BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148485.5 + phase: 0
(102 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU081086 weakly similar to GP|27817864|db OJ1081_B12.13 {Oryza s... 110 1e-25
TC215239 UP|H3_PEA (P02300) Histone H3, partial (39%) 32 0.038
TC224067 32 0.049
TC207505 27 1.2
TC208603 27 1.6
TC205459 homologue to UP|Q7XBE4 (Q7XBE4) Enolase , complete 27 1.6
TC230623 UP|Q9FRU2 (Q9FRU2) Nitrate transporter NRT1-5 (Fragment... 26 3.5
TC209385 homologue to UP|ARF5_ARATH (Q9ZPX1) Probable ADP-ribosy... 26 3.5
TC214893 UP|Q6RIB7 (Q6RIB7) Enolase , complete 25 4.6
TC212192 similar to UP|Q9SSM7 (Q9SSM7) F3N23.20 protein, partial... 25 4.6
TC205811 25 6.0
TC206353 25 6.0
TC209699 25 7.8
CD418258 similar to PIR|G86247|G862 protein T23J18.5 [imported] ... 25 7.8
TC234319 similar to UP|Q8L9P8 (Q8L9P8) RALF, partial (55%) 25 7.8
TC232690 weakly similar to UP|HIT_BACSU (O07513) Hit protein, pa... 25 7.8
TC230570 similar to UP|MLO6_ARATH (Q94KB7) MLO-like protein 6 (A... 25 7.8
TC205810 25 7.8
>BU081086 weakly similar to GP|27817864|db OJ1081_B12.13 {Oryza sativa
(japonica cultivar-group)}, partial (7%)
Length = 426
Score = 110 bits (275), Expect = 1e-25
Identities = 54/97 (55%), Positives = 68/97 (69%)
Frame = +2
Query: 1 MLLRNVDQASGLCNGTRLIIVSLGKNVICARVIGGAHAGEVVYIPRMNLIPSGANVSITF 60
MLLRN+DQ GLCN TRLII ++I A+++ G G VYIPR+ PS +
Sbjct: 134 MLLRNLDQNEGLCNSTRLIITMFADHIIEAKIMSGKGQGNTVYIPRLATSPSQSPWPFKL 313
Query: 61 ERRQFPLVLSFAMTINKSQGQTLTSVGLYLPRPVFTH 97
RR+FP+++S+AMTINKSQGQ L SVGLYLP PVF+H
Sbjct: 314 IRRKFPIIVSYAMTINKSQGQLLASVGLYLPTPVFSH 424
>TC215239 UP|H3_PEA (P02300) Histone H3, partial (39%)
Length = 340
Score = 32.3 bits (72), Expect = 0.038
Identities = 13/18 (72%), Positives = 16/18 (88%)
Frame = -3
Query: 65 FPLVLSFAMTINKSQGQT 82
FPL++ FAMT NKS+GQT
Sbjct: 326 FPLIVCFAMTTNKSEGQT 273
>TC224067
Length = 415
Score = 32.0 bits (71), Expect = 0.049
Identities = 19/43 (44%), Positives = 28/43 (64%), Gaps = 2/43 (4%)
Frame = -2
Query: 59 TFERRQFPLVLSFAMTINKSQGQTLTSV-GLYLPRPV-FTHGQ 99
+F+ + ++ + M INKS GQTL+ V G+ LPRP +HGQ
Sbjct: 390 SFKFQDVNSLVCYTMKINKSHGQTLSQVGGVLLPRPN**SHGQ 262
>TC207505
Length = 1191
Score = 27.3 bits (59), Expect = 1.2
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = -3
Query: 58 ITFERRQFPLVLSFAMTINKSQGQ 81
+ F + Q+PL+L+ +T NKSQGQ
Sbjct: 487 VLFPKPQYPLLLTN*ITQNKSQGQ 416
>TC208603
Length = 513
Score = 26.9 bits (58), Expect = 1.6
Identities = 11/23 (47%), Positives = 15/23 (64%)
Frame = -3
Query: 72 AMTINKSQGQTLTSVGLYLPRPV 94
A T +S+ L SV LYLP+P+
Sbjct: 373 AFTAERSRASMLASVDLYLPQPI 305
>TC205459 homologue to UP|Q7XBE4 (Q7XBE4) Enolase , complete
Length = 1667
Score = 26.9 bits (58), Expect = 1.6
Identities = 15/54 (27%), Positives = 24/54 (43%)
Frame = +2
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P GA+ + + I K GQ T+VG
Sbjct: 572 GGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVG 733
>TC230623 UP|Q9FRU2 (Q9FRU2) Nitrate transporter NRT1-5 (Fragment), complete
Length = 1873
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = +1
Query: 67 LVLSFAMTINKSQGQTLTSVGLYLP 91
L+ S + I + +G T++S+G++LP
Sbjct: 454 LINSIPIQIQEKKGSTVSSIGIFLP 528
>TC209385 homologue to UP|ARF5_ARATH (Q9ZPX1) Probable ADP-ribosylation
factor At2g18390, partial (88%)
Length = 958
Score = 25.8 bits (55), Expect = 3.5
Identities = 15/25 (60%), Positives = 15/25 (60%), Gaps = 3/25 (12%)
Frame = -3
Query: 77 KSQGQTLTSV---GLYLPRPVFTHG 98
KS QTLTSV YLP P F HG
Sbjct: 464 KSLKQTLTSVCATANYLPMPRFIHG 390
>TC214893 UP|Q6RIB7 (Q6RIB7) Enolase , complete
Length = 1920
Score = 25.4 bits (54), Expect = 4.6
Identities = 14/54 (25%), Positives = 24/54 (43%)
Frame = +3
Query: 34 GGAHAGEVVYIPRMNLIPSGANVSITFERRQFPLVLSFAMTINKSQGQTLTSVG 87
GG+HAG + + ++P GA+ + + + I K GQ +VG
Sbjct: 546 GGSHAGNKLAMQEFMVLPVGASSFKEAMKMGVEVYHNLKSVIKKKYGQDAVNVG 707
>TC212192 similar to UP|Q9SSM7 (Q9SSM7) F3N23.20 protein, partial (77%)
Length = 1027
Score = 25.4 bits (54), Expect = 4.6
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
Frame = +3
Query: 3 LRNVDQASGLCNGT---RLIIVSLGKNVICARVIGGAH 37
+R V SGL T RL I+ K+V+ RV+GG H
Sbjct: 405 IREVTVVSGLPASTSTERLEILDDDKHVLSFRVVGGEH 518
>TC205811
Length = 816
Score = 25.0 bits (53), Expect = 6.0
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 67 LVLSFAMTINKSQGQTLTSVGL 88
LVL F TINK+ LTS G+
Sbjct: 37 LVLEFTSTINKTMSVLLTSHGM 102
>TC206353
Length = 883
Score = 25.0 bits (53), Expect = 6.0
Identities = 8/24 (33%), Positives = 16/24 (66%)
Frame = -2
Query: 76 NKSQGQTLTSVGLYLPRPVFTHGQ 99
++ +G+T+ +G +LP P+ H Q
Sbjct: 534 HQGRGKTVDPLGFHLPPPLLAHSQ 463
>TC209699
Length = 978
Score = 24.6 bits (52), Expect = 7.8
Identities = 15/80 (18%), Positives = 34/80 (41%), Gaps = 12/80 (15%)
Frame = -2
Query: 12 LCNGTRLIIVSLGKNVICARVIGGAHAGEVVY------------IPRMNLIPSGANVSIT 59
LC G+ + +S+GK+++ R+I ++ I ++ + N +T
Sbjct: 944 LCQGSHISPLSIGKHILSLRIIRNTKLCNFLFQSHM*LEKARHEIIQLPISDKSLNYFVT 765
Query: 60 FERRQFPLVLSFAMTINKSQ 79
+ Q P++ + +SQ
Sbjct: 764 AHKLQKPIISRYLSADGQSQ 705
>CD418258 similar to PIR|G86247|G862 protein T23J18.5 [imported] -
Arabidopsis thaliana, partial (16%)
Length = 485
Score = 24.6 bits (52), Expect = 7.8
Identities = 10/30 (33%), Positives = 18/30 (59%)
Frame = -2
Query: 45 PRMNLIPSGANVSITFERRQFPLVLSFAMT 74
P N+ P G+ S+ F + +PL LS+ ++
Sbjct: 205 PNTNICPKGSPTSVFFFFKIYPLYLSWLIS 116
>TC234319 similar to UP|Q8L9P8 (Q8L9P8) RALF, partial (55%)
Length = 421
Score = 24.6 bits (52), Expect = 7.8
Identities = 10/24 (41%), Positives = 15/24 (61%)
Frame = -1
Query: 13 CNGTRLIIVSLGKNVICARVIGGA 36
CNGT +V +G + A V+GG+
Sbjct: 367 CNGTAATMVRVGLSARLAMVVGGS 296
>TC232690 weakly similar to UP|HIT_BACSU (O07513) Hit protein, partial (46%)
Length = 794
Score = 24.6 bits (52), Expect = 7.8
Identities = 15/49 (30%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Frame = +3
Query: 10 SGLCNGTRLIIVSLGKNVICAR----VIGGAHAGEVVYIPRMNLIPSGA 54
+ +C+ I ++ K C+ V GA AG+V+Y M++IP A
Sbjct: 357 AAMCSKVPFISNAIMKATGCSSFNLLVNNGAAAGQVIYHTHMHIIPRKA 503
>TC230570 similar to UP|MLO6_ARATH (Q94KB7) MLO-like protein 6 (AtMlo6),
partial (24%)
Length = 957
Score = 24.6 bits (52), Expect = 7.8
Identities = 12/39 (30%), Positives = 23/39 (58%)
Frame = -1
Query: 26 NVICARVIGGAHAGEVVYIPRMNLIPSGANVSITFERRQ 64
+V+CA + GA++ + + R + S N S++ ERR+
Sbjct: 351 SVLCASSLSGANSATLPLLYR*ISLASLLNPSVSLERRE 235
>TC205810
Length = 761
Score = 24.6 bits (52), Expect = 7.8
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +2
Query: 67 LVLSFAMTINKSQGQTLTSVGL 88
LVL F TINK+ LTS G+
Sbjct: 422 LVLEFTSTINKAMSILLTSHGM 487
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.325 0.141 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,207,090
Number of Sequences: 63676
Number of extensions: 47574
Number of successful extensions: 274
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 274
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 274
length of query: 102
length of database: 12,639,632
effective HSP length: 78
effective length of query: 24
effective length of database: 7,672,904
effective search space: 184149696
effective search space used: 184149696
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148485.5