BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148236.5 - phase: 0 /pseudo
(478 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC207975 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 236 2e-62
TC208177 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 213 2e-55
TC215981 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, part... 127 1e-29
AI973706 92 7e-19
TC215982 weakly similar to UP|Q6NKW8 (Q6NKW8) At2g22620, partial... 86 3e-17
BI700921 similar to GP|16323222|gb| AT4g24430/T22A6_260 {Arabido... 59 6e-09
BQ630617 57 1e-08
BE331732 similar to PIR|S25484|S254 ribulose-bisphosphate carbox... 37 0.015
TC213795 32 0.48
TC217911 similar to UP|O23605 (O23605) Sperm protein homolog, pa... 29 5.3
TC232995 28 9.0
>TC207975 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (49%)
Length = 1009
Score = 236 bits (601), Expect = 2e-62
Identities = 100/136 (73%), Positives = 120/136 (87%)
Frame = +2
Query: 332 FWTITDDKGYFSIINVLPGDYNLYSWVNGFIGDYQCNNIINITSGSEINVGELVYEPPRH 391
FW DD GYFS+ N+ GDYN+Y+WV GFIGDY+ + +INIT G +I++G+LVYEPPR
Sbjct: 2 FWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDYKYDVVINITEGCDIDLGDLVYEPPRD 181
Query: 392 GPTLWEIGIPDRSAAEFYVPDPNPLYVNRLYVNHSDRFRQYGLWERYADLYPNEDLVYTV 451
GPTLWEIGIPDRSAAEFYVPDPNP Y+N+LYVNH D+FRQYGLWERYA+LYP++DL+YT+
Sbjct: 182 GPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTI 361
Query: 452 GISDYKKDWFFAQVTR 467
G+SDY KDWFFAQVTR
Sbjct: 362 GVSDYTKDWFFAQVTR 409
>TC208177 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (59%)
Length = 1304
Score = 213 bits (542), Expect = 2e-55
Identities = 104/166 (62%), Positives = 124/166 (74%)
Frame = +1
Query: 163 SNQVHGWISIDSSSDSTGCWLITPSYEFRSAGPLKQYLGSHLGPTMLSVFHSTHYSGADL 222
+N+VHG + SS G W ITPS EFRS GPLKQ L SH+GPT L++F S+HY+G DL
Sbjct: 10 NNRVHGGGYL--SSPPVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLAMFLSSHYAGQDL 183
Query: 223 IMKFGENEPWKKVFGPIFIYLNSNSDGFSPIKLWEDAKQQMVNEVESWPYTFPASEDFLS 282
+ KF E WKKVFGP++IYLNS + G +P+ LWEDAK QM+NEV+SWPY+FPASEDFL
Sbjct: 184 VPKFRGGESWKKVFGPVYIYLNSGAVGDNPLWLWEDAKIQMMNEVQSWPYSFPASEDFLK 363
Query: 283 SAQRGKFEGRLLVRDRYIRDAFVPVSGAYVGLAAPGDVGSWQREYK 328
S QRG GRLLV DRY+ +P + AYVGLA PGD GSWQRE K
Sbjct: 364 SDQRGNVSGRLLVLDRYVCTDLIPANSAYVGLAPPGDAGSWQRECK 501
Score = 165 bits (417), Expect = 5e-41
Identities = 69/103 (66%), Positives = 86/103 (82%)
Frame = +3
Query: 365 YQCNNIINITSGSEINVGELVYEPPRHGPTLWEIGIPDRSAAEFYVPDPNPLYVNRLYVN 424
++C + GS I +GELVYEPPR GPTLWEIGIPDRSAAEFY PDP+P ++N+L++N
Sbjct: 468 WRCRVMAKRMQGSYIELGELVYEPPRDGPTLWEIGIPDRSAAEFYAPDPSPQHINKLFIN 647
Query: 425 HSDRFRQYGLWERYADLYPNEDLVYTVGISDYKKDWFFAQVTR 467
H DRFRQYGLW+RY++LYP+ DLVYT+G+SDY KDWF+AQ R
Sbjct: 648 HPDRFRQYGLWDRYSELYPDADLVYTIGVSDYTKDWFYAQAPR 776
>TC215981 similar to UP|Q93Z90 (Q93Z90) AT4g24430/T22A6_260, partial (22%)
Length = 425
Score = 127 bits (319), Expect = 1e-29
Identities = 55/62 (88%), Positives = 59/62 (94%)
Frame = +3
Query: 406 AEFYVPDPNPLYVNRLYVNHSDRFRQYGLWERYADLYPNEDLVYTVGISDYKKDWFFAQV 465
AEFYVPDPNP+ +NRLYVNH DRFRQYGLWERYA+LYPNEDLVYTVGISDY+KDWFFAQV
Sbjct: 9 AEFYVPDPNPMCINRLYVNHPDRFRQYGLWERYAELYPNEDLVYTVGISDYRKDWFFAQV 188
Query: 466 TR 467
R
Sbjct: 189 NR 194
>AI973706
Length = 208
Score = 91.7 bits (226), Expect = 7e-19
Identities = 40/66 (60%), Positives = 50/66 (75%)
Frame = +2
Query: 330 YQFWTITDDKGYFSIINVLPGDYNLYSWVNGFIGDYQCNNIINITSGSEINVGELVYEPP 389
YQFWT D+ G+F+I NV PGDYNL++WV GF GDY+ + + ITSGS I + EL Y+PP
Sbjct: 11 YQFWTRADENGFFTIKNVRPGDYNLFAWVPGFHGDYKFGDCMKITSGSYIELSELGYDPP 190
Query: 390 RHGPTL 395
R GPTL
Sbjct: 191 RDGPTL 208
>TC215982 weakly similar to UP|Q6NKW8 (Q6NKW8) At2g22620, partial (13%)
Length = 493
Score = 86.3 bits (212), Expect = 3e-17
Identities = 37/40 (92%), Positives = 39/40 (97%)
Frame = -1
Query: 428 RFRQYGLWERYADLYPNEDLVYTVGISDYKKDWFFAQVTR 467
RFRQYGLWERYA+LYPNEDLVYTVGISDY+KDWFFAQV R
Sbjct: 343 RFRQYGLWERYAELYPNEDLVYTVGISDYRKDWFFAQVNR 224
>BI700921 similar to GP|16323222|gb| AT4g24430/T22A6_260 {Arabidopsis
thaliana}, partial (5%)
Length = 426
Score = 58.5 bits (140), Expect = 6e-09
Identities = 22/28 (78%), Positives = 27/28 (95%)
Frame = +3
Query: 417 YVNRLYVNHSDRFRQYGLWERYADLYPN 444
Y+N+LYVNH D+FRQYGLWERYA+LYP+
Sbjct: 342 YINKLYVNHPDKFRQYGLWERYAELYPD 425
>BQ630617
Length = 425
Score = 57.4 bits (137), Expect = 1e-08
Identities = 26/41 (63%), Positives = 34/41 (82%)
Frame = +2
Query: 1 VVIDNDIVQVNISNPEGCVTGIQYNGLDNLLEINNHESDRG 41
VV++N I++V +SNP G VTGIQYN +DNLLE+ N ES+RG
Sbjct: 260 VVMENGILKVTLSNPGGIVTGIQYNDIDNLLEVLNDESNRG 382
>BE331732 similar to PIR|S25484|S254 ribulose-bisphosphate carboxylase
activase (EC 6.3.4.-) (clone TA1.1) - common tobacco
(fragment), partial (25%)
Length = 388
Score = 37.4 bits (85), Expect = 0.015
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = -1
Query: 443 PNEDLVYTVGISDYKKDWFFAQVTR 467
PN D+V TV +S+Y KDWF AQV R
Sbjct: 166 PNADVVPTVSMSEYIKDWFLAQVKR 92
>TC213795
Length = 631
Score = 32.3 bits (72), Expect = 0.48
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = -3
Query: 386 YEPPRHGPTLWEIGIPDRSAAEFYVPDPNPLYVNRLYVNHSDRFRQYGLWERYADLYPNE 445
+E P+H TL ++ A + + P + N+ YVNHS F + L ++ LYP
Sbjct: 578 HEQPQHDQTL------EKEAPQQHQFHPMSWFPNQEYVNHSIEFSLHLLHQKLLSLYPQR 417
Query: 446 DLV 448
L+
Sbjct: 416 *LI 408
>TC217911 similar to UP|O23605 (O23605) Sperm protein homolog, partial (27%)
Length = 631
Score = 28.9 bits (63), Expect = 5.3
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +1
Query: 408 FYVPDPNPLYVNRLYVNHSDRFRQYGLWERYADLY 442
F++PDPNP + LY RF Q+ RY D +
Sbjct: 85 FHIPDPNPRF*LHLYF----RFSQFNQIIRYLDFF 177
>TC232995
Length = 1009
Score = 28.1 bits (61), Expect = 9.0
Identities = 25/86 (29%), Positives = 41/86 (47%), Gaps = 3/86 (3%)
Frame = +2
Query: 188 YEFRSAGPLKQYLGSHLGPTMLSVFHSTHYSGADLIMKFGENEPWKKVFGPIF--IYLNS 245
+E G LK +LG + T +F + +LI +FG + K + P+ YL+
Sbjct: 284 FEMSMMGELKYFLGLQIKQTQ*GIFINQSKYCKELIKRFGMDSA-KHMSTPMSTNCYLDK 460
Query: 246 NSDGFS-PIKLWEDAKQQMVNEVESW 270
+ G S IK + DA ++V E+ W
Sbjct: 461 DESGQSIDIKQYRDAIGEVV-EIGQW 535
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.326 0.144 0.466
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,694,515
Number of Sequences: 63676
Number of extensions: 341479
Number of successful extensions: 1710
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1700
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1708
length of query: 478
length of database: 12,639,632
effective HSP length: 101
effective length of query: 377
effective length of database: 6,208,356
effective search space: 2340550212
effective search space used: 2340550212
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC148236.5