BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147178.5 + phase: 0 /pseudo
(676 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230687 weakly similar to UP|Q9FYW6 (Q9FYW6) BAC19.10, partial ... 157 9e-77
BF066314 weakly similar to GP|9858778|gb|A BAC19.10 {Lycopersico... 50 2e-06
TC213566 weakly similar to UP|Q79FY9 (Q79FY9) PE-PGRS FAMILY PRO... 36 0.064
CO979245 33 0.41
TC214997 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (... 32 0.70
TC204652 similar to UP|H1_PEA (P08283) Histone H1, partial (81%) 32 0.70
BQ299453 weakly similar to GP|5734705|gb|A F24J5.4 {Arabidopsis ... 32 0.92
CF922863 31 1.6
TC221636 similar to UP|Q79G09 (Q79G09) PE-PGRS FAMILY PROTEIN, p... 31 1.6
CO979043 31 2.0
TC232564 31 2.0
TC214998 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (... 31 2.0
CO983431 31 2.0
TC226468 weakly similar to UP|O65450 (O65450) Glycine-rich prote... 31 2.0
TC207098 similar to UP|Q8L7Z6 (Q8L7Z6) AT3g54680/T5N23_40, parti... 30 2.7
TC230645 weakly similar to UP|Q9EQF1 (Q9EQF1) GABA-A epsilon sub... 30 2.7
TC209484 30 2.7
TC220421 weakly similar to UP|GP1_CHLRE (Q9FPQ6) Vegetative cell... 30 2.7
TC207097 similar to UP|Q8L7Z6 (Q8L7Z6) AT3g54680/T5N23_40, parti... 30 3.5
TC215924 similar to GB|AAS68109.1|45592910|BT011785 At2g38360 {A... 30 3.5
>TC230687 weakly similar to UP|Q9FYW6 (Q9FYW6) BAC19.10, partial (9%)
Length = 1293
Score = 157 bits (398), Expect(2) = 9e-77
Identities = 78/110 (70%), Positives = 95/110 (85%)
Frame = +1
Query: 563 VCSFQLICQELRGLALAQTMLPKGGSKMSVDAANSLDVPQDELKAILGEVACDIHGCYVL 622
VCSF+LIC+ LR +A+++ ML KG SKM+VDAA+SLD P +EL A++ EVAC+I+G YVL
Sbjct: 652 VCSFKLICEGLREMAVSKAMLSKGDSKMAVDAAHSLDGPHNELMAVISEVACEINGSYVL 831
Query: 623 KSAQNDPFRDVVIDMLRGSGPNGKLKKAEILEAARRKLGRDVPNNEYIKV 672
KS+ +DPFRDVVIDML GSGPN KLKKAEILEAARRKLGR+V +NEY KV
Sbjct: 832 KSSPDDPFRDVVIDMLCGSGPNAKLKKAEILEAARRKLGREVASNEYTKV 981
Score = 148 bits (374), Expect(2) = 9e-77
Identities = 99/214 (46%), Positives = 128/214 (59%), Gaps = 4/214 (1%)
Frame = +2
Query: 353 RALHFTVLVLSSTLLLNALMTNC*VFWRSKHNCCGVTGFQEVACCFLQAV*KVWQEIISL 412
R +HF V VL STLL N N * S CG G Q+V C L V K+W EII
Sbjct: 20 RIVHFIVSVLLSTLLQNIPRMNF*DSCSSMQYWCGDYGLQKVHCYILMVVWKLWPEIIPY 199
Query: 413 *GFTRV*QLSLMS*EGSVAVNLVIA*NTL*CNLLWKSLI*----NPKKIIGSSKSFLTSP 468
RV*+ S + * VNL+I * T * L WK +I + + GSS+SFLTS
Sbjct: 200 YCLARV*KFSFLM*VFE-EVNLLIV*ETA*RYLAWKEMILINLLENQILTGSSESFLTSH 376
Query: 469 S*KNFQLLLKNKKKFLRSWKMR*IVLSVLLEEKENLKVL*QTVVSTMLFLHQQILIHGQQ 528
S + Q+LLKN+K F + WK +V +LE + +K+L*QT V T+ F++Q++ I+G Q
Sbjct: 377 SKNSIQILLKNRKNFSKVWKNSYLVFLRMLENVKLVKML*QTKV*TLRFINQKVQING*Q 556
Query: 529 VWVKILLGA*PCQVKLVMLCQLPSRSFFKLTKFA 562
VW + LL *PCQ+K MLCQLP R+FF+LTKFA
Sbjct: 557 VWAECLLRK*PCQMKPDMLCQLP*RNFFRLTKFA 658
>BF066314 weakly similar to GP|9858778|gb|A BAC19.10 {Lycopersicon
esculentum}, partial (4%)
Length = 308
Score = 50.4 bits (119), Expect = 2e-06
Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 3/44 (6%)
Frame = +3
Query: 1 MDFDDLD-APVKP--RVSRFAPKSSKFKPKKEEPSLVPKSEPPP 41
MDFDDL+ AP+K RVS+FAPKSSK KPK + PK EP P
Sbjct: 171 MDFDDLEEAPIKATSRVSKFAPKSSKLKPKPKSEPAPPKPEPQP 302
>TC213566 weakly similar to UP|Q79FY9 (Q79FY9) PE-PGRS FAMILY PROTEIN,
partial (4%)
Length = 676
Score = 35.8 bits (81), Expect = 0.064
Identities = 25/67 (37%), Positives = 30/67 (44%), Gaps = 10/67 (14%)
Frame = -3
Query: 8 APVKPRVSRFA------PKSSKFKPKK----EEPSLVPKSEPPPLASAKTEPQEIDLTAP 57
APVKP V + P S K PKK P +P ++ PPL + K P AP
Sbjct: 581 APVKPPVPKVTPALSPKPPSPKIPPKKAPISPPPLPLPPADAPPLPTPKVSP--TPSKAP 408
Query: 58 TPVKHEP 64
TP K P
Sbjct: 407 TPAKETP 387
>CO979245
Length = 730
Score = 33.1 bits (74), Expect = 0.41
Identities = 19/54 (35%), Positives = 22/54 (40%), Gaps = 2/54 (3%)
Frame = +3
Query: 8 APVKPRVSRFAPKS--SKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTP 59
AP P + APK P K P P PPP A + P +D T P P
Sbjct: 231 APKPPPGAAAAPKPLPGAAAPPKPPPGAAPPPNPPPEAIPENPPLPVDATLPKP 392
Score = 30.4 bits (67), Expect = 2.7
Identities = 21/62 (33%), Positives = 27/62 (42%), Gaps = 2/62 (3%)
Frame = +3
Query: 8 APVKPRVSRFAPKSSK--FKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPN 65
AP P V APK + P K P + +PPP A+A +P A P K P
Sbjct: 141 APANPPVEEAAPKPTPGAAAPAKPPPGVAAAPKPPPGAAAAPKPLP---GAAAPPKPPPG 311
Query: 66 AS 67
A+
Sbjct: 312 AA 317
>TC214997 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (At3g10190),
partial (45%)
Length = 1107
Score = 32.3 bits (72), Expect = 0.70
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = +1
Query: 14 VSRFAPKSSKFKPK----KEEPSLVPKSEPPPLASAKT 47
+S PK+ K PK K+EPSLV +S+PP S +
Sbjct: 103 ISTINPKNLKLSPKRFFRKKEPSLVSRSDPPSFGSGSS 216
>TC204652 similar to UP|H1_PEA (P08283) Histone H1, partial (81%)
Length = 1347
Score = 32.3 bits (72), Expect = 0.70
Identities = 22/70 (31%), Positives = 33/70 (46%), Gaps = 1/70 (1%)
Frame = +3
Query: 11 KPRVSRFAPKSSKFK-PKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVK 69
KP K++K K P K +P+ PK++ P A +P+ P P +P A+ K
Sbjct: 567 KPAAKAKDTKATKSKAPAKPKPAAKPKAKAPAKAKPAAKPKASAAAKPKPAAAKPKAA-K 743
Query: 70 TDAESKIEGK 79
A+SK K
Sbjct: 744 PAAKSKPAAK 773
>BQ299453 weakly similar to GP|5734705|gb|A F24J5.4 {Arabidopsis thaliana},
partial (20%)
Length = 423
Score = 32.0 bits (71), Expect = 0.92
Identities = 20/57 (35%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Frame = +3
Query: 8 APVKPRVSRFAPKSSKFKPKKEEPS---LVPKSEPPPLASAKTEPQEIDLTAPTPVK 61
A V P + AP SS P ++P + P + PPP A P APTP K
Sbjct: 246 ALVTPPAPKVAPVSSPKAPPPQQPKPSPVSPPTSPPPPPVASPPPLPPPKIAPTPAK 416
>CF922863
Length = 392
Score = 31.2 bits (69), Expect = 1.6
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +1
Query: 13 RVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTP 59
+V F PK + KP + + PK+ PPP + E ++ L+ P P
Sbjct: 118 QVQHFKPKKKRKKPPRAQKE--PKTPPPPKKRPRGEKKKKTLSPPNP 252
>TC221636 similar to UP|Q79G09 (Q79G09) PE-PGRS FAMILY PROTEIN, partial (5%)
Length = 756
Score = 31.2 bits (69), Expect = 1.6
Identities = 19/50 (38%), Positives = 23/50 (46%), Gaps = 4/50 (8%)
Frame = -1
Query: 19 PKSSKFKPKK----EEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEP 64
P S K PKK P +P ++ PPL + K P APTP K P
Sbjct: 750 PPSPKIPPKKAPISPPPLPLPPADAPPLPTPKVSP--TPSKAPTPAKETP 607
>CO979043
Length = 764
Score = 30.8 bits (68), Expect = 2.0
Identities = 15/41 (36%), Positives = 19/41 (45%)
Frame = +2
Query: 35 PKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVKTDAESK 75
PKS PPP AS + EP + T H P + + SK
Sbjct: 224 PKSSPPPFASPQQEPLLLQQTTSLFFSHHPGITPSAISASK 346
>TC232564
Length = 730
Score = 30.8 bits (68), Expect = 2.0
Identities = 21/68 (30%), Positives = 30/68 (43%), Gaps = 4/68 (5%)
Frame = -3
Query: 12 PRVSRFAPKSSKFK----PKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNAS 67
PR+ AP SS+ P+ PS VP + P A KT T P+P + S
Sbjct: 644 PRLQAAAPPSSRSSSASPPRSISPSAVPPAPSPTAAETKT-----TTTTPSPTRFRRRDS 480
Query: 68 VKTDAESK 75
+ + A S+
Sbjct: 479 LDSAAPSR 456
>TC214998 similar to UP|Q9SS31 (Q9SS31) Calmodulin-like protein (At3g10190),
partial (48%)
Length = 1097
Score = 30.8 bits (68), Expect = 2.0
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 4/61 (6%)
Frame = +3
Query: 14 VSRFAPKSSKFKPK----KEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVK 69
+S PK+ K PK K+E SLV +S+PP S + + H+P+A +
Sbjct: 81 ISSINPKNLKLSPKRFFRKKERSLVSRSDPPSFGSGSSSDD-------STTNHKPSAGNQ 239
Query: 70 T 70
T
Sbjct: 240 T 242
>CO983431
Length = 783
Score = 30.8 bits (68), Expect = 2.0
Identities = 20/61 (32%), Positives = 24/61 (38%), Gaps = 3/61 (4%)
Frame = +1
Query: 9 PVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQ---EIDLTAPTPVKHEPN 65
P P R + +S KP P P S+P ASA PQ P P + EP
Sbjct: 289 PF*PASQRLSKPTSALKPHHSHPPTSPPSQPVAAASATEAPQARLPSHARIPQPSQAEPK 468
Query: 66 A 66
A
Sbjct: 469 A 471
>TC226468 weakly similar to UP|O65450 (O65450) Glycine-rich protein, partial
(20%)
Length = 1041
Score = 30.8 bits (68), Expect = 2.0
Identities = 23/69 (33%), Positives = 27/69 (38%), Gaps = 11/69 (15%)
Frame = -2
Query: 8 APVKPRVSRFAPKSSKFKPKKEEPSLVPKSEP-----------PPLASAKTEPQEIDLTA 56
APVKP + AP SS P P +P +P PP A P A
Sbjct: 797 APVKPSAPKVAPASSPKAP----PPQIPIPQPPKPSPVSPPPLPPPPVASPPPLPPPKIA 630
Query: 57 PTPVKHEPN 65
PTP K P+
Sbjct: 629 PTPAKTPPS 603
>TC207098 similar to UP|Q8L7Z6 (Q8L7Z6) AT3g54680/T5N23_40, partial (8%)
Length = 733
Score = 30.4 bits (67), Expect = 2.7
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 12 PRVSRFAPKSSKFKPKKEEPSLVPKSEPPP 41
P +S PK++KF P P VP+ E PP
Sbjct: 12 PLLSPQPPKTNKFVPPHLRPGFVPREEAPP 101
>TC230645 weakly similar to UP|Q9EQF1 (Q9EQF1) GABA-A epsilon subunit splice
variant (Fragment), partial (8%)
Length = 464
Score = 30.4 bits (67), Expect = 2.7
Identities = 22/101 (21%), Positives = 43/101 (41%)
Frame = +2
Query: 19 PKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNASVKTDAESKIEG 78
P+ +++P P++EP P + +T+PQ + TP + EPN +++
Sbjct: 116 PEPESQNGHEQDPEPQPETEPVP--TEQTQPQLEPKSKSTP-EPEPNPQPESEPV----- 271
Query: 79 KVDSRNVDVEMTEAVDGSREVNPMDEEDTVVREIDVYFSPS 119
+ +E + VN D +T + + Y +PS
Sbjct: 272 PTEQTQAQLEPKSGSEADPAVNDADLRETTIHSNETYANPS 394
>TC209484
Length = 1093
Score = 30.4 bits (67), Expect = 2.7
Identities = 27/100 (27%), Positives = 44/100 (44%)
Frame = +2
Query: 125 KLYVMQYPLRPSWRPYDLDEQCEEVRLKPGTSEVEVDLSIDLESYNVEKATADKLKYTKQ 184
K Y+ P + R DL++ E+ L G + + DLS+ + K ++ +
Sbjct: 146 KAYLKIAPPSSNLRLRDLEQLTREMELAVGEATQDSDLSM---------SALQKRRHMEA 298
Query: 185 TLSTTWKPPPANGCAVGLLMGDKLHLHPIHAVVQLRPSRH 224
+LS ++ P C+ MG KLH A Q+R RH
Sbjct: 299 SLSKVYRAFP--DCSA---MGAKLHTMQRQAEEQVRSQRH 403
>TC220421 weakly similar to UP|GP1_CHLRE (Q9FPQ6) Vegetative cell wall
protein gp1 precursor (Hydroxyproline-rich glycoprotein
1), partial (13%)
Length = 1409
Score = 30.4 bits (67), Expect = 2.7
Identities = 23/76 (30%), Positives = 31/76 (40%)
Frame = +3
Query: 8 APVKPRVSRFAPKSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPTPVKHEPNAS 67
A P S AP +S PS P S P P ++A + P T+P+P P+ +
Sbjct: 288 ATSSPSASPTAPPTSPSPSPPSSPS--PPSAPSPSSTAPSPPSASPPTSPSP--SPPSPA 455
Query: 68 VKTDAESKIEGKVDSR 83
A S G SR
Sbjct: 456 STASANSPASGSPTSR 503
>TC207097 similar to UP|Q8L7Z6 (Q8L7Z6) AT3g54680/T5N23_40, partial (24%)
Length = 882
Score = 30.0 bits (66), Expect = 3.5
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +2
Query: 12 PRVSRFAPKSSKFKPKKEEPSLVPKSEPPP 41
P +S PK+ KF P P VP+ E PP
Sbjct: 410 PLLSPQPPKTDKFVPPHLRPGFVPREEAPP 499
>TC215924 similar to GB|AAS68109.1|45592910|BT011785 At2g38360 {Arabidopsis
thaliana;} , partial (68%)
Length = 947
Score = 30.0 bits (66), Expect = 3.5
Identities = 16/39 (41%), Positives = 20/39 (51%)
Frame = +3
Query: 20 KSSKFKPKKEEPSLVPKSEPPPLASAKTEPQEIDLTAPT 58
+SS P+ PS PK PP ASA+T P T P+
Sbjct: 216 RSSGTAPRSRSPSPSPK---PPSASARTSPTSASTTTPS 323
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.327 0.140 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,180,653
Number of Sequences: 63676
Number of extensions: 435372
Number of successful extensions: 3702
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 3443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3657
length of query: 676
length of database: 12,639,632
effective HSP length: 104
effective length of query: 572
effective length of database: 6,017,328
effective search space: 3441911616
effective search space used: 3441911616
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC147178.5