BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148289.1 + phase: 0
(340 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC222695 weakly similar to UP|Q6ZIB5 (Q6ZIB5) PIN1-like auxin tr... 304 e-104
TC233634 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport... 175 2e-44
TC204675 homologue to UP|Q8GV76 (Q8GV76) Auxin efflux carrier pr... 162 2e-40
TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier pr... 161 5e-40
TC220216 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport... 160 1e-39
BG839278 homologue to GP|25986771|gb auxin efflux carrier protei... 152 2e-37
TC211544 weakly similar to UP|Q84YG8 (Q84YG8) Auxin efflux carri... 145 3e-35
TC234581 homologue to UP|Q84YG8 (Q84YG8) Auxin efflux carrier pr... 130 9e-31
AW596376 weakly similar to GP|10441744|gb| PIN1-like auxin trans... 115 3e-26
TC227966 similar to UP|Q8H1L0 (Q8H1L0) PIN-like protein, partial... 114 8e-26
TC220979 similar to UP|Q7X9P6 (Q7X9P6) PIN1-like protein, partia... 80 2e-15
BE191508 similar to GP|10441744|gb| PIN1-like auxin transport pr... 71 6e-13
BI974039 similar to GP|25956262|dbj PIN1-like auxin transport pr... 70 1e-12
TC204038 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydrat... 46 3e-05
BG725422 33 0.14
TC215984 similar to UP|Q9FMP3 (Q9FMP3) Dihydropyrimidinase (Dihy... 32 0.54
TC222538 31 0.92
BE660724 homologue to SP|Q9SBW3|P2A4_ Serine/threonine protein p... 29 3.5
BE058570 29 3.5
TC215821 similar to UP|Q9LW00 (Q9LW00) Arabidopsis thaliana geno... 29 3.5
>TC222695 weakly similar to UP|Q6ZIB5 (Q6ZIB5) PIN1-like auxin transport
protein-like, partial (41%)
Length = 1059
Score = 304 bits (779), Expect(2) = e-104
Identities = 162/220 (73%), Positives = 183/220 (82%), Gaps = 7/220 (3%)
Frame = +1
Query: 128 MGVDLVVQASVVQAIIWLTLLLFVLEFRRTGIEGTITT-LKPKASSISNVTCEGEESKDV 186
+GVDLVVQ+SV+QA IWL LLLFV+EF RTGIEGT TT +KP++ ++ N EG KDV
Sbjct: 208 LGVDLVVQSSVLQATIWLPLLLFVMEFWRTGIEGTTTTTVKPRSKTMINKN-EGGGGKDV 384
Query: 187 EANNIVEY------TSSRLPFLQLMKRVWLKLIANPNSYGCVIGISWAFISNRWNLELPS 240
EA ++ E +SRLPF ++MK VW KL NPNSYGCVIGISWAFISNRWNLE+PS
Sbjct: 385 EAVDVKEELMLEESVTSRLPFCKVMKLVWRKLATNPNSYGCVIGISWAFISNRWNLEMPS 564
Query: 241 MVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAFI 300
M+EGSI IMSKAGTGTAMFSMGIFMALQEK+I+CGPS+T+ GLVLKFIAGPAA AI A
Sbjct: 565 MLEGSIQIMSKAGTGTAMFSMGIFMALQEKLIACGPSMTLIGLVLKFIAGPAATAIGAIA 744
Query: 301 VGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLSTA 340
VGLRGDVLR+ IIQAAVPQSITSFIFAKEY LH EVLSTA
Sbjct: 745 VGLRGDVLRVVIIQAAVPQSITSFIFAKEYGLHPEVLSTA 864
Score = 92.8 bits (229), Expect(2) = e-104
Identities = 41/59 (69%), Positives = 50/59 (84%)
Frame = +2
Query: 67 MNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPMVKPMY 125
MNF FI ADT+SK II+ V+ALWAKC+ K ++ WSI SFSLC LTN++VVG+PMVKPMY
Sbjct: 26 MNFSFIAADTISKFIIMVVLALWAKCTPKCTFCWSIISFSLCNLTNAVVVGVPMVKPMY 202
>TC233634 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport protein,
partial (41%)
Length = 817
Score = 175 bits (444), Expect = 2e-44
Identities = 83/155 (53%), Positives = 112/155 (71%)
Frame = +2
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI D+Y V+ AVVPLY A+IL YGSV+WWKIFT +QC IN+ V F +PL +F F +
Sbjct: 89 MITLWDLYHVLTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIS 268
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P+ MN+ FI AD+L K I++AV+ +W++ SS+ S WSIT FSL TL N+LV+GIP+
Sbjct: 269 TNNPYAMNYKFIAADSLQKAIVLAVLLVWSRTSSRGSLEWSITLFSLSTLPNTLVMGIPL 448
Query: 121 VKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+K MYG L+VQ V+Q IIW TL+LF+ E+R
Sbjct: 449 LKGMYGDASGTLMVQIVVLQCIIWYTLMLFLFEYR 553
>TC204675 homologue to UP|Q8GV76 (Q8GV76) Auxin efflux carrier protein,
partial (40%)
Length = 2164
Score = 162 bits (411), Expect = 2e-40
Identities = 75/161 (46%), Positives = 111/161 (68%), Gaps = 5/161 (3%)
Frame = +1
Query: 185 DVEANNIVEYTSSRLPFLQLMKR-----VWLKLIANPNSYGCVIGISWAFISNRWNLELP 239
++ + V +P +M R VW KLI NPN+Y +IG+ W+ ++ RW++++P
Sbjct: 496 ELHPKSAVAVAGKHMPPASVMTRLILIMVWRKLIRNPNTYSSLIGVIWSLVAFRWHVQMP 675
Query: 240 SMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAF 299
++E SI I+S AG G AMFS+G+FMALQ K+I+CG S+ F + ++F+ GPA MA ++
Sbjct: 676 KIIEKSISILSDAGLGMAMFSLGLFMALQPKIIACGNSVATFAMAVRFLTGPAVMAAASI 855
Query: 300 IVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLSTA 340
VGLRG +LR+AI+QAA+PQ I F+FAKEY +H +LSTA
Sbjct: 856 AVGLRGTLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTA 978
>TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier protein PIN1,
partial (56%)
Length = 1532
Score = 161 bits (407), Expect = 5e-40
Identities = 75/164 (45%), Positives = 112/164 (67%), Gaps = 5/164 (3%)
Frame = +2
Query: 182 ESKDVEANNIVEYTSSRLPFLQLMKR-----VWLKLIANPNSYGCVIGISWAFISNRWNL 236
E +E + + +P +M R VW KLI NPN+Y +IG++W+ +S +WN+
Sbjct: 599 EMNQLEGEKVGDGKPKTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFKWNV 778
Query: 237 ELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAI 296
E+P+++ SI I+S AG G AMFS+G+FMALQ +VI+CG S F + ++F+ GPA MA
Sbjct: 779 EMPAIIAKSISILSDAGLGMAMFSLGLFMALQPRVIACGNSTAAFAMAVRFLTGPAVMAA 958
Query: 297 SAFIVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLSTA 340
++ VGL+G +L +AI+QAA+PQ I F+FAKEY +H ++LSTA
Sbjct: 959 ASIAVGLKGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILSTA 1090
>TC220216 similar to UP|Q8LKH0 (Q8LKH0) PIN1-like auxin transport protein,
partial (36%)
Length = 970
Score = 160 bits (404), Expect = 1e-39
Identities = 71/132 (53%), Positives = 102/132 (76%)
Frame = +2
Query: 208 VWLKLIANPNSYGCVIGISWAFISNRWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMAL 267
VW KLI NPN+Y + G++W+ IS +WN+ +P++V SI I+S AG G AMFS+G+FMAL
Sbjct: 167 VWRKLIRNPNTYSSLFGLTWSLISFKWNVVMPAIVAKSISILSDAGLGMAMFSLGLFMAL 346
Query: 268 QEKVISCGPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLRIAIIQAAVPQSITSFIFA 327
Q K+I+CG S+ F + ++F+ GPA MA+++ +VGLRG +L IAI+QAA+PQ I F+FA
Sbjct: 347 QPKIIACGNSVASFAMAVRFLTGPAVMAVASIVVGLRGVLLHIAIVQAALPQGIVPFVFA 526
Query: 328 KEYELHAEVLST 339
KEY +H ++LST
Sbjct: 527 KEYNVHPDILST 562
>BG839278 homologue to GP|25986771|gb auxin efflux carrier protein {Medicago
truncatula}, partial (19%)
Length = 744
Score = 152 bits (384), Expect = 2e-37
Identities = 74/143 (51%), Positives = 96/143 (66%)
Frame = +2
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI W D Y V+ AV+PLY A+IL YGSVRWWKIF+ +QC IN+ V F +PL +F F +
Sbjct: 314 MITWGDFYTVMSAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 493
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P+ MNF FI ADTL K+I++ +A+W S+ S W IT FSL TL N+LV+GIP+
Sbjct: 494 LNNPYAMNFRFIAADTLQKIIMLFALAIWTNFSANGSLEWMITIFSLSTLPNTLVMGIPL 673
Query: 121 VKPMYGPMGVDLVVQASVVQAII 143
+ MYG L+VQ V Q II
Sbjct: 674 LIAMYGEYSGLLMVQVVVXQCII 742
>TC211544 weakly similar to UP|Q84YG8 (Q84YG8) Auxin efflux carrier protein,
partial (26%)
Length = 573
Score = 145 bits (365), Expect = 3e-35
Identities = 68/155 (43%), Positives = 97/155 (61%)
Frame = +3
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTA 60
MI +Y ++ A+VPLY +IL Y SV WWKIFT EQC IN+LV +++P F F A
Sbjct: 78 MIDGVAIYNIVAALVPLYVPMILAYASVLWWKIFTPEQCSGINRLVALYSVPFLNFNFLA 257
Query: 61 HIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSFSLCTLTNSLVVGIPM 120
+P+ MN FI ADTL K+II+ + LW+ + + +IT FS T N+L+VG+P
Sbjct: 258 FNNPYAMNLRFIAADTLQKVIILGALFLWSAFTKCGNLD*TITLFSFSTFPNTLIVGVPF 437
Query: 121 VKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
+ MYG L+ Q V+Q ++W TL+LF+ E+R
Sbjct: 438 LTAMYGDSSGSLMSQIFVMQGVVWFTLMLFLYEYR 542
>TC234581 homologue to UP|Q84YG8 (Q84YG8) Auxin efflux carrier protein,
partial (21%)
Length = 595
Score = 130 bits (327), Expect = 9e-31
Identities = 59/108 (54%), Positives = 86/108 (79%)
Frame = +2
Query: 233 RWNLELPSMVEGSILIMSKAGTGTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPA 292
R ++E+P++V+GSI I+S AG G AMFS+G+FMALQ K+I+CG S+ F + ++F+ GPA
Sbjct: 2 RGHIEMPTIVKGSISILSDAGLGMAMFSLGLFMALQPKIIACGKSVAAFSMAVRFLTGPA 181
Query: 293 AMAISAFIVGLRGDVLRIAIIQAAVPQSITSFIFAKEYELHAEVLSTA 340
+A ++ +GLRG +L +AI+QAA+PQ I F+FAKEY LHA++LSTA
Sbjct: 182 VIAATSIGIGLRGVLLHVAIVQAALPQGIVPFVFAKEYNLHADILSTA 325
>AW596376 weakly similar to GP|10441744|gb| PIN1-like auxin transport protein
{Populus tremula x Populus tremuloides}, partial (16%)
Length = 416
Score = 115 bits (288), Expect = 3e-26
Identities = 53/119 (44%), Positives = 81/119 (67%)
Frame = +1
Query: 195 TSSRLPFLQLMKRVWLKLIANPNSYGCVIGISWAFISNRWNLELPSMVEGSILIMSKAGT 254
T S+ L ++ +V KLI NPN+Y IG+ WA I RW +++P +V SI I++ G
Sbjct: 58 TQSKTRMLLILMKVGNKLIINPNTYATFIGLIWASIHFRWGVDMPDVVNQSIEILASGGL 237
Query: 255 GTAMFSMGIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLRIAII 313
G A FS+G+FMA ++I CGP +T+ + LKF+AGPA MA+++ ++GLR +L++AII
Sbjct: 238 GMATFSLGLFMASSNRIIVCGPRMTLVAMGLKFLAGPAIMAVASIVIGLRDRMLKVAII 414
>TC227966 similar to UP|Q8H1L0 (Q8H1L0) PIN-like protein, partial (52%)
Length = 992
Score = 114 bits (284), Expect = 8e-26
Identities = 53/110 (48%), Positives = 76/110 (68%)
Frame = +1
Query: 46 VCYFTLPLFAFEFTAHIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSWSITSF 105
V F +PL +F F A +P++MN F+ ADTL K+II+ ++A+W+ + + W+IT F
Sbjct: 7 VALFAVPLLSFHFIASNNPYEMNLRFLAADTLQKIIILVLLAVWSNITKRGCLEWAITLF 186
Query: 106 SLCTLTNSLVVGIPMVKPMYGPMGVDLVVQASVVQAIIWLTLLLFVLEFR 155
SL TL N+LV+GIP++K MYG L+VQ V+Q IIW TL+LF+ EFR
Sbjct: 187 SLSTLPNTLVMGIPLLKGMYGDFSGSLMVQIVVLQCIIWYTLMLFLFEFR 336
>TC220979 similar to UP|Q7X9P6 (Q7X9P6) PIN1-like protein, partial (25%)
Length = 660
Score = 79.7 bits (195), Expect = 2e-15
Identities = 38/78 (48%), Positives = 53/78 (67%), Gaps = 5/78 (6%)
Frame = +2
Query: 196 SSRLPFLQLMKR-----VWLKLIANPNSYGCVIGISWAFISNRWNLELPSMVEGSILIMS 250
S +P +M R VW KLI NPN+Y +IG+ W+ I+ RW++ +P ++E SI I+S
Sbjct: 422 SKHMPPASVMTRLILIMVWRKLIRNPNTYSSLIGVVWSLIAFRWHVHMPKIIEKSISILS 601
Query: 251 KAGTGTAMFSMGIFMALQ 268
AG G AMFS+G+FMALQ
Sbjct: 602 DAGLGMAMFSLGLFMALQ 655
>BE191508 similar to GP|10441744|gb| PIN1-like auxin transport protein
{Populus tremula x Populus tremuloides}, partial (9%)
Length = 478
Score = 71.2 bits (173), Expect = 6e-13
Identities = 30/57 (52%), Positives = 39/57 (67%)
Frame = -3
Query: 1 MIGWEDVYKVIVAVVPLYFALILGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFE 57
MI DVY V+ A VPLY + L Y SV+WWK+FT EQC INK V F++P+ +F+
Sbjct: 173 MISLADVYHVVTATVPLYVTMFLAYISVKWWKLFTPEQCSGINKFVANFSVPILSFQ 3
>BI974039 similar to GP|25956262|dbj PIN1-like auxin transport protein
{Cucumis sativus}, partial (7%)
Length = 421
Score = 70.1 bits (170), Expect = 1e-12
Identities = 31/60 (51%), Positives = 45/60 (74%)
Frame = +1
Query: 262 GIFMALQEKVISCGPSLTVFGLVLKFIAGPAAMAISAFIVGLRGDVLRIAIIQAAVPQSI 321
G+FMALQ +VI+CG S F + ++F+ GPA MA ++ VGL+G +L +AI+QAA+PQ I
Sbjct: 241 GLFMALQPRVIACGNSTAAFAMAVRFLTGPAVMAAASVAVGLKGVLLHVAIVQAALPQGI 420
>TC204038 similar to UP|Q8VZC0 (Q8VZC0) DTDP-glucose 4-6-dehydratase-like
protein, partial (37%)
Length = 1102
Score = 45.8 bits (107), Expect = 3e-05
Identities = 21/33 (63%), Positives = 26/33 (78%)
Frame = +3
Query: 233 RWNLELPSMVEGSILIMSKAGTGTAMFSMGIFM 265
RWNLE+ SM+EGSI IMSKA TGT MF + + +
Sbjct: 732 RWNLEMSSMLEGSIQIMSKAETGTTMFLVWVLI 830
>BG725422
Length = 408
Score = 33.5 bits (75), Expect = 0.14
Identities = 18/29 (62%), Positives = 20/29 (68%)
Frame = +1
Query: 240 SMVEGSILIMSKAGTGTAMFSMGIFMALQ 268
SM+EGSI IMSKA TGT MF + F Q
Sbjct: 4 SMLEGSIQIMSKAETGTTMFLVWEFGVAQ 90
>TC215984 similar to UP|Q9FMP3 (Q9FMP3) Dihydropyrimidinase
(Dihydropyrimidine amidohydrolase) , partial (90%)
Length = 1922
Score = 31.6 bits (70), Expect = 0.54
Identities = 23/100 (23%), Positives = 41/100 (41%), Gaps = 6/100 (6%)
Frame = -3
Query: 52 PLFAFEFTAHIDPFKMNFLFIGADTLSKLIIVAVIALWAKCSSKVSYSW------SITSF 105
PL+A + + PF + I DT+S + VIA+W S + +++
Sbjct: 603 PLYAMKNLKELMPFSLTMSSISLDTVSSHL---VIAIWKP*SMQDFFAFFS*ASKPAVKL 433
Query: 106 SLCTLTNSLVVGIPMVKPMYGPMGVDLVVQASVVQAIIWL 145
LC +T S+ + +P P+ V S+ W+
Sbjct: 432 PLCGITKSMCIVVPPANAAA*PLKKSSTVSVSINSNSRWV 313
>TC222538
Length = 601
Score = 30.8 bits (68), Expect = 0.92
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 37 EQCDAINKLVCYFTLPLFAFEFTAHIDPFKMNFLF 71
EQC +N+++CY LF + D +K N+ F
Sbjct: 416 EQCTKLNQVLCYKKKELFLLNLSEPCDKYKANYSF 520
>BE660724 homologue to SP|Q9SBW3|P2A4_ Serine/threonine protein phosphatase
PP2A-4 catalytic subunit (EC 3.1.3.16). [Rice] {Oryza
sativa}, partial (19%)
Length = 452
Score = 28.9 bits (63), Expect = 3.5
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 7 VYKVIVAVVPLYFALILGYGSVRWWKIFT 35
+ +V+ + + YF I YGS WKIFT
Sbjct: 277 ILEVVQSYIGEYFYFICRYGSANVWKIFT 363
>BE058570
Length = 350
Score = 28.9 bits (63), Expect = 3.5
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = -1
Query: 158 GIEGTITTLKPKASSISNVTCEGEESKDVE 187
G+ G LK ASSI N C G+E +D+E
Sbjct: 290 GLMGLDIVLK*XASSIINSLCGGDEEEDIE 201
>TC215821 similar to UP|Q9LW00 (Q9LW00) Arabidopsis thaliana genomic DNA,
chromosome 3, P1 clone: MSJ11, partial (40%)
Length = 1145
Score = 28.9 bits (63), Expect = 3.5
Identities = 20/48 (41%), Positives = 25/48 (51%)
Frame = -2
Query: 23 LGYGSVRWWKIFTREQCDAINKLVCYFTLPLFAFEFTAHIDPFKMNFL 70
LG+ SVR + F C KL+C+FT L F+ HI PF M L
Sbjct: 769 LGFFSVR--RFFGNFLCWFNFKLLCFFTFRL----FSNHIFPFHMTIL 644
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,923,746
Number of Sequences: 63676
Number of extensions: 223841
Number of successful extensions: 1683
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1673
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1682
length of query: 340
length of database: 12,639,632
effective HSP length: 98
effective length of query: 242
effective length of database: 6,399,384
effective search space: 1548650928
effective search space used: 1548650928
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 59 (27.3 bits)
Medicago: description of AC148289.1