BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147471.4 + phase: 0
(274 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC205017 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partia... 291 3e-79
TC205016 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partia... 290 4e-79
TC205018 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partia... 208 2e-54
TC228744 similar to UP|Q94A21 (Q94A21) AT5g15330/F8M21_220, part... 175 2e-44
TC234042 weakly similar to UP|Q940E0 (Q940E0) IDS-4-like protein... 166 1e-41
TC205019 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partia... 165 2e-41
BE803757 145 1e-35
AW277461 similar to GP|11994328|dbj receptor protein-like {Arabi... 32 0.31
TC212419 similar to GB|AAO42875.1|28416689|BT004629 At3g50340 {A... 30 1.2
TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotrans... 30 1.5
BU082790 30 1.5
TC213265 similar to UP|Q940Y1 (Q940Y1) AT4g22540/F7K2_120, parti... 29 2.6
BE801264 28 4.5
TC231346 weakly similar to UP|Q6QM04 (Q6QM04) LRR protein WM1.2,... 27 7.6
TC223710 similar to UP|Q8LDF8 (Q8LDF8) RING zinc finger protein-... 27 7.6
BF598060 similar to PIR|S19190|S19 trypsin inhibitor B (Kunitz) ... 27 10.0
>TC205017 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (80%)
Length = 1172
Score = 291 bits (744), Expect = 3e-79
Identities = 154/266 (57%), Positives = 192/266 (71%)
Frame = +1
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKRRRLDDDGGAEGEV 60
MKF K L++QIE+TLP+WRDKFLSYK+LKK+LK P +R D ++
Sbjct: 121 MKFGKSLSSQIEKTLPEWRDKFLSYKELKKKLKQFDPPAAADERPGKRLKSDAVPDAADM 300
Query: 61 TKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIELMTVGREIVDF 120
+KE DF LLE E++KFN FFVEKEEEY+I+ KELQD+VA K S E+M + +EIVDF
Sbjct: 301 SKEESDFRNLLENELDKFNTFFVEKEEEYIIRLKELQDRVAKVKDSSEEMMKIHKEIVDF 480
Query: 121 HGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECE 180
HGEMVLLENYSALNYTGLVKI+KKYDKRTGAL+RLPFIQ VL QPFF D+L KLVKECE
Sbjct: 481 HGEMVLLENYSALNYTGLVKILKKYDKRTGALIRLPFIQKVLQQPFFTTDLLYKLVKECE 660
Query: 181 VMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMENIFIKL 240
ML +FP + P S T+ E T+T ++ + +PKE +EI+ ME++++K
Sbjct: 661 TMLDHLFPVNDPAPVSTETTPQAEGFDPSTSTTTKSDGLV--IPKELAEIEYMESLYMKS 834
Query: 241 TTSALDTLKEIRGGSSTVSIYSLPPL 266
T SAL L+EIR GSSTVS++SLPPL
Sbjct: 835 TVSALHVLQEIRSGSSTVSMFSLPPL 912
>TC205016 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (88%)
Length = 1280
Score = 290 bits (743), Expect = 4e-79
Identities = 159/276 (57%), Positives = 197/276 (70%), Gaps = 10/276 (3%)
Frame = +1
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKR--RRLDDDGGAE- 57
MKF K L++QIE+TLP+WRDKFLSYK+LKK+LK P S+ +R +RL D G
Sbjct: 109 MKFGKSLSSQIEKTLPEWRDKFLSYKELKKKLKQFDPPAPASAADERPGKRLKTDAGNAD 288
Query: 58 -------GEVTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIEL 110
+++KE DF LLE E++KFN FFVEKEEEY+I+ KELQD VA K S E+
Sbjct: 289 ADAVSDASDMSKEESDFRNLLENELDKFNTFFVEKEEEYIIRLKELQDSVAQVKGSREEM 468
Query: 111 MTVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKID 170
M + +EIVDFHGEMVLLENYSALNYTGLVKI+KKYDKRTGAL+RLPFIQ VL QPFF D
Sbjct: 469 MKIHKEIVDFHGEMVLLENYSALNYTGLVKILKKYDKRTGALIRLPFIQKVLQQPFFITD 648
Query: 171 VLNKLVKECEVMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEI 230
+L KLVKECE ML +FP + P S T+ E T+T ++ L +PKE +EI
Sbjct: 649 LLYKLVKECETMLDRLFPVNDPAPVSSETTPQAEGFDPSTSTTTKSDGLL--IPKELAEI 822
Query: 231 QNMENIFIKLTTSALDTLKEIRGGSSTVSIYSLPPL 266
+ ME++++K T SAL L+EIR GSSTVS++SLPPL
Sbjct: 823 EYMESLYMKSTVSALHVLQEIRSGSSTVSMFSLPPL 930
>TC205018 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (59%)
Length = 555
Score = 208 bits (530), Expect = 2e-54
Identities = 110/182 (60%), Positives = 133/182 (72%)
Frame = +1
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKRRRLDDDGGAEGEV 60
MKF K L+NQIE+TLP WRDKFLSYK+LKK+LKL+ PK I+ + + G +
Sbjct: 10 MKFGKSLSNQIEKTLPQWRDKFLSYKELKKKLKLVEPKPINGVEERPTKRARHEGDIIIM 189
Query: 61 TKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIELMTVGREIVDF 120
+KE DF +E E+ KFN FFVEKEEE +IK KELQD+VA K+S+ +LM + +EIVDF
Sbjct: 190 SKEETDFRNSIEQELHKFNTFFVEKEEECIIKLKELQDRVAKVKNSNEQLMQIRKEIVDF 369
Query: 121 HGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECE 180
HGEMVLLENYSALNY GLV I+ YD RTGAL+RLPFIQ VL FF D+L KLV EC+
Sbjct: 370 HGEMVLLENYSALNYIGLVNILMSYD*RTGALIRLPFIQMVLQSAFFTTDLLYKLVNECD 549
Query: 181 VM 182
M
Sbjct: 550 TM 555
>TC228744 similar to UP|Q94A21 (Q94A21) AT5g15330/F8M21_220, partial (69%)
Length = 1196
Score = 175 bits (443), Expect = 2e-44
Identities = 110/286 (38%), Positives = 162/286 (56%), Gaps = 18/286 (6%)
Frame = +1
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKRRRLDDDGGAEGEV 60
MKF K +E T+P+WRDKFL YK LKK LK +P + L +
Sbjct: 67 MKFGKEFKTHLEDTIPEWRDKFLCYKPLKKLLKQNLPSITTAITDIPINLPLHLLQQPSS 246
Query: 61 TKEVKD-FLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAK---------SSDIE- 109
+ ++ F+R+L E+EKFN F+V+KEEE+VI+++EL++++ K +SD E
Sbjct: 247 PQLLQAWFVRILNEELEKFNDFYVDKEEEFVIRFQELKERIECLKEKSSQGEVYTSDCEF 426
Query: 110 ---LMTVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPF 166
+M + +++V HGEMVLL+NYS+LN+ GLVKI+KKYDKRTG LLRLPF Q VL QPF
Sbjct: 427 SEEMMDIRKDLVTIHGEMVLLKNYSSLNFAGLVKILKKYDKRTGGLLRLPFTQLVLRQPF 606
Query: 167 FKIDVLNKLVKECEVMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKE 226
F + L +LV ECE L ++FP + QS E N+++ +D
Sbjct: 607 FTTEPLTRLVHECEENLELLFPLQAEVIQSTPPPE------------NQSRPPVDSATNA 750
Query: 227 FSEIQNM---ENIFI-KLTTSALDTLKEIRGGSSTVSIYSLPPLHS 268
E + E ++I + T +A+ +K ++ SST + +S L S
Sbjct: 751 LPEASSTLGDETVYIYRSTLAAMRAIKGLQKASSTSNPFSFSSLFS 888
>TC234042 weakly similar to UP|Q940E0 (Q940E0) IDS-4-like protein (Fragment),
partial (48%)
Length = 569
Score = 166 bits (419), Expect = 1e-41
Identities = 91/132 (68%), Positives = 102/132 (76%)
Frame = +1
Query: 137 GLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSIIFPKSGPLGQS 196
GLVKIIKKY TGALLRLPFIQ+VLNQPFFKIDVLNKLVKECEV+LSI+F P
Sbjct: 19 GLVKIIKKYXXXTGALLRLPFIQEVLNQPFFKIDVLNKLVKECEVILSILFNNDWP---- 186
Query: 197 LSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMENIFIKLTTSALDTLKEIRGGSS 256
S S FEE +TT NE+K TL HVPKE +EI+NMEN F KLT SAL +L+EIRG S
Sbjct: 187 -SISGDFEEDEYGSTTGNESKATLMHVPKELAEIENMENTFTKLTLSALRSLEEIRGRS* 363
Query: 257 TVSIYSLPPLHS 268
VSI+SLPPLH+
Sbjct: 364 KVSIFSLPPLHN 399
>TC205019 similar to UP|Q8LBH4 (Q8LBH4) Ids4-like protein, partial (50%)
Length = 959
Score = 165 bits (418), Expect = 2e-41
Identities = 90/159 (56%), Positives = 114/159 (71%), Gaps = 1/159 (0%)
Frame = +2
Query: 116 EIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKL 175
EIVDFHGEM LLENYSALNY GLVKI+KKYDKRTGAL+RLPFIQ VL QPFF D+L KL
Sbjct: 2 EIVDFHGEMFLLENYSALNYIGLVKILKKYDKRTGALIRLPFIQKVLQQPFFTTDLLYKL 181
Query: 176 VKECEVMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEI-QNME 234
VKECE ML+ +FP + P + + E T+T+ ++ + L +PKE + ++E
Sbjct: 182 VKECETMLNHLFPVNDPSTSGEAPPQAEEGCDASTSTSTKSSDDL-LMPKELAAANHHIE 358
Query: 235 NIFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 273
++++K T SAL L+EIR GSSTVS++SLPPL L E
Sbjct: 359 SLYMKSTISALHVLQEIRKGSSTVSMFSLPPLQISGLEE 475
>BE803757
Length = 520
Score = 145 bits (367), Expect = 1e-35
Identities = 83/172 (48%), Positives = 108/172 (62%), Gaps = 10/172 (5%)
Frame = +2
Query: 2 KFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKR--RRLDDDGGAE-- 57
KF K L++QIE+TLP+WR+KFLSYK+LKK+LK P S+ + +RL + G
Sbjct: 2 KFGKSLSSQIEKTLPEWRNKFLSYKELKKKLKQFDPPTPASAADEHPGKRLKTNTGNTDT 181
Query: 58 ------GEVTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIELM 111
++KE +F LLE E+ KFN FF+E EE Y+I ELQ VA S ++M
Sbjct: 182 NTISNASNMSKEESNFQNLLENELNKFNTFFIENEE*YIITLXELQHXVAQFNGSREDMM 361
Query: 112 TVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLN 163
+ EIV+FH E+VLLENY LNYT LV I+K YD T L+ LPFIQ VL+
Sbjct: 362 KIHNEIVNFHREIVLLENYITLNYTRLVNILKYYDNETNTLIHLPFIQKVLH 517
>AW277461 similar to GP|11994328|dbj receptor protein-like {Arabidopsis
thaliana}, partial (5%)
Length = 440
Score = 32.0 bits (71), Expect = 0.31
Identities = 15/35 (42%), Positives = 24/35 (67%)
Frame = +3
Query: 1 MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLI 35
+KF K L Q+ +P+W++ F++Y LKKQ+K I
Sbjct: 102 VKFSKELEAQL---IPEWKEAFVNYWQLKKQIKRI 197
>TC212419 similar to GB|AAO42875.1|28416689|BT004629 At3g50340 {Arabidopsis
thaliana;} , partial (57%)
Length = 925
Score = 30.0 bits (66), Expect = 1.2
Identities = 39/163 (23%), Positives = 70/163 (42%), Gaps = 3/163 (1%)
Frame = +3
Query: 41 DSSCSKRRRLDDDGGAEGEVTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKV 100
D++ KRRR + E R E+ K G+ EEY+ + +
Sbjct: 297 DAATDKRRRNSSSSSLSASSSPE-----RFFEMPRSKVPGWV----EEYIGQIGSVLKAG 449
Query: 101 AWAKSSDIELMTVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQD 160
W++S E++ V F GEMV+L+N + L+ ++ K DK + +L + + +
Sbjct: 450 GWSESDINEMVEVSASGF-FEGEMVVLDNQALLD-----ALLLKADKFSDSLRKAGWSSE 611
Query: 161 VLNQPF---FKIDVLNKLVKECEVMLSIIFPKSGPLGQSLSTS 200
+++ F+ + K K+ L+ K L QS+S S
Sbjct: 612 EVSEALGFDFRPEKERKPAKKLSPQLAERIEK---LAQSVSRS 731
>TC206671 weakly similar to UP|Q8L5A7 (Q8L5A7) Steroid sulfotransferase-like
protein (At5g07010), partial (40%)
Length = 1308
Score = 29.6 bits (65), Expect = 1.5
Identities = 25/98 (25%), Positives = 43/98 (43%), Gaps = 9/98 (9%)
Frame = +3
Query: 12 EQTLPDWRDK--------FLSYKDLKKQLKLIVPKEID-SSCSKRRRLDDDGGAEGEVTK 62
+Q L W++ FL Y+DLKK + V + + C + DG E +
Sbjct: 702 DQMLGYWKESIARPSKVLFLKYEDLKKDVNFHVKRIAEFLGCPFTSEEEGDGTIESII-- 875
Query: 63 EVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKV 100
++ F ++ E+E K F E +Y+ + E+ D V
Sbjct: 876 KLCSFEKMKELEANKSGTFARNFERKYLFRKAEMGDWV 989
>BU082790
Length = 431
Score = 29.6 bits (65), Expect = 1.5
Identities = 15/42 (35%), Positives = 24/42 (56%)
Frame = +3
Query: 59 EVTKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKV 100
E K ++ ++ E +E F+EKE+E K +EL+DKV
Sbjct: 6 EKIKTLEGMIKSKETALEMSTSSFLEKEKELQSKIEELEDKV 131
>TC213265 similar to UP|Q940Y1 (Q940Y1) AT4g22540/F7K2_120, partial (15%)
Length = 759
Score = 28.9 bits (63), Expect = 2.6
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +3
Query: 192 PLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMENIFIKLTTSALDTLKEI 251
P SLST + + E T N KE + EFSE Q + + ++ LDT++++
Sbjct: 345 PNNISLSTERLKIRLLEEGTGENLVKECEQILLTEFSEKQGQLEVLCQERSNLLDTIRQL 524
>BE801264
Length = 384
Score = 28.1 bits (61), Expect = 4.5
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -3
Query: 7 LNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKRRRLDDD 53
L +Q LPD K+ +Y L+L PKE+D+ + R L+ D
Sbjct: 229 LGHQPNLNLPDQVAKYSTYAMFPYHLQLHYPKEMDAYPGRGRYLETD 89
>TC231346 weakly similar to UP|Q6QM04 (Q6QM04) LRR protein WM1.2, partial (14%)
Length = 1790
Score = 27.3 bits (59), Expect = 7.6
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = +1
Query: 1 MKFWKILNNQIEQTLPDW 18
MKF + NNQ+ T+PDW
Sbjct: 1069 MKFIDMGNNQLSDTIPDW 1122
>TC223710 similar to UP|Q8LDF8 (Q8LDF8) RING zinc finger protein-like,
partial (49%)
Length = 465
Score = 27.3 bits (59), Expect = 7.6
Identities = 23/80 (28%), Positives = 37/80 (45%), Gaps = 2/80 (2%)
Frame = +1
Query: 90 VIKWKELQDKVAWAKSSDIELMTVGREIVDFHGEMVL--LENYSALNYTGLVKIIKKYDK 147
V+K+ E++ V A+S + L +F GE + L N + + G + YD+
Sbjct: 214 VVKFSEMEMAVEAAESCAVCLY-------EFEGEDEIRRLTNCRHIFHRGCLDRWMGYDQ 372
Query: 148 RTGALLRLPFIQDVLNQPFF 167
RT L R PFI + F+
Sbjct: 373 RTCPLCRTPFIPHHMQAAFY 432
>BF598060 similar to PIR|S19190|S19 trypsin inhibitor B (Kunitz) precursor -
soybean, partial (47%)
Length = 316
Score = 26.9 bits (58), Expect = 10.0
Identities = 21/78 (26%), Positives = 35/78 (43%), Gaps = 2/78 (2%)
Frame = -2
Query: 190 SGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMEN--IFIKLTTSALDT 247
+GP G+S +T +ANE+ +L +P I+N++ + I L +S D+
Sbjct: 267 AGPSGRSSTTDHSVGIPTHSIISANESNLSLKGLPSAIQRIRNVDEMIVTIPL*SSLQDS 88
Query: 248 LKEIRGGSSTVSIYSLPP 265
I S V +PP
Sbjct: 87 TTVIGHVSFPVGGALIPP 34
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.136 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,381,303
Number of Sequences: 63676
Number of extensions: 115876
Number of successful extensions: 618
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 608
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 609
length of query: 274
length of database: 12,639,632
effective HSP length: 96
effective length of query: 178
effective length of database: 6,526,736
effective search space: 1161759008
effective search space used: 1161759008
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147471.4