BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.12 + phase: 0
(750 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC230638 144 1e-34
TC230637 89 5e-18
BM309814 similar to PIR|E86366|E86 protein F26F24.6 [imported] -... 31 1.8
TC219786 similar to GB|AAM91354.1|22137018|AY133524 At2g28390/T1... 31 1.8
TC217545 similar to UP|Q9ZUI6 (Q9ZUI6) T2K10.12 protein, partial... 31 1.8
TC204106 similar to UP|PSAD_NICSY (P29302) Photosystem I reactio... 31 1.8
TC224492 similar to UP|Q9VJD4 (Q9VJD4) CG5094-PA (LD24721p), par... 31 1.8
TC216052 weakly similar to UP|Q43486 (Q43486) B32E protein, part... 31 2.3
BF596789 similar to GP|20147343|gb| AT4g15410/dl3750w {Arabidops... 30 3.9
TC204099 homologue to UP|PSAD_LYCES (P12372) Photosystem I react... 30 3.9
BG550814 similar to SP|P32869|PSAD Photosystem I reaction center... 30 3.9
TC210593 weakly similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1 (AT3... 30 3.9
AW203350 30 5.1
TC204920 similar to UP|PPF1_PEA (Q9FY06) Inner membrane protein ... 30 5.1
TC204052 homologue to UP|Q39835 (Q39835) Extensin, partial (67%) 30 5.1
TC214632 homologue to UP|Q9LKH8 (Q9LKH8) NADPH-protochlorophylli... 30 5.1
TC210486 weakly similar to UP|Q9LMD3 (Q9LMD3) F14D16.9, partial ... 30 5.1
TC218843 30 5.1
CF922312 29 6.7
TC208232 weakly similar to UP|Q84VQ5 (Q84VQ5) Hydrolase, alpha/b... 29 6.7
>TC230638
Length = 460
Score = 144 bits (363), Expect = 1e-34
Identities = 71/73 (97%), Positives = 71/73 (97%)
Frame = +1
Query: 678 HMYVDSREKVTARYQLTCPSKREMLVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQ 737
HMYVDSREKVTARYQLT PSKREM VFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQ
Sbjct: 10 HMYVDSREKVTARYQLTVPSKREMTVFGSFKQTGTLLPKAPKGWGWRTALLFDELADLLQ 189
Query: 738 NGALRVIAVVQLV 750
NGALRVIAVVQLV
Sbjct: 190 NGALRVIAVVQLV 228
>TC230637
Length = 421
Score = 89.4 bits (220), Expect = 5e-18
Identities = 43/43 (100%), Positives = 43/43 (100%)
Frame = +1
Query: 708 KQTGTLLPKAPKGWGWRTALLFDELADLLQNGALRVIAVVQLV 750
KQTGTLLPKAPKGWGWRTALLFDELADLLQNGALRVIAVVQLV
Sbjct: 1 KQTGTLLPKAPKGWGWRTALLFDELADLLQNGALRVIAVVQLV 129
>BM309814 similar to PIR|E86366|E86 protein F26F24.6 [imported] -
Arabidopsis thaliana, partial (22%)
Length = 398
Score = 31.2 bits (69), Expect = 1.8
Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
Frame = +2
Query: 6 YPNNPPPS-PYNQRSASIPKQPPSHHS 31
+P + PPS P N+R +P++PP HS
Sbjct: 59 FPRHAPPSAPLNRRRPQLPRRPPQKHS 139
>TC219786 similar to GB|AAM91354.1|22137018|AY133524 At2g28390/T1B3.9
{Arabidopsis thaliana;} , partial (32%)
Length = 848
Score = 31.2 bits (69), Expect = 1.8
Identities = 24/89 (26%), Positives = 36/89 (39%)
Frame = +2
Query: 181 DSNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTGVGI 240
DS+ ++L +SP+L S P ++ S SDP +S Y G+ T T V
Sbjct: 65 DSDPPIRLHLPPLSPSLFDSEPPELSHGSSARPSSDPSSPTSSGYAAERGGSSTATSV-- 238
Query: 241 EGPSEEGSCYHSDNNNRLVRDQSRDSFSS 269
S+ H + + D S SS
Sbjct: 239 ---SQVDDVLHHEIQEITIHDSQPSSHSS 316
>TC217545 similar to UP|Q9ZUI6 (Q9ZUI6) T2K10.12 protein, partial (36%)
Length = 1597
Score = 31.2 bits (69), Expect = 1.8
Identities = 48/177 (27%), Positives = 69/177 (38%), Gaps = 23/177 (12%)
Frame = +2
Query: 425 RNGSFRPVRVHVRGAIDGLAGIGRGTTFVPAAASPPTRFVFSRVPFGVGNRNYP----QS 480
++ + R V +D IGR +P AAS PT F+ +P G P
Sbjct: 251 KHQNIRSTLVERMPVLDEATSIGRRAGSLPGAASTPTAPSFN-LPNGTAKPVAPLVDLLD 427
Query: 481 AANDDSEARADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQEMGLQSTAGGASTG 540
++DD+ A + +G GD L L+G+ +L+ Q QS AG AS
Sbjct: 428 LSSDDAPAPSSSSG---GDILQDLLGV------------DLSPASQ----QSVAGQASKS 550
Query: 541 GIPVQL---------LETPEHTIGIEWEN----------DNSSSISLDLKTPLSHFP 578
G V L +E+ T+ I N D SS+SL KT + P
Sbjct: 551 GNDVLLDLLSIGSPSVESSSSTVDILSSNSSNKAPVSSLDGLSSLSLSTKTTSNAAP 721
>TC204106 similar to UP|PSAD_NICSY (P29302) Photosystem I reaction center
subunit II, chloroplast precursor (Photosystem I 20 kDa
subunit) (PSI-D), partial (74%)
Length = 536
Score = 31.2 bits (69), Expect = 1.8
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +2
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHH 30
E P++ NNPPPSP RS S ++ H
Sbjct: 83 EPPRHGNNPPPSPSPPRSPSSSREQSEQH 169
>TC224492 similar to UP|Q9VJD4 (Q9VJD4) CG5094-PA (LD24721p), partial (6%)
Length = 525
Score = 31.2 bits (69), Expect = 1.8
Identities = 20/51 (39%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +1
Query: 478 PQSAANDDSEA-RADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQE 527
P +AANDDS+ + DL+G+G + G+ + N HTE Q EQE
Sbjct: 184 PSNAANDDSDGFETASDTDLAGEGDDSGAGIPEEQNRTEQSHTE-KQTEQE 333
>TC216052 weakly similar to UP|Q43486 (Q43486) B32E protein, partial (36%)
Length = 787
Score = 30.8 bits (68), Expect = 2.3
Identities = 15/40 (37%), Positives = 21/40 (52%)
Frame = +2
Query: 10 PPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLA 49
PPPS YNQR +Q SH S ++ ++ L NL+
Sbjct: 371 PPPSKYNQRQQFFEQQGASHSSSGSNSSSDSLVGQTQNLS 490
>BF596789 similar to GP|20147343|gb| AT4g15410/dl3750w {Arabidopsis
thaliana}, partial (15%)
Length = 421
Score = 30.0 bits (66), Expect = 3.9
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +2
Query: 241 EGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSCS 273
EG +G +S +NN L++ S S SSSSCS
Sbjct: 68 EGGFNDGFSLNSSSNNTLLKSNSVSSKSSSSCS 166
>TC204099 homologue to UP|PSAD_LYCES (P12372) Photosystem I reaction center
subunit II, chloroplast precursor (Photosystem I 20 kDa
subunit) (PSI-D), partial (80%)
Length = 953
Score = 30.0 bits (66), Expect = 3.9
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = +3
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRAL 44
E P++ NNPPPSP RS S ++ S+ PT + L
Sbjct: 192 EPPRHGNNPPPSPSPPRSPSSSRE----QSEQQPPTRPQRHRL 308
>BG550814 similar to SP|P32869|PSAD Photosystem I reaction center subunit
II chloroplast precursor (Photosystem I 20 kDa
subunit), partial (48%)
Length = 365
Score = 30.0 bits (66), Expect = 3.9
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = +1
Query: 2 ENPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRAL 44
E P++ NNPPPSP RS S ++ S+ PT + L
Sbjct: 4 EPPRHGNNPPPSPSPPRSPSSSRE----QSEQQPPTRPQRHRL 120
>TC210593 weakly similar to UP|Q9LJ86 (Q9LJ86) Gb|AAD48513.1
(AT3g22600/F16J14_17), partial (56%)
Length = 728
Score = 30.0 bits (66), Expect = 3.9
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 1/51 (1%)
Frame = +2
Query: 199 PSYPSDME-GPSLGNSLSDPDGVRTSCYVEVPLGAGTTTGVGIEGPSEEGS 248
P+ PS G G+S P G T + P GAG+ T I+G S +G+
Sbjct: 434 PTTPSTAPVGSPSGSSAESPQGSITPSASDFPSGAGSKTVPSIDGGSSDGN 586
>AW203350
Length = 411
Score = 29.6 bits (65), Expect = 5.1
Identities = 16/43 (37%), Positives = 25/43 (57%)
Frame = +2
Query: 8 NNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLAS 50
++P P P + RS+ P+Q P+ H PTTA + + +LAS
Sbjct: 143 SSPDPPPKSPRSS--PEQNPNSHRSFFSPTTASVTSSAISLAS 265
>TC204920 similar to UP|PPF1_PEA (Q9FY06) Inner membrane protein PPF-1,
chloroplast precursor (Post-floral-specific protein 1),
partial (77%)
Length = 1245
Score = 29.6 bits (65), Expect = 5.1
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +3
Query: 4 PQYPNNPPPSPYNQRSASIPKQPP 27
P P+ PPP P+ R P+QPP
Sbjct: 111 PLTPSPPPPPPHQARRHQSPRQPP 182
>TC204052 homologue to UP|Q39835 (Q39835) Extensin, partial (67%)
Length = 1083
Score = 29.6 bits (65), Expect = 5.1
Identities = 17/48 (35%), Positives = 20/48 (41%)
Frame = +3
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLAS 50
+P P + PP PY S PK P PTT RAL + S
Sbjct: 609 SPPPPKHSPPPPYYYHSPPPPKHSPRSSLLLQVPTTTT*RALQIPITS 752
>TC214632 homologue to UP|Q9LKH8 (Q9LKH8) NADPH-protochlorophyllide
oxidoreductase, complete
Length = 1525
Score = 29.6 bits (65), Expect = 5.1
Identities = 19/42 (45%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Frame = -1
Query: 138 CTNKMLETDLGKMSLKSDLK--DTSMPNLLVELGDSEGDFNS 177
C N +L+ D G+ LKSDL+ D PN++V L D+ DF S
Sbjct: 220 CWNSLLQVDEGE--LKSDLRVSDNETPNIVVSLRDTP-DFPS 104
>TC210486 weakly similar to UP|Q9LMD3 (Q9LMD3) F14D16.9, partial (45%)
Length = 827
Score = 29.6 bits (65), Expect = 5.1
Identities = 15/32 (46%), Positives = 19/32 (58%)
Frame = +1
Query: 8 NNPPPSPYNQRSASIPKQPPSHHSDNNDPTTA 39
N PPS QRS+ P PP+H SD+ + T A
Sbjct: 220 NGSPPSVPGQRSSPEP-NPPNHVSDSKEETAA 312
>TC218843
Length = 810
Score = 29.6 bits (65), Expect = 5.1
Identities = 19/62 (30%), Positives = 27/62 (42%)
Frame = +2
Query: 3 NPQYPNNPPPSPYNQRSASIPKQPPSHHSDNNDPTTAELRALDCNLASLCEHVQIEGFNS 62
+P ++PPPSP RS P P S +S N + + A L ++ FN
Sbjct: 128 SPSSSSSPPPSPLKPRSVVSPISPISRYSAVNMNPHHGVVSYKDMFAELVNSLEGLSFNE 307
Query: 63 GS 64
GS
Sbjct: 308 GS 313
>CF922312
Length = 779
Score = 29.3 bits (64), Expect = 6.7
Identities = 26/106 (24%), Positives = 40/106 (37%), Gaps = 2/106 (1%)
Frame = -2
Query: 536 GASTGGIPVQLLETPEHTIGIEWENDNSSSISLDLKTPLS--HFPPFRFGVSFEEVHRLG 593
G S+ + V L++ +H + + L PL HFP +F SF +HR
Sbjct: 400 GGSSHRVFVMFLQSKQHI--------HPGPVPLGHTFPLGIVHFPEQKFEASFMSIHRPS 245
Query: 594 DGQVKHSPEVFYAGSLWKVSIQAFNDEDPQGRRTLGNVFITSKLSP 639
G S WK I + + E N+ + +KL P
Sbjct: 244 TGT-----------SSWKRRISSTHQETVLD*ENQENILLLAKLEP 140
>TC208232 weakly similar to UP|Q84VQ5 (Q84VQ5) Hydrolase, alpha/beta fold
family, partial (28%)
Length = 417
Score = 29.3 bits (64), Expect = 6.7
Identities = 17/42 (40%), Positives = 21/42 (49%), Gaps = 2/42 (4%)
Frame = -2
Query: 6 YPNNPPPSPYNQRSASIPKQPPSHH--SDNNDPTTAELRALD 45
+PN PPSP+N QPP + S P T LR+LD
Sbjct: 404 WPNCKPPSPHNWSPPRETSQPPIYPKVSPATLPETFRLRSLD 279
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.316 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,255,113
Number of Sequences: 63676
Number of extensions: 570914
Number of successful extensions: 4617
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 3941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4542
length of query: 750
length of database: 12,639,632
effective HSP length: 104
effective length of query: 646
effective length of database: 6,017,328
effective search space: 3887193888
effective search space used: 3887193888
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC147012.12