BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.14 - phase: 0
(497 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222199 117 9e-27
TC216808 57 2e-18
BI317622 56 4e-08
BG508280 55 9e-08
BE611176 37 5e-04
BI315644 33 0.29
TC207513 similar to UP|SY51_ARATH (Q9SA23) Syntaxin 51 (AtSYP51)... 30 2.5
TC218692 similar to UP|Q9SH45 (Q9SH45) F2K11.3, partial (47%) 29 4.2
TC232048 similar to UP|ACOC_CUCMA (P49608) Aconitate hydratase, ... 28 9.5
>TC222199
Length = 691
Score = 117 bits (294), Expect = 9e-27
Identities = 66/226 (29%), Positives = 111/226 (48%), Gaps = 16/226 (7%)
Frame = +2
Query: 96 DYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKLTLKGNLPS 155
D+QL+PT+EE+ ++G P+ ++ P+ P + I + + + +
Sbjct: 5 DFQLVPTIEEFEEILGCPLGERKPYLSSGCLPSLSRIPTMVKDSARGLDRVKQTRNGIAG 184
Query: 156 LPTKFLYQQASEFSKTNNVEAFYSILALLIYGLVLFPNIDNYVDIHAIQIFLT-----KN 210
LP K+L +A + + F +LALLI+G+VLFPN+D VD+ AI FL ++
Sbjct: 185 LPRKYLEDKARGIANQGDWVPFMDVLALLIFGVVLFPNVDGLVDLAAIDAFLAYHHSKES 364
Query: 211 PVPTLLADIYHSIHDRTQVGRGAILGCAPLLYKWFTSHLPQ----------THSFQANPE 260
PV +LAD++ + + R + I+ C P L W SHL Q +H
Sbjct: 365 PVVAVLADLFDTFNRRYEKSSARIICCLPALCVWLVSHLFQQDTRRPCPLLSHRSCTEKR 544
Query: 261 NLSWPKRIMSLTPSDITWY-RATCNFTNIIVSCGEYSNVPLLGTQG 305
+ W + + + I+W+ R ++ SCG Y N+PL+GT+G
Sbjct: 545 RIDWDQLLAGIGGRTISWFPRWKEGKEGVLFSCGRYPNIPLVGTRG 682
>TC216808
Length = 1144
Score = 57.0 bits (136), Expect(2) = 2e-18
Identities = 33/79 (41%), Positives = 46/79 (57%), Gaps = 5/79 (6%)
Frame = +3
Query: 176 AFYSILALLIYGLVLFPNIDNYVDIHAIQIFLT-----KNPVPTLLADIYHSIHDRTQVG 230
+F ILALLI+G+VLFPN++ VD+ AI FL ++PV +LA +Y + +
Sbjct: 591 SFIDILALLIFGVVLFPNMEGLVDLAAIDAFLAFHHGKESPVVAILA-MYDTFDRGCEKS 767
Query: 231 RGAILGCAPLLYKWFTSHL 249
I+ CA LY W SHL
Sbjct: 768 SARIVCCALALYVWLVSHL 824
Score = 53.5 bits (127), Expect(2) = 2e-18
Identities = 31/143 (21%), Positives = 63/143 (43%), Gaps = 2/143 (1%)
Frame = +2
Query: 38 RKLDLENLKKLAFEVTSLEN--FRDRHGNLLGVLWTNIEKGCLETLVQFYDPAYHCFTFP 95
+ L++ +L +L + L+ FR +G + + + + +L Q+YD CFTF
Sbjct: 170 KSLEVTSLGELGQLMGQLQR*AFRKAYGKIWDLARVEVSMEPIASLSQYYDQPLRCFTFE 349
Query: 96 DYQLMPTLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKLTLKGNLPS 155
D+QL+PT++E + G + + P+ +P +A + + + + +
Sbjct: 350 DFQLVPTVKELEEIRGCQLGGRKPYLFSGFYPSTTRVAKVVKISAQELNRVKQNRNGVVG 529
Query: 156 LPTKFLYQQASEFSKTNNVEAFY 178
+P K L ++A FY
Sbjct: 530 VPRKCLEERAKTLENQGEWAFFY 598
>BI317622
Length = 370
Score = 55.8 bits (133), Expect = 4e-08
Identities = 34/123 (27%), Positives = 57/123 (45%), Gaps = 16/123 (13%)
Frame = -2
Query: 195 DNYVDIHAIQIFLTKN-----PVPTLLADIYHSIHDRTQVGRGAILGCAPLLYKWFTSHL 249
D VD+ AI FL + P+ +LAD+Y + + R + I+ C P LY W SHL
Sbjct: 369 DGLVDLAAIGAFLAYHDYKEIPIVAMLADLYDTFNRRCEKSSTRIVCCTPALYVWLVSHL 190
Query: 250 ----------PQTHSFQANPENLSWPKRIMSLTPSDITWY-RATCNFTNIIVSCGEYSNV 298
++H +W + + + + + W+ R T +++SCG + NV
Sbjct: 189 FRQEVRHACPLESHCSCTEKGGENWGQLLANKEGASVNWFPRWKEGRTRVLISCGVFLNV 10
Query: 299 PLL 301
PL+
Sbjct: 9 PLM 1
>BG508280
Length = 418
Score = 54.7 bits (130), Expect = 9e-08
Identities = 23/41 (56%), Positives = 31/41 (75%)
Frame = +1
Query: 286 TNIIVSCGEYSNVPLLGTQGGISYNPILAKRQFGCPMEAKP 326
T +++SCG + NVPL+GT+G ISYNP+LA +Q G PM P
Sbjct: 64 TRVLISCGGFPNVPLMGTRGCISYNPVLAIKQLGYPM*GVP 186
>BE611176
Length = 424
Score = 37.0 bits (84), Expect(2) = 5e-04
Identities = 24/88 (27%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Frame = -2
Query: 16 GLILVFVNIIGMERRNTRKFT--FRKLDLENLKKLA--FEVTSLENFRDRHGNLLGVLWT 71
G VN+ G ++ R + + LD+ ++K+L ++ FR + +L +
Sbjct: 405 GFCFTLVNM-GTDQTGKRFYQVKIKGLDITSIKELGRLMGPLQMQAFRKAYEKILDLTIA 229
Query: 72 NIEKGCLETLVQFYDPAYHCFTFPDYQL 99
I + +L Q+YD CFTF D+QL
Sbjct: 228 EISTEAIASLTQYYD*PLRCFTFGDFQL 145
Score = 24.6 bits (52), Expect(2) = 5e-04
Identities = 10/29 (34%), Positives = 18/29 (61%), Gaps = 2/29 (6%)
Frame = -1
Query: 99 LMPTLEEYSHLIGLPVLDKVP--FTGLEP 125
+ PT+EE+ ++G P+ + P F+G P
Sbjct: 148 IRPTVEEFEEILGCPLRGRKPYFFSGFLP 62
>BI315644
Length = 421
Score = 33.1 bits (74), Expect = 0.29
Identities = 14/26 (53%), Positives = 18/26 (68%)
Frame = +3
Query: 303 TQGGISYNPILAKRQFGCPMEAKPDN 328
T+G I+YNP+LA RQ G PM P +
Sbjct: 276 TRGCINYNPVLAIRQLGYPMRGVPSD 353
>TC207513 similar to UP|SY51_ARATH (Q9SA23) Syntaxin 51 (AtSYP51), partial
(97%)
Length = 1110
Score = 30.0 bits (66), Expect = 2.5
Identities = 22/92 (23%), Positives = 43/92 (45%), Gaps = 10/92 (10%)
Frame = +2
Query: 396 LSSTTPAPALPLAPATVEECQEKLARSECVSATWKRKYDEAMLKVETISGKIEQQ----- 450
++S TP L + + + +A S S +W ++Y+EA+ + ISG I +Q
Sbjct: 146 IASITPFLFLTVTAVVASQKRVPMASS---SDSWVKEYNEALKLADDISGMISEQSSFPA 316
Query: 451 -----EHEVHKLRRQIVKKNVQIRAQSTRLSQ 477
+H +RR+I ++ + + LS+
Sbjct: 317 SGPETQHHSSAIRRKITILGTRLDSLQSLLSK 412
>TC218692 similar to UP|Q9SH45 (Q9SH45) F2K11.3, partial (47%)
Length = 644
Score = 29.3 bits (64), Expect = 4.2
Identities = 27/68 (39%), Positives = 33/68 (47%), Gaps = 2/68 (2%)
Frame = -1
Query: 102 TLEEYSHLIGLPVLDKVPFTGLEPFPKAATIANALHLKTSLIKEKL--TLKGNLPSLPTK 159
T YS I L + DKVP GLE A L+ SL++E TL+ NLP L K
Sbjct: 404 TFLTYSSKISLLLFDKVPLGGLESPALAG-------LELSLLEESYASTLRPNLP-LGFK 249
Query: 160 FLYQQASE 167
FL + E
Sbjct: 248 FLEKLIDE 225
>TC232048 similar to UP|ACOC_CUCMA (P49608) Aconitate hydratase, cytoplasmic
(Citrate hydro-lyase) (Aconitase) , partial (28%)
Length = 882
Score = 28.1 bits (61), Expect = 9.5
Identities = 17/62 (27%), Positives = 27/62 (43%), Gaps = 3/62 (4%)
Frame = -2
Query: 278 WYRATCNFTNIIV---SCGEYSNVPLLGTQGGISYNPILAKRQFGCPMEAKPDNIYLQGE 334
W FT + S Y+ + + T G SYN I + CP+E + N+ L+
Sbjct: 701 WLHLQSQFTCFLCNQCSSKHYARI*SICTTGDSSYNNITVPKMCWCPLECELCNLILRFF 522
Query: 335 FY 336
+Y
Sbjct: 521 WY 516
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,806,605
Number of Sequences: 63676
Number of extensions: 367341
Number of successful extensions: 1975
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1969
length of query: 497
length of database: 12,639,632
effective HSP length: 101
effective length of query: 396
effective length of database: 6,208,356
effective search space: 2458508976
effective search space used: 2458508976
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148289.14