BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148360.3 - phase: 0
(301 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC221368 38 0.005
TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - A... 38 0.005
TC224760 similar to UP|O04692 (O04692) DNA-binding protein, part... 38 0.005
TC224744 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glyco... 38 0.006
TC227389 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacety... 37 0.008
TC203614 UP|Q94LX2 (Q94LX2) Beta-conglycinin alpha subunit, comp... 37 0.014
TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Pro... 37 0.014
TC224625 similar to UP|O04692 (O04692) DNA-binding protein, part... 37 0.014
TC219508 homologue to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deace... 36 0.024
TC227390 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacety... 35 0.032
TC218919 homologue to UP|Q39892 (Q39892) Nucleosome assembly pro... 35 0.054
TC204249 UP|Q9SB11 (Q9SB11) Glycinin, complete 34 0.071
TC203635 similar to PIR|T46225|T46225 alpha NAC-like protein - A... 34 0.092
TC215203 similar to UP|Q70Z19 (Q70Z19) Nucleosome assembly prote... 34 0.092
TC223409 similar to UP|Q6T4P5 (Q6T4P5) Plasticity related gene 2... 34 0.092
TC204254 UP|GLC4_SOYBN (P02858) Glycinin G4 precursor [Contains:... 34 0.092
BF597314 similar to PIR|S20007|S20 beta-conglycinin alpha chain ... 34 0.092
TC215201 similar to UP|Q70Z19 (Q70Z19) Nucleosome assembly prote... 33 0.12
TC224564 similar to PIR|T51159|T51159 HMG protein [imported] - A... 33 0.12
TC221531 similar to UP|Q84N48 (Q84N48) CRS2-associated factor 2,... 33 0.16
>TC221368
Length = 629
Score = 38.1 bits (87), Expect = 0.005
Identities = 22/112 (19%), Positives = 53/112 (46%), Gaps = 7/112 (6%)
Frame = +1
Query: 58 KKAEISPKKISEYQIIEIAENVCNLKKVEADWILRIDI-------VEKADRLELEEHDSE 110
++ ++ ++SE +++E+ + +C + D+ L+ID V+ D L + ++
Sbjct: 67 REGKLIDYRVSELRVVELLDGLCEKMQ---DYTLKIDTNTYEWFKVDSWDNLTTNKQEAR 237
Query: 111 GQCNSECKTVERACQEVMGYSDTDVAEYLYSSKPDIDSLTNYLCKDLSKACN 162
K + C ++ ++ + AE + + ++ LC+DLSK C+
Sbjct: 238 AYS----KDISSYCGRLLEETEDEFAELIKKGSVKVGGVSKVLCQDLSKHCS 381
>TC224567 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (71%)
Length = 876
Score = 38.1 bits (87), Expect = 0.005
Identities = 23/70 (32%), Positives = 39/70 (54%), Gaps = 10/70 (14%)
Frame = +2
Query: 177 PFVAKSSK-EAEMEKLLKSMEGMPGAPG---------MKMYSRDDLMKKNFGAENEDDDE 226
P+ AK++K +AE EKL+K+ E + ++ DD + E+EDD+E
Sbjct: 431 PYEAKAAKRKAEYEKLIKAYEKKQASSADDDESDKSKSEVNDEDDASGEEDHQEDEDDEE 610
Query: 227 DEDEEEDEAD 236
+ED+E+D+ D
Sbjct: 611 EEDDEDDD*D 640
>TC224760 similar to UP|O04692 (O04692) DNA-binding protein, partial (73%)
Length = 654
Score = 38.1 bits (87), Expect = 0.005
Identities = 23/70 (32%), Positives = 39/70 (54%), Gaps = 10/70 (14%)
Frame = +3
Query: 177 PFVAKSSK-EAEMEKLLKSMEGMPGAPG---------MKMYSRDDLMKKNFGAENEDDDE 226
P+ AK++K +AE EKL+K+ E + ++ DD + E+EDD+E
Sbjct: 249 PYEAKAAKRKAEYEKLIKAYEKKQASSADDDESDKSKSEVNDEDDASGEEDHQEDEDDEE 428
Query: 227 DEDEEEDEAD 236
+ED+E+D+ D
Sbjct: 429 EEDDEDDD*D 458
>TC224744 similar to UP|Q09085 (Q09085) Hydroxyproline-rich glycoprotein
(HRGP) (Fragment), partial (30%)
Length = 592
Score = 37.7 bits (86), Expect = 0.006
Identities = 25/72 (34%), Positives = 36/72 (49%)
Frame = +1
Query: 165 PPPVPKDRTPGEPFVAKSSKEAEMEKLLKSMEGMPGAPGMKMYSRDDLMKKNFGAENEDD 224
PPP PK + P E AK ++AE EKL+K+ + + S DD +E D+
Sbjct: 397 PPPSPKPKPPYEAKAAK--RKAEYEKLIKAYDKKQAS------SADDEESDKSKSEVNDE 552
Query: 225 DEDEDEEEDEAD 236
D+ EEE+ D
Sbjct: 553 DDASGEEEEGDD 588
>TC227389 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (63%)
Length = 1144
Score = 37.4 bits (85), Expect = 0.008
Identities = 42/150 (28%), Positives = 66/150 (44%), Gaps = 12/150 (8%)
Frame = +3
Query: 162 NTKPPPVPKD-RTP-GEPFVAKSSKEAEMEKLLKSMEGMPGAPGMKMYSRDDLMKKNFGA 219
N KP +D + P + VAK+S A+ K+++ + + S DD +FG+
Sbjct: 429 NGKPETKAEDLKVPESKKAVAKASGSAKQVKVVEPKKDN------EEDSDDDSDDDDFGS 590
Query: 220 ENED--------DDEDEDEEEDEADFPSK--LGKILKSKENEKGDWKQKIRKGIVDTSTT 269
+E+ DDE DE++DE P K LGK ++ K K K T
Sbjct: 591 SDEEMEDADSDSDDESGDEDDDEETPPKKVDLGKKRPNESASKTPVSSKKAKNATPEKTD 770
Query: 270 LKKHATKVSNHIQRWWKGKKTTSSKKNSKS 299
KK + H + KG KT +S+ +K+
Sbjct: 771 GKKGGHTATPHPAK--KGGKTPNSEAKAKT 854
Score = 29.3 bits (64), Expect = 2.3
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = +3
Query: 214 KKNFGAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGDWK 256
K + A+N D+DE D +ED+ D P L + EN K + K
Sbjct: 339 KAYYDADNSDEDEFTDSDEDDEDVP------LINTENGKPETK 449
>TC203614 UP|Q94LX2 (Q94LX2) Beta-conglycinin alpha subunit, complete
Length = 1983
Score = 36.6 bits (83), Expect = 0.014
Identities = 19/59 (32%), Positives = 34/59 (57%)
Frame = +3
Query: 218 GAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGDWKQKIRKGIVDTSTTLKKHATK 276
G+E ED+DEDE+++E + FP + + K+ E D +Q+ R+ + L++H K
Sbjct: 444 GSEEEDEDEDEEQDERQFPFPRPPHQKEERKQEEDEDEEQQ-RESEESEDSELRRHKNK 617
>TC230134 weakly similar to UP|NUCL_HUMAN (P19338) Nucleolin (Protein C23),
partial (8%)
Length = 581
Score = 36.6 bits (83), Expect = 0.014
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = +2
Query: 196 EGMPGAPGMKMYSRDDLMKKNFGAENEDDDEDEDEEEDEAD 236
E G G + DD + N G E++DDDED+D+++D+ D
Sbjct: 347 EDFSGDDGGEEADSDDDPEANGGGESDDDDEDDDDDDDDND 469
Score = 34.3 bits (77), Expect = 0.071
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +2
Query: 210 DDLMKKNFGAENEDDDEDEDEEEDEADFPSKLGK 243
DD ++ G E++DD+EDEDE+E+ PSK K
Sbjct: 455 DDDNDEDDGDEDDDDEEDEDEDEETPQPPSKKRK 556
>TC224625 similar to UP|O04692 (O04692) DNA-binding protein, partial (38%)
Length = 682
Score = 36.6 bits (83), Expect = 0.014
Identities = 22/70 (31%), Positives = 39/70 (55%), Gaps = 10/70 (14%)
Frame = +1
Query: 177 PFVAKSSK-EAEMEKLLKSMEGMPGAPG---------MKMYSRDDLMKKNFGAENEDDDE 226
P+ AK++K +AE EKL+++ E + ++ DD + E+EDD+E
Sbjct: 229 PYEAKAAKRKAEYEKLIQAYEKKQASSADDDESDKSKSEVNDEDDASGEEDHQEDEDDEE 408
Query: 227 DEDEEEDEAD 236
+ED+E+D+ D
Sbjct: 409 EEDDEDDD*D 438
>TC219508 homologue to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (62%)
Length = 1291
Score = 35.8 bits (81), Expect = 0.024
Identities = 34/93 (36%), Positives = 45/93 (47%), Gaps = 5/93 (5%)
Frame = +1
Query: 208 SRDDLMKKN-FGAENE-DDDEDEDEEEDEADFPSKLGKILKSKENE---KGDWKQKIRKG 262
S DDL ++ FG+ +E DDD D DEE DE + P+K K + NE K K K
Sbjct: 574 SDDDLAGEDEFGSSDEMDDDSDSDEESDE-ETPAKKVDQGKKRPNESAAKTPVSAKKAKT 750
Query: 263 IVDTSTTLKKHATKVSNHIQRWWKGKKTTSSKK 295
T KK+ + H + KG KT +S K
Sbjct: 751 ATPEKTNGKKNVHVATPHPSK--KGGKTPNSTK 843
>TC227390 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (24%)
Length = 808
Score = 35.4 bits (80), Expect = 0.032
Identities = 42/158 (26%), Positives = 65/158 (40%), Gaps = 15/158 (9%)
Frame = +1
Query: 157 LSKACNTKPPPVPKD-RTP-GEPFVAKSSKEAEMEKLLKSMEGMPGAPGMKMYSRDDLMK 214
L KA N KP ++ + P + VAK+S A+ K+++ P + D
Sbjct: 43 LLKAENGKPETKAEELKVPESKKAVAKASASAKQVKIVE-----PKKDNEEDSDDDSDED 207
Query: 215 KNFGAENE-------------DDDEDEDEEEDEADFPSKLGKILKSKENEKGDWKQKIRK 261
+FG+ +E DD ED+DEE + + LGK ++ K K K
Sbjct: 208 DDFGSSDEEMEDADSDSDDESDDKEDDDEETHPKNKKADLGKKRPNESASKTPVSSKKAK 387
Query: 262 GIVDTSTTLKKHATKVSNHIQRWWKGKKTTSSKKNSKS 299
T KK + H + KG KT SS+ +K+
Sbjct: 388 NATPEKTDGKKGGHTATPHPAK--KGGKTPSSEAKAKT 495
>TC218919 homologue to UP|Q39892 (Q39892) Nucleosome assembly protein 1,
partial (33%)
Length = 729
Score = 34.7 bits (78), Expect = 0.054
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 7/52 (13%)
Frame = +1
Query: 210 DDLMKKNFGAENEDDDEDE-------DEEEDEADFPSKLGKILKSKENEKGD 254
+DL E+ED+DEDE DEEED+ KL + KS + + GD
Sbjct: 181 EDLEDDEDEEEDEDEDEDEEYDEDEDDEEEDDTKTKKKLSAVKKSGKAQAGD 336
Score = 28.9 bits (63), Expect = 3.0
Identities = 22/65 (33%), Positives = 32/65 (48%)
Frame = +1
Query: 218 GAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGDWKQKIRKGIVDTSTTLKKHATKV 277
G E ED ++DEDEEEDE + + + + +++E+ D DT T K A K
Sbjct: 169 GDEFEDLEDDEDEEEDEDEDEDE--EYDEDEDDEEED----------DTKTKKKLSAVKK 312
Query: 278 SNHIQ 282
S Q
Sbjct: 313 SGKAQ 327
>TC204249 UP|Q9SB11 (Q9SB11) Glycinin, complete
Length = 1916
Score = 34.3 bits (77), Expect = 0.071
Identities = 19/62 (30%), Positives = 32/62 (50%), Gaps = 6/62 (9%)
Frame = +1
Query: 218 GAENEDDDEDEDEEEDE------ADFPSKLGKILKSKENEKGDWKQKIRKGIVDTSTTLK 271
G +D D+DEDE+EDE ++ SK + + ++ E + + R G+ + TLK
Sbjct: 1027 GKREQDQDQDEDEDEDEDQPRKSREWRSKKTQPRRPRQEEPRERGCETRNGVEENICTLK 1206
Query: 272 KH 273
H
Sbjct: 1207 LH 1212
Score = 30.0 bits (66), Expect = 1.3
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +1
Query: 220 ENEDDDEDEDEEEDEADFPS 239
E +D+DEDEDE++++ PS
Sbjct: 874 EQQDEDEDEDEDDEDEQIPS 933
Score = 27.7 bits (60), Expect = 6.6
Identities = 12/41 (29%), Positives = 25/41 (60%)
Frame = +1
Query: 214 KKNFGAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGD 254
+ + G +D+DEDEDE++ PS+ GK + ++ ++ +
Sbjct: 946 RPSHGKREQDEDEDEDEDKPRPSRPSQ-GKREQDQDQDEDE 1065
>TC203635 similar to PIR|T46225|T46225 alpha NAC-like protein - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (71%)
Length = 1007
Score = 33.9 bits (76), Expect = 0.092
Identities = 27/106 (25%), Positives = 51/106 (47%), Gaps = 5/106 (4%)
Frame = +1
Query: 163 TKPPPVPKDRTPGEPFVAKSSKEAEMEKLLKSMEG-----MPGAPGMKMYSRDDLMKKNF 217
+ P P K +PG V +S E + E+ L S++ +P +DL K+
Sbjct: 28 SNPLPTHKAMSPGP--VVDASPEVDAEQQLPSVDDATQKKIPQPQEDDAPLVEDL--KDD 195
Query: 218 GAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGDWKQKIRKGI 263
+++D+D+D+D+E+D+ D + K +EK K ++ G+
Sbjct: 196 DKDDDDEDDDDDDEDDKEDDAQGGAEGSKQSRSEKKSRKAMLKLGL 333
>TC215203 similar to UP|Q70Z19 (Q70Z19) Nucleosome assembly protein 1-like
protein 1, partial (31%)
Length = 729
Score = 33.9 bits (76), Expect = 0.092
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +2
Query: 221 NEDDDEDEDEEEDEADFPSKLGKILKSKENEKG 253
++DDDEDEDE+ED D K GK + + G
Sbjct: 266 DDDDDEDEDEDEDGEDEEEKKGKSKSKSKLQSG 364
>TC223409 similar to UP|Q6T4P5 (Q6T4P5) Plasticity related gene 2a, partial
(3%)
Length = 439
Score = 33.9 bits (76), Expect = 0.092
Identities = 18/36 (50%), Positives = 23/36 (63%), Gaps = 5/36 (13%)
Frame = +1
Query: 211 DLMKKNFG-----AENEDDDEDEDEEEDEADFPSKL 241
DL K+F E+E++DE+EDEEEDEA S L
Sbjct: 10 DLKDKSFRFVGEEEEDEEEDEEEDEEEDEAPLSSSL 117
>TC204254 UP|GLC4_SOYBN (P02858) Glycinin G4 precursor [Contains: Glycinin A5
subunit; Glycinin A4 subunit; Glycinin B3 subunit],
partial (17%)
Length = 421
Score = 33.9 bits (76), Expect = 0.092
Identities = 23/94 (24%), Positives = 46/94 (48%), Gaps = 7/94 (7%)
Frame = +1
Query: 187 EMEKLLKSMEGMPGAPGMKMYSRDDLMKKNFGA-------ENEDDDEDEDEEEDEADFPS 239
++ +LL S + +P + + D M+ +F + +ED+DEDED+ ++ S
Sbjct: 16 KLAQLLHSQGLLLCSPELGL*EIYDWMRSSFSSCCYLLHYMDEDEDEDEDQPRKSREWRS 195
Query: 240 KLGKILKSKENEKGDWKQKIRKGIVDTSTTLKKH 273
K + + ++ E + + R G+ + TLK H
Sbjct: 196 KKTQPRRPRQEEPRERGCETRNGVEENICTLKLH 297
>BF597314 similar to PIR|S20007|S20 beta-conglycinin alpha chain precursor -
soybean, partial (30%)
Length = 607
Score = 33.9 bits (76), Expect = 0.092
Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 4/85 (4%)
Frame = +2
Query: 158 SKACNTKPPPVPKDRTPGEPFVAKSSKEAEMEKLLKSMEGMP--GAPGMKMYSRDDLMKK 215
S+ +PPP +R P +P K E + + + P + D ++
Sbjct: 209 SEIARPRPPPQHPEREPHQPG-EKDEDEDDAPRSIPFPRPQPRQDEEHEQREEHDWPHEE 385
Query: 216 NFGAEN--EDDDEDEDEEEDEADFP 238
+N EDDDEDED+E+DE +P
Sbjct: 386 ETRGDNVCEDDDEDEDDEQDELQYP 460
>TC215201 similar to UP|Q70Z19 (Q70Z19) Nucleosome assembly protein 1-like
protein 1, partial (55%)
Length = 904
Score = 33.5 bits (75), Expect = 0.12
Identities = 14/23 (60%), Positives = 18/23 (77%)
Frame = +3
Query: 221 NEDDDEDEDEEEDEADFPSKLGK 243
+ED+DEDEDEEE+E + K GK
Sbjct: 537 DEDEDEDEDEEEEEEEEEEKKGK 605
Score = 30.8 bits (68), Expect = 0.78
Identities = 15/49 (30%), Positives = 28/49 (56%)
Frame = +3
Query: 210 DDLMKKNFGAENEDDDEDEDEEEDEADFPSKLGKILKSKENEKGDWKQK 258
+D+ + ++ E D+DEDEDE+E+E + + +E +KG + K
Sbjct: 498 EDIDEVDYDDEEGDEDEDEDEDEEEEE---------EEEEEKKGKSRSK 617
>TC224564 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (82%)
Length = 833
Score = 33.5 bits (75), Expect = 0.12
Identities = 22/70 (31%), Positives = 38/70 (53%), Gaps = 10/70 (14%)
Frame = +1
Query: 177 PFVAKSSK-EAEMEKLLKSMEGMPGAPG---------MKMYSRDDLMKKNFGAENEDDDE 226
P+ AK++K +AE EKL+K+ + + ++ DD + E EDD+E
Sbjct: 388 PYEAKAAKRKAEYEKLIKAYDKKQASSADDEESDKSKSEVNDEDDASGEE---EEEDDEE 558
Query: 227 DEDEEEDEAD 236
+ED+E+D+ D
Sbjct: 559 EEDDEDDD*D 588
>TC221531 similar to UP|Q84N48 (Q84N48) CRS2-associated factor 2, partial
(5%)
Length = 922
Score = 33.1 bits (74), Expect = 0.16
Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 12/72 (16%)
Frame = +1
Query: 177 PFVAKSSKEAEMEKLLKSMEGMPGAPGMKM----------YSRDDLMKKNFGAENEDDDE 226
P + SSKE + E + E + G+ YS D L + E ED+DE
Sbjct: 496 PAFSLSSKENDAEGSSANFENSYSSDGLHTEEDYAKFEDSYSSDALQTEEEEEEEEDEDE 675
Query: 227 DEDEE--EDEAD 236
DEDE+ +DE D
Sbjct: 676 DEDEDYYDDEDD 711
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.311 0.129 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,949,160
Number of Sequences: 63676
Number of extensions: 193141
Number of successful extensions: 2647
Number of sequences better than 10.0: 174
Number of HSP's better than 10.0 without gapping: 1522
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2007
length of query: 301
length of database: 12,639,632
effective HSP length: 97
effective length of query: 204
effective length of database: 6,463,060
effective search space: 1318464240
effective search space used: 1318464240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148360.3