BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148404.5 - phase: 0
(192 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM307869 weakly similar to GP|20145851|emb auxin response factor... 117 3e-27
CO981049 71 4e-13
AW703646 62 1e-10
TC229119 39 0.001
CD414165 33 0.081
AW781164 similar to GP|18146862|dbj| pollen-specific calcium-bin... 32 0.14
AW279106 31 0.31
TC229118 similar to UP|Q6R2U8 (Q6R2U8) Reduced vernalization res... 30 0.53
AW186513 28 2.0
BM521715 28 2.0
TC206416 28 2.0
TC229117 similar to UP|Q8L3W1 (Q8L3W1) Reduced vernalization res... 28 2.6
TC206042 homologue to UP|O81278 (O81278) Nodule-enhanced malate ... 28 3.4
CK606258 28 3.4
TC206589 similar to UP|Q6NMZ3 (Q6NMZ3) At4g34750, partial (39%) 27 4.5
AW311432 similar to SP|P49692|RL7A 60S ribosomal protein L7a. [M... 27 4.5
TC224404 homologue to UP|Q9M728 (Q9M728) Viroid symptom modulati... 27 4.5
TC210940 homologue to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deace... 27 4.5
TC227517 similar to UP|RL7A_ARATH (P49692) 60S ribosomal protein... 27 5.8
>BM307869 weakly similar to GP|20145851|emb auxin response factor 36
{Arabidopsis thaliana}, partial (8%)
Length = 437
Score = 117 bits (294), Expect = 3e-27
Identities = 54/74 (72%), Positives = 59/74 (78%)
Frame = +2
Query: 13 LQALPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKAND 72
L ALPK FSDNL KKLPENVTLKGP GV WNI +TTRD +YF GW+QF+ DH LK ND
Sbjct: 215 LLALPKAFSDNLKKKLPENVTLKGPGGVVWNIGMTTRDDTLYFAHGWEQFVKDHCLKEND 394
Query: 73 FLVCKYNGESHFEV 86
FLV KYNGES F+V
Sbjct: 395 FLVFKYNGESQFDV 436
>CO981049
Length = 665
Score = 70.9 bits (172), Expect = 4e-13
Identities = 36/83 (43%), Positives = 46/83 (55%)
Frame = -2
Query: 26 KKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKANDFLVCKYNGESHFE 85
K L + LK P+G W + L RDG V+F +GW++F+ HSL LV KY+G HF
Sbjct: 652 KHLSNTMFLKLPNGAEWRVNLEKRDGRVWFQEGWKKFVEHHSLAHGHLLVFKYDGTFHFH 473
Query: 86 VLIFDGESFCEKEASYFVEKCGH 108
VLIFD + E Y V K H
Sbjct: 472 VLIFDPSA---NEIDYPVNKANH 413
>AW703646
Length = 422
Score = 62.4 bits (150), Expect = 1e-10
Identities = 29/75 (38%), Positives = 42/75 (55%)
Frame = +2
Query: 16 LPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKANDFLV 75
+P F K+L + LK P+G W + L RDG ++F GW++F HSL LV
Sbjct: 41 IPNKFVKKYGKRLQNTLFLKTPNGAEWKMILKKRDGKIWFQKGWKEFAEYHSLAHGHLLV 220
Query: 76 CKYNGESHFEVLIFD 90
+++ SHF+V IFD
Sbjct: 221 FRWDVTSHFQVHIFD 265
>TC229119
Length = 455
Score = 38.9 bits (89), Expect = 0.001
Identities = 18/36 (50%), Positives = 22/36 (61%)
Frame = +2
Query: 55 FVDGWQQFMNDHSLKANDFLVCKYNGESHFEVLIFD 90
FVDGWQ F+ +S+ FLV Y G S F V IF+
Sbjct: 5 FVDGWQDFVQHYSIGVGYFLVFMYEGNSSFIVHIFN 112
>CD414165
Length = 623
Score = 33.1 bits (74), Expect = 0.081
Identities = 19/71 (26%), Positives = 30/71 (41%), Gaps = 7/71 (9%)
Frame = -1
Query: 26 KKLPE---NVTLKGPSGVTWNIRLTT----RDGFVYFVDGWQQFMNDHSLKANDFLVCKY 78
K +PE + L G W + +T + F GW +F+ D+ L D + +
Sbjct: 362 KYIPEVVKQIILWNSEGKFWEVEVTCLRYPNKRYTRFTTGWGKFVRDNKLMPGDTCIFEL 183
Query: 79 NGESHFEVLIF 89
E+H V IF
Sbjct: 182 EDENHMSVHIF 150
>AW781164 similar to GP|18146862|dbj| pollen-specific calcium-binding protein
{Nicotiana tabacum}, partial (98%)
Length = 250
Score = 32.3 bits (72), Expect = 0.14
Identities = 16/39 (41%), Positives = 22/39 (56%)
Frame = +2
Query: 107 GHAQTAQGGSNASETNNSIEEVDTDSNGGDSPEQFTDDA 145
G A A G A E +EE+DTD +G S ++FT+ A
Sbjct: 92 GEALKALGSVTAEEVKRMMEEIDTDGDGYISYQEFTEFA 208
>AW279106
Length = 441
Score = 31.2 bits (69), Expect = 0.31
Identities = 24/69 (34%), Positives = 32/69 (45%), Gaps = 3/69 (4%)
Frame = +3
Query: 11 LLLQALPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYF--VDGWQQFMNDHSL 68
+L QA+P T +L+ + PE KGP+ V N FV F VD W M H +
Sbjct: 228 VLRQAMPDTPIISLSPRHPEKYLKKGPAYVDGNCTYYAGKDFVDFGSVD-WPNVMKKHGI 404
Query: 69 K-ANDFLVC 76
N L+C
Sbjct: 405 SDLNRVLIC 431
>TC229118 similar to UP|Q6R2U8 (Q6R2U8) Reduced vernalization response 1,
partial (17%)
Length = 470
Score = 30.4 bits (67), Expect = 0.53
Identities = 16/60 (26%), Positives = 28/60 (46%)
Frame = +3
Query: 16 LPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKANDFLV 75
LP TF++ + + L+ +G W++R R G GW +F +++L D V
Sbjct: 15 LPSTFAEKNLNGVSGFIKLQLSNGRQWSVRCLYRGGRAKLSQGWFEFTVENNLGEGDVCV 194
>AW186513
Length = 439
Score = 28.5 bits (62), Expect = 2.0
Identities = 14/58 (24%), Positives = 30/58 (51%)
Frame = +3
Query: 32 VTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKANDFLVCKYNGESHFEVLIF 89
V ++ P+G ++T +G D W Q++ +++L A D ++ + + H +IF
Sbjct: 228 VPVQRPNGPRNQWQITVHNGLPSVADPWFQYLCENNLMAGDEVMFFFRFDKHVWEVIF 401
>BM521715
Length = 277
Score = 28.5 bits (62), Expect = 2.0
Identities = 14/43 (32%), Positives = 18/43 (41%)
Frame = +2
Query: 16 LPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDG 58
LPK F+ + V LK G W I T G ++F G
Sbjct: 149 LPKKFTRKYGDGMSNPVFLKPADGTEWKIHYTKHGGEIWFQKG 277
>TC206416
Length = 1101
Score = 28.5 bits (62), Expect = 2.0
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 4/47 (8%)
Frame = +3
Query: 48 TRDGFVYFVDGWQQFMNDHSLKANDFLVCK----YNGESHFEVLIFD 90
T++G + DG F N+H+L +DF + GES++E FD
Sbjct: 267 TKEGLMK--DGKSSFANNHALPKSDFAIANGIGPPKGESNYEAAEFD 401
>TC229117 similar to UP|Q8L3W1 (Q8L3W1) Reduced vernalization response 1,
partial (38%)
Length = 904
Score = 28.1 bits (61), Expect = 2.6
Identities = 15/60 (25%), Positives = 27/60 (45%)
Frame = +3
Query: 16 LPKTFSDNLNKKLPENVTLKGPSGVTWNIRLTTRDGFVYFVDGWQQFMNDHSLKANDFLV 75
LP F++ + + L+ +G W++R R G GW +F +++L D V
Sbjct: 426 LPSCFAEKNLNGVSGFIKLQLSNGRQWSVRCLYRGGRAKLSQGWFEFTVENNLGEGDVCV 605
>TC206042 homologue to UP|O81278 (O81278) Nodule-enhanced malate
dehydrogenase , partial (53%)
Length = 751
Score = 27.7 bits (60), Expect = 3.4
Identities = 13/29 (44%), Positives = 16/29 (54%)
Frame = +2
Query: 95 CEKEASYFVEKCGHAQTAQGGSNASETNN 123
CE E+S+F K G A A S A + NN
Sbjct: 257 CESESSFFGNKTGAALRASFASKAQKENN 343
>CK606258
Length = 627
Score = 27.7 bits (60), Expect = 3.4
Identities = 17/55 (30%), Positives = 27/55 (48%), Gaps = 5/55 (9%)
Frame = +2
Query: 92 ESFC--EKEASYFV---EKCGHAQTAQGGSNASETNNSIEEVDTDSNGGDSPEQF 141
E+FC +K++ F+ E ++ +G E + I EVD D NG E+F
Sbjct: 140 EAFCLIDKDSDGFITVDELATIIRSLEGNPTKEEIQDMISEVDIDGNGSIDFEEF 304
>TC206589 similar to UP|Q6NMZ3 (Q6NMZ3) At4g34750, partial (39%)
Length = 686
Score = 27.3 bits (59), Expect = 4.5
Identities = 19/49 (38%), Positives = 28/49 (56%), Gaps = 1/49 (2%)
Frame = -2
Query: 132 SNGGDSPE-QFTDDAVPKTTAIQSPFIPTGKRTKRRRRSPKAAANWGAR 179
SNG DSP + T V K+++ + P TG+ T RRR S + ++ AR
Sbjct: 445 SNGHDSPPTKSTWQRV*KSSSEEKPGRGTGRATTRRRCSKRDSSQGIAR 299
>AW311432 similar to SP|P49692|RL7A 60S ribosomal protein L7a. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (51%)
Length = 446
Score = 27.3 bits (59), Expect = 4.5
Identities = 20/68 (29%), Positives = 28/68 (40%), Gaps = 4/68 (5%)
Frame = +2
Query: 105 KCGHAQTAQGGSNASETNNSIEEVDTDSNGGDSPEQFTDDAVPKTTAIQSPFIP----TG 160
+C + T + + T S + S G S T P T Q F+ TG
Sbjct: 140 RCRRSGT*RAS*SGRRTFKSNARSASSSRGSRSLRLSTSSPKPSTKISQQIFLKCC*STG 319
Query: 161 KRTKRRRR 168
+RT+RRRR
Sbjct: 320 RRTRRRRR 343
>TC224404 homologue to UP|Q9M728 (Q9M728) Viroid symptom modulation protein,
partial (40%)
Length = 829
Score = 27.3 bits (59), Expect = 4.5
Identities = 27/102 (26%), Positives = 38/102 (36%)
Frame = +1
Query: 68 LKANDFLVCKYNGESHFEVLIFDGESFCEKEASYFVEKCGHAQTAQGGSNASETNNSIEE 127
LK + LV E H + FD C + HA + GS NN +
Sbjct: 1 LKPENLLV---RDEGHIMLSDFDLSLRCSVNPTLVKSSSAHASNSSSGS-----NNDVGS 156
Query: 128 VDTDSNGGDSPEQFTDDAVPKTTAIQSPFIPTGKRTKRRRRS 169
+ TD D AV TT + S F P +K+ R++
Sbjct: 157 ILTD-----------DQAVQSTTQVSS-FFPRILPSKKNRKA 246
>TC210940 homologue to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (48%)
Length = 510
Score = 27.3 bits (59), Expect = 4.5
Identities = 21/55 (38%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
Frame = +1
Query: 86 VLIFDGE-SFCEKEASYFVEKCGHAQTAQGGSNASETNNSIEEVDTDSNGGDSPE 139
VL D E S K AS F CG+ + GG+NAS+ ++S E+ + G D+ E
Sbjct: 295 VLDSDSELSHTSKSASVFF--CGY-RVLTGGNNASDFSDSEEDEELALEGQDNDE 450
>TC227517 similar to UP|RL7A_ARATH (P49692) 60S ribosomal protein L7a,
complete
Length = 1081
Score = 26.9 bits (58), Expect = 5.8
Identities = 20/69 (28%), Positives = 30/69 (42%), Gaps = 4/69 (5%)
Frame = +1
Query: 105 KCGHAQTAQGGSNASETNNSIEEVDTDSNGGDSPEQFTDDAVPKTTAIQSPFI----PTG 160
+C + T +G + T+ S + S G S T P T ++ TG
Sbjct: 160 RCRRSGT*RGS*SGRRTSKSNARSASSSRGSRSLLHSTSSPKP*TRISPRIYLRCCSSTG 339
Query: 161 KRTKRRRRS 169
+RT+RRRRS
Sbjct: 340 RRTRRRRRS 366
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.317 0.134 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,757,699
Number of Sequences: 63676
Number of extensions: 131101
Number of successful extensions: 720
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 716
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 718
length of query: 192
length of database: 12,639,632
effective HSP length: 92
effective length of query: 100
effective length of database: 6,781,440
effective search space: 678144000
effective search space used: 678144000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC148404.5