BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147496.3 - phase: 0
(294 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG239747 123 1e-28
TC228120 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%) 69 2e-12
TC229090 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%) 60 9e-10
NP595167 R 13 protein [Glycine max] 50 9e-07
NP595166 R 14 protein [Glycine max] 50 9e-07
TC228121 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (9%) 35 0.031
TC233520 34 0.068
TC220327 similar to UP|Q8RY07 (Q8RY07) Serine/threonine protein ... 29 2.2
TC227282 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arab... 29 2.9
TC206971 homologue to UP|RL35_EUPES (Q9M5L0) 60S ribosomal prote... 29 2.9
TC213102 homologue to UP|O82155 (O82155) Dof zinc finger protein... 28 4.9
TC215984 similar to UP|Q9FMP3 (Q9FMP3) Dihydropyrimidinase (Dihy... 28 4.9
TC220860 28 6.4
BE023716 weakly similar to GP|9758876|dbj disease resistance pro... 28 6.4
TC230207 similar to UP|Q9LTR6 (Q9LTR6) Gb|AAF36750.1, partial (28%) 27 8.4
CO984054 27 8.4
TC231346 weakly similar to UP|Q6QM04 (Q6QM04) LRR protein WM1.2,... 27 8.4
CD418668 27 8.4
>BG239747
Length = 360
Score = 123 bits (308), Expect = 1e-28
Identities = 60/87 (68%), Positives = 72/87 (81%)
Frame = +3
Query: 32 NSKFFIPNNNNIIHRILLILFVAIISIWANYEASKTFDIHIVNDTKDSLAGRRFTLFYVS 91
N+K F ++NNI+ RIL IL +A+IS+WANYEASKTF I +VND KDS AGRRF L YVS
Sbjct: 84 NAKHFTSDSNNIVLRILSILLLALISLWANYEASKTFHITVVNDAKDSPAGRRFALSYVS 263
Query: 92 NDKASRILLNTSSFVEQILYPNGNNDN 118
ND+ASRILLNTSS+VE LYPN NN++
Sbjct: 264 NDEASRILLNTSSYVEHFLYPNSNNND 344
>TC228120 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%)
Length = 1060
Score = 69.3 bits (168), Expect = 2e-12
Identities = 57/241 (23%), Positives = 105/241 (42%), Gaps = 2/241 (0%)
Frame = +3
Query: 55 IISIWANYEASKTFDIHIVNDTKDSLAGRRFTLFYVSNDKASRILLNTSSFVEQILYPNG 114
++ + A + + D + N+ + G RF + +D A + L + + F+ +L N
Sbjct: 75 LVFLAAAMQGTLAVDYTVTNNALSTSGGVRFR-DAIGDDYAKQTLDSATQFIWGVLQQNA 251
Query: 115 NNDNIDIKNKKIIKSVTLRLARQNLDTITTITADEKNINS-YVIEISPMLLEDENFNNMA 173
D D++ + L ++D + + DE ++++ YV + S L +
Sbjct: 252 PADRKDVQK--------VNLFVDDMDGVAYTSNDEIHLSARYVNDYSGDLKTE------- 386
Query: 174 IVGAIQRAMARVWLWDGRSKASPRLLDGMVEYIAELAGFHRERLSGGVGESPECEDGR-E 232
I G + M VW W+G + L++G+ +Y+ RL S + G+ E
Sbjct: 387 ITGVLYHEMVHVWQWNGNGQTPSGLIEGIADYV---------RLKANYAPSHWVKAGQGE 539
Query: 233 LWWDNKDPTHVARLLHYCEKYDKGFIQRLNEAMKDTWHDRMVGDVLGLKATKLCGLYNAT 292
W D T A L YC+ GF+ +LN+ M+D + D++ +LG +L Y A
Sbjct: 540 KWDQGYDVT--AHFLDYCDSLKSGFVAQLNQQMRDGYSDQLFVQLLGKTVDQLWQDYKAK 713
Query: 293 Y 293
Y
Sbjct: 714 Y 716
>TC229090 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (31%)
Length = 882
Score = 60.5 bits (145), Expect = 9e-10
Identities = 54/255 (21%), Positives = 109/255 (42%)
Frame = +2
Query: 39 NNNNIIHRILLILFVAIISIWANYEASKTFDIHIVNDTKDSLAGRRFTLFYVSNDKASRI 98
N I H + ++F++ + A+ + + N+ + G F + + A R
Sbjct: 26 NTTMINHALCFLVFLSAL------HATHGVEYTVTNNALSTPGGVAFR-DKIGAEYAKRT 184
Query: 99 LLNTSSFVEQILYPNGNNDNIDIKNKKIIKSVTLRLARQNLDTITTITADEKNINSYVIE 158
L + + F+ +I N +D +K ++ V+L + ++D + + ++ ++++ +
Sbjct: 185 LDSATQFIWRIFQQNNPSD------RKNVQKVSLFV--DDMDGVAYTSNNQIHLSARYVG 340
Query: 159 ISPMLLEDENFNNMAIVGAIQRAMARVWLWDGRSKASPRLLDGMVEYIAELAGFHRERLS 218
+ ++ E I G + M VW W G +A L++G+ +Y+ RL
Sbjct: 341 NNKGDVKTE------ITGVLYHEMVHVWQWSGNGRAPGGLIEGIADYV---------RLK 475
Query: 219 GGVGESPECEDGRELWWDNKDPTHVARLLHYCEKYDKGFIQRLNEAMKDTWHDRMVGDVL 278
S + G+ WD AR L YC+ GF+ +LN+ M+ + D+ +L
Sbjct: 476 ANYAPSHWVKAGQGQKWDQGYDV-TARFLDYCDSLKSGFVAQLNKLMRTGYSDQYFVQLL 652
Query: 279 GLKATKLCGLYNATY 293
G +L Y A Y
Sbjct: 653 GKPVDQLWRDYKAKY 697
>NP595167 R 13 protein [Glycine max]
Length = 1926
Score = 50.4 bits (119), Expect = 9e-07
Identities = 52/223 (23%), Positives = 95/223 (42%), Gaps = 1/223 (0%)
Frame = +1
Query: 72 IVNDTKDSLAGRRFTLFYVSNDKASRILLNTSSFVEQILYPNGNNDNIDIKNKKIIKSVT 131
++N+ +L G RF + + A++ L + + F+ +I N +D +K ++ V+
Sbjct: 1318 VINNALTTLGGVRFR-DKIGAEYANQTLDSATQFIWEIFQQNNPSD------RKSVQKVS 1476
Query: 132 LRLARQNLDTITTITADEKNINS-YVIEISPMLLEDENFNNMAIVGAIQRAMARVWLWDG 190
L + ++D I +E ++++ YV S ++ E I G + + VW W G
Sbjct: 1477 LFV--DDMDGIAYTRKNEIHVSARYVNGYSGGDVKRE------ITGVLFHQVCYVWQWKG 1632
Query: 191 RSKASPRLLDGMVEYIAELAGFHRERLSGGVGESPECEDGRELWWDNKDPTHVARLLHYC 250
KA L G+ +++ RL S + G+ W N+ A L YC
Sbjct: 1633 NGKAPGGLTGGIADFV---------RLKANYAASHWRKPGQGQKW-NEGYEITAHFLDYC 1782
Query: 251 EKYDKGFIQRLNEAMKDTWHDRMVGDVLGLKATKLCGLYNATY 293
+ GF+ +LN+ M+ + D +L L Y A Y
Sbjct: 1783 DSLKSGFVGQLNQWMRTDYSDEFFFLLLAKPVNHLWRDYKAMY 1911
>NP595166 R 14 protein [Glycine max]
Length = 1926
Score = 50.4 bits (119), Expect = 9e-07
Identities = 54/245 (22%), Positives = 105/245 (42%), Gaps = 1/245 (0%)
Frame = +1
Query: 50 ILFVAIISIWANYEASKTFDIHIVNDTKDSLAGRRFTLFYVSNDKASRILLNTSSFVEQI 109
I+ + +S A + ++ + ++N+ + G RF + + A+R L + FV +I
Sbjct: 1252 IVDILKVSFDATKQETQGYKFTVINNALTTPGGVRFR-DKIGAEYANRTLELATQFVWRI 1428
Query: 110 LYPNGNNDNIDIKNKKIIKSVTLRLARQNLDTITTITADEKNINS-YVIEISPMLLEDEN 168
+D +K ++ V+L + +++ I +E ++++ YV S ++ E
Sbjct: 1429 FQQKNPSD------RKNVQKVSLVV--DDMEGIAYTMNNEIHVSARYVNGYSGGDVKRE- 1581
Query: 169 FNNMAIVGAIQRAMARVWLWDGRSKASPRLLDGMVEYIAELAGFHRERLSGGVGESPECE 228
I G + + VW W G +A L+ G+ +++ RL S +
Sbjct: 1582 -----ITGVLFHQVCYVWQWYGNGEAPGGLIGGIADFV---------RLKANYAASHWRK 1719
Query: 229 DGRELWWDNKDPTHVARLLHYCEKYDKGFIQRLNEAMKDTWHDRMVGDVLGLKATKLCGL 288
G+ WD A L YC+ GF+ +LN+ M+ + D+ +LG +L
Sbjct: 1720 PGQGQRWDEGYDI-TAHFLDYCDSLKSGFVAQLNQLMRTGYSDQFFVQLLGKPVDQLWRD 1896
Query: 289 YNATY 293
Y A Y
Sbjct: 1897 YKAQY 1911
>TC228121 similar to UP|Q84ZV0 (Q84ZV0) R 14 protein, partial (9%)
Length = 532
Score = 35.4 bits (80), Expect = 0.031
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +3
Query: 234 WWDNKDPTHVARLLHYCEKYDKGFIQRLNEAMKDTWHDRMVGDVLGLKATKLCGLYNATY 293
W D T AR L YC GF+ +LN+ M+ + D+ +LG +L Y A Y
Sbjct: 3 WDQGYDVT--ARFLDYCNTLKSGFVAQLNKQMRTGYSDQFFVQLLGKTVDQLWQDYKAKY 176
>TC233520
Length = 405
Score = 34.3 bits (77), Expect = 0.068
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +2
Query: 214 RERLSGGVGESPECEDGRELW-WDNKDPTHVARLLHYCEKYDKGFIQRLNE 263
R RLS GE C+ G W + + P +AR H C D+GF+Q L+E
Sbjct: 98 RLRLSLFPGERKGCQVGATYWCYTCRQPICLARRDHICPYCDEGFLQELDE 250
>TC220327 similar to UP|Q8RY07 (Q8RY07) Serine/threonine protein kinase pk23,
partial (20%)
Length = 780
Score = 29.3 bits (64), Expect = 2.2
Identities = 22/63 (34%), Positives = 31/63 (48%), Gaps = 7/63 (11%)
Frame = -1
Query: 7 HFLFQPLLP-------HFNPSSYEINQQNDYNNSKFFIPNNNNIIHRILLILFVAIISIW 59
HF FQ L +FN Y N ND NN+ NNNN +I L++ +A+ ++
Sbjct: 582 HFSFQFKLKKEKKTQTNFNKYYYN-NNNNDNNNN-----NNNNNNKKISLLVILAVGVLY 421
Query: 60 ANY 62
NY
Sbjct: 420 LNY 412
>TC227282 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arabidopsis
thaliana;} , partial (61%)
Length = 1408
Score = 28.9 bits (63), Expect = 2.9
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 2/34 (5%)
Frame = +3
Query: 14 LPHFNPSSYE--INQQNDYNNSKFFIPNNNNIIH 45
L HF+PSS + QQ++ N+S IP+NNN H
Sbjct: 918 LRHFSPSSPQWCHQQQHNNNHSCHLIPSNNNEGH 1019
>TC206971 homologue to UP|RL35_EUPES (Q9M5L0) 60S ribosomal protein L35,
complete
Length = 768
Score = 28.9 bits (63), Expect = 2.9
Identities = 15/41 (36%), Positives = 26/41 (62%)
Frame = +2
Query: 180 RAMARVWLWDGRSKASPRLLDGMVEYIAELAGFHRERLSGG 220
R MAR+ +++ R+KA LL+ + + AELA +++GG
Sbjct: 47 RKMARIKVYELRNKAKAELLNQLKDLKAELALLRVAKVTGG 169
>TC213102 homologue to UP|O82155 (O82155) Dof zinc finger protein, partial
(34%)
Length = 451
Score = 28.1 bits (61), Expect = 4.9
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 9 LFQPLLPHFNPSSYEINQQNDYNNSKFFIPNNNNI 43
LFQP+ PHF + D N+KF NN N+
Sbjct: 13 LFQPMKPHFPEQEQLKCPRCDSTNTKFCYYNNYNL 117
>TC215984 similar to UP|Q9FMP3 (Q9FMP3) Dihydropyrimidinase
(Dihydropyrimidine amidohydrolase) , partial (90%)
Length = 1922
Score = 28.1 bits (61), Expect = 4.9
Identities = 14/67 (20%), Positives = 34/67 (49%)
Frame = -2
Query: 44 IHRILLILFVAIISIWANYEASKTFDIHIVNDTKDSLAGRRFTLFYVSNDKASRILLNTS 103
IH++ L + V + + W + AS +D H++++ + G T+F + + ++
Sbjct: 340 IHKLKL*VGVRVNAPWHDKLASGIYDRHLISNRNSGIDGNNDTIFDIDIGNLLVVSIHNG 161
Query: 104 SFVEQIL 110
+ ++Q L
Sbjct: 160 AALDQQL 140
>TC220860
Length = 1022
Score = 27.7 bits (60), Expect = 6.4
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 12 PLLPHFNPSSYEINQQNDYNNSKFFIPNNNN 42
P P + + +NDY+NSKF + NNN
Sbjct: 956 PYCPLCSWKQRRVGVENDYSNSKFCVNCNNN 864
>BE023716 weakly similar to GP|9758876|dbj disease resistance protein
{Arabidopsis thaliana}, partial (1%)
Length = 396
Score = 27.7 bits (60), Expect = 6.4
Identities = 14/53 (26%), Positives = 30/53 (56%)
Frame = +1
Query: 90 VSNDKASRILLNTSSFVEQILYPNGNNDNIDIKNKKIIKSVTLRLARQNLDTI 142
V N K+ + L F++ +L+P+ + + KK++ L+L +Q+L++I
Sbjct: 97 VGNCKSLQTLPKLPHFLKTVLFPSAAVEQLAENRKKVLFLNCLKLDQQSLESI 255
>TC230207 similar to UP|Q9LTR6 (Q9LTR6) Gb|AAF36750.1, partial (28%)
Length = 823
Score = 27.3 bits (59), Expect = 8.4
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = -1
Query: 8 FLFQPLLPHFNPSSYEINQQNDYNNSKFFIPNNNNIIHRILLIL 51
FLF +LP + NQ + +S+FF P N I ++I++IL
Sbjct: 550 FLFLFILPFLRAGIFFRNQHHKTVSSQFF*PPLNLIGNKIIVIL 419
>CO984054
Length = 817
Score = 27.3 bits (59), Expect = 8.4
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +3
Query: 96 SRILLNTSSFVEQILYPNGNNDNIDIKNKKIIKSVTLRLARQNLDTITT 144
SRILLN+SS V + + G + S TL L +N+ I T
Sbjct: 549 SRILLNSSSVVNSLTHSTGQRSSSSSDKPS*YSSATLILLGKNVCLIFT 695
>TC231346 weakly similar to UP|Q6QM04 (Q6QM04) LRR protein WM1.2, partial (14%)
Length = 1790
Score = 27.3 bits (59), Expect = 8.4
Identities = 26/95 (27%), Positives = 42/95 (43%), Gaps = 10/95 (10%)
Frame = +1
Query: 18 NPSSYEINQQNDYNNSK---FFIPNNNNIIHRILLILFVAIISIWAN-------YEASKT 67
NPSSY YN+ K +P + + +R LIL V +I + +N E SK
Sbjct: 1312 NPSSYSYGSDFSYNHYKETLVLVPKKDELEYRDNLIL-VRMIDLSSNKLSGAIPSEISKL 1488
Query: 68 FDIHIVNDTKDSLAGRRFTLFYVSNDKASRILLNT 102
+ +N +++ L+G + ND LL +
Sbjct: 1489 SALRFLNLSRNHLSGE------IPNDMGKMKLLES 1575
>CD418668
Length = 579
Score = 27.3 bits (59), Expect = 8.4
Identities = 17/49 (34%), Positives = 23/49 (46%)
Frame = +2
Query: 96 SRILLNTSSFVEQILYPNGNNDNIDIKNKKIIKSVTLRLARQNLDTITT 144
SRILLN+SS V + + G + S TL L +N+ I T
Sbjct: 431 SRILLNSSSVVNSLTHSTGQRSSSSSDKPS*YSSATLILLGKNVCLIFT 577
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.320 0.137 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,040,940
Number of Sequences: 63676
Number of extensions: 182798
Number of successful extensions: 1003
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 994
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 996
length of query: 294
length of database: 12,639,632
effective HSP length: 97
effective length of query: 197
effective length of database: 6,463,060
effective search space: 1273222820
effective search space used: 1273222820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147496.3