BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147875.3 + phase: 0
(293 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC213218 145 3e-35
TC233660 99 3e-21
AI496251 60 1e-09
TC209421 45 3e-05
TC220292 weakly similar to UP|O94096 (O94096) Kexin, partial (4%) 45 4e-05
TC222982 UP|Q9SJF7 (Q9SJF7) T27G7.3, partial (5%) 42 4e-04
BE661471 weakly similar to PIR|A86318|A86 protein F15H18.11 [imp... 41 6e-04
TC211703 38 0.005
TC234535 similar to UP|Q9S960 (Q9S960) LEAUX=ARABIDOPSIS auxin-r... 37 0.014
TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier pr... 33 0.15
CA853663 weakly similar to PIR|A86318|A86 protein F15H18.11 [imp... 33 0.15
TC207546 similar to UP|Q6YR38 (Q6YR38) ABC-type cobalt transport... 31 0.58
CA936237 30 0.98
TC211136 UP|Q84VI3 (Q84VI3) Envelope-like protein (Fragment), co... 30 0.98
TC225701 similar to UP|Q41041 (Q41041) Outer envelope membrane p... 29 2.2
TC219365 similar to UP|Q6IEM2 (Q6IEM2) WRKY transcription factor... 28 3.7
TC203804 homologue to UP|Q6VAF9 (Q6VAF9) Alpha-tubulin 4, complete 28 3.7
CA784474 homologue to SP|Q39261|ZFP2_ Zinc finger protein 2. [Mo... 28 3.7
TC220174 28 4.9
AW277953 similar to GP|10441016|gb alpha-tubulin {Oryza sativa},... 28 6.4
>TC213218
Length = 835
Score = 145 bits (365), Expect = 3e-35
Identities = 81/228 (35%), Positives = 122/228 (52%), Gaps = 2/228 (0%)
Frame = +1
Query: 40 FDCANLKNLRYPFWGSSRPQYCGHPSFELQCKDEL-ASITIMSQNYKILEVSDSDHRLKV 98
F+C ++ L YPF G RP +CG P F L C++ + A + I S +Y+++++ DH L +
Sbjct: 169 FNCGSITILSYPFTGGDRPSFCGPPQFLLNCRNGVVAELNISSVSYRVIDIDSEDHTLTL 348
Query: 99 VRTDYWNNVCPTSLKNTTLDCTFFDYGSDSHNLTLYYDCPHTPFLMPDSFSPQFNCSING 158
R D WN C N+T D F YGS + NLTL+Y+C T ++ ++ FNC NG
Sbjct: 349 ARLDLWNETCTDVYVNSTFDGPVFSYGSGNQNLTLFYECKPTSRII-ETPENLFNCWSNG 525
Query: 159 TQMVNYFMLESVADNAESLDSFSETLRTCKSRVVVPFLESEAERVATNSSVENLKDVIDN 218
+ +Y ++ LD E + C V VP L+ +A+R+ N S+ L +V+
Sbjct: 526 DKNNSYSLV-----GPFPLDPILEVVE-CDEHVKVPILKVQADRLVENRSL--LGEVLMK 681
Query: 219 GFGVEW-NANNSLCHECQSSGGHCGYESSSREFTCFCKDGSFPHSCSS 265
GF V + N S C EC SGG CG++S + E C C D H C++
Sbjct: 682 GFNVNYMNPYESECFECLDSGGVCGFDSDNDEHICICGD----HLCAT 813
>TC233660
Length = 1039
Score = 98.6 bits (244), Expect = 3e-21
Identities = 65/244 (26%), Positives = 108/244 (43%), Gaps = 13/244 (5%)
Frame = +3
Query: 42 CANLKNLRYPFWGSSRP---QYCGHPSFELQCKDELASITIMSQNYKILEVSDSDHRLKV 98
C NL N+ YPFW S ++CG+P F L+C D+ A + + Y++ +++ H + +
Sbjct: 96 CGNL-NISYPFWKKSNTNVQEFCGYPEFGLECLDDQAIMIFPTDRYQVTDINYDIHSITL 272
Query: 99 VRTDYWNNVCPTSLKNTTLDCTFFDYGSDSHNLTLYYDCPHTPFLMPDSFSPQFNCSING 158
+ D CP + N +L + S NL+ Y++C P S C +
Sbjct: 273 LDIDVLGQPCPRARHNVSLHNLPLSFSSLDFNLSFYFNCSSYP-----SSIQHIGCMEHD 437
Query: 159 TQMVNYFMLESVADNAESLDSFSETLRTCKSRVVVPFLESEAERVATNSSVENLKDVIDN 218
F D AES + + LR C+ VVV + E E +S + D +
Sbjct: 438 KYQSYVF---KTGDEAES--NGFDWLRHCEEHVVVTVKQDEIE---ISSLITGFGDAMQK 593
Query: 219 GFGVEWNANNSLCHECQSSGGHCGYESSSREFTCFCKDG-SFPHSCSSG---------EH 268
GF ++W C C+ S G+C ++ ++++ C C DG + SC G +
Sbjct: 594 GFVLDWMRAQD-CAVCEESNGYCRFDQATKQSRCLCSDGRTEAKSCKKGTR*LI*SLLKK 770
Query: 269 HCRF 272
+CRF
Sbjct: 771 YCRF 782
>AI496251
Length = 226
Score = 60.1 bits (144), Expect = 1e-09
Identities = 25/57 (43%), Positives = 37/57 (64%), Gaps = 2/57 (3%)
Frame = +3
Query: 36 CSSL-FDCANLKNLRYPFWGSSRPQYCGHPSFELQC-KDELASITIMSQNYKILEVS 90
CS L F C L+N+ YPFWG +RPQ+CG F+L C D+ S+ + SQ + +L ++
Sbjct: 51 CSQLSFSCGTLRNISYPFWGGNRPQFCGRNGFKLTCMHDQNTSVQVGSQRFNVLNIN 221
>TC209421
Length = 1028
Score = 45.4 bits (106), Expect = 3e-05
Identities = 29/123 (23%), Positives = 54/123 (43%), Gaps = 1/123 (0%)
Frame = +3
Query: 13 ITTIILIKIPTSSCADNLQQYNKCSSLFDCANLKNLRYPFWGSSRPQYCGHPSFELQCKD 72
+ I+L+ + CA Q + L C + N+ YPF P+ CG +EL C++
Sbjct: 12 LLVILLLLLVQQICATKKQDHG--CPLSSCGKITNITYPFRLKGHPKSCGDNRYELACEN 185
Query: 73 ELASITIMSQNYKILEVSDSDHRLKVVRTDYWNNVCPTSLKNTTLD-CTFFDYGSDSHNL 131
+ + + S Y + ++ ++ ++VV +SL L F D +D +N+
Sbjct: 186 NVTVLHLYSGKYHVQAINYNNFTIRVVDPGVDQQTNCSSLPRYFLSRSNFTDTYNDRYNM 365
Query: 132 TLY 134
Y
Sbjct: 366 DPY 374
>TC220292 weakly similar to UP|O94096 (O94096) Kexin, partial (4%)
Length = 1094
Score = 45.1 bits (105), Expect = 4e-05
Identities = 63/277 (22%), Positives = 103/277 (36%), Gaps = 20/277 (7%)
Frame = +3
Query: 1 MQLLHPSIFSFLITTIILIKIPTSSCADNLQQYNKCSSLFDCANLKNLRYPFWGSSRPQY 60
M+ + +F F T ++L+ L + SS C N PF S+ P
Sbjct: 267 MESMTTRLFLFAGTLLLLL----------LSRATHVSSATLCPPCGNTTVPFPLSTTPT- 413
Query: 61 CGHPSFELQCKDELASI-TIMSQNYKILEVSDSDHRLKVVRTDYWNNVCPTSLK------ 113
CG PS++++C + ++ +Y I + + R + N C ++ K
Sbjct: 414 CGDPSYKIRCSSSNTLVFDTLNNSYPIESIDPNSQRFVIRPAPLLTNTCVSTDKVHQGIQ 593
Query: 114 -NTTLDCTFFDYGSDSHNLTLYYDCPHTPFLMPDSFSPQFNCSINGTQMVNYFMLESVAD 172
NTTL + S N +Y +C T P NCS + +Y + A
Sbjct: 594 LNTTL-----PFNITSSNTIVYLNCTTTLLQSP------LNCSA-ASACHSYIKATASAA 737
Query: 173 NAESLDSFSETLRTCKSRVVVPFLESEAERVATNSSVENLKDVI------DNGFGVEW-N 225
+ T RT S L + SS NL + + G ++W +
Sbjct: 738 ACQGAGPLCCTYRTGGSSNSY-MLRVRDSGCSAYSSFVNLNPALPVNRWPEPGLEIQWLS 914
Query: 226 ANNSLC---HECQSSGGHCGYESSSR--EFTCFCKDG 257
++C +C S+ CG ++SS CFC DG
Sbjct: 915 PKETVCGSQQDCDSATSTCGPDASSALGIRRCFCNDG 1025
>TC222982 UP|Q9SJF7 (Q9SJF7) T27G7.3, partial (5%)
Length = 1161
Score = 41.6 bits (96), Expect = 4e-04
Identities = 59/257 (22%), Positives = 92/257 (34%), Gaps = 33/257 (12%)
Frame = +1
Query: 31 QQYNKCSSLFDCANLKNLRYPFWGSSRPQYCGHPSFELQCKDELASITIMSQNYKILEVS 90
QQ+ C C + N+ +PF P +CG ++L C + +T+ S Y + ++
Sbjct: 229 QQHQPCPPS-SCGIIGNISFPFRLKDDPSHCGDTRYQLDCVNNATLLTLFSGKYHVQDID 405
Query: 91 DSDHRLKV-------------VRTDYWN-NVCPTSLKNTTLDCTFFDYGSDSHNLTL--- 133
+++KV R W+ N PT L D+GS L L
Sbjct: 406 YKRYKIKVSDAGAVEDSNCFIPRNFLWDGNFRPTGLVGPD------DFGSQPFVLDLDFT 567
Query: 134 ---YYDCPH----TPFLMPDSFSPQFNCSINGTQMVNYFMLE------SVADNAESLDSF 180
Y++C + P + + C+ G V Y +LE V D D
Sbjct: 568 RIGYFNCANPVTGDPRYVKVDTTHTSRCATQGQGHV-YALLEPSYLEYRVKDITVGCDLM 744
Query: 181 SETLRTCKSRVVVPFLESEAERVATNSSVENLKDVIDNGFGVEWNANNSLCHECQSSGGH 240
TL + SEAE+ N + + + I GF + W +C G
Sbjct: 745 VATLAN--------WSLSEAEK--GNFTYDEIHKRIIEGFQLSWLP--VICENRCGKGTK 888
Query: 241 CGYESSSREFTC---FC 254
C S+ E C FC
Sbjct: 889 CVINESNGEVQCDRHFC 939
>BE661471 weakly similar to PIR|A86318|A86 protein F15H18.11 [imported] -
Arabidopsis thaliana, partial (4%)
Length = 662
Score = 41.2 bits (95), Expect = 6e-04
Identities = 28/102 (27%), Positives = 46/102 (44%), Gaps = 6/102 (5%)
Frame = +2
Query: 42 CANLKNLRYPFW--GSSRPQYCGHPSFELQCK-DELASITIMSQNYKILEVSDSDHRLKV 98
C N +N+ YPF+ G +P +CG P FEL C + +T+M Y I ++ + L+V
Sbjct: 116 CGNGQNISYPFYIQGKQKP-FCGQPGFELTCSHNGFPILTLMYTPYTIHQIFYDNQSLRV 292
Query: 99 VRTDYWN---NVCPTSLKNTTLDCTFFDYGSDSHNLTLYYDC 137
+ + C +N T+ F + L + Y C
Sbjct: 293 SNPVFSQPNLSSCIAPTQNLTVGRYRFRVAPNQRELFVLYGC 418
>TC211703
Length = 683
Score = 38.1 bits (87), Expect = 0.005
Identities = 26/92 (28%), Positives = 42/92 (45%)
Frame = +3
Query: 42 CANLKNLRYPFWGSSRPQYCGHPSFELQCKDELASITIMSQNYKILEVSDSDHRLKVVRT 101
C + N+ YPF P+ CG +EL C++ + + S Y + ++ ++ ++VV
Sbjct: 30 CGKITNITYPFRLRGDPKGCGDNRYELACENNVTVLYSYSGKYHVQAINYNNFTIRVVDP 209
Query: 102 DYWNNVCPTSLKNTTLDCTFFDYGSDSHNLTL 133
C +SL L T F SDS N L
Sbjct: 210 GVQQPNC-SSLPRYFLSPTNF---SDSDNFYL 293
>TC234535 similar to UP|Q9S960 (Q9S960) LEAUX=ARABIDOPSIS auxin-regulated
protein homolog (Fragment), partial (11%)
Length = 530
Score = 36.6 bits (83), Expect = 0.014
Identities = 30/114 (26%), Positives = 51/114 (44%), Gaps = 14/114 (12%)
Frame = +3
Query: 44 NLKNLRYPFW--GSSRPQYCGHPSFELQC-KDELASITIMSQNYKILEVSDSDHRLKVVR 100
N +++ YPF+ G P +CG+P F + C + + + + Y I ++ + L+V
Sbjct: 93 NNQSISYPFYIQGKQEP-FCGNPGFGISCGPNGFPILNLSNTQYIIHQIFYENQTLRVSN 269
Query: 101 TDY---------WNNVCPTSL-KNTTLDCT-FFDYGSDSHNLTLYYDCPHTPFL 143
T + P L +N TL T FD + N+ L+Y C P+L
Sbjct: 270 TAFSVSRPNTTNSKGCLPLPLTQNLTLPSTREFDIAPNQTNIRLFYGCGSLPWL 431
>TC227967 homologue to UP|Q84WX3 (Q84WX3) Auxin efflux carrier protein PIN1,
partial (56%)
Length = 1532
Score = 33.1 bits (74), Expect = 0.15
Identities = 23/94 (24%), Positives = 31/94 (32%), Gaps = 24/94 (25%)
Frame = +1
Query: 218 NGFGVE----WNANNSLCHECQ--------SSGGHCGYESSSREFTCFCKDGSFPHSCS- 264
+GF E W ++S CH C+ S HC + C C S P +C
Sbjct: 862 HGFATEGHSMWKFHSSFCHGCEIPYRSSCHGSCFHCCWTQRCSPTRCHCSGSSSPRNCPI 1041
Query: 265 -----------SGEHHCRFHCCVMFLHSIMSFHL 287
+H C F F H+ HL
Sbjct: 1042CLC*GI*CTS*YSQHSCYFWDADCFAHNSSVLHL 1143
>CA853663 weakly similar to PIR|A86318|A86 protein F15H18.11 [imported] -
Arabidopsis thaliana, partial (4%)
Length = 435
Score = 33.1 bits (74), Expect = 0.15
Identities = 18/56 (32%), Positives = 32/56 (57%), Gaps = 3/56 (5%)
Frame = +2
Query: 46 KNLRYPFW--GSSRPQYCGHPSFELQC-KDELASITIMSQNYKILEVSDSDHRLKV 98
+N+ YPF+ G +P +CG P FEL C + +T+M Y I ++ ++ L++
Sbjct: 146 QNISYPFYIQGKQKP-FCGQPGFELTCGHNGFPILTLMYTPYTIHQIFYHNNSLRL 310
>TC207546 similar to UP|Q6YR38 (Q6YR38) ABC-type cobalt transport system,
permease component, partial (5%)
Length = 1389
Score = 31.2 bits (69), Expect = 0.58
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = +3
Query: 1 MQLLHPSIFSFLITTIILIKIPTSSCADNLQQYNKCSSLFDCANLKNLRYPFW 53
+++LH + FL+TT I +PT S N S C L + FW
Sbjct: 117 LEILHLMLIYFLVTTNIRATLPTFSSPHNKVFRKNISDFIACHQLHTFFFCFW 275
>CA936237
Length = 458
Score = 30.4 bits (67), Expect = 0.98
Identities = 17/41 (41%), Positives = 18/41 (43%)
Frame = +2
Query: 228 NSLCHECQSSGGHCGYESSSREFTCFCKDGSFPHSCSSGEH 268
NSL HE + G CG S F CFC G C G H
Sbjct: 44 NSLVHEMKRLGLTCG---SLE*FYCFCNSGFLTIECCIGIH 157
>TC211136 UP|Q84VI3 (Q84VI3) Envelope-like protein (Fragment), complete
Length = 2043
Score = 30.4 bits (67), Expect = 0.98
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Frame = +3
Query: 226 ANNSLCHECQSSGGHCGY-ESSSREFTCFCKDGSFPHSCSSGEHHCRFHCCVMF 278
A+N + CQ+S E+ SR+ C + SC G H FHCC F
Sbjct: 1335 ASNCQGNHCQTSPALAKEREAFSRKAKCEVCHSAQDWSCKLGSHQSYFHCCHRF 1496
>TC225701 similar to UP|Q41041 (Q41041) Outer envelope membrane protein,
partial (68%)
Length = 517
Score = 29.3 bits (64), Expect = 2.2
Identities = 27/97 (27%), Positives = 33/97 (33%), Gaps = 9/97 (9%)
Frame = -3
Query: 198 SEAERVATNSSVENLKDVIDNGF------GVEWNA---NNSLCHECQSSGGHCGYESSSR 248
S VA + + +V+ GF GV W N S C G G+ES
Sbjct: 263 SSGRHVAGGFRIGGVGNVVSGGFRKSIVVGVTWRVGVVNRSTCL------GKEGFESELY 102
Query: 249 EFTCFCKDGSFPHSCSSGEHHCRFHCCVMFLHSIMSF 285
S P C HH RF C V F + F
Sbjct: 101 ---------SEPREC*GSRHHRRFFCRVFFFFFFLFF 18
>TC219365 similar to UP|Q6IEM2 (Q6IEM2) WRKY transcription factor 59, partial
(33%)
Length = 788
Score = 28.5 bits (62), Expect = 3.7
Identities = 33/128 (25%), Positives = 53/128 (40%)
Frame = +1
Query: 111 SLKNTTLDCTFFDYGSDSHNLTLYYDCPHTPFLMPDSFSPQFNCSINGTQMVNYFMLESV 170
SL +T L T DY + N YY H+ + S S +F S +Y +LE
Sbjct: 28 SLSDTYLAITSMDYYFGNLNPNPYYH--HSAVVNMASPSSEFMLS-------DYLVLEDA 180
Query: 171 ADNAESLDSFSETLRTCKSRVVVPFLESEAERVATNSSVENLKDVIDNGFGVEWNANNSL 230
+S+S++ E+E+ AT+S + GFG + N ++
Sbjct: 181 LVVDHHQESWSQST------------ETESSEKATSSDASH-------GFGDATSTNTNM 303
Query: 231 CHECQSSG 238
+CQ+SG
Sbjct: 304 HIKCQNSG 327
>TC203804 homologue to UP|Q6VAF9 (Q6VAF9) Alpha-tubulin 4, complete
Length = 1692
Score = 28.5 bits (62), Expect = 3.7
Identities = 15/49 (30%), Positives = 21/49 (42%), Gaps = 5/49 (10%)
Frame = -3
Query: 233 ECQSSGGHCGYESSSREFTCFCKDGSF-----PHSCSSGEHHCRFHCCV 276
E Q G C +++ RE C C H C+ E+ R HCC+
Sbjct: 829 EKQGG*GWCRWDAQCRESVCRCHKWLHCPRGQQHRCAPRENGWRGHCCM 683
>CA784474 homologue to SP|Q39261|ZFP2_ Zinc finger protein 2. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (14%)
Length = 423
Score = 28.5 bits (62), Expect = 3.7
Identities = 13/29 (44%), Positives = 15/29 (50%)
Frame = -3
Query: 247 SREFTCFCKDGSFPHSCSSGEHHCRFHCC 275
S+ T C GSF +S SS H C F C
Sbjct: 202 SKRVTLTCCFGSFSNSSSSSTHSCFFFSC 116
>TC220174
Length = 1008
Score = 28.1 bits (61), Expect = 4.9
Identities = 33/120 (27%), Positives = 52/120 (42%), Gaps = 8/120 (6%)
Frame = +2
Query: 171 ADNAESLDS---FSETLR--TCKSRVVVPF---LESEAERVATNSSVENLKDVIDNGFGV 222
A N E +D SE L+ TC + VVPF L + + N + NL + D F +
Sbjct: 110 AGNNEEMDGELEISEDLKKSTC-THPVVPFQNGLHTACDPETQNVEIGNLITISDQPFSL 286
Query: 223 EWNANNSLCHECQSSGGHCGYESSSREFTCFCKDGSFPHSCSSGEHHCRFHCCVMFLHSI 282
+++ +SS G + ++ + +GS P SG C + CC LHS+
Sbjct: 287 -----SAVETATKSSDGKSDKQENATDNNVSSSNGSGP--AESGVI-CLYQCCPACLHSL 442
>AW277953 similar to GP|10441016|gb alpha-tubulin {Oryza sativa}, partial
(31%)
Length = 481
Score = 27.7 bits (60), Expect = 6.4
Identities = 13/42 (30%), Positives = 16/42 (37%), Gaps = 5/42 (11%)
Frame = -3
Query: 239 GHCGYESSSREFTCFCKD-----GSFPHSCSSGEHHCRFHCC 275
G C S C C+ PH C+ E+ R HCC
Sbjct: 236 GWCRLASQCPVNACICQKRLHCRAGQPHQCAQEENEWRAHCC 111
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.133 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,971,430
Number of Sequences: 63676
Number of extensions: 355128
Number of successful extensions: 2175
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 2152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2168
length of query: 293
length of database: 12,639,632
effective HSP length: 97
effective length of query: 196
effective length of database: 6,463,060
effective search space: 1266759760
effective search space used: 1266759760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147875.3