BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.5 - phase: 0
(221 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229716 186 5e-48
TC229717 164 2e-41
CO984637 126 5e-37
TC225444 homologue to UP|RL24_CICAR (O65743) 60S ribosomal prote... 31 0.38
TC225446 similar to UP|RL24_HORVU (P50888) 60S ribosomal protein... 31 0.50
TC228085 similar to UP|Q9LK27 (Q9LK27) Gb|AAF01563.1, partial (17%) 30 0.86
TC224859 weakly similar to UP|Q7TP79 (Q7TP79) Aa2-245, partial (3%) 29 1.5
CF805725 29 1.5
TC209515 similar to UP|Q8VWH9 (Q8VWH9) Stress responsive gene 6,... 29 1.5
TC216353 29 1.9
TC206426 similar to UP|Q9FZE9 (Q9FZE9) T2E6.21, partial (12%) 29 1.9
CF922531 29 1.9
TC232829 similar to UP|SYN2_HUMAN (Q92777) Synapsin II, partial ... 28 2.5
AI900422 28 2.5
TC225443 homologue to UP|RL24_CICAR (O65743) 60S ribosomal prote... 28 2.5
TC209388 similar to UP|Q94T09 (Q94T09) NADH dehydrogenase subuni... 28 2.5
TC223418 similar to UP|Q941B8 (Q941B8) At2g13860/F13J11.19, part... 28 3.3
TC215643 similar to UP|Q84MC1 (Q84MC1) At4g31290, partial (80%) 28 4.2
TC224563 homologue to UP|RL15_ARATH (O23515) 60S ribosomal prote... 27 5.5
BE803455 similar to GP|4379369|emb| Calmodulin {Physcomitrella p... 27 7.2
>TC229716
Length = 839
Score = 186 bits (473), Expect = 5e-48
Identities = 89/147 (60%), Positives = 116/147 (78%)
Frame = +3
Query: 1 MAEEELNNGYCSSSGDEDGDAAWKAAIDSVAGTSSYVTSFMNGFSATNNNDTKKKHNDNQ 60
M E E NGYCSS G+EDGDAAW+AAIDS+A T++YV S + +N+ +D+
Sbjct: 36 MPERESTNGYCSS-GEEDGDAAWRAAIDSIAQTTTYVAS-----TTKHNHPNHHIDDDDH 197
Query: 61 NPKSPKIKHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIRLFRHSKPGIVFD 120
P + ++KHYQLKA KLL++ILEN+IEIV++P+PV+DEDP ++CGIRLF+H++PGIVFD
Sbjct: 198 KPNTQQLKHYQLKAQKLLNEILENSIEIVRKPVPVVDEDPESNECGIRLFKHAQPGIVFD 377
Query: 121 HADEPQPPMKRPKLVPGEDIDEKSKKF 147
H DE QPP KRP+L+PG DIDEKSKKF
Sbjct: 378 HVDELQPPRKRPRLLPGGDIDEKSKKF 458
Score = 124 bits (310), Expect = 4e-29
Identities = 62/81 (76%), Positives = 72/81 (88%)
Frame = +1
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
E + + FR++IRS+AV+G D+IAAANDAYKKSLARLEAKDAAAKAKAKREEERIE L
Sbjct: 430 EILMRNQRSFRKQIRSVAVNGIDIIAAANDAYKKSLARLEAKDAAAKAKAKREEERIENL 609
Query: 198 KKIRGERWLPSMAKEMQAKIK 218
KKIRGE+WLPSMA+EMQ KI+
Sbjct: 610 KKIRGEKWLPSMAQEMQVKIR 672
>TC229717
Length = 427
Score = 164 bits (416), Expect = 2e-41
Identities = 80/133 (60%), Positives = 102/133 (76%), Gaps = 1/133 (0%)
Frame = +2
Query: 1 MAEEELNNGYCSSS-GDEDGDAAWKAAIDSVAGTSSYVTSFMNGFSATNNNDTKKKHNDN 59
M E E NGYCSSS G+EDGDAAW+AAIDS+A T++YV+S T+ N D
Sbjct: 38 MPEGEPTNGYCSSSSGEEDGDAAWRAAIDSIAQTTTYVSSTTKH---THPNHDDDDDYDG 208
Query: 60 QNPKSPKIKHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIRLFRHSKPGIVF 119
PK+ ++KHYQLKA LL++ILEN+IEIV++P+PV+DEDP ++CGIRLF+H++PGIVF
Sbjct: 209 HKPKTRQLKHYQLKAQNLLNEILENSIEIVRKPVPVVDEDPESNECGIRLFKHAQPGIVF 388
Query: 120 DHADEPQPPMKRP 132
DH DEPQPP KRP
Sbjct: 389 DHVDEPQPPRKRP 427
>CO984637
Length = 778
Score = 126 bits (317), Expect(2) = 5e-37
Identities = 64/77 (83%), Positives = 72/77 (93%)
Frame = -2
Query: 145 KKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKLKKIRGER 204
++FR++IRS+AV+G D+IAAANDAYKKSLARLEAKDAAAKAKAKREEERIE LKKIRGER
Sbjct: 411 QQFRKQIRSVAVNGIDIIAAANDAYKKSLARLEAKDAAAKAKAKREEERIENLKKIRGER 232
Query: 205 WLPSMAKEMQAKIKIKH 221
WLPSMAKEMQ KI+ H
Sbjct: 231 WLPSMAKEMQVKIRH*H 181
Score = 45.1 bits (105), Expect(2) = 5e-37
Identities = 20/25 (80%), Positives = 22/25 (88%)
Frame = -3
Query: 122 ADEPQPPMKRPKLVPGEDIDEKSKK 146
ADE QPP KRP+L+PG DIDEKSKK
Sbjct: 590 ADELQPPRKRPRLLPGGDIDEKSKK 516
>TC225444 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protein L24,
complete
Length = 750
Score = 31.2 bits (69), Expect = 0.38
Identities = 22/81 (27%), Positives = 40/81 (49%)
Frame = +2
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
+DI +++ K RRR + + A KK + E +DAA +A+ + +ERI+K
Sbjct: 230 KDIAQEAVKKRRRATKKPYSRSIVGATLEVIQKKRTEKPEVRDAAREAQLREIKERIKKT 409
Query: 198 KKIRGERWLPSMAKEMQAKIK 218
K + + AK +++ K
Sbjct: 410 KDDKKAKKAEVAAKSQKSQGK 472
>TC225446 similar to UP|RL24_HORVU (P50888) 60S ribosomal protein L24,
partial (96%)
Length = 865
Score = 30.8 bits (68), Expect = 0.50
Identities = 22/78 (28%), Positives = 37/78 (47%)
Frame = +2
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
+DI +++ K RRR + + A KK + E +DAA +A + +ERI+K
Sbjct: 281 KDIAQEAVKKRRRAAKKPYSRSIVGATLEVIQKKRAEKPEVRDAAREAALREIKERIKKT 460
Query: 198 KKIRGERWLPSMAKEMQA 215
K + + AK +A
Sbjct: 461 KDEKKAKKAEVTAKSQKA 514
>TC228085 similar to UP|Q9LK27 (Q9LK27) Gb|AAF01563.1, partial (17%)
Length = 947
Score = 30.0 bits (66), Expect = 0.86
Identities = 24/71 (33%), Positives = 39/71 (54%)
Frame = +1
Query: 123 DEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAA 182
DE + P K + ED++ + K+ + R+++ A AA +A +K+ A +AA
Sbjct: 10 DEKRDPEKLR--IEREDLERRQKEEKARLQAEA-------KAAEEAQRKAEA-----EAA 147
Query: 183 AKAKAKREEER 193
A+AK KRE ER
Sbjct: 148 AEAKRKRELER 180
>TC224859 weakly similar to UP|Q7TP79 (Q7TP79) Aa2-245, partial (3%)
Length = 843
Score = 29.3 bits (64), Expect = 1.5
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = +2
Query: 29 SVAGTSSYVTSFMNGFSATNNNDTKKKHNDNQNPKSPKIK 68
S+ G + S +G + NNND K +N+NQ P+S K
Sbjct: 2 SIEGFHHQIISLFSGNN--NNNDNKNNNNNNQYPQSALYK 115
>CF805725
Length = 571
Score = 29.3 bits (64), Expect = 1.5
Identities = 23/100 (23%), Positives = 40/100 (40%), Gaps = 5/100 (5%)
Frame = +3
Query: 56 HNDNQNPKSPKIKHYQLKALKL-----LDDILENTIEIVKEPIPVLDEDPNVDDCGIRLF 110
H K P I H + + + L + +++ + + P P P D C RL
Sbjct: 3 HEHRHTSKYPIISHGKCHSSRCGGSAQLSFSVGDSLRLRRHPGPSPCSPPRRDRCQGRLL 182
Query: 111 RHSKPGIVFDHADEPQPPMKRPKLVPGEDIDEKSKKFRRR 150
R + + D P+PP +R + P + ++ RRR
Sbjct: 183 RSRRLQL-----DAPRPPHRRAESQPRRLLQRAQRRLRRR 287
>TC209515 similar to UP|Q8VWH9 (Q8VWH9) Stress responsive gene 6, Srg6
(Stress responsive gene 6 protein, Srg6), partial (55%)
Length = 1386
Score = 29.3 bits (64), Expect = 1.5
Identities = 29/114 (25%), Positives = 49/114 (42%), Gaps = 10/114 (8%)
Frame = +2
Query: 91 EPIPVLDEDPNVDDCGIRLFRHSK---PGIVFDHADEPQPPMKRPKLVPGEDIDE----- 142
EP+P +EDPN +D R+ + + PG + + + + + P E+ DE
Sbjct: 173 EPVPEEEEDPNKNDDDERMHKKKRLIFPGKTLAKKKKKKKTISKLESSPKENEDEEHSGK 352
Query: 143 --KSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERI 194
+ + R IR + A DA + A L+A K K + EE+R+
Sbjct: 353 VVEDEGGERMIRKSTRTSVIVRQAERDAIR---AALQATIKPVKRKKEGEEKRM 505
>TC216353
Length = 2055
Score = 28.9 bits (63), Expect = 1.9
Identities = 18/68 (26%), Positives = 30/68 (43%)
Frame = +1
Query: 24 KAAIDSVAGTSSYVTSFMNGFSATNNNDTKKKHNDNQNPKSPKIKHYQLKALKLLDDILE 83
+AA+D +A Y + + G+ T D +DN NPK Y K+L L+
Sbjct: 1426 RAALDQLA---DYFKARLAGYPTTLAEDESMLTDDNLNPKKRVATQYVRLEKKMLHACLQ 1596
Query: 84 NTIEIVKE 91
T + + +
Sbjct: 1597 ATTDFINQ 1620
>TC206426 similar to UP|Q9FZE9 (Q9FZE9) T2E6.21, partial (12%)
Length = 1035
Score = 28.9 bits (63), Expect = 1.9
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 3/68 (4%)
Frame = +1
Query: 5 ELNNGYCSSSGDEDGDAAWKAA---IDSVAGTSSYVTSFMNGFSATNNNDTKKKHNDNQN 61
E N SSS ++ D A + + + A ++ V N F A N + N+ +N
Sbjct: 529 ERNFSSVSSSQGQEFDFAMAGSGRKVSNAANSTGSVVETWNAFFA-NPGEWWDNRNNKRN 705
Query: 62 PKSPKIKH 69
PK+P KH
Sbjct: 706 PKAPDFKH 729
>CF922531
Length = 602
Score = 28.9 bits (63), Expect = 1.9
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = -2
Query: 178 AKDAAAKAKAKREEERIEKLKKIRGERWLPSMAKEMQAKIK 218
AKD + EEE++EK KK + + L S AK + K K
Sbjct: 538 AKDQLTMKDKRDEEEKLEKQKKKKDSKALSSKAKGKEKKEK 416
>TC232829 similar to UP|SYN2_HUMAN (Q92777) Synapsin II, partial (3%)
Length = 425
Score = 28.5 bits (62), Expect = 2.5
Identities = 15/62 (24%), Positives = 30/62 (48%)
Frame = -3
Query: 81 ILENTIEIVKEPIPVLDEDPNVDDCGIRLFRHSKPGIVFDHADEPQPPMKRPKLVPGEDI 140
+L + ++K ++D+D +DD G+ + +DEP+ P P+ GE+
Sbjct: 165 LLGEILRMIKRVSGIVDDDNFIDDTGVE--------PAYYGSDEPRSPGDAPQAEEGEED 10
Query: 141 DE 142
+E
Sbjct: 9 EE 4
>AI900422
Length = 373
Score = 28.5 bits (62), Expect = 2.5
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 6/56 (10%)
Frame = +3
Query: 29 SVAGTSSYVTSFMNGFSAT------NNNDTKKKHNDNQNPKSPKIKHYQLKALKLL 78
++A SYVTS + T NNN+T +++N NP S Y + A + L
Sbjct: 42 TIASVLSYVTSTRPSLTTTTTTTTNNNNNTTTTNSNNNNPSS----DYSVSAFRAL 197
>TC225443 homologue to UP|RL24_CICAR (O65743) 60S ribosomal protein L24,
complete
Length = 767
Score = 28.5 bits (62), Expect = 2.5
Identities = 18/61 (29%), Positives = 31/61 (50%)
Frame = +2
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKL 197
+DI +++ + RRR + + A KK + E +DAA +A + +ERI+K
Sbjct: 275 KDIAQEAVRKRRRAAKKPYSRSIVGATLEVIQKKRAEKPEVRDAAREAALREIKERIKKT 454
Query: 198 K 198
K
Sbjct: 455 K 457
>TC209388 similar to UP|Q94T09 (Q94T09) NADH dehydrogenase subunit 6, partial
(8%)
Length = 442
Score = 28.5 bits (62), Expect = 2.5
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = -3
Query: 33 TSSYVTSFMNGFSATNNNDTKKKHN 57
TSS+ + M G++ +NND KKK+N
Sbjct: 425 TSSFHFTKMGGYAIIHNNDKKKKNN 351
>TC223418 similar to UP|Q941B8 (Q941B8) At2g13860/F13J11.19, partial (11%)
Length = 490
Score = 28.1 bits (61), Expect = 3.3
Identities = 16/53 (30%), Positives = 25/53 (46%), Gaps = 2/53 (3%)
Frame = +2
Query: 30 VAGTSSYVTSFMNGFSATNNNDTKKKH--NDNQNPKSPKIKHYQLKALKLLDD 80
V G V ++ NNN+ KKK+ N+ K+ H Q+ A KL+ +
Sbjct: 92 VMGDGCAVEGTTGNSNSNNNNNNKKKNKKKKNRGGSKKKMTHEQVLAFKLVSE 250
>TC215643 similar to UP|Q84MC1 (Q84MC1) At4g31290, partial (80%)
Length = 1100
Score = 27.7 bits (60), Expect = 4.2
Identities = 16/56 (28%), Positives = 27/56 (47%), Gaps = 4/56 (7%)
Frame = +3
Query: 2 AEEELNNGYCSSSGDEDGDAAWKAAIDS----VAGTSSYVTSFMNGFSATNNNDTK 53
+EE NG CS+ G+E G ++ + S T+ ++ NG+ A +D K
Sbjct: 675 SEESTWNGKCSTKGEETGWSSTTSTPSSCNNPYPSTAPTARTYCNGYIAEGGDDQK 842
>TC224563 homologue to UP|RL15_ARATH (O23515) 60S ribosomal protein L15,
complete
Length = 1765
Score = 27.3 bits (59), Expect = 5.5
Identities = 7/20 (35%), Positives = 16/20 (80%)
Frame = -2
Query: 49 NNDTKKKHNDNQNPKSPKIK 68
+N T+++HN+ NP+ P+++
Sbjct: 1590 SNSTRERHNEQSNPRLPRLR 1531
>BE803455 similar to GP|4379369|emb| Calmodulin {Physcomitrella patens},
partial (37%)
Length = 172
Score = 26.9 bits (58), Expect = 7.2
Identities = 14/30 (46%), Positives = 20/30 (66%)
Frame = +2
Query: 72 LKALKLLDDILENTIEIVKEPIPVLDEDPN 101
L A+K+ D E+ + KE +PVLD+DPN
Sbjct: 74 LMAIKMNDTDSEDDL---KEALPVLDKDPN 154
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.311 0.131 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,664,286
Number of Sequences: 63676
Number of extensions: 108007
Number of successful extensions: 612
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 592
length of query: 221
length of database: 12,639,632
effective HSP length: 94
effective length of query: 127
effective length of database: 6,654,088
effective search space: 845069176
effective search space used: 845069176
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC147000.5