BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148469.4 - phase: 0 /pseudo
(312 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG790501 94 1e-19
TC217630 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g2... 75 3e-14
TC209676 similar to UP|Q9D2D1 (Q9D2D1) Mus musculus 11 days preg... 75 3e-14
TC217627 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g2... 74 8e-14
TC206452 similar to UP|CBPY_YEAST (P00729) Carboxypeptidase Y pr... 71 7e-13
TC204504 similar to UP|Q9LY68 (Q9LY68) Serin carboxypeptidase-li... 70 1e-12
TC222981 similar to UP|Q9LY68 (Q9LY68) Serin carboxypeptidase-li... 70 1e-12
TC214484 similar to UP|Q9M513 (Q9M513) Wound-inducible carboxype... 67 1e-11
BE331042 66 2e-11
BQ272830 66 2e-11
CA853190 63 1e-10
TC217629 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g2... 62 3e-10
CO984402 60 1e-09
BG362620 53 2e-07
TC232338 similar to UP|Q9XH61 (Q9XH61) Serine carboxypeptidase, ... 50 2e-06
AW733433 35 6e-06
BG726925 46 2e-05
AW186053 46 2e-05
BE440580 42 3e-04
CA852135 similar to GP|10177009|db serine carboxypeptidase II-li... 39 0.003
>BG790501
Length = 421
Score = 93.6 bits (231), Expect = 1e-19
Identities = 44/60 (73%), Positives = 51/60 (84%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+LYLESPAGVGFSYS+N S Y +VTDE TARD L+FLQ W T+F +Y N+DFFITGESYA
Sbjct: 222 VLYLESPAGVGFSYSSNASFYTLVTDEITARDNLVFLQRWFTEFPEYSNNDFFITGESYA 401
>TC217630 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g28110
{Arabidopsis thaliana;} , partial (33%)
Length = 684
Score = 75.5 bits (184), Expect = 3e-14
Identities = 68/204 (33%), Positives = 95/204 (46%), Gaps = 5/204 (2%)
Frame = +1
Query: 108 VPFVIECFISLLLRLVHLLILLISLKTFASHLNFSWFMR-LQWKLERREMYVWKVKPPHT 166
V FV + LLRL + ++SL H S M ++ K RR M+VW +K HT
Sbjct: 1 VTFVQK*MXXCLLRLATI*TNMMSLXMCVFHQQISXLMS*IKCKRHRR*MFVWMIKQSHT 180
Query: 167 *IEAKCKKQFMQSW*ESPSGLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRI 226
* C++ M S ES +G A S T I R + +L+ LL P E W VE +I
Sbjct: 181 *TGKMCRRLSMLSLXESQNGQLAAEFSIMTVGI*RYQPFLF*EHLLIPTSEFWYTVEIKI 360
Query: 227 RSFH*LGQSLC*RGWPR----ISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERV 282
+ H + L * PR I W + PGL ++R DG K TS + P EE +
Sbjct: 361 QLSHYWDRDLW*MD*PRNLD*IQQWHI----EPGLRENR*QDGHKYTETSYHMPP*EEHL 528
Query: 283 MQPQLLNQGGPSGCFRVS*KQNHF 306
M+ LN S + *K++H+
Sbjct: 529 MKLPSLNHRDHSCYLKHF*KESHY 600
>TC209676 similar to UP|Q9D2D1 (Q9D2D1) Mus musculus 11 days pregnant adult
female ovary and uterus cDNA, RIKEN full-length enriched
library, clone:5031400F07 product:protective protein for
beta-galactosidase, full insert sequence, partial (12%)
Length = 642
Score = 75.5 bits (184), Expect = 3e-14
Identities = 33/60 (55%), Positives = 43/60 (71%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+L+SPAGVGFSYS TSD + D+RTA D FL W +F +Y++ DF+I GESYA
Sbjct: 461 ILFLDSPAGVGFSYSNTTSDLYTAXDQRTAEDAYTFLVNWFERFPQYKHRDFYIAGESYA 640
>TC217627 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g28110
{Arabidopsis thaliana;} , partial (37%)
Length = 910
Score = 73.9 bits (180), Expect = 8e-14
Identities = 73/220 (33%), Positives = 103/220 (46%), Gaps = 5/220 (2%)
Frame = +2
Query: 92 SATTPKL*VKT*MGHLVPFVIECFISLLLRLVHLLILLISLKTFASHLNFSWFMR-LQWK 150
SAT P+L K MG L FV + ++RL + + ++SL F H + M + K
Sbjct: 32 SATFPQLGDKCKMGILEGFVEKQISYWIVRLATM*MNMMSLLMFVCHQ*INRLMC*INCK 211
Query: 151 LERREMYVWKVKPPHT*IEAKCKKQFMQSW*ESPSGLHVAMSSSTTGRILRIRLYLYWGD 210
RR M+V +K HT* K KK M ESP+G A RI + + +L+ G
Sbjct: 212 RHRR*MFVLVIKQLHT*TLKKSKKLCMLILLESPNGQLAAQFCIMIIRI*KYQQFLF*GH 391
Query: 211 LLGPGYELWCIVETRIRSFH*LGQSLC*RGWPR----ISVWTLVTTTGPGLMDHRLLDGL 266
LL C VE +I+ FH + L PR I W + PGL ++R DG
Sbjct: 392 LLTLASGS*CTVEIKIQCFHY*VRDL*LTD*PRKLDLIQPWPI----EPGLRENRWQDGH 559
Query: 267 KLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRVS*KQNHF 306
K M TS + P EE +M+ LN SG + K++H+
Sbjct: 560 KYMGTSYHMPP*EEHLMKLPFLNHRDHSGYLKPFWKESHY 679
>TC206452 similar to UP|CBPY_YEAST (P00729) Carboxypeptidase Y precursor
(Carboxypeptidase YSCY) , partial (5%)
Length = 1330
Score = 70.9 bits (172), Expect = 7e-13
Identities = 31/65 (47%), Positives = 45/65 (68%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+L+SPAGVGFSYS T+D + D++TA D FL W +F +Y++ +F+I GESYA
Sbjct: 1 VLFLDSPAGVGFSYSNKTTDLYTFGDQKTAEDAYTFLVNWFERFPQYKHREFYIAGESYA 180
Query: 61 VWNLP 65
+P
Sbjct: 181GHYVP 195
>TC204504 similar to UP|Q9LY68 (Q9LY68) Serin carboxypeptidase-like protein,
partial (54%)
Length = 1672
Score = 70.1 bits (170), Expect = 1e-12
Identities = 32/65 (49%), Positives = 45/65 (69%)
Frame = +3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L+LESPAGVGFSYS + DY D++TA D +FL W+ ++ +Y+ DF+I GESYA
Sbjct: 630 VLFLESPAGVGFSYSNKSKDYDTNGDKKTAADNYLFLVNWLERYPEYKERDFYIAGESYA 809
Query: 61 VWNLP 65
+P
Sbjct: 810 GHYVP 824
>TC222981 similar to UP|Q9LY68 (Q9LY68) Serin carboxypeptidase-like protein,
partial (30%)
Length = 637
Score = 70.1 bits (170), Expect = 1e-12
Identities = 31/57 (54%), Positives = 42/57 (73%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGE 57
+L+LESPAGVGFSYS TSDY D+ TA+D +FL W+ +F +Y+ +F+ITGE
Sbjct: 467 VLFLESPAGVGFSYSNTTSDYDRSGDKSTAKDAYVFLINWLERFPEYKTREFYITGE 637
>TC214484 similar to UP|Q9M513 (Q9M513) Wound-inducible carboxypeptidase,
partial (84%)
Length = 2125
Score = 66.6 bits (161), Expect = 1e-11
Identities = 39/89 (43%), Positives = 51/89 (56%), Gaps = 11/89 (12%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
++YL+SPAGVGFSYS NTS Y D TA D +FL W +F ++Q + F+I GESYA
Sbjct: 422 VIYLDSPAGVGFSYSKNTSKY-ATGDLETASDTHLFLLKWFQQFPEFQANPFYIAGESYA 598
Query: 61 VWNLPLI-----------TIPGLNFYGPM 78
+P + T P +NF G M
Sbjct: 599 GVYVPTLAFEVAKGIRSGTKPVINFKGYM 685
>BE331042
Length = 324
Score = 65.9 bits (159), Expect = 2e-11
Identities = 30/64 (46%), Positives = 44/64 (67%)
Frame = +1
Query: 5 ESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYAVWNL 64
+SPAGVGFSYS + DY D++TA D +FL W+ ++ +Y++ DF+I GESYA +
Sbjct: 106 KSPAGVGFSYSNKSKDYDNNGDKKTAADNYLFLVNWLERYPEYKDRDFYIAGESYAGHYV 285
Query: 65 PLIT 68
P +T
Sbjct: 286 PQLT 297
>BQ272830
Length = 423
Score = 65.9 bits (159), Expect = 2e-11
Identities = 33/68 (48%), Positives = 44/68 (64%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQNSDFFITGESYA 60
+L++ESPAGVG+SYS TSDY D TA D+ +F+ W KF Y + F+TGESYA
Sbjct: 35 LLFVESPAGVGWSYSNTTSDYNS-GDASTANDMYLFMLKWYEKFPSYITRELFLTGESYA 211
Query: 61 VWNLPLIT 68
+P +T
Sbjct: 212 GHYIPQLT 235
>CA853190
Length = 588
Score = 63.2 bits (152), Expect = 1e-10
Identities = 29/49 (59%), Positives = 35/49 (71%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQGWVTKFQKYQN 49
MLYLE+P GVGFSY+ +S Y V DE TARD LIFL W KF +Y++
Sbjct: 427 MLYLETPVGVGFSYAKGSSSYMTVNDEATARDNLIFLLRWFNKFPQYRS 573
>TC217629 weakly similar to GB|AAP49525.1|31376397|BT008763 At1g28110
{Arabidopsis thaliana;} , partial (36%)
Length = 694
Score = 62.4 bits (150), Expect = 3e-10
Identities = 60/185 (32%), Positives = 86/185 (46%), Gaps = 5/185 (2%)
Frame = +3
Query: 127 ILLISLKTFASHLNFSWFMR-LQWKLERREMYVWKVKPPHT*IEAKCKKQFMQSW*ESPS 185
I ++SL F H + M + K RR M+V +K PHT* E K KK +M ESP+
Sbjct: 9 INMMSLSMFVCHQ*INRLMC*INCKRHRR*MFVLVIKQPHT*TENKSKKLYMLILLESPN 188
Query: 186 GLHVAMSSSTTGRILRIRLYLYWGDLLGPGYELWCIVETRIRSFH*LGQSLC*RGWPR-- 243
G A T RI + + +L+ G LL VE +I+ FH* + L * PR
Sbjct: 189 GQLAAQFCIMTIRI*KYQQFLF*GHLLSLASRF*YTVEIKIQ*FH**VRDL**MD*PRKL 368
Query: 244 --ISVWTLVTTTGPGLMDHRLLDGLKLMVTSSPLQPLEERVMQPQLLNQGGPSGCFRVS* 301
I W + PGL ++R DG + M TS + E +M+ LN +
Sbjct: 369 DLIQQWPI----EPGLRENRWQDGQRYMGTSYHMPL*EVHLMKLPFLNHRDHCCYLKHFW 536
Query: 302 KQNHF 306
K++H+
Sbjct: 537 KESHY 551
>CO984402
Length = 737
Score = 60.5 bits (145), Expect = 1e-09
Identities = 54/156 (34%), Positives = 68/156 (42%), Gaps = 3/156 (1%)
Frame = -2
Query: 156 MYVWKVKPPHT*IEAKCKKQFMQSW*ESPSGLHVAMS---SSTTGRILRIRLYLYWGDLL 212
MYVWK+K * C + M E GL AMS S T R + RL WG
Sbjct: 601 MYVWKMKQSII*TGKMCSQLCMHILLEFKDGLLAAMSWIMSCVTWRYQQSRL---WGS*S 431
Query: 213 GPGYELWCIVETRIRSFH*LGQSLC*RGWPRISVWTLVTTTGPGLMDHRLLDGLKLMVTS 272
Y+ W V RI+ H*L W + T + TG GL R + GLK MVTS
Sbjct: 430 RREYQYWFTVVIRIQLSH*LEVEH*YINWQKNLD*TPLCHTGCGLRSSRWVGGLKFMVTS 251
Query: 273 SPLQPLEERVMQPQLLNQGGPSGCFRVS*KQNHFQE 308
L P EE M+ + C S*K++H+ +
Sbjct: 250 FHLPPSEEHHMKLHFHSLRDHLCCSSPS*KEDHYHK 143
>BG362620
Length = 507
Score = 53.1 bits (126), Expect = 2e-07
Identities = 26/38 (68%), Positives = 30/38 (78%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERTARDVLIFLQ 38
+LYLESPAGVGFSYS N S Y + DE TARD L+FL+
Sbjct: 392 ILYLESPAGVGFSYSLNLSFYKTLNDEITARDSLVFLR 505
>TC232338 similar to UP|Q9XH61 (Q9XH61) Serine carboxypeptidase, partial
(57%)
Length = 1257
Score = 49.7 bits (117), Expect = 2e-06
Identities = 27/68 (39%), Positives = 43/68 (62%), Gaps = 1/68 (1%)
Frame = +1
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDERT-ARDVLIFLQGWVTKFQKYQNSDFFITGESY 59
+L+++ P G GFSYS++ SD + DE + + D+ FLQ + K+ +DF+ITGESY
Sbjct: 433 ILFVDQPTGTGFSYSSDDSD--IRHDEASISNDLYDFLQEFFKAHPKFVKNDFYITGESY 606
Query: 60 AVWNLPLI 67
A +P +
Sbjct: 607 AGHYIPAL 630
>AW733433
Length = 417
Score = 35.4 bits (80), Expect(2) = 6e-06
Identities = 13/32 (40%), Positives = 23/32 (71%)
Frame = -2
Query: 28 RTARDVLIFLQGWVTKFQKYQNSDFFITGESY 59
++A D FL W+ +F ++++ +FFI+GESY
Sbjct: 134 QSAEDTYNFLVNWLQRFPQFKSREFFISGESY 39
Score = 32.0 bits (71), Expect(2) = 6e-06
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = -3
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTD 26
+L++ESP GVGF Y+ +SD+ ++ D
Sbjct: 301 LLFVESPVGVGFFYTNTSSDFTILED 224
>BG726925
Length = 413
Score = 46.2 bits (108), Expect = 2e-05
Identities = 20/36 (55%), Positives = 25/36 (68%)
Frame = +1
Query: 30 ARDVLIFLQGWVTKFQKYQNSDFFITGESYAVWNLP 65
ARD L+FLQ W KF +Y+N DF+I GESY +P
Sbjct: 19 ARDSLVFLQRWFAKFPEYKNRDFYIMGESYGGHYVP 126
>AW186053
Length = 423
Score = 45.8 bits (107), Expect = 2e-05
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = +2
Query: 1 MLYLESPAGVGFSYSANTSDYFMVTDE 27
+LYLESPAGVGFSYS+N S Y +VTDE
Sbjct: 338 VLYLESPAGVGFSYSSNESFYALVTDE 418
>BE440580
Length = 298
Score = 42.4 bits (98), Expect = 3e-04
Identities = 33/87 (37%), Positives = 43/87 (48%)
Frame = +3
Query: 147 LQWKLERREMYVWKVKPPHT*IEAKCKKQFMQSW*ESPSGLHVAMSSSTTGRILRIRLYL 206
L K R M+V K+K *I CKK + S * S G AM + T RI R + +L
Sbjct: 15 LNCKRVPR*MFVSKMKLSLL*IGKMCKKLSLLSS*GSRHGQLAAMFLNMTCRI*RFQPFL 194
Query: 207 YWGDLLGPGYELWCIVETRIRSFH*LG 233
++ LL WC V I++ H*LG
Sbjct: 195 FYVHLLSQVSGCWCTVHIMIQTSH*LG 275
>CA852135 similar to GP|10177009|db serine carboxypeptidase II-like
{Arabidopsis thaliana}, partial (45%)
Length = 692
Score = 38.9 bits (89), Expect = 0.003
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +2
Query: 32 DVLIFLQGWVTKFQKYQNSDFFITGESYAVWNLP 65
D+L+FL W KF Y++ + F+TGESYA +P
Sbjct: 35 DMLLFLLKWYQKFPSYRSRELFLTGESYAGHYIP 136
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.340 0.150 0.525
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,290,596
Number of Sequences: 63676
Number of extensions: 282207
Number of successful extensions: 2052
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 2036
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2045
length of query: 312
length of database: 12,639,632
effective HSP length: 97
effective length of query: 215
effective length of database: 6,463,060
effective search space: 1389557900
effective search space used: 1389557900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148469.4