BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148347.5 - phase: 0
(118 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC207303 weakly similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like prot... 95 5e-21
TC224824 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, pa... 85 4e-18
CD408043 weakly similar to PIR|T07822|T0782 cystein proteinase i... 82 3e-17
TC225002 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, pa... 82 4e-17
BI788429 similar to GP|11596186|gb| cystatin-like protein {Citru... 70 2e-13
TC221610 66 2e-12
BE330223 52 3e-08
TC226074 cysteine proteinase inhibitor 43 2e-05
TC225963 homologue to UP|CYTI_VIGUN (Q06445) Cysteine proteinase... 41 7e-05
TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inh... 35 0.007
TC205866 UP|O04720 (O04720) Cysteine proteinase inhibitor, complete 34 0.011
TC231099 similar to PIR|E86286|E86286 F9L1.18 protein - Arabidop... 27 1.0
TC210289 similar to UP|Q9FUK9 (Q9FUK9) MYB-related transcription... 27 1.4
TC210340 similar to GB|AAM47357.1|21360483|AY113049 AT5g64160/MH... 27 1.8
BU544869 26 3.0
BI424367 similar to SP|P50694|TLP_P Thaumatin-like protein precu... 25 5.2
TC203513 similar to UP|Q8L5P7 (Q8L5P7) LHY protein, partial (42%) 25 5.2
TC204396 similar to GB|AAC36740.1|3643249|AF090143 thaumatin-lik... 25 5.2
TC213728 weakly similar to UP|Q9LZR8 (Q9LZR8) S-receptor kinase-... 25 5.2
TC204571 homologue to UP|Q9SPF9 (Q9SPF9) Ubiquitin-conjugating e... 25 5.2
>TC207303 weakly similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial
(47%)
Length = 479
Score = 94.7 bits (234), Expect = 5e-21
Identities = 61/116 (52%), Positives = 80/116 (68%), Gaps = 3/116 (2%)
Frame = +2
Query: 6 MVLVLVVLLAFTAT-KQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLW 64
+VLV VVLLA + IP G +PI NINDP V ++ANFAV EY+ ++ EKL+L
Sbjct: 62 VVLVFVVLLACAVGWDEGIP-GGWNPIKNINDPHVTEIANFAVTEYD---KQSGEKLKLV 229
Query: 65 KVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLE--WSLQHLRNLTSFKLIQA 118
KVIKG+ Q+VA G+NYRL+L+A+ SN Y+AIV E W+ +H RNLTSF + A
Sbjct: 230 KVIKGDLQVVA-GLNYRLSLTAS---DSNNYQAIVYEKAWAREHYRNLTSFTPLHA 385
>TC224824 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial (42%)
Length = 756
Score = 85.1 bits (209), Expect = 4e-18
Identities = 50/114 (43%), Positives = 73/114 (63%)
Frame = +3
Query: 1 MRLESMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEK 60
MR + ++LV +VL+++ A ++ +G SPI ++ND V ++AN+A+ EY+ R K
Sbjct: 123 MRHQCLLLVSLVLVSYAARSESA-LGGWSPIKDVNDSHVAEIANYALSEYDKRSGA---K 290
Query: 61 LRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
L L KV+KGE+Q+V+ G NYRL L A + +YEAIV E H NLTSFK
Sbjct: 291 LTLVKVVKGETQVVS-GTNYRLVLKAKDGSATASYEAIVWEKPWLHFMNLTSFK 449
>CD408043 weakly similar to PIR|T07822|T0782 cystein proteinase inhibitor -
cucumber, partial (53%)
Length = 606
Score = 82.4 bits (202), Expect = 3e-17
Identities = 48/92 (52%), Positives = 66/92 (71%), Gaps = 2/92 (2%)
Frame = -1
Query: 29 SPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATK 88
+PI NINDP V ++ANFAV EY+ + EKL+L KVIKG+ Q+V+ G+NYRL+L+A+
Sbjct: 474 NPIKNINDPHVTEIANFAVTEYD---KLSGEKLKLVKVIKGDLQVVS-GLNYRLSLTAS- 310
Query: 89 VYTSNTYEAIVLE--WSLQHLRNLTSFKLIQA 118
S+ Y+AIV E W+ +H RNLTSF + A
Sbjct: 309 --DSDNYQAIVYEKAWAREHYRNLTSFTPLHA 220
>TC225002 similar to UP|Q9FQ13 (Q9FQ13) Cystatin-like protein, partial (42%)
Length = 1205
Score = 82.0 bits (201), Expect = 4e-17
Identities = 48/109 (44%), Positives = 70/109 (64%)
Frame = +2
Query: 6 MVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWK 65
++LV +VL+++ A ++ +G SPI ++ND V ++AN+A+ EY+ R KL L K
Sbjct: 290 LLLVSLVLVSYAARSESA-LGGWSPIKDVNDSHVAEIANYALSEYDKRSGA---KLTLVK 457
Query: 66 VIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
V+KGE+Q+V+ G NYRL L A + +YEAIV E H NLTSFK
Sbjct: 458 VVKGETQVVS-GTNYRLVLKAKDGSATASYEAIVWEKPWLHFMNLTSFK 601
>BI788429 similar to GP|11596186|gb| cystatin-like protein {Citrus x
paradisi}, partial (39%)
Length = 365
Score = 69.7 bits (169), Expect = 2e-13
Identities = 43/97 (44%), Positives = 55/97 (56%)
Frame = +1
Query: 18 ATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADG 77
A + +G SPI ++ND V ++AN+A+ EY+ R KL L K KGE+Q V G
Sbjct: 67 AARSESALGGWSPIKDVNDSHVAEIANYALSEYDKRSGA---KLTLVKDGKGETQ-VGSG 234
Query: 78 VNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
NYRL L A + +YEAIV E H NLTSFK
Sbjct: 235 TNYRLVLKAKDGSATASYEAIVWEKPWLHFMNLTSFK 345
>TC221610
Length = 519
Score = 66.2 bits (160), Expect = 2e-12
Identities = 45/123 (36%), Positives = 68/123 (54%), Gaps = 10/123 (8%)
Frame = +3
Query: 1 MRLESMVLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEK 60
++LE ++LVLV++ + A K+ + +G SPI NINDPKVI++AN+A+ ++ R K
Sbjct: 45 IKLECLLLVLVLMTSVVARKEPL-LGRWSPIKNINDPKVIEIANYAIATFDKRF---GTK 212
Query: 61 LRLWKVIKGESQIV---------ADGVNYRLTLSATKVYTS-NTYEAIVLEWSLQHLRNL 110
+L K+I+GE++ V G++Y L A Y Y A V H RNL
Sbjct: 213 KKLEKMIEGETRTVEGLKYKTRIVAGMDYFLIFRANDEYGEFQNYGATVRVIESLHFRNL 392
Query: 111 TSF 113
T F
Sbjct: 393 TGF 401
>BE330223
Length = 268
Score = 52.4 bits (124), Expect = 3e-08
Identities = 37/93 (39%), Positives = 53/93 (56%)
Frame = +2
Query: 7 VLVLVVLLAFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKV 66
+LV +VL+++ A + I +G LSPI ++ND V ++ N+A+ EY R K L K
Sbjct: 2 LLVSLVLVSYAARAE-ITLGGLSPIKDVNDSHVAEIGNYALSEYEER---AGAKRTLGKG 169
Query: 67 IKGESQIVADGVNYRLTLSATKVYTSNTYEAIV 99
IKGE+Q V+ N L L A + YEAIV
Sbjct: 170IKGENQGVS-RTNCPLVLKAKDGSGTARYEAIV 265
>TC226074 cysteine proteinase inhibitor
Length = 828
Score = 42.7 bits (99), Expect = 2e-05
Identities = 31/91 (34%), Positives = 47/91 (51%), Gaps = 1/91 (1%)
Frame = +2
Query: 25 IGNLSPINNI-NDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLT 83
+G ++ ++ N ++ ++A FAV+E N R E +R VI + Q+VA GVNY +T
Sbjct: 20 LGGITDVHGAANSVEINNLARFAVEEQNKRENSVLEFVR---VISAKQQVVA-GVNYYIT 187
Query: 84 LSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
L A N YEA V + + L FK
Sbjct: 188 LEAKDGLIKNEYEAKVWVREWLNSKELLEFK 280
>TC225963 homologue to UP|CYTI_VIGUN (Q06445) Cysteine proteinase inhibitor
(Cystatin), complete
Length = 757
Score = 41.2 bits (95), Expect = 7e-05
Identities = 28/91 (30%), Positives = 45/91 (48%)
Frame = +3
Query: 26 GNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLS 85
GN + N ++ +A FAV+E+N +K L KV+ + Q+V+ G Y +TL
Sbjct: 171 GNRDVAGSQNSLEIDGLARFAVEEHN---KKQNALLEFEKVVSAKQQVVS-GTLYTITLE 338
Query: 86 ATKVYTSNTYEAIVLEWSLQHLRNLTSFKLI 116
A YEA V E + + + + FKL+
Sbjct: 339 AKDGGQKKVYEAKVWEKAWLNFKEVQEFKLV 431
>TC216018 homologue to UP|O04720 (O04720) Cysteine proteinase inhibitor,
complete
Length = 1064
Score = 34.7 bits (78), Expect = 0.007
Identities = 24/73 (32%), Positives = 36/73 (48%)
Frame = +1
Query: 42 VANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLE 101
+A FAV E+N +K L +V++ + Q+VA G + LTL A + YEA V
Sbjct: 241 LARFAVDEHN---KKQNSLLEFARVVRAQEQVVA-GTLHHLTLEAIEAGEKKLYEAKVWV 408
Query: 102 WSLQHLRNLTSFK 114
+ + L FK
Sbjct: 409 KPWLNFKELQEFK 447
>TC205866 UP|O04720 (O04720) Cysteine proteinase inhibitor, complete
Length = 1175
Score = 33.9 bits (76), Expect = 0.011
Identities = 24/73 (32%), Positives = 36/73 (48%)
Frame = +3
Query: 42 VANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLE 101
+A FAV E+N +K L +V++ + Q+VA G + LTL A + YEA V
Sbjct: 243 LARFAVDEHN---KKQNSLLEFSRVVRTQEQVVA-GTLHHLTLEAIEAGEKKLYEAKVWV 410
Query: 102 WSLQHLRNLTSFK 114
+ + L FK
Sbjct: 411 KPWLNFKELQEFK 449
>TC231099 similar to PIR|E86286|E86286 F9L1.18 protein - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (34%)
Length = 669
Score = 27.3 bits (59), Expect = 1.0
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +1
Query: 53 RRRKPEEKLRLWKVIKGE 70
RRR+P EKLR W ++G+
Sbjct: 508 RRRRPSEKLRGWNRVEGQ 561
>TC210289 similar to UP|Q9FUK9 (Q9FUK9) MYB-related transcription factor
PHAN1, partial (68%)
Length = 1009
Score = 26.9 bits (58), Expect = 1.4
Identities = 17/60 (28%), Positives = 27/60 (44%), Gaps = 2/60 (3%)
Frame = +2
Query: 51 NNRRRKPEEKLRLWKVIKGESQIVADGVN--YRLTLSATKVYTSNTYEAIVLEWSLQHLR 108
N RR K EE K ++ E + + YR L+A + N + + +W +HLR
Sbjct: 620 NRRREKIEEFEAKIKALQEEEKAALGRIEAEYREQLAALRRDAENKEQKLAEQWDAKHLR 799
>TC210340 similar to GB|AAM47357.1|21360483|AY113049 AT5g64160/MHJ24_14
{Arabidopsis thaliana;} , partial (28%)
Length = 595
Score = 26.6 bits (57), Expect = 1.8
Identities = 16/58 (27%), Positives = 27/58 (45%)
Frame = +2
Query: 35 NDPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQIVADGVNYRLTLSATKVYTS 92
N+P D K N RRRK ++ L V++ + Q+V R ++ ++ TS
Sbjct: 299 NNPSSRDADEQQGKHTNRRRRKDKKDLPTGAVVEAKPQLVGIHERVRSDINGSQAPTS 472
>BU544869
Length = 588
Score = 25.8 bits (55), Expect = 3.0
Identities = 17/54 (31%), Positives = 26/54 (47%)
Frame = -2
Query: 61 LRLWKVIKGESQIVADGVNYRLTLSATKVYTSNTYEAIVLEWSLQHLRNLTSFK 114
L +V++ + Q+VA G + LTL A + YEA V + + L FK
Sbjct: 275 LEFSRVVRTQEQVVA-GTLHHLTLEAIEAGEKKLYEAKVWVKPWLNFKELQEFK 117
>BI424367 similar to SP|P50694|TLP_P Thaumatin-like protein precursor.
[Cherry] {Prunus avium}, partial (26%)
Length = 421
Score = 25.0 bits (53), Expect = 5.2
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = +3
Query: 98 IVLEWSLQHLRNLTSFKLI 116
I+L W+ QH RN+++++L+
Sbjct: 348 ILLHWT*QHRRNMSTYELL 404
>TC203513 similar to UP|Q8L5P7 (Q8L5P7) LHY protein, partial (42%)
Length = 1163
Score = 25.0 bits (53), Expect = 5.2
Identities = 11/38 (28%), Positives = 20/38 (51%)
Frame = +1
Query: 36 DPKVIDVANFAVKEYNNRRRKPEEKLRLWKVIKGESQI 73
+P++ D A+ E++NRRR WK + E ++
Sbjct: 832 EPEIPDANQLAI-EFSNRRRSVSNLTDSWKEVSEEGRL 942
>TC204396 similar to GB|AAC36740.1|3643249|AF090143 thaumatin-like protein
precursor Mdtl1 {Malus x domestica;} , partial (92%)
Length = 1028
Score = 25.0 bits (53), Expect = 5.2
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = +1
Query: 98 IVLEWSLQHLRNLTSFKLI 116
I+L W+ QH RN+++++L+
Sbjct: 607 ILLHWT*QHRRNMSTYELL 663
>TC213728 weakly similar to UP|Q9LZR8 (Q9LZR8) S-receptor kinase-like
protein, partial (24%)
Length = 659
Score = 25.0 bits (53), Expect = 5.2
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 54 RRKPEEKLRLWKVIKGESQIVAD 76
RRKP EKL LW + + +V D
Sbjct: 344 RRKPREKLPLWSLRTA*NGVVED 276
>TC204571 homologue to UP|Q9SPF9 (Q9SPF9) Ubiquitin-conjugating enzyme UBC2,
complete
Length = 1491
Score = 25.0 bits (53), Expect = 5.2
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = +2
Query: 15 AFTATKQAIPIGNLSPINNINDPKVIDVANFAVKEYNNRRRKPEEKLRLW 64
A T +K + I +L N +DP V D+A+ Y R K E R W
Sbjct: 1043 ALTVSKVLLSICSLLTDPNPDDPLVPDIAHM----YKTDRDKYESTARSW 1180
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.134 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,340,395
Number of Sequences: 63676
Number of extensions: 44857
Number of successful extensions: 279
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 269
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 269
length of query: 118
length of database: 12,639,632
effective HSP length: 94
effective length of query: 24
effective length of database: 6,654,088
effective search space: 159698112
effective search space used: 159698112
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC148347.5