BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147178.12 + phase: 0
(163 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC209740 similar to GB|AAL31103.1|16974355|AY060490 AT3g50680/T3... 249 3e-67
TC215177 homologue to GB|AAP31934.1|30387537|BT006590 At2g35860 ... 30 0.67
TC204630 similar to UP|Q8RWF0 (Q8RWF0) 26S proteasome subunit-li... 29 1.1
AI988139 28 1.5
BU549976 28 1.5
TC230140 similar to UP|O03296 (O03296) Cytochrome b (Fragment), ... 27 4.3
TC204995 homologue to UP|V0D2_ARATH (Q9LHA4) Probable vacuolar A... 26 7.4
BU547639 26 7.4
BU082637 homologue to GP|3204132|emb| extensin {Cicer arietinum}... 26 7.4
TC204369 similar to GB|BAB09857.1|9759402|AB008268 phi-1-like pr... 26 9.6
TC231569 similar to UP|Q9LIC8 (Q9LIC8) Emb|CAA64867.1, partial (... 26 9.6
TC226993 homologue to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 deh... 26 9.6
TC230206 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {A... 26 9.6
CD415868 26 9.6
BU760650 similar to GP|28851007|gb tRNA nucleotidyltransferase {... 26 9.6
>TC209740 similar to GB|AAL31103.1|16974355|AY060490 AT3g50680/T3A5_60
{Arabidopsis thaliana;} , partial (77%)
Length = 852
Score = 249 bits (637), Expect = 3e-67
Identities = 125/163 (76%), Positives = 135/163 (82%)
Frame = +3
Query: 1 MLLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLI 60
MLL SPIS+ + HLN+ LR+ + LKI +MAKEGSDT + E AAIAGGLI
Sbjct: 45 MLLLSPISSCNVRSKHLNYSLRRRSCYSSPRALKIVSMAKEGSDTGNGVVEKAAIAGGLI 224
Query: 61 STPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGP 120
STPVI WSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIV WS+YTK KTGSGLPNGP
Sbjct: 225 STPVIAWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVGWSLYTKAKTGSGLPNGP 404
Query: 121 FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG 163
FGLLGAVEGL YLALVAIVVVF LQ+ +GYIPGPLPADQCFG
Sbjct: 405 FGLLGAVEGLYYLALVAIVVVFRLQFLDKGYIPGPLPADQCFG 533
>TC215177 homologue to GB|AAP31934.1|30387537|BT006590 At2g35860 {Arabidopsis
thaliana;} , partial (10%)
Length = 768
Score = 29.6 bits (65), Expect = 0.67
Identities = 13/43 (30%), Positives = 23/43 (53%)
Frame = -1
Query: 4 FSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTN 46
F+ I KTF++H ++ L + K H++ L + N + D N
Sbjct: 612 FTAILLHKTFSSHQHNLLTESKLHYILFSLDLRNFCSQVQDPN 484
>TC204630 similar to UP|Q8RWF0 (Q8RWF0) 26S proteasome subunit-like protein
(26S proteasome subunit RPN9a), partial (37%)
Length = 482
Score = 28.9 bits (63), Expect = 1.1
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 99 VGIVAWSIYTKTKTGSGLPN 118
+G + W IYT+T +G+ LP+
Sbjct: 112 IGTIRWRIYTRTSSGTSLPS 171
>AI988139
Length = 484
Score = 28.5 bits (62), Expect = 1.5
Identities = 26/80 (32%), Positives = 37/80 (45%), Gaps = 10/80 (12%)
Frame = -1
Query: 59 LISTPVI-----GWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAW-----SIYT 108
LIS PV+ GW LPP PG + + + +VGI+ SIY+
Sbjct: 361 LISVPVVFASXEGW------VHDXTLPPFPGRQ----DTLGLVFLVGILNSLIS*SSIYS 212
Query: 109 KTKTGSGLPNGPFGLLGAVE 128
++KT LP FGL G ++
Sbjct: 211 RSKTK*PLPQNSFGLSGTLK 152
>BU549976
Length = 735
Score = 28.5 bits (62), Expect = 1.5
Identities = 19/57 (33%), Positives = 25/57 (43%)
Frame = +3
Query: 75 TGCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGPFGLLGAVEGLS 131
T C L P I L +V WSI TG +GP G++G++EG S
Sbjct: 291 TPCSLVSVPSVMITILFFFFLFFLVIS*PWSIGVSVSTGG---DGPIGIIGSIEGAS 452
>TC230140 similar to UP|O03296 (O03296) Cytochrome b (Fragment), partial (5%)
Length = 1462
Score = 26.9 bits (58), Expect = 4.3
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = -1
Query: 7 ISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSD 44
++T T L HP + PK H++ K+ TNM K S+
Sbjct: 718 LNTKAQVTKLLYHP-KTPKCTHIKNKMNNTNMRKSRSN 608
>TC204995 homologue to UP|V0D2_ARATH (Q9LHA4) Probable vacuolar ATP synthase
subunit d 2 (V-ATPase d subunit 2) (Vacuolar proton
pump d subunit 2) , complete
Length = 1533
Score = 26.2 bits (56), Expect = 7.4
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +3
Query: 6 PISTPKTFTNHLNHPLRKPKSHHVR 30
PI + ++HL HP R P+ H R
Sbjct: 105 PIRNVRIRSSHLQHPRRLPRGHRPR 179
>BU547639
Length = 354
Score = 26.2 bits (56), Expect = 7.4
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -2
Query: 6 PISTPKTFTNHLNHPLRKPKSHH 28
P S K+ TN +NH +++ SHH
Sbjct: 269 PFSFSKSSTNQVNHYVQEMNSHH 201
>BU082637 homologue to GP|3204132|emb| extensin {Cicer arietinum}, partial
(46%)
Length = 398
Score = 26.2 bits (56), Expect = 7.4
Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
Frame = +1
Query: 2 LLFSPISTPKTFTNHLNHPL--RKPKSHHVRLKLKI 35
LLF + P TFTNHL+ L P H KLK+
Sbjct: 178 LLFPQLLLPSTFTNHLHLRLISMLPHLHLSTSKLKL 285
>TC204369 similar to GB|BAB09857.1|9759402|AB008268 phi-1-like protein
{Arabidopsis thaliana;} , partial (75%)
Length = 1276
Score = 25.8 bits (55), Expect = 9.6
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = -1
Query: 60 ISTPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVS 94
+ P W L+ L+T+GC P S G VS
Sbjct: 226 VMRPFRSWPLWYLRTSGCSSTSSPTVSRGKRSSVS 122
>TC231569 similar to UP|Q9LIC8 (Q9LIC8) Emb|CAA64867.1, partial (28%)
Length = 605
Score = 25.8 bits (55), Expect = 9.6
Identities = 14/56 (25%), Positives = 26/56 (46%)
Frame = -1
Query: 26 SHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPP 81
S+H R+K+ T+ + S ++ + ST + WS+ + T G+PP
Sbjct: 281 SNHCRIKINRTHRVRRYSMCMPPRSDDGSKLP*SSSTTLAAWSVSSKSTNATGIPP 114
>TC226993 homologue to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydratase
(GDP-D-mannose dehydratase) (GMD) , partial (94%)
Length = 1388
Score = 25.8 bits (55), Expect = 9.6
Identities = 18/53 (33%), Positives = 24/53 (44%), Gaps = 2/53 (3%)
Frame = -3
Query: 86 SIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGPFGL--LGAVEGLSYLALV 136
++ EGVS VV G+ + G+G+ G FGL G E L LV
Sbjct: 687 AVAGEEGVSLAVVHGVPVGGAFGGGVGGAGVEGGGFGLRRRGGAEHLGGAGLV 529
>TC230206 similar to GB|AAP68317.1|31711922|BT008878 At5g64430 {Arabidopsis
thaliana;} , partial (28%)
Length = 1305
Score = 25.8 bits (55), Expect = 9.6
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +2
Query: 6 PISTPKTFTNHLNHPLRKPKSHHVRLK 32
P S + HL H R+P+SH R++
Sbjct: 356 PASRRRPRRPHLRHQRRRPRSHDARIR 436
>CD415868
Length = 584
Score = 25.8 bits (55), Expect = 9.6
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = -1
Query: 49 PTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPPGPGGSIG 88
PT + A G ++ G S+ TL T G PPGPGG G
Sbjct: 461 PTPVIA-PGAPLALTERGDSVVTLMDTPGG-PPGPGGDFG 348
>BU760650 similar to GP|28851007|gb tRNA nucleotidyltransferase {Pseudomonas
syringae pv. tomato str. DC3000}, partial (37%)
Length = 458
Score = 25.8 bits (55), Expect = 9.6
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 60 ISTPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVS 94
+STP +Y L GC PG GG+ + G+S
Sbjct: 345 LSTPATFRGVYRLVRDGCAGTPGAGGADLSTGGLS 449
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.140 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,895,807
Number of Sequences: 63676
Number of extensions: 104793
Number of successful extensions: 664
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 656
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 662
length of query: 163
length of database: 12,639,632
effective HSP length: 90
effective length of query: 73
effective length of database: 6,908,792
effective search space: 504341816
effective search space used: 504341816
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC147178.12