BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147714.14 + phase: 0
(129 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC232995 74 1e-14
TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, co... 72 5e-14
TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete 71 1e-13
TC211311 weakly similar to UP|O24587 (O24587) Pol protein, parti... 64 1e-11
BI321712 60 2e-10
TC232593 weakly similar to UP|Q9XG91 (Q9XG91) Tpv2-1c protein (F... 59 5e-10
CO983516 54 2e-08
AI855818 weakly similar to GP|21741393|e OSJNBb0051N19.6 {Oryza ... 52 6e-08
BM143109 52 6e-08
AI959950 47 2e-06
BI972503 weakly similar to GP|18378607|gb polyprotein {Oryza sat... 45 9e-06
BI787454 weakly similar to GP|21434|emb|CA ORF4 {Solanum tuberos... 43 4e-05
CA853145 weakly similar to GP|27901709|gb| gag-pol polyprotein {... 43 4e-05
BI469652 weakly similar to GP|18149115|dbj| reverse transcriptas... 42 6e-05
BE211208 39 5e-04
BF595216 39 7e-04
BF596070 similar to GP|27901698|gb gag-pol polyprotein {Vitis vi... 36 0.006
BM307983 34 0.017
BQ081067 weakly similar to GP|23495377|dbj orf490 {Oryza sativa ... 33 0.048
BI317550 weakly similar to GP|9759590|dbj| polyprotein-like {Ara... 32 0.063
>TC232995
Length = 1009
Score = 74.3 bits (181), Expect = 1e-14
Identities = 33/78 (42%), Positives = 52/78 (66%)
Frame = +2
Query: 35 KDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTL 94
+ E EMSMMGELK+FL +QI Q + ++++QSKY K+L+ +F +D M+TPM C L
Sbjct: 275 QSEFEMSMMGELKYFLGLQIKQTQ*GIFINQSKYCKELIKRFGMDSAKHMSTPMSTNCYL 454
Query: 95 SEEDADAKVDQKLFRGII 112
++++ +D K +R I
Sbjct: 455 DKDESGQSIDIKQYRDAI 508
Score = 27.7 bits (60), Expect = 1.5
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 18 VKQPPGFEDLKHPDYVYK 35
V+QPPGFE P++VYK
Sbjct: 2 VEQPPGFEISDKPNHVYK 55
>TC204438 homologue to UP|Q84VH6 (Q84VH6) Gag-pol polyprotein, complete
Length = 4734
Score = 72.4 bits (176), Expect = 5e-14
Identities = 36/97 (37%), Positives = 61/97 (62%)
Frame = +1
Query: 25 EDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEM 84
E L+H + E EMS++GEL +FL +Q+ Q +D +++ QSKY K+++ KF +++ +
Sbjct: 3820 EMLRHFVQQMQSEFEMSLVGELTYFLGLQVKQMEDSIFLSQSKYAKNIVKKFGMENASHK 3999
Query: 85 NTPMHPTCTLSEEDADAKVDQKLFRGIILFDDSILFL 121
TP LS+++A VDQ L+R +I S+L+L
Sbjct: 4000 RTPAPTHLKLSKDEAGTSVDQSLYRSMI---GSLLYL 4101
Score = 52.8 bits (125), Expect = 4e-08
Identities = 23/35 (65%), Positives = 28/35 (79%)
Frame = +1
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M VKSAFLNG ++EE YV+QP GF D HPD+VY+
Sbjct: 3526 MDVKSAFLNGYLNEEAYVEQPKGFVDPTHPDHVYR 3630
>TC204439 UP|Q84VI4 (Q84VI4) Gag-pol polyprotein, complete
Length = 4731
Score = 71.2 bits (173), Expect = 1e-13
Identities = 35/97 (36%), Positives = 61/97 (62%)
Frame = +1
Query: 25 EDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEM 84
E L+H + E EMS++GEL +FL +Q+ Q +D +++ QS+Y K+++ KF +++ +
Sbjct: 3817 EMLRHFVQQMQSEFEMSLVGELTYFLGLQVKQMEDSIFLSQSRYAKNIVKKFGMENASHK 3996
Query: 85 NTPMHPTCTLSEEDADAKVDQKLFRGIILFDDSILFL 121
TP LS+++A VDQ L+R +I S+L+L
Sbjct: 3997 RTPAPTHLKLSKDEAGTSVDQSLYRSMI---GSLLYL 4098
Score = 54.7 bits (130), Expect = 1e-08
Identities = 24/35 (68%), Positives = 29/35 (82%)
Frame = +1
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M VKSAFLNG ++EEVYV+QP GF D HPD+VY+
Sbjct: 3523 MDVKSAFLNGYLNEEVYVEQPKGFADPTHPDHVYR 3627
>TC211311 weakly similar to UP|O24587 (O24587) Pol protein, partial (15%)
Length = 1213
Score = 64.3 bits (155), Expect = 1e-11
Identities = 34/87 (39%), Positives = 51/87 (58%)
Frame = +3
Query: 35 KDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTL 94
KD E SM GELKF L +QI Q +++HQ KYTK L +F++D+ M TPMH + +
Sbjct: 570 KDGFETSMKGELKFLLGLQIIQKVYGIFIHQEKYTKSHLKRFRMDEAKPMATPMHRSTII 749
Query: 95 SEEDADAKVDQKLFRGIILFDDSILFL 121
+++ K + G+I DS+ +L
Sbjct: 750 DKDEKGNHTS*KEYSGMI---DSLSYL 821
>BI321712
Length = 399
Score = 60.5 bits (145), Expect = 2e-10
Identities = 31/92 (33%), Positives = 51/92 (54%)
Frame = -3
Query: 21 PPGFEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDD 80
P FE+ K +E EM+ MG + ++L I++ Q +++ Q Y K++L KFK+DD
Sbjct: 343 PSMFEEFKKD---MSNEFEMTDMGLMAYYLGIEVKQEDKGIFITQEGYAKEVLKKFKMDD 173
Query: 81 CNEMNTPMHPTCTLSEEDADAKVDQKLFRGII 112
N + TPM LS+ + VD L++ +I
Sbjct: 172 ANPVGTPMECGSKLSKHEKGENVDPTLYKSLI 77
>TC232593 weakly similar to UP|Q9XG91 (Q9XG91) Tpv2-1c protein (Fragment),
partial (16%)
Length = 562
Score = 59.3 bits (142), Expect = 5e-10
Identities = 25/74 (33%), Positives = 49/74 (65%)
Frame = +1
Query: 39 EMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEED 98
EM+ +G + +FL I+I Q +++V + Q KY K++L KF++++C ++TPM+ ++ D
Sbjct: 286 EMTNLGLMTYFLGIEIKQSQNKVLICQRKYAKEILKKFQMEECKSVSTPMNQKEKFNKVD 465
Query: 99 ADAKVDQKLFRGII 112
K+D+ +R +I
Sbjct: 466 GADKIDEGYYRSLI 507
>CO983516
Length = 724
Score = 53.9 bits (128), Expect = 2e-08
Identities = 24/35 (68%), Positives = 29/35 (82%)
Frame = +2
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M VKSAFLNG ++EEVYV+QP GF D HPD+VY+
Sbjct: 443 MDVKSAFLNGYLNEEVYVEQPKGFIDPTHPDHVYR 547
>AI855818 weakly similar to GP|21741393|e OSJNBb0051N19.6 {Oryza sativa
(japonica cultivar-group)}, partial (10%)
Length = 463
Score = 52.4 bits (124), Expect = 6e-08
Identities = 25/35 (71%), Positives = 27/35 (76%)
Frame = -3
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M VKSAFLNG I EEVYV+QPPGFE P +VYK
Sbjct: 182 MDVKSAFLNGLIQEEVYVEQPPGFEIPDKPTHVYK 78
>BM143109
Length = 415
Score = 52.4 bits (124), Expect = 6e-08
Identities = 23/46 (50%), Positives = 35/46 (76%)
Frame = +1
Query: 35 KDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDD 80
++E EMSMM EL FFL +QI Q K+ +++ QSKY KDL+ +F +++
Sbjct: 271 QNEFEMSMMRELNFFLGLQIKQTKNGIFISQSKYCKDLIHRFGMEN 408
>AI959950
Length = 466
Score = 47.0 bits (110), Expect = 2e-06
Identities = 22/35 (62%), Positives = 27/35 (76%)
Frame = -1
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M VKSAFLNG I +EVYV+QPPGFE+ +V+K
Sbjct: 115 MDVKSAFLNGLIQKEVYVEQPPGFENETLHQHVFK 11
>BI972503 weakly similar to GP|18378607|gb polyprotein {Oryza sativa
(japonica cultivar-group)}, partial (5%)
Length = 327
Score = 45.1 bits (105), Expect = 9e-06
Identities = 20/70 (28%), Positives = 40/70 (56%)
Frame = +2
Query: 43 MGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEEDADAK 102
+G LK+FL I++ Q +V + Q KY D+L + + +C +++PM P L + ++
Sbjct: 71 LGYLKYFLGIEVAQSGVDVVISQRKYALDILEETGMQNCRPVDSPMDPNLKLMADQSEIY 250
Query: 103 VDQKLFRGII 112
D + +R ++
Sbjct: 251 HDPERYRRLV 280
>BI787454 weakly similar to GP|21434|emb|CA ORF4 {Solanum tuberosum}, partial
(21%)
Length = 421
Score = 43.1 bits (100), Expect = 4e-05
Identities = 20/67 (29%), Positives = 37/67 (54%)
Frame = +2
Query: 46 LKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEEDADAKVDQ 105
LK+FL I++ Q D V + Q KY D+L + + +C +++PM P L ++ D
Sbjct: 173 LKYFLGIEVAQSGDGVVISQRKYALDILEETGMQNCRLVDSPMDPNLKLMAYQSEVYPDP 352
Query: 106 KLFRGII 112
+ +R ++
Sbjct: 353 ERYRRLV 373
>CA853145 weakly similar to GP|27901709|gb| gag-pol polyprotein {Vitis
vinifera}, partial (29%)
Length = 563
Score = 43.1 bits (100), Expect = 4e-05
Identities = 22/68 (32%), Positives = 37/68 (54%)
Frame = +3
Query: 23 GFEDLKHPDYVYKDEIEMSMMGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCN 82
G DLK + + + +G+LK+FL I++ Q K + + Q KY D+L + +C
Sbjct: 36 GIRDLKSHLFQH---FQTKDLGQLKYFLGIEVAQSKTGIAICQRKYALDILKDTGMQNCK 206
Query: 83 EMNTPMHP 90
++TPM P
Sbjct: 207 PVDTPMDP 230
>BI469652 weakly similar to GP|18149115|dbj| reverse transcriptase {Silene
noctiflora}, partial (60%)
Length = 427
Score = 42.4 bits (98), Expect = 6e-05
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = +3
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFEDLKHPDYVYK 35
M +KS FLN I EEVYV+QPP F D D+++K
Sbjct: 258 MDIKSGFLNDFIEEEVYVRQPPNFVDHTLLDHIFK 362
>BE211208
Length = 413
Score = 39.3 bits (90), Expect = 5e-04
Identities = 21/74 (28%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
Frame = +2
Query: 40 MSMMGELKFFLRIQINQCKD-EVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTLSEED 98
+ +G+L +FL I+++ V + QSKY DLL K + + +++PM LS+
Sbjct: 122 LKQLGQLDYFLGIEVHHTPTGSVLLTQSKYICDLLHKTDMAEAKPISSPMVTNLRLSKNG 301
Query: 99 ADAKVDQKLFRGII 112
D D ++R ++
Sbjct: 302 DDLLSDPTMYRSVV 343
>BF595216
Length = 421
Score = 38.9 bits (89), Expect = 7e-04
Identities = 15/25 (60%), Positives = 21/25 (84%)
Frame = +3
Query: 1 MKVKSAFLNGAISEEVYVKQPPGFE 25
+ V +AFLNG + EEVY++QPPGF+
Sbjct: 300 LDVNNAFLNGILQEEVYMQQPPGFD 374
>BF596070 similar to GP|27901698|gb gag-pol polyprotein {Vitis vinifera},
partial (34%)
Length = 407
Score = 35.8 bits (81), Expect = 0.006
Identities = 12/24 (50%), Positives = 21/24 (87%)
Frame = -2
Query: 1 MKVKSAFLNGAISEEVYVKQPPGF 24
+ +K+AFL+G + E++Y++QPPGF
Sbjct: 181 LDIKNAFLHGDLEEDIYMEQPPGF 110
>BM307983
Length = 406
Score = 34.3 bits (77), Expect = 0.017
Identities = 13/22 (59%), Positives = 20/22 (90%)
Frame = +2
Query: 3 VKSAFLNGAISEEVYVKQPPGF 24
VK+AFL+G++ EEVY++ PPG+
Sbjct: 209 VKNAFLHGSLEEEVYMEIPPGY 274
>BQ081067 weakly similar to GP|23495377|dbj orf490 {Oryza sativa (japonica
cultivar-group)}, partial (18%)
Length = 430
Score = 32.7 bits (73), Expect = 0.048
Identities = 13/23 (56%), Positives = 19/23 (82%)
Frame = +3
Query: 3 VKSAFLNGAISEEVYVKQPPGFE 25
+ +AFL+G ++EEVY+ Q PGFE
Sbjct: 360 INNAFLHGLLTEEVYMVQLPGFE 428
>BI317550 weakly similar to GP|9759590|dbj| polyprotein-like {Arabidopsis
thaliana}, partial (18%)
Length = 421
Score = 32.3 bits (72), Expect = 0.063
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = -2
Query: 43 MGELKFFLRIQINQCKDEVYVHQSKYTKDLLMKFKVDDCNEMNTPMHPTCTL 94
+G+LK+FL +++ + + + Q KY DLL + C +TP+ + L
Sbjct: 183 LGKLKYFLGLEVAHSRLGITISQRKYCLDLLKDSGLLGCKPASTPLDTSIKL 28
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.323 0.141 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,284,855
Number of Sequences: 63676
Number of extensions: 63719
Number of successful extensions: 259
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 256
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of query: 129
length of database: 12,639,632
effective HSP length: 86
effective length of query: 43
effective length of database: 7,163,496
effective search space: 308030328
effective search space used: 308030328
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC147714.14