipase C P25 , complete
Length = 2341
Score = 27.7 bits (60), Expect = 6.9
Identities = 20/55 (36%), Positives = 26/55 (46%)
Frame = +3
Query: 41 MELPFNHYSWLTTHNSYASRAANLSIDSKISSVMNQEDSITDQLRNGVRGIMLDM 95
M P +HY T HNSY + LS D ++N L+ GVR I LD+
Sbjct: 342 MSSPLSHYFIYTGHNSYLT-GNQLSSDCSDIPIIN-------ALQKGVRVIELDI 482
>TC216905
Length = 1359
Score = 27.7 bits (60), Expect = 6.9
Identities = 24/91 (26%), Positives = 37/91 (40%), Gaps = 14/91 (15%)
Frame = +3
Query: 51 LTTHNSYASRAANLSIDS------------KISSVMNQEDSITDQL--RNGVRGIMLDMH 96
L N A RAA++S+ K N E S + +NG + H
Sbjct: 360 LPVFNPLAGRAASISLSGPGGPFSFWNFSEKWKKQKNSESSNKKEYSPQNGD----VSKH 527
Query: 97 DYYGDIWLCRGPCTIFTAFQPAINVLREINT 127
+ G+ WL +G C I +++ NVL + T
Sbjct: 528 EALGNEWLAKGTCPIAKSYRAVSNVLPLVAT 620
>TC217319 similar to UP|Q8SNA3 (Q8SNA3) Bat3 protein (Fragment), partial (3%)
Length = 1053
Score = 27.3 bits (59), Expect = 9.0
Identities = 18/59 (30%), Positives = 34/59 (57%), Gaps = 1/59 (1%)
Frame = -2
Query: 25 QSRCTRVRTSSPISKVMELPFNHYSWLTTHNSYAS-RAANLSIDSKISSVMNQEDSITD 82
+S C+R+R +S PF+H + T H+SY + R N++ +S + QE+S+++
Sbjct: 719 RSPCSRLRKNSDAP-----PFHHQTVATLHHSYRNQREQNMNQES--LQLATQEESLSN 564
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.134 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,413,333
Number of Sequences: 63676
Number of extensions: 213221
Number of successful extensions: 998
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 995
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 997
length of query: 310
length of database: 12,639,632
effective HSP length: 97
effective length of query: 213
effective length of database: 6,463,060
effective search space: 1376631780
effective search space used: 1376631780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148528.6