BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.13 + phase: 0 /pseudo
(459 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM085622 similar to GP|11875201|db P0458A05.23 {Oryza sativa (ja... 154 2e-44
BI699556 155 3e-38
TC221816 weakly similar to UP|Q70G10 (Q70G10) SUMO protease, par... 79 3e-32
TC222716 103 1e-22
BU081914 67 2e-11
BI426728 39 0.004
TC226092 homologue to UP|ERF3_ARATH (O80339) Ethylene responsive... 34 0.16
TC226095 similar to UP|ERF3_ARATH (O80339) Ethylene responsive e... 34 0.16
TC226097 similar to UP|ERF3_ARATH (O80339) Ethylene responsive e... 33 0.27
CA935575 33 0.35
CA853663 weakly similar to PIR|A86318|A86 protein F15H18.11 [imp... 32 0.59
CK768433 31 1.3
TC204281 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 su... 31 1.3
TC206630 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, comp... 30 1.7
TC209187 30 1.7
TC215659 homologue to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragm... 30 1.7
TC206632 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, part... 30 1.7
TC226096 similar to UP|ERF3_ARATH (O80339) Ethylene responsive e... 30 1.7
TC231036 similar to UP|Q9LU42 (Q9LU42) Emb|CAB45082.1 (GATA-type... 30 2.3
TC206237 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (11%) 30 2.3
>BM085622 similar to GP|11875201|db P0458A05.23 {Oryza sativa (japonica
cultivar-group)}, partial (22%)
Length = 429
Score = 154 bits (389), Expect(2) = 2e-44
Identities = 76/98 (77%), Positives = 85/98 (86%), Gaps = 2/98 (2%)
Frame = +3
Query: 245 KKELVEE--VPREPFVPLTKEEEVEVTRAFSANQNKVLVNHEKSNIEISGKMFRCLRPGE 302
KKELVEE VP+EPFV LT EEE EV RAFSAN+ ++LV H+ SNIEI+G+ F+CLRP
Sbjct: 3 KKELVEEEEVPQEPFVALTSEEEDEVERAFSANRWRILVTHKNSNIEITGEKFQCLRPAG 182
Query: 303 WLNDEVINLYLELLKERERREPQKFLKCHFFNTFFYKK 340
WLNDEVINLYLELLKERE+REP KFLKCHFFNTFFYKK
Sbjct: 183 WLNDEVINLYLELLKEREQREPLKFLKCHFFNTFFYKK 296
Score = 43.1 bits (100), Expect(2) = 2e-44
Identities = 28/53 (52%), Positives = 31/53 (57%), Gaps = 4/53 (7%)
Frame = +2
Query: 332 FFNTFFYKK----QECL*LQISEEMDYSKEIGIWPT*M**NICTYSPGDPLVL 380
FF F +K E L* QI ++MD E GI T*M**NICTY D LVL
Sbjct: 269 FFQYLFLQKVNKWSERL*FQICQKMDKPTEFGIQST*M**NICTYPSRDTLVL 427
>BI699556
Length = 421
Score = 155 bits (393), Expect = 3e-38
Identities = 85/131 (64%), Positives = 101/131 (76%), Gaps = 11/131 (8%)
Frame = +3
Query: 174 GGIQQQSTSSMV-----------DAAKMLDNLSLSTKNDLSGVCAYKKLLEAVDKRGDTL 222
GG QQQSTSS+V +A KMLD LSL+ +DLS V AYKKLL+A+ +R DTL
Sbjct: 27 GGPQQQSTSSVVSELTNGDLNVVNAEKMLDILSLTPDHDLSSVQAYKKLLDALGQRTDTL 206
Query: 223 GRLKFEIQLNEKRKSAFDLLRPKKELVEEVPREPFVPLTKEEEVEVTRAFSANQNKVLVN 282
RL+ EI++NEKR S F+LLRPKKELVEEVP EPF+PLTKEEEVEV AFS N+ K+LV+
Sbjct: 207 NRLEAEIKVNEKRMSTFELLRPKKELVEEVPLEPFIPLTKEEEVEVAHAFSTNRKKILVS 386
Query: 283 HEKSNIEISGK 293
HEKS IEISG+
Sbjct: 387 HEKSGIEISGE 419
>TC221816 weakly similar to UP|Q70G10 (Q70G10) SUMO protease, partial (28%)
Length = 943
Score = 79.3 bits (194), Expect(2) = 3e-32
Identities = 45/61 (73%), Positives = 46/61 (74%)
Frame = +2
Query: 339 KKQECL*LQISEEMDYSKEIGIWPT*M**NICTYSPGDPLVLSCYQ*EGKEISVS*FTER 398
K QE +* QI EEMD KEIGIWPT*M**NICTY D LVL CYQ*E EISVS TER
Sbjct: 215 KWQEWV*FQICEEMDQPKEIGIWPT*M**NICTYP*RDSLVLGCYQ*ER*EISVSGLTER 394
Query: 399 N 399
N
Sbjct: 395 N 397
Score = 77.8 bits (190), Expect(2) = 3e-32
Identities = 42/73 (57%), Positives = 46/73 (62%), Gaps = 21/73 (28%)
Frame = +3
Query: 408 DEVKDKTGEDVDISSWETEFVEDLPEQMNG---------------------FVIEHMPYF 446
DEVKDKTG+D+D+SSW+ EFVEDLPEQ NG F EHM YF
Sbjct: 438 DEVKDKTGKDIDVSSWKKEFVEDLPEQQNGYDCGVFMIKYADFYSRNLGLCFNQEHMSYF 617
Query: 447 RLRTAKEILRLKA 459
R RTAKEILRLKA
Sbjct: 618 RRRTAKEILRLKA 656
>TC222716
Length = 650
Score = 103 bits (258), Expect = 1e-22
Identities = 66/168 (39%), Positives = 100/168 (59%), Gaps = 7/168 (4%)
Frame = +1
Query: 1 MAIITHLTGKRPHHWLSVDHNRPSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRISRYP 60
MA++T GKR HW S +++ PSPNS KR ++ST N+ L++ + + RIS+YP
Sbjct: 169 MAVVTS-NGKRRQHWPSANYHFPSPNS---KRPRVSTMSQNHAQSLLSSTSIISRISKYP 336
Query: 61 KTKPPLRREIHAPCSSRPRKFD---LSTNLKLTESSNLMASDTTDELMEKYEHAKHAALA 117
+TKPPL RE+HAPC RPRKFD + + + + D + L + Y+ AK++ALA
Sbjct: 337 ETKPPLHREVHAPC--RPRKFDSPAFNRSFHYPRKEEI-SYDKGNVLSKNYQRAKNSALA 507
Query: 118 TISFREKGK----VKDNIKEVVVLKDSNVEDVEEFLSVVKDHKLKENG 161
+I F EKGK V + + +V +DS+VE+ ++HK +E G
Sbjct: 508 SIRFGEKGKEVIEVDADSSKGMVSEDSSVEEDG------REHKWQEGG 633
>BU081914
Length = 421
Score = 67.0 bits (162), Expect = 2e-11
Identities = 33/55 (60%), Positives = 41/55 (74%)
Frame = -2
Query: 198 TKNDLSGVCAYKKLLEAVDKRGDTLGRLKFEIQLNEKRKSAFDLLRPKKELVEEV 252
T+ DL V YKKLLE V +R DTL RL FEI L+EKR+ ++L+RPKKELVE +
Sbjct: 324 TQRDLENVHVYKKLLEDVGRRSDTLQRLNFEIDLHEKRRDYYNLVRPKKELVEVI 160
>BI426728
Length = 404
Score = 39.3 bits (90), Expect = 0.004
Identities = 18/55 (32%), Positives = 33/55 (59%)
Frame = +1
Query: 284 EKSNIEISGKMFRCLRPGEWLNDEVINLYLELLKERERREPQKFLKCHFFNTFFY 338
E + IS + L+P ++ND +I+ Y++ LK++ + Q + HFFN+FF+
Sbjct: 244 EPDAVSISKRDIELLQPQTFINDTIIDFYIKYLKKKLPTDEQN--RFHFFNSFFF 402
>TC226092 homologue to UP|ERF3_ARATH (O80339) Ethylene responsive element
binding factor 3 (AtERF3), partial (48%)
Length = 884
Score = 33.9 bits (76), Expect = 0.16
Identities = 20/77 (25%), Positives = 30/77 (37%), Gaps = 8/77 (10%)
Frame = +3
Query: 8 TGKRPHHWLSVDHNRPSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRIS--------RY 59
TG H ++N +P PT + + PP TT T + RY
Sbjct: 561 TGFHDQHHHHNNNNLNNPQRPTSSGMSSTVESFSGPRPPTTTTTTTITTATPFLTATRRY 740
Query: 60 PKTKPPLRREIHAPCSS 76
P+T P + + H+ C S
Sbjct: 741 PRTPPLVPEDCHSDCDS 791
>TC226095 similar to UP|ERF3_ARATH (O80339) Ethylene responsive element
binding factor 3 (AtERF3), partial (28%)
Length = 587
Score = 33.9 bits (76), Expect = 0.16
Identities = 20/77 (25%), Positives = 30/77 (37%), Gaps = 8/77 (10%)
Frame = +3
Query: 8 TGKRPHHWLSVDHNRPSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRIS--------RY 59
TG H ++N +P PT + + PP TT T + RY
Sbjct: 75 TGFHDQHHHHNNNNLNNPQRPTSSGMSSTVESFSGPRPPTTTTTTTITTATPFLTATRRY 254
Query: 60 PKTKPPLRREIHAPCSS 76
P+T P + + H+ C S
Sbjct: 255 PRTPPLVPEDCHSDCDS 305
>TC226097 similar to UP|ERF3_ARATH (O80339) Ethylene responsive element
binding factor 3 (AtERF3), partial (36%)
Length = 466
Score = 33.1 bits (74), Expect = 0.27
Identities = 18/60 (30%), Positives = 25/60 (41%), Gaps = 4/60 (6%)
Frame = +2
Query: 21 NRPSPNSPTFKRQKLSTTMSNNTPPPLTTNAT----VFRISRYPKTKPPLRREIHAPCSS 76
N +P PT + N PP TT T + RYP+T P + + H+ C S
Sbjct: 194 NLNNPQRPTSSGMSSTVESFNGPRPPTTTTTTTTPFLTTTRRYPRTPPLVPEDCHSDCDS 373
>CA935575
Length = 410
Score = 32.7 bits (73), Expect = 0.35
Identities = 36/136 (26%), Positives = 63/136 (45%), Gaps = 10/136 (7%)
Frame = +1
Query: 79 RKFDLSTNLKLTESSNLMASDTTDELMEKYEHAKHAALATISFREKGKVKDNIKEVVVLK 138
R+ L LKLTE N + + L+ + + A A E+G++K+N+KEV
Sbjct: 1 REHHLVNCLKLTEEENSSLGNEINRLIRLLKETEEEANA--KREEEGQLKENLKEV---- 162
Query: 139 DSNVEDVEEFLSVVK--DHKLKENGLVCLDDKVEVVNGGIQQQ--------STSSMVDAA 188
++ V ++E L K KLKE+ LD + E N + + S + + +
Sbjct: 163 EAEVIHLQEELKEAKAESMKLKES---LLDKENEFQNIFEENEELRLRESTSIKKVEELS 333
Query: 189 KMLDNLSLSTKNDLSG 204
KMLD ++ + + +G
Sbjct: 334 KMLDEVTSRNQTEENG 381
>CA853663 weakly similar to PIR|A86318|A86 protein F15H18.11 [imported] -
Arabidopsis thaliana, partial (4%)
Length = 435
Score = 32.0 bits (71), Expect = 0.59
Identities = 24/70 (34%), Positives = 32/70 (45%)
Frame = +1
Query: 17 SVDHNRPSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRISRYPKTKPPLRREIHAPCSS 76
S H P N P F Q+L T+ P L T F +P +KP R++H P S
Sbjct: 244 SDSHVHPIHNPPNFLPQQLPPTLQ----PHLFTTKRFFLY--WPHSKPH-GRKVHIPTGS 402
Query: 77 RPRKFDLSTN 86
+P F L+ N
Sbjct: 403 KPDIFRLAPN 432
>CK768433
Length = 619
Score = 30.8 bits (68), Expect = 1.3
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +2
Query: 189 KMLDNLSLSTKNDLSGVCAYKKLLEAVDKRGDTLGRLKFEIQLNEKRKSAFDLLRPKKEL 248
K D+ +L N L A+K LLE+V+ + D +N+KR +A + L +K+
Sbjct: 338 KTNDDETLVYANKLEAKLAFKALLESVNVQSDWTWEQAMREIINDKRYNALNTLGERKQA 517
Query: 249 VEE 251
E
Sbjct: 518 FNE 526
>TC204281 similar to UP|Q8H1Y2 (Q8H1Y2) 20S proteasome alpha 6 subunit,
partial (91%)
Length = 1275
Score = 30.8 bits (68), Expect = 1.3
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +1
Query: 15 WLSVDHNRPSPNSPTFKRQKLSTTMSNNTP----PPLTTNATVFRISRYPKTKPPLRREI 70
W S RP+PNSP +R+ +T+++ +P PP+ ++ P T P I
Sbjct: 331 WSSHASTRPTPNSPRTRRRSSRSTITSASPSLASPPMAASS--------PAT*DPNASTI 486
Query: 71 HAPCSSRPRKFD 82
P S R D
Sbjct: 487 ITPTSRRSPSAD 522
>TC206630 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, complete
Length = 892
Score = 30.4 bits (67), Expect = 1.7
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 321 RREPQKFLKCHFFNTFFYKKQECL*LQISEEMDYSKEI 358
R P F FFN +FYKK++C + D+ +E+
Sbjct: 377 RPSP*SFFLSFFFNYYFYKKKKCFFFNEQDFCDFMQEM 490
>TC209187
Length = 1004
Score = 30.4 bits (67), Expect = 1.7
Identities = 17/57 (29%), Positives = 28/57 (48%)
Frame = -3
Query: 17 SVDHNRPSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRISRYPKTKPPLRREIHAP 73
S H P P SP+ ++ +T N+ PPP ++ T +++P PP H+P
Sbjct: 294 SYSHTAP-PKSPSPPPRRPATPPPNSPPPPPQSSTTPPEHAQHPPLHPPYPPLHHSP 127
>TC215659 homologue to UP|Q94FX1 (Q94FX1) Heme oxygenase 1 (Fragment),
complete
Length = 1365
Score = 30.4 bits (67), Expect = 1.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +1
Query: 312 YLELLKERERREPQKFLKCHFFNTFF 337
Y + LKE ++PQ F+ CHF+N +F
Sbjct: 925 YAQYLKELSVKDPQAFI-CHFYNIYF 999
>TC206632 homologue to UP|Q8H2B1 (Q8H2B1) DnaJ-like protein, partial (88%)
Length = 947
Score = 30.4 bits (67), Expect = 1.7
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 321 RREPQKFLKCHFFNTFFYKKQECL*LQISEEMDYSKEI 358
R P F FFN +FYKK++C + D+ +E+
Sbjct: 491 RPSP*SFFLSFFFNYYFYKKKKCFFFNEQDFCDFMQEM 604
>TC226096 similar to UP|ERF3_ARATH (O80339) Ethylene responsive element
binding factor 3 (AtERF3), partial (28%)
Length = 672
Score = 30.4 bits (67), Expect = 1.7
Identities = 16/57 (28%), Positives = 24/57 (42%), Gaps = 4/57 (7%)
Frame = +3
Query: 24 SPNSPTFKRQKLSTTMSNNTPPPLTTNAT----VFRISRYPKTKPPLRREIHAPCSS 76
+P PT + + PP TT T + RYP+T P + + H+ C S
Sbjct: 174 NPQRPTSSGMSSTVESFSGPRPPTTTTTTTTPFLTTTRRYPRTPPLVPEDCHSDCDS 344
>TC231036 similar to UP|Q9LU42 (Q9LU42) Emb|CAB45082.1 (GATA-type zinc finger
protein), partial (12%)
Length = 637
Score = 30.0 bits (66), Expect = 2.3
Identities = 17/43 (39%), Positives = 22/43 (50%)
Frame = +1
Query: 23 PSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRISRYPKTKPP 65
P+ SPT + T PPP+TT+ TV S+ KT PP
Sbjct: 211 PTAPSPTTTSITVPTPSKTTAPPPITTSITVPTPSK--KTAPP 333
>TC206237 similar to UP|Q9FMX6 (Q9FMX6) Gb|AAD21732.1, partial (11%)
Length = 607
Score = 30.0 bits (66), Expect = 2.3
Identities = 24/74 (32%), Positives = 31/74 (41%)
Frame = +1
Query: 23 PSPNSPTFKRQKLSTTMSNNTPPPLTTNATVFRISRYPKTKPPLRREIHAPCSSRPRKFD 82
P P +P S +S+ PP TT + I+ PKT P P S P F
Sbjct: 307 PVPRTPP------SRFLSSALSPPPTTRSRSPAIAPSPKTSSPAAGRFSPPFSLTPPSFA 468
Query: 83 LSTNLKLTESSNLM 96
S + +L SS LM
Sbjct: 469 *SFDTQLFISSPLM 510
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.138 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,774,551
Number of Sequences: 63676
Number of extensions: 262003
Number of successful extensions: 2361
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 2308
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2346
length of query: 459
length of database: 12,639,632
effective HSP length: 101
effective length of query: 358
effective length of database: 6,208,356
effective search space: 2222591448
effective search space used: 2222591448
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 61 (28.1 bits)
Medicago: description of AC147012.13