BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147536.4 + phase: 0 /pseudo
(805 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211561 weakly similar to UP|O22675 (O22675) Reverse transcript... 120 3e-27
CD417230 weakly similar to GP|2244915|emb| reverse transcriptase... 115 6e-26
TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR ret... 53 2e-14
CO982349 77 2e-14
AW757033 75 1e-13
TC212765 69 8e-12
BI315739 59 6e-09
BI471325 43 5e-04
BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase ... 42 0.001
TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance... 42 0.001
BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis ... 32 0.85
BI972659 31 1.9
TC206249 30 3.2
>TC211561 weakly similar to UP|O22675 (O22675) Reverse transcriptase
(Fragment) , partial (59%)
Length = 1077
Score = 120 bits (300), Expect = 3e-27
Identities = 72/215 (33%), Positives = 110/215 (50%)
Frame = +1
Query: 5 KGNKAFMTIQIDLEKAYDRLNWNFVEECLMECKFPSKLIKIIHHCISTLSYKIMWNGDKT 64
+ NK+ + ++D EKAYD ++WNF+ + F K IK I C+ + S ++ NG T
Sbjct: 46 RSNKSCLVFKVDYEKAYDSVSWNFLMYMMWRMDFSPKWIKWIEECVKSASISVLVNGSPT 225
Query: 65 ETFYPSRGIRQGDPLSPYLFVICMERLSHIIADQVESDYWKPMRVGRYGPLISHLLFADD 124
F P RG+RQGDPL+P+LF I E L+ ++ VE + +KP VG G IS L +ADD
Sbjct: 226 AEFLPQRGLRQGDPLTPFLFNIVAEGLNGLMRRAVEENLYKPYLVGANGVPISILQYADD 405
Query: 125 LLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVYFSKNVDNHLREDIIQHTGFNQV 184
+ F EA+ E + L F S +IN K+ + V + +++ + + +
Sbjct: 406 TIFFGEAAKENVEAIKVILRSFELVSNLRINFAKS-CFGVFGVTDQWKQEAANYLHCSLL 582
Query: 185 NNLGKYLGANIAPGRTSRGHFNHIINKIQNKLSGW 219
YLG I ++ II K + KLS W
Sbjct: 583 AFPFIYLGIPIGANSRRCHMWDSIIRKCERKLSKW 687
>CD417230 weakly similar to GP|2244915|emb| reverse transcriptase like
protein {Arabidopsis thaliana}, partial (7%)
Length = 688
Score = 115 bits (289), Expect = 6e-26
Identities = 55/132 (41%), Positives = 90/132 (67%), Gaps = 1/132 (0%)
Frame = -3
Query: 43 IKIIHHCISTLSYKIMWNGDKTETFYPSRGIRQGDPLSPYLFVICMERLSHIIADQVESD 102
++++ +C+ST + ++WNG+ + F P+ G+RQ DP++PYLFV+C+ERLSH+I +
Sbjct: 686 VQLVWYCMSTTTMSLLWNGEVLDHFSPNIGVRQRDPIAPYLFVLCLERLSHLINLVMNKK 507
Query: 103 YWKPMRVGRYGPLISHLLFADDLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVY 162
+ ++V R G LISHL F DDL+LF EA+++Q + L++F SSG K+N +KT+ +
Sbjct: 506 L*RSIQVNRGGLLISHLAFVDDLILFVEANMDQVDVINLTLELFSDSSGAKVNVDKTKSF 327
Query: 163 -FSKNVDNHLRE 173
F K + LR+
Sbjct: 326 LFPKMLIGGLRK 291
Score = 43.5 bits (101), Expect = 4e-04
Identities = 23/61 (37%), Positives = 32/61 (51%)
Frame = -2
Query: 163 FSKNVDNHLREDIIQHTGFNQVNNLGKYLGANIAPGRTSRGHFNHIINKIQNKLSGWMFK 222
FSKNV+ + EDI G ++LGKYLG I + F+ II+K+ + S W
Sbjct: 324 FSKNVNWWVEEDISSGLGLQSTDDLGKYLGVPIFHKNPTSHTFDFIIDKVNKRFSRWKSN 145
Query: 223 L 223
L
Sbjct: 144 L 142
>TC233727 similar to UP|Q9FKH8 (Q9FKH8) Similarity to non-LTR retroelement
reverse transcriptase, partial (7%)
Length = 956
Score = 52.8 bits (125), Expect(2) = 2e-14
Identities = 28/105 (26%), Positives = 56/105 (52%), Gaps = 2/105 (1%)
Frame = -2
Query: 117 SHLLFADDLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVYFSKNVDNHLR--ED 174
SH++ ADD+++F + + H +++ ++ S GQ I++ K++++ LR D
Sbjct: 451 SHVMHADDIMIFCKGTSRNVHNLINFFQLYKDSFGQVISKHKSKIFIGSIPGQRLRVISD 272
Query: 175 IIQHTGFNQVNNLGKYLGANIAPGRTSRGHFNHIINKIQNKLSGW 219
++Q+ + N Y G I G+ +R H + +KI+ KL+ W
Sbjct: 271 LLQYFHSDIPFN---YFGVPIFKGKPTRRHLYCVADKIKCKLASW 146
Score = 45.1 bits (105), Expect(2) = 2e-14
Identities = 24/74 (32%), Positives = 39/74 (52%)
Frame = -3
Query: 11 MTIQIDLEKAYDRLNWNFVEECLMECKFPSKLIKIIHHCISTLSYKIMWNGDKTETFYPS 70
+ ++ID+ KA+D L+ +F+ L + + I + + I NG+ F
Sbjct: 765 LALKIDIVKAFDTLDRDFLISVLNKFGYSHTFCNWIRVILLSAKLSISVNGEPVGFFSCQ 586
Query: 71 RGIRQGDPLSPYLF 84
RG+RQGDPLSP L+
Sbjct: 585 RGVRQGDPLSPSLY 544
>CO982349
Length = 795
Score = 77.4 bits (189), Expect = 2e-14
Identities = 53/174 (30%), Positives = 84/174 (47%)
Frame = +3
Query: 46 IHHCISTLSYKIMWNGDKTETFYPSRGIRQGDPLSPYLFVICMERLSHIIADQVESDYWK 105
I C+ + S I+ N F P RG+RQGDPL+P LF I E L+ ++ + V+ +
Sbjct: 72 IRGCLHSASVSILVNESPINEFSPQRGLRQGDPLAPLLFNIVAEGLTGLMREAVDRKRFN 251
Query: 106 PMRVGRYGPLISHLLFADDLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVYFSK 165
VG+ +S L +ADD + F EA++E + L F +SG KIN +++
Sbjct: 252 SFLVGKNKEPVSILQYADDTIFFEEATMENMKVIKTILRCFELASGLKINFAESRFGAIW 431
Query: 166 NVDNHLREDIIQHTGFNQVNNLGKYLGANIAPGRTSRGHFNHIINKIQNKLSGW 219
D H ++ + + ++ YLG I R + II K + KL+ W
Sbjct: 432 KPD-HWCKEAAEFLNCSMLSMPFSYLGIPIEANPRCREI*DPIIRKCETKLARW 590
>AW757033
Length = 441
Score = 75.1 bits (183), Expect = 1e-13
Identities = 48/146 (32%), Positives = 79/146 (53%)
Frame = -2
Query: 64 TETFYPSRGIRQGDPLSPYLFVICMERLSHIIADQVESDYWKPMRVGRYGPLISHLLFAD 123
T F P RG+RQGDPL+P LF I E L+ ++ + V +++K VG+Y +S L +AD
Sbjct: 440 TREFSPHRGLRQGDPLAPLLFNIAAEGLTGLMREAVARNHYKSFAVGKYKKPVSILQYAD 261
Query: 124 DLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVYFSKNVDNHLREDIIQHTGFNQ 183
D + F EA++E + L F +SG KIN K++ + + +V + R++ + +
Sbjct: 260 DTIFFGEATMENVRVIKTILRGFELASGLKINFAKSR-FGAISVPD*WRKEAAEFMNCSL 84
Query: 184 VNNLGKYLGANIAPGRTSRGHFNHII 209
++ YLG I R ++ II
Sbjct: 83 LSLPFSYLGIPIGANPRRRETWDPII 6
>TC212765
Length = 637
Score = 68.9 bits (167), Expect = 8e-12
Identities = 45/134 (33%), Positives = 67/134 (49%)
Frame = +3
Query: 81 PYLFVICMERLSHIIADQVESDYWKPMRVGRYGPLISHLLFADDLLLFAEASIEQAHCVL 140
PYLFV+CMERL+ I D W+P+ V L+ +L+ DD+ LF A + A+
Sbjct: 15 PYLFVLCMERLALCIHDLTSQ*IWRPILVSDDNRLVPYLMLVDDIFLFINAIGD*AYLAS 194
Query: 141 HCLDMFCQSSGQKINREKTQVYFSKNVDNHLREDIIQHTGFNQVNNLGKYLGANIAPGRT 200
L F Q+ G K+N +K+ +Y S + LR+ I +LGKYL GR+
Sbjct: 195 LTL*NFFQTFGLKLNLDKSSMYCS*RMAPVLRDLISNTLNIVHAPSLGKYLCLKFIHGRS 374
Query: 201 SRGHFNHIINKIQN 214
F ++ +I N
Sbjct: 375 KIVDF-LLVERIHN 413
>BI315739
Length = 442
Score = 59.3 bits (142), Expect = 6e-09
Identities = 44/145 (30%), Positives = 71/145 (48%)
Frame = +3
Query: 78 PLSPYLFVICMERLSHIIADQVESDYWKPMRVGRYGPLISHLLFADDLLLFAEASIEQAH 137
PLSPYLF ICME+L+ +I +V + W+P+++ R GP IS + + ++ F +
Sbjct: 48 PLSPYLF*ICMEKLAILIQSKVNEETWEPIKISRNGPGISPIYSSQIIVYFL*KPLV--- 218
Query: 138 CVLHCLDMFCQSSGQKINREKTQVYFSKNVDNHLREDIIQHTGFNQVNNLGKYLGANIAP 197
L+ +M C S + E ++ F H R +I+ L L +
Sbjct: 219 LKLNW*EMCCNSF---V*PEVSRRVFKNPGF*HKRMCLIE--------KLPGSLL**LLA 365
Query: 198 GRTSRGHFNHIINKIQNKLSGWMFK 222
GRT + F H+++ I N+L GW K
Sbjct: 366 GRTKKEDFAHVVDMINNRLDGWKSK 440
>BI471325
Length = 421
Score = 43.1 bits (100), Expect = 5e-04
Identities = 24/76 (31%), Positives = 39/76 (50%)
Frame = +2
Query: 107 MRVGRYGPLISHLLFADDLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQVYFSKN 166
++V R ++SHLL D LF ++A + + L F SSG +N K+++ FS+N
Sbjct: 17 VKVHRGITILSHLLSVDFYFLFCRTIDKEADVLKNILTTFEASSGLVVNLRKSEICFSRN 196
Query: 167 VDNHLREDIIQHTGFN 182
R+ I H G +
Sbjct: 197 TSQIARDSIFFHLGMS 244
>BI470652 similar to PIR|T17102|T171 RNA-directed DNA polymerase (EC
2.7.7.49) (clone AmLi2) - garden snapdragon (fragment),
partial (22%)
Length = 111
Score = 42.0 bits (97), Expect = 0.001
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = +1
Query: 49 CISTLSYKIMWNGDKTETFYPSRGIRQGDPLSPY 82
C+S+ S I+ NG TE F P RG+RQG P P+
Sbjct: 7 CLSSASISILVNGSPTEEFKPXRGLRQGGPFGPF 108
>TC233219 weakly similar to UP|Q8LKI9 (Q8LKI9) NBS/LRR resistance
protein-like protein (Fragment), partial (74%)
Length = 781
Score = 42.0 bits (97), Expect = 0.001
Identities = 45/177 (25%), Positives = 79/177 (44%), Gaps = 10/177 (5%)
Frame = +3
Query: 43 IKIIHHCISTLSYKIMWNGDKTETFYPSRGIRQGDPLSPYLFVICMERLSHIIADQVESD 102
I+ I +S + G +++ F + G+ QG LSPYLF + ++ ++ +Q++
Sbjct: 45 IRAIQDMYDRVSTSVRTQGGESDDFPITIGLHQGSTLSPYLFTLILD----VLTEQIQE- 209
Query: 103 YWKPMRVGRYGPLISH-LLFADDLLLFAEASIEQAHCVLHCLDMFCQSSGQKINREKTQV 161
++S +LFADD++L E S E+ + L ++ G +++R K++
Sbjct: 210 ------------IVSRCMLFADDIVLLGE-SREKLNERLETWRRALETHGFRLSRSKSEY 350
Query: 162 YFSK--------NVDNHLREDII-QHTGFNQVNNLGKYLGANIAPGRTSRGHFNHII 209
K N + + + II Q T F KYLG+ I G NH I
Sbjct: 351 MECKFNKRRRVSNSEVKIGDHIIPQVTRF-------KYLGSVIQDDGEIEGDVNHRI 500
>BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis thaliana},
partial (1%)
Length = 422
Score = 32.3 bits (72), Expect = 0.85
Identities = 19/46 (41%), Positives = 24/46 (51%)
Frame = +2
Query: 273 SSFGELGCLLSS*DA*WSWF*AASSYE*GIPYEDVMESYQAIR*AL 318
SS EL L+ *+ WSWF SYE PYE +E +*+L
Sbjct: 5 SSLDELESLMLP*EKWWSWFAIVESYEQISPYEVYLEHVYKTK*SL 142
>BI972659
Length = 453
Score = 31.2 bits (69), Expect = 1.9
Identities = 31/93 (33%), Positives = 41/93 (43%), Gaps = 3/93 (3%)
Frame = +1
Query: 130 EASIEQAHCVLHCLDMFCQSSGQKINREKTQ---VYFSKNVDNHLREDIIQHTGFNQVNN 186
EAS + + L F SG +IN K+Q V F N D Q Q++
Sbjct: 7 EASWDNIIVLKSMLRGFEMVSGLRINYAKSQFGIVGFQPN----WAHDAAQLLNCRQLDI 174
Query: 187 LGKYLGANIAPGRTSRGHFNHIINKIQNKLSGW 219
YLG IA +SR + +INK + KLS W
Sbjct: 175 PFHYLGMPIAVKASSRMVWEPLINKFKAKLSKW 273
>TC206249
Length = 837
Score = 30.4 bits (67), Expect = 3.2
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2
Query: 150 SGQKINREKTQVYFSKNVDNHLREDIIQHTGFN 182
SGQ+ N + Q Y N +H++E +QHT F+
Sbjct: 266 SGQETNGIRNQQY*QNNCKSHVQEHGVQHTSFS 364
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.363 0.164 0.635
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,128,447
Number of Sequences: 63676
Number of extensions: 650736
Number of successful extensions: 6270
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 3392
Number of HSP's successfully gapped in prelim test: 222
Number of HSP's that attempted gapping in prelim test: 2795
Number of HSP's gapped (non-prelim): 3774
length of query: 805
length of database: 12,639,632
effective HSP length: 105
effective length of query: 700
effective length of database: 5,953,652
effective search space: 4167556400
effective search space used: 4167556400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (22.0 bits)
S2: 63 (28.9 bits)
Medicago: description of AC147536.4