BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC148236.4 - phase: 0
(279 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229969 UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein... 469 e-133
TC230093 similar to UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-l... 419 e-118
NP501847 unknown [Glycine max] 299 1e-81
TC221359 UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein... 221 2e-66
AW102256 149 1e-36
TC217813 similar to GB|BAB11607.1|10178227|AB025640 ZW10-like pr... 109 2e-24
BG839088 similar to GP|6520148|dbj| ZW10 {Arabidopsis thaliana},... 65 7e-16
TC213860 similar to UP|Q8LGT8 (Q8LGT8) Phosphoglycerate mutase-l... 64 6e-11
TC211557 similar to GB|BAB11607.1|10178227|AB025640 ZW10-like pr... 62 3e-10
BG839089 similar to GP|10178227|dbj ZW10-like protein {Arabidops... 52 2e-07
BI471747 35 0.029
TC221899 30 1.6
TC217131 similar to UP|Q6YKA6 (Q6YKA6) Ubiquitin-conjugating enz... 29 2.7
BG237531 29 2.7
TC219762 28 4.6
BI971913 27 7.8
TC215089 27 7.8
TC212496 27 7.8
>TC229969 UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein, complete
Length = 1133
Score = 469 bits (1208), Expect = e-133
Identities = 224/266 (84%), Positives = 248/266 (93%)
Frame = +1
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQGFHNVEG+KN +AY SYDLFDA+LTPLGWKQVDNLRQHVKASGLSKRIEL
Sbjct: 136 KTLHLVRHAQGFHNVEGEKNFEAYKSYDLFDANLTPLGWKQVDNLRQHVKASGLSKRIEL 315
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCRE 130
VIVSPLLRTMQTAVGVFGG+ +TDG++ PPLMN+NV +S R AISSLN+PPF+AVELCRE
Sbjct: 316 VIVSPLLRTMQTAVGVFGGQPYTDGINVPPLMNDNVGDSGRPAISSLNAPPFIAVELCRE 495
Query: 131 HLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWT 190
HLGVHPCDKRR I++Y++MFPAIDFSLIE DEDILWKPDIREKNEEVAARGLKFLEWLWT
Sbjct: 496 HLGVHPCDKRRNITDYRHMFPAIDFSLIENDEDILWKPDIREKNEEVAARGLKFLEWLWT 675
Query: 191 RKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDESS 250
RKEKEIAVV+HSGFLFH+LSAFGNDCH NVK+EICTHFANCELRS+VIIDRG IGSDESS
Sbjct: 676 RKEKEIAVVTHSGFLFHSLSAFGNDCHPNVKNEICTHFANCELRSMVIIDRGMIGSDESS 855
Query: 251 TNFPGKIPQGLDLPSDVADEKLSGSG 276
TN+PGK+P GLDLPSDVAD+K +G
Sbjct: 856 TNYPGKVPDGLDLPSDVADQKHPENG 933
>TC230093 similar to UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein,
partial (96%)
Length = 1196
Score = 419 bits (1078), Expect = e-118
Identities = 199/264 (75%), Positives = 230/264 (86%)
Frame = +1
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQG HNV G+KN DAY SY+ FDA LT LGW+QV+NLR+HVKASGLSK IEL
Sbjct: 136 KTVHLVRHAQGVHNVAGEKNHDAYNSYEFFDAHLTSLGWEQVNNLRKHVKASGLSKNIEL 315
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVELCRE 130
V++SPLLRTMQTAVGVFGGEA+TDG+ PPLM ENV +S A+SS+N PPF+AVELCRE
Sbjct: 316 VVISPLLRTMQTAVGVFGGEAYTDGIGEPPLMTENVGHSDHPAVSSMNCPPFIAVELCRE 495
Query: 131 HLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLKFLEWLWT 190
+GVHPCDKRR ISEY+NMFPAIDFSLIE DEDILW+ D+REK +EV+ARGLKFLEWLWT
Sbjct: 496 QIGVHPCDKRRTISEYRNMFPAIDFSLIESDEDILWESDVREKTDEVSARGLKFLEWLWT 675
Query: 191 RKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDESS 250
R+EKEIAVV+HS FLF+TL AFGNDCH ++KSEICTHFANCELRS+VIIDRG IGS ES+
Sbjct: 676 REEKEIAVVTHSSFLFNTLRAFGNDCHPDIKSEICTHFANCELRSIVIIDRGMIGSSEST 855
Query: 251 TNFPGKIPQGLDLPSDVADEKLSG 274
TN+PGKIP+G D+PS+ AD SG
Sbjct: 856 TNYPGKIPRGPDVPSEAAD*NHSG 927
>NP501847 unknown [Glycine max]
Length = 942
Score = 299 bits (765), Expect = 1e-81
Identities = 160/257 (62%), Positives = 188/257 (72%), Gaps = 2/257 (0%)
Frame = +1
Query: 11 QTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIEL 70
+T VRHAQGFHNVEG+KN +AY SYDLFDA+LTPLGW QVDNLR+HVKASGLSK+IEL
Sbjct: 46 KTLHLVRHAQGFHNVEGEKNFEAYKSYDLFDANLTPLGWNQVDNLREHVKASGLSKKIEL 225
Query: 71 VIVSPLLRTMQTAVGVFGGEAHTDGVDAPPLMNENVVNSSRHAISSLNSPPFVAVE-LCR 129
VIVSPLLRTMQTAVGVFGGEA+TDG++ PPLMN+NV +S R AISSLN PPF + L R
Sbjct: 226 VIVSPLLRTMQTAVGVFGGEAYTDGINVPPLMNDNVGDSRRPAISSLNVPPFNSSRALPR 405
Query: 130 EHLGVHPCDKRRGISEYQNMFPAIDFSLIERDEDILWKPDIREKNEEVAARGLK-FLEWL 188
GV C +++ + + F + P IREKN +G + F
Sbjct: 406 TFWGVSLCKEKKHHCLPTYVSQLLIFHCYKTMPTFCGNPPIREKNCRSCCQGTEIFGNGC 585
Query: 189 WTRKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEICTHFANCELRSVVIIDRGTIGSDE 248
RK+KE AVV+H GFLFH+L A GNDCH NVK+EICTHFANCELRS+VIID+G IGS+E
Sbjct: 586 GHRKKKEKAVVTHRGFLFHSLRALGNDCHPNVKNEICTHFANCELRSMVIIDKGVIGSNE 765
Query: 249 SSTNFPGKIPQGLDLPS 265
SSTN+ GKIP G PS
Sbjct: 766 SSTNYTGKIPYGRPCPS 816
>TC221359 UP|Q8LGT9 (Q8LGT9) Phosphoglycerate mutase-like protein, partial
(52%)
Length = 1195
Score = 221 bits (562), Expect(2) = 2e-66
Identities = 103/119 (86%), Positives = 112/119 (93%)
Frame = +3
Query: 158 IERDEDILWKPDIREKNEEVAARGLKFLEWLWTRKEKEIAVVSHSGFLFHTLSAFGNDCH 217
IE DEDILWKPDIREKNEEVAARGLKFLEWLWTRKEKEIAVV+HSGFLFH+LSAFGNDCH
Sbjct: 723 IENDEDILWKPDIREKNEEVAARGLKFLEWLWTRKEKEIAVVTHSGFLFHSLSAFGNDCH 902
Query: 218 ANVKSEICTHFANCELRSVVIIDRGTIGSDESSTNFPGKIPQGLDLPSDVADEKLSGSG 276
NVK++ICTHFANCELRS+VIIDRG IGSDESSTN+PGK+P GLDLPSDVAD+K +G
Sbjct: 903 PNVKNKICTHFANCELRSMVIIDRGMIGSDESSTNYPGKVPDGLDLPSDVADQKHPENG 1079
Score = 49.7 bits (117), Expect(2) = 2e-66
Identities = 20/25 (80%), Positives = 24/25 (96%)
Frame = +1
Query: 133 GVHPCDKRRGISEYQNMFPAIDFSL 157
GVHPCDKRR I++Y++MFPAIDFSL
Sbjct: 652 GVHPCDKRRNITDYRHMFPAIDFSL 726
>AW102256
Length = 408
Score = 149 bits (377), Expect = 1e-36
Identities = 71/82 (86%), Positives = 79/82 (95%)
Frame = +1
Query: 16 VRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQVDNLRQHVKASGLSKRIELVIVSP 75
VRHAQGFHNVEG+KN +AY SYDLFDA+LTPLGW QVDNLR+HVKASGLSK+IELVIVSP
Sbjct: 160 VRHAQGFHNVEGEKNFEAYKSYDLFDANLTPLGWNQVDNLREHVKASGLSKKIELVIVSP 339
Query: 76 LLRTMQTAVGVFGGEAHTDGVD 97
LLRTMQTAVGVFGGEA+TDG++
Sbjct: 340 LLRTMQTAVGVFGGEAYTDGIN 405
>TC217813 similar to GB|BAB11607.1|10178227|AB025640 ZW10-like protein
{Arabidopsis thaliana;} , partial (30%)
Length = 863
Score = 109 bits (272), Expect = 2e-24
Identities = 52/84 (61%), Positives = 62/84 (72%)
Frame = -1
Query: 157 LIERDEDILWKPDIREKNEEVAARGLKFLEWLWTRKEKEIAVVSHSGFLFHTLSAFGNDC 216
LI+ +ED WK D+RE EE+AARG KF+ WL TRKEKEIA+V+H L HTLSAFGN
Sbjct: 863 LIDSNEDTWWKADVRETKEELAARGRKFMNWLGTRKEKEIAIVTHRALLLHTLSAFGNYS 684
Query: 217 HANVKSEICTHFANCELRSVVIID 240
H K E+ FANCELRS+VI+D
Sbjct: 683 HPLEKKELSKPFANCELRSMVIVD 612
>BG839088 similar to GP|6520148|dbj| ZW10 {Arabidopsis thaliana}, partial
(16%)
Length = 691
Score = 65.1 bits (157), Expect(2) = 7e-16
Identities = 30/50 (60%), Positives = 38/50 (76%)
Frame = +2
Query: 3 CFSYCGLIQTCMQVRHAQGFHNVEGDKNPDAYLSYDLFDASLTPLGWKQV 52
CF + ++ + VRHAQG HNVEGDKN +AY++ D FDA LTPLGW+QV
Sbjct: 260 CFVHSPIL---LPVRHAQGIHNVEGDKNYNAYINPDYFDAHLTPLGWQQV 400
Score = 35.8 bits (81), Expect(2) = 7e-16
Identities = 17/29 (58%), Positives = 22/29 (75%)
Frame = +1
Query: 50 KQVDNLRQHVKASGLSKRIELVIVSPLLR 78
+QVDNLR V+ SGL I+LVI SP++R
Sbjct: 499 EQVDNLRXMVRDSGLINTIDLVIASPMMR 585
>TC213860 similar to UP|Q8LGT8 (Q8LGT8) Phosphoglycerate mutase-like protein,
partial (15%)
Length = 690
Score = 64.3 bits (155), Expect = 6e-11
Identities = 35/70 (50%), Positives = 41/70 (58%), Gaps = 26/70 (37%)
Frame = +2
Query: 228 FANCELRSVVIIDRG--------------------------TIGSDESSTNFPGKIPQGL 261
FANCELRS+VI+DRG IG ++S+TN+PGKIP GL
Sbjct: 230 FANCELRSMVIVDRG*VCENIHKKKTGFFLS*SRFVCFFSSMIGLEQSTTNYPGKIPSGL 409
Query: 262 DLPSDVADEK 271
DLPSDVADEK
Sbjct: 410 DLPSDVADEK 439
>TC211557 similar to GB|BAB11607.1|10178227|AB025640 ZW10-like protein
{Arabidopsis thaliana;} , partial (20%)
Length = 740
Score = 62.0 bits (149), Expect = 3e-10
Identities = 25/30 (83%), Positives = 30/30 (99%)
Frame = +2
Query: 188 LWTRKEKEIAVVSHSGFLFHTLSAFGNDCH 217
LWT+KEKEIA+V+HSGFLFHTL+AFG+DCH
Sbjct: 644 LWTQKEKEIAIVTHSGFLFHTLNAFGSDCH 733
Score = 43.1 bits (100), Expect = 1e-04
Identities = 17/27 (62%), Positives = 21/27 (76%)
Frame = +2
Query: 161 DEDILWKPDIREKNEEVAARGLKFLEW 187
DED WK ++RE EE+AARG+KFL W
Sbjct: 2 DEDTWWKANVRETKEELAARGMKFLNW 82
>BG839089 similar to GP|10178227|dbj ZW10-like protein {Arabidopsis
thaliana}, partial (12%)
Length = 616
Score = 52.4 bits (124), Expect = 2e-07
Identities = 27/36 (75%), Positives = 27/36 (75%)
Frame = -1
Query: 97 DAPPLMNENVVNSSRHAISSLNSPPFVAVELCREHL 132
D PLM N NS R AISSLNSPP VAVELCREHL
Sbjct: 610 DVLPLMVANAGNSYRAAISSLNSPPVVAVELCREHL 503
>BI471747
Length = 421
Score = 35.4 bits (80), Expect = 0.029
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +3
Query: 11 QTCMQVRHAQGFHNVEGDKNPDA 33
+T +QVRH QG HNVEGDK+ +A
Sbjct: 351 KTRIQVRHGQGIHNVEGDKDYNA 419
>TC221899
Length = 1292
Score = 29.6 bits (65), Expect = 1.6
Identities = 11/29 (37%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = +2
Query: 13 CMQVRHAQGFHNVEGDKNP-DAYLSYDLF 40
C+ + ++QGF N+ G +NP D Y+++ F
Sbjct: 572 CLCISYSQGFFNLNGHENPQDGYIAFYFF 658
>TC217131 similar to UP|Q6YKA6 (Q6YKA6) Ubiquitin-conjugating enzyme E2,
partial (62%)
Length = 866
Score = 28.9 bits (63), Expect = 2.7
Identities = 10/36 (27%), Positives = 20/36 (54%)
Frame = -3
Query: 189 WTRKEKEIAVVSHSGFLFHTLSAFGNDCHANVKSEI 224
W ++ ++ + +HS FL+H + + CH + S I
Sbjct: 690 WNKEHRKQVI*NHSAFLWHCCKSRVHSCHGLIPSNI 583
>BG237531
Length = 274
Score = 28.9 bits (63), Expect = 2.7
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 10/56 (17%)
Frame = -2
Query: 92 HTDGVDAPPLMNENVV----------NSSRHAISSLNSPPFVAVELCREHLGVHPC 137
H ++ PLMNEN+V + H +L+ +AVE C H VH C
Sbjct: 270 HMSPPNSQPLMNENIVVAVVVAAADDDEVEHEAETLHPQTLLAVEHCYSH--VHNC 109
>TC219762
Length = 598
Score = 28.1 bits (61), Expect = 4.6
Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 10/62 (16%)
Frame = -1
Query: 224 ICTHFA---NCELRSVVIIDRGTIGS-------DESSTNFPGKIPQGLDLPSDVADEKLS 273
+C F NC + ++++R T+ S D + +NF + Q LPS+ AD S
Sbjct: 211 VCLKFTSERNCSSQESIVLNRFTLLSMPASTEVDATKSNFDRDVIQSTCLPSNTADTTAS 32
Query: 274 GS 275
S
Sbjct: 31 AS 26
>BI971913
Length = 426
Score = 27.3 bits (59), Expect = 7.8
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +1
Query: 150 FPAIDFSLIERDEDILW 166
F +IDF L+E+DE LW
Sbjct: 316 FLSIDFDLVEKDEGFLW 366
>TC215089
Length = 802
Score = 27.3 bits (59), Expect = 7.8
Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +3
Query: 216 CHANVKSEICTHFANCELRSVVIIDRGTIGSDESSTNF-----PGKIPQGLDLPSDV 267
C+ + S IC+H + R+ R T G STNF G+ P+G D +V
Sbjct: 75 CNFSSTSCICSHSHSFRTRATSYTFRTTKGRATKSTNFNFGATKGRAPKGTDYNFEV 245
>TC212496
Length = 608
Score = 27.3 bits (59), Expect = 7.8
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = +3
Query: 150 FPAIDFSLIERDEDILW 166
F +IDF L+E+DE LW
Sbjct: 150 FLSIDFDLVEKDEGFLW 200
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.136 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,148,765
Number of Sequences: 63676
Number of extensions: 205044
Number of successful extensions: 991
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 986
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 987
length of query: 279
length of database: 12,639,632
effective HSP length: 96
effective length of query: 183
effective length of database: 6,526,736
effective search space: 1194392688
effective search space used: 1194392688
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC148236.4