BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147963.2 - phase: 0
(129 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC228247 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific le... 147 1e-36
TC205690 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family prote... 74 2e-14
TC206024 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific le... 64 3e-11
TC217152 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-li... 63 4e-11
TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family prote... 62 1e-10
TC217153 similar to GB|AAS76702.1|45773796|BT012215 At1g56240 {A... 52 1e-07
TC228248 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific le... 44 2e-05
BG839963 weakly similar to GP|21592660|gb| phloem-specific lecti... 44 2e-05
TC232377 weakly similar to UP|O81865 (O81865) Lectin like protei... 40 4e-04
TC221119 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-li... 30 0.013
TC233929 weakly similar to UP|O80811 (O80811) T8F5.18 protein, p... 31 0.14
AW306424 26 4.5
TC226500 similar to UP|Q9LFG2 (Q9LFG2) Diaminopimelate epimerase... 26 4.5
BU760700 26 5.9
TC226499 similar to UP|Q9LFG2 (Q9LFG2) Diaminopimelate epimerase... 25 7.7
>TC228247 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (46%)
Length = 1124
Score = 147 bits (371), Expect = 1e-36
Identities = 78/128 (60%), Positives = 92/128 (70%), Gaps = 1/128 (0%)
Frame = +3
Query: 3 THILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKV-QNGMAYLYNKYEDTMFYEN 61
T ILTPNT+YVVYLI K SHR YGLDS EVS+A+ NKV Q+G YL +D EN
Sbjct: 510 TRILTPNTSYVVYLIMKTSHREYGLDSVACEVSIAVDNKVKQSGRVYLCQNEKD----EN 677
Query: 62 HRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEVGYRLKGGLIV 121
+ K E I +P +R+DGWMEIE+GEFFCGE D EV MS+MEVGY+LKGGLIV
Sbjct: 678 NLKKE---------SIGIPMRREDGWMEIEMGEFFCGEADEEVLMSLMEVGYQLKGGLIV 830
Query: 122 EGIEVRPK 129
EG+E+RPK
Sbjct: 831 EGVEIRPK 854
>TC205690 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family protein-like,
partial (33%)
Length = 1360
Score = 73.9 bits (180), Expect = 2e-14
Identities = 48/130 (36%), Positives = 71/130 (53%), Gaps = 1/130 (0%)
Frame = +2
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
+ T +L+PNT Y YL+ K+ G + P E+SV + + + E+
Sbjct: 632 LNTLVLSPNTQYAAYLVFKMID-ARGFHNRPVELSVNVFGGHGSTKIVCLDPNEELP--- 799
Query: 61 NHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEV-GYRLKGGL 119
HR++E ++ P+ R DGW+EIE+GEFF +D EV+MSV+E G K GL
Sbjct: 800 -HRRVEG---------LQRPNARSDGWLEIEMGEFFNTGLDDEVQMSVVETKGGNWKSGL 949
Query: 120 IVEGIEVRPK 129
+EGIEV+PK
Sbjct: 950 FIEGIEVKPK 979
>TC206024 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (47%)
Length = 954
Score = 63.5 bits (153), Expect = 3e-11
Identities = 47/133 (35%), Positives = 67/133 (50%), Gaps = 4/133 (3%)
Frame = +3
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYE--DTMF 58
+ T L+ NT Y +L+ K+ + G P +SV + N KY D
Sbjct: 438 INTLTLSSNTHYATFLVFKMIN-ASGFHYHPTVLSVGVLGGNSN------TKYVCLDPNL 596
Query: 59 YENHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEVGYRL-K 116
NHR +E++ P R DGW+EIE+GEFF G+ + +V+M VME + K
Sbjct: 597 KGNHRL----------QELQFPKVRSDGWLEIEMGEFFNSGQEEKQVQMKVMETTSHIWK 746
Query: 117 GGLIVEGIEVRPK 129
G I+EGIE+RPK
Sbjct: 747 CGFILEGIEIRPK 785
>TC217152 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like protein,
partial (8%)
Length = 909
Score = 62.8 bits (151), Expect = 4e-11
Identities = 42/130 (32%), Positives = 66/130 (50%), Gaps = 1/130 (0%)
Frame = +3
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
++T +L+PNT Y +L+ ++ G PA +SV+ + +
Sbjct: 315 IKTRVLSPNTQYAAFLVFQMID-ASGFHHHPAMLSVSNVGGSRTSKYVCLDP-------- 467
Query: 61 NHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEVGYRLKGGL 119
+E N L + + ++ P+ R D W+EIE+GEFF G + E+ M+V E K G
Sbjct: 468 ---NLEDNDLDDRFRGLQRPNVRKDKWLEIEMGEFFNSGLEEDEIYMNVRETSDMWKHGF 638
Query: 120 IVEGIEVRPK 129
I+EGIEVRPK
Sbjct: 639 ILEGIEVRPK 668
>TC230781 weakly similar to UP|Q6K3E9 (Q6K3E9) F-box family protein-like,
partial (29%)
Length = 971
Score = 61.6 bits (148), Expect = 1e-10
Identities = 45/132 (34%), Positives = 68/132 (51%), Gaps = 3/132 (2%)
Frame = +2
Query: 1 MRTHILTPNTTYVVYLITKVSHRVYGLDSAPAEVSVAMANKVQNGMAYLYNKYEDTMFYE 60
M T +L+PNT Y +L+ K+ G PA +SV + G + N D +
Sbjct: 383 MNTRVLSPNTNYAAFLVFKMID-AGGFHYDPAVLSVG----ILGGNSSTKNVCLDPNLVD 547
Query: 61 NHRKMERNKLMEDNKEIRVPSKRDDGWMEIELGEFF---CGEVDMEVKMSVMEVGYRLKG 117
N +L + ++ P+ R DGW+EIE+GEFF E ++++K+S + K
Sbjct: 548 N-------RLDDRFHGLQRPTVRSDGWLEIEMGEFFNPGLEEDELQIKVSETTSNW-WKR 703
Query: 118 GLIVEGIEVRPK 129
G I+EGIEVRPK
Sbjct: 704 GFILEGIEVRPK 739
>TC217153 similar to GB|AAS76702.1|45773796|BT012215 At1g56240 {Arabidopsis
thaliana;} , partial (13%)
Length = 635
Score = 51.6 bits (122), Expect = 1e-07
Identities = 27/51 (52%), Positives = 34/51 (65%), Gaps = 1/51 (1%)
Frame = +1
Query: 80 PSKRDDGWMEIELGEFF-CGEVDMEVKMSVMEVGYRLKGGLIVEGIEVRPK 129
P+ R D W+EIE+GEFF G + E+ M+V E K G I+EGIEVRPK
Sbjct: 424 PNVRKDKWLEIEMGEFFNSGLEEDEIYMNVRETSDMWKHGFILEGIEVRPK 576
>TC228248 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (28%)
Length = 519
Score = 44.3 bits (103), Expect = 2e-05
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +3
Query: 3 THILTPNTTYVVYLITKVSHRVYGL 27
T ILTPNT+YVVYLI K SHR YGL
Sbjct: 183 TRILTPNTSYVVYLIMKTSHREYGL 257
Score = 29.3 bits (64), Expect = 0.53
Identities = 19/53 (35%), Positives = 28/53 (51%), Gaps = 2/53 (3%)
Frame = +3
Query: 79 VPSKRDDGWMEIE--LGEFFCGEVDMEVKMSVMEVGYRLKGGLIVEGIEVRPK 129
V R W+EI+ +G V +M+ +R + GLIVEG+E+RPK
Sbjct: 132 VAELRTVSWLEIQGKIGTRILTPNTSYVVYLIMKTSHR-EYGLIVEGVEIRPK 287
>BG839963 weakly similar to GP|21592660|gb| phloem-specific lectin PP2-like
protein {Arabidopsis thaliana}, partial (11%)
Length = 707
Score = 44.3 bits (103), Expect = 2e-05
Identities = 24/54 (44%), Positives = 33/54 (60%), Gaps = 5/54 (9%)
Frame = +3
Query: 80 PSKRDDGWMEIELGEFF----CGEVDMEVKMSVMEV-GYRLKGGLIVEGIEVRP 128
P +R DGWME+ELG+ F E + EV++ V EV K G+ V+G E+RP
Sbjct: 351 PKERSDGWMEVELGDXFNVGGXKEKEKEVEIGVYEVXSGGWKXGIFVQGXEIRP 512
>TC232377 weakly similar to UP|O81865 (O81865) Lectin like protein
(AT4g19840/T16H5_200), partial (16%)
Length = 531
Score = 39.7 bits (91), Expect = 4e-04
Identities = 21/57 (36%), Positives = 33/57 (57%), Gaps = 3/57 (5%)
Frame = +1
Query: 76 EIRVPSKRDDGWMEIELGEFFCGEVD---MEVKMSVMEVGYRLKGGLIVEGIEVRPK 129
++ + K + WMEI +GEF E D ME+ M E G K GL+++G+ ++PK
Sbjct: 13 KVNLNEKLRESWMEILVGEFVASEKDAGEMEISMYEYE-GGMWKTGLVIQGVVIKPK 180
>TC221119 similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like protein,
partial (6%)
Length = 971
Score = 30.0 bits (66), Expect(2) = 0.013
Identities = 12/14 (85%), Positives = 14/14 (99%)
Frame = +2
Query: 116 KGGLIVEGIEVRPK 129
KGGLIV+GIE+RPK
Sbjct: 566 KGGLIVDGIEIRPK 607
Score = 23.5 bits (49), Expect(2) = 0.013
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +1
Query: 84 DDGWMEIELGEFF 96
D+ W EIELG F+
Sbjct: 499 DEEWSEIELGSFY 537
>TC233929 weakly similar to UP|O80811 (O80811) T8F5.18 protein, partial (10%)
Length = 509
Score = 31.2 bits (69), Expect = 0.14
Identities = 18/51 (35%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
Frame = +3
Query: 81 SKRDDGWMEIELGEFFCGEVDM-EVKMSVMEV-GYRLKGGLIVEGIEVRPK 129
+K + + I +GEF E D+ +++S+ E G K GLI++GI ++PK
Sbjct: 126 NKLRESXIHIPVGEFVASEKDVGNIEISMYEYEGGMWKTGLIIQGIVIKPK 278
>AW306424
Length = 174
Score = 26.2 bits (56), Expect = 4.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 16 LITKVSHRVYGLDSAPAEVSVAMANKVQNGMA 47
+ T + H+VYGL++ A S M + + G+A
Sbjct: 28 MATSLPHKVYGLEAVKAVFSKLMESSEKGGIA 123
>TC226500 similar to UP|Q9LFG2 (Q9LFG2) Diaminopimelate epimerase-like
protein (AT3g53580/F4P12_280), partial (76%)
Length = 1379
Score = 26.2 bits (56), Expect = 4.5
Identities = 11/46 (23%), Positives = 25/46 (53%)
Frame = +3
Query: 79 VPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEVGYRLKGGLIVEGI 124
+P +DG + +++GE +D+ K+ + +K L+V+G+
Sbjct: 588 IPEVLEDGNVRVDMGEPVLKALDVPTKLPANKDNAVVKSQLVVDGV 725
>BU760700
Length = 445
Score = 25.8 bits (55), Expect = 5.9
Identities = 17/57 (29%), Positives = 28/57 (48%), Gaps = 3/57 (5%)
Frame = +3
Query: 76 EIRVPSKRDDGWMEIELGEFFCGEVDMEVKMSV---MEVGYRLKGGLIVEGIEVRPK 129
E+RV + DD + +ELG F DM ++V +G+ + L + G+ V K
Sbjct: 15 EVRVLRQSDDDHLVLELGMNFLTADDMSAILAVKLRKRLGFGMWAKLHITGMHVEGK 185
>TC226499 similar to UP|Q9LFG2 (Q9LFG2) Diaminopimelate epimerase-like
protein (AT3g53580/F4P12_280), partial (60%)
Length = 830
Score = 25.4 bits (54), Expect = 7.7
Identities = 11/46 (23%), Positives = 24/46 (51%)
Frame = +1
Query: 79 VPSKRDDGWMEIELGEFFCGEVDMEVKMSVMEVGYRLKGGLIVEGI 124
+P +DG + +++GE D+ K+ + +K L+V+G+
Sbjct: 130 IPEVLEDGNVRVDMGEPILKASDVPTKLHANKDNAVVKSELVVDGV 267
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,500,944
Number of Sequences: 63676
Number of extensions: 45389
Number of successful extensions: 258
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 249
length of query: 129
length of database: 12,639,632
effective HSP length: 86
effective length of query: 43
effective length of database: 7,163,496
effective search space: 308030328
effective search space used: 308030328
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147963.2