BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146972.1 + phase: 0 /pseudo
(594 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC220322 187 1e-47
TC232149 73 5e-28
TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F1... 74 2e-13
TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transp... 68 1e-11
BM308209 40 0.004
AI856054 36 0.042
TC206076 similar to UP|O80698 (O80698) F8K4.12, partial (43%) 34 0.16
TC214442 similar to UP|Q84UB0 (Q84UB0) Transcription factor Myb1... 32 0.61
TC212986 31 1.4
TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp... 31 1.8
CF922867 30 3.0
TC215586 homologue to UP|Q8VXT1 (Q8VXT1) Protein kinase 2, parti... 30 3.0
BG725812 30 3.0
TC219288 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {A... 30 3.0
BE474274 weakly similar to PIR|S33622|S33 ADR6 protein - soybean... 30 3.9
CF922397 29 5.2
TC228766 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {A... 29 5.2
TC231412 similar to UP|Q8L7S2 (Q8L7S2) AT3g60070/T2O9_50, partia... 29 5.2
TC226853 weakly similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear prot... 29 5.2
TC215912 similar to GB|AAS09986.1|41618940|AY519516 MYB transcri... 29 6.7
>TC220322
Length = 1264
Score = 187 bits (475), Expect = 1e-47
Identities = 92/173 (53%), Positives = 116/173 (66%), Gaps = 2/173 (1%)
Frame = +2
Query: 319 ECARETTPTQFNRIMERVKSINQKAWEYLNKWPKEAWIKAYFNENCKAYNIVNNACEVFN 378
+CA+ TT +F M +K+IN +AWEYLNKWPK+AW KA+F+ K NI NN CEVFN
Sbjct: 2 QCAKSTTVAEFEGHMAHLKTINCQAWEYLNKWPKQAWTKAHFSTIPKVDNICNNTCEVFN 181
Query: 379 SKILNYRGHPILTMAEEIRCYVMRKMSQNKMKLDGRAGPLCPWQQSRLEKEKIASHNWTP 438
+IL YR PI+TM EEIR Y+MR M+ K+KL G+ GPLC Q RLEKE ++ WTP
Sbjct: 182 FRILQYRCKPIITMLEEIRSYIMRTMAARKVKLSGKPGPLCLVQYKRLEKEFHFANQWTP 361
Query: 439 LWNGDNARQRY--HIENNCRIKVDVDIFKQTCTCRFWQLT*MPCMHACSALAL 489
+W GDN RY H+ N KV+V++ TCTC WQLT MPC HA + + L
Sbjct: 362 IWCGDNMGLRYEVHMWGN---KVEVNLAGWTCTCGVWQLTGMPCRHAIATITL 511
Score = 111 bits (278), Expect = 8e-25
Identities = 51/117 (43%), Positives = 70/117 (59%)
Frame = +1
Query: 478 MPCMHACSALALRGFKLEDHCHAWLTLGSYRETYSYFIQPMNSHIYWEPTPYEKPVPPKV 537
MP H C+ + +G K ED CH WL++ +Y +TY +FI+P+ YW T Y PVPP
Sbjct: 481 MPTCH-CNNNSHKGGKPEDMCHEWLSIEAYNKTYQHFIEPVQGPQYWAQTQYTHPVPPYK 657
Query: 538 KRSAGRPKKNRRKDGNEEPIGGSSMKKTYNDIQCGRCGLMGHNSRSCAKQGVARRPK 594
+ GRPKKNR++ +E+ + G +K+ + CGRCG HN RSC GV RPK
Sbjct: 658 RVQRGRPKKNRKRSVDEDNVTGHKLKRKLAEFTCGRCGQTNHNIRSCKNIGVPVRPK 828
>TC232149
Length = 1005
Score = 72.8 bits (177), Expect(2) = 5e-28
Identities = 34/67 (50%), Positives = 46/67 (67%)
Frame = -1
Query: 319 ECARETTPTQFNRIMERVKSINQKAWEYLNKWPKEAWIKAYFNENCKAYNIVNNACEVFN 378
+CAR T +FN IM+ VK +N+KAW YL+K P+E+W KAYF+ENCK Y E +N
Sbjct: 615 KCARSPTELEFNVIMKVVKRMNEKAWAYLDKGPRESWTKAYFSENCKDY-------ESYN 457
Query: 379 SKILNYR 385
+KIL +
Sbjct: 456 AKILKLK 436
Score = 70.5 bits (171), Expect(2) = 5e-28
Identities = 38/91 (41%), Positives = 55/91 (59%)
Frame = -3
Query: 389 ILTMAEEIRCYVMRKMSQNKMKLDGRAGPLCPWQQSRLEKEKIASHNWTPLWNGDNARQR 448
IL M E+IR Y+M KM +K+K+ R PL P QQSR+EK K+ S+ T W GD R
Sbjct: 427 ILNMCEDIRTYIMCKMKSDKLKMATRLRPLVPMQQSRIEKGKVESNK*TANWVGDPDGTR 248
Query: 449 YHIENNCRIKVDVDIFKQTCTCRFWQLT*MP 479
+ + + +++VD+ + TCR WQL +P
Sbjct: 247 FKV-CHYDTRLNVDLQN*SFTCRMWQLIGIP 158
>TC210254 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;} , partial (6%)
Length = 814
Score = 73.9 bits (180), Expect = 2e-13
Identities = 41/128 (32%), Positives = 61/128 (47%), Gaps = 4/128 (3%)
Frame = +1
Query: 461 VDIFKQTCTCRFWQLT*MPCMHACSALALRGFKLEDHCHAWLTLGSYRETYSYFIQPM-- 518
VDI C+C+ WQLT +PC HA + G D+C + T+ +YR TY+ I P+
Sbjct: 67 VDIDNWDCSCKGWQLTGVPCCHAIAVFECVGRSPYDYCSRYFTVENYRLTYAESIHPVPN 246
Query: 519 --NSHIYWEPTPYEKPVPPKVKRSAGRPKKNRRKDGNEEPIGGSSMKKTYNDIQCGRCGL 576
+ E T +PP KR GRPK + + S+ +QC +C
Sbjct: 247 VDKPPVQGESTALVMVIPPPTKRPPGRPKMKQVE----------SIDIIKRQLQCSKCKG 396
Query: 577 MGHNSRSC 584
+GHN ++C
Sbjct: 397 LGHNRKTC 420
>TC222000 weakly similar to PIR|H84710|H84710 Mutator-like transposase
[imported] - Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (12%)
Length = 617
Score = 68.2 bits (165), Expect = 1e-11
Identities = 39/131 (29%), Positives = 58/131 (43%), Gaps = 7/131 (5%)
Frame = +2
Query: 461 VDIFKQTCTCRFWQLT*MPCMHACSALALRGFKLEDHCHAWLTLGSYRETYSYFIQPMNS 520
V+I +C+CR WQL +PC HA +AL + T S+R+TY+ I P+
Sbjct: 20 VNIGSHSCSCRDWQLNGIPCSHAAAALISCRKDVYAFSQKCFTAASFRDTYAETIHPIPG 199
Query: 521 HIYWEPTPYEK-------PVPPKVKRSAGRPKKNRRKDGNEEPIGGSSMKKTYNDIQCGR 573
+ W T PPK++R GRP+K D + + + + C R
Sbjct: 200 KLEWSKTGNSSMDDNILVVRPPKLRRPPGRPEKRMCVD---------DLNREKHTVHCSR 352
Query: 574 CGLMGHNSRSC 584
C GH R+C
Sbjct: 353 CNQTGHYKRTC 385
>BM308209
Length = 435
Score = 39.7 bits (91), Expect = 0.004
Identities = 18/49 (36%), Positives = 29/49 (58%)
Frame = +2
Query: 334 ERVKSINQKAWEYLNKWPKEAWIKAYFNENCKAYNIVNNACEVFNSKIL 382
E +K +N +A+++L K + W K F+ K ++NN C+ FNS IL
Sbjct: 116 EHIKEVNNEAYDWLIKVGTKLWRKHTFSYYTKCDVLMNNLCDAFNSTIL 262
>AI856054
Length = 173
Score = 36.2 bits (82), Expect = 0.042
Identities = 18/43 (41%), Positives = 24/43 (54%)
Frame = -1
Query: 374 CEVFNSKILNYRGHPILTMAEEIRCYVMRKMSQNKMKLDGRAG 416
CE FNS IL R I++M E+I Y+M + N K+ R G
Sbjct: 140 CESFNSVILEAREKLIVSMLEDIHIYLMERWDTNSKKVVAREG 12
>TC206076 similar to UP|O80698 (O80698) F8K4.12, partial (43%)
Length = 1225
Score = 34.3 bits (77), Expect = 0.16
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +1
Query: 516 QPMNSHIYWEPTPYEKPVPPKVKRSAGR 543
+P ++++WEPTP K PP RS+G+
Sbjct: 157 RPAGANVFWEPTPIAKAPPP*THRSSGK 240
>TC214442 similar to UP|Q84UB0 (Q84UB0) Transcription factor Myb1, partial
(30%)
Length = 1102
Score = 32.3 bits (72), Expect = 0.61
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +2
Query: 571 CGRCGLMGHNSRSCAKQGVARRPK 594
C +CG GHNSR+C G A P+
Sbjct: 212 CSQCGNNGHNSRTCTDGGAAGSPR 283
>TC212986
Length = 404
Score = 31.2 bits (69), Expect = 1.4
Identities = 15/60 (25%), Positives = 31/60 (51%)
Frame = +1
Query: 336 VKSINQKAWEYLNKWPKEAWIKAYFNENCKAYNIVNNACEVFNSKILNYRGHPILTMAEE 395
+++ A E+L++ PK+ W++ + + K + + N E NS N R + ++ EE
Sbjct: 22 IRANKPSAAEWLDQLPKQKWVQCF--DEGKRWGLTTNLSESVNSMFKNTRHLSVSSLVEE 195
>TC226201 similar to UP|O81126 (O81126) 9G8-like SR protein (RSZp22 splicing
factor), partial (72%)
Length = 820
Score = 30.8 bits (68), Expect = 1.8
Identities = 17/54 (31%), Positives = 25/54 (45%)
Frame = +1
Query: 539 RSAGRPKKNRRKDGNEEPIGGSSMKKTYNDIQCGRCGLMGHNSRSCAKQGVARR 592
R GR K + G GG + +D++C CG GH +R C +G + R
Sbjct: 160 RMGGRVKLSHNSRGGG---GGRGGRSGGSDLKCYECGEPGHFARECRMRGGSGR 312
>CF922867
Length = 450
Score = 30.0 bits (66), Expect = 3.0
Identities = 14/37 (37%), Positives = 18/37 (47%)
Frame = +1
Query: 528 PYEKPVPPKVKRSAGRPKKNRRKDGNEEPIGGSSMKK 564
P + P PPK+ + G NRR + P GG KK
Sbjct: 94 PQKTPPPPKLPKKGGPQTPNRRPWEKKTPPGGKKEKK 204
>TC215586 homologue to UP|Q8VXT1 (Q8VXT1) Protein kinase 2, partial (58%)
Length = 858
Score = 30.0 bits (66), Expect = 3.0
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = +2
Query: 181 IIICLSLLMSLLIWRTRTTRSGFWNYSMQILEIMCVM 217
I I SL+++L+ W T S N SMQI+ I+C++
Sbjct: 341 ISIIWSLILNLMHWLGGTVASLGLNLSMQIINILCLL 451
>BG725812
Length = 416
Score = 30.0 bits (66), Expect = 3.0
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 234 WMELYYLLIYVFWIDLIVSM 253
WME+++LL +VFW L+ M
Sbjct: 124 WMEVFFLLTFVFWFMLLFDM 65
>TC219288 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {Arabidopsis
thaliana;} , partial (41%)
Length = 720
Score = 30.0 bits (66), Expect = 3.0
Identities = 10/16 (62%), Positives = 13/16 (80%)
Frame = +2
Query: 570 QCGRCGLMGHNSRSCA 585
+C CG +GHNSR+CA
Sbjct: 32 KCSHCGNIGHNSRTCA 79
>BE474274 weakly similar to PIR|S33622|S33 ADR6 protein - soybean, partial
(37%)
Length = 310
Score = 29.6 bits (65), Expect = 3.9
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -1
Query: 479 PCMHACSALALRGFKLEDHCHAWLTLGSYRETY 511
PC H L R + +HCHAW+ + S + Y
Sbjct: 154 PCTHTPWLLTSRRADMCNHCHAWMKVFSIEKGY 56
>CF922397
Length = 473
Score = 29.3 bits (64), Expect = 5.2
Identities = 15/40 (37%), Positives = 20/40 (49%), Gaps = 3/40 (7%)
Frame = +3
Query: 517 PMNSHIYWEPTPYEKPVP---PKVKRSAGRPKKNRRKDGN 553
P +W PTP +KP P +S G+ KKN + GN
Sbjct: 285 PKPFFFHWGPTPKKKPNPFSFLNPTQSRGKKKKNSKPPGN 404
>TC228766 similar to GB|AAS99672.1|46518381|BT012528 At5g56840 {Arabidopsis
thaliana;} , partial (54%)
Length = 1220
Score = 29.3 bits (64), Expect = 5.2
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +3
Query: 570 QCGRCGLMGHNSRSC 584
+C CG +GHNSR+C
Sbjct: 135 KCSHCGTIGHNSRTC 179
>TC231412 similar to UP|Q8L7S2 (Q8L7S2) AT3g60070/T2O9_50, partial (19%)
Length = 547
Score = 29.3 bits (64), Expect = 5.2
Identities = 21/77 (27%), Positives = 34/77 (43%), Gaps = 10/77 (12%)
Frame = +1
Query: 439 LWNGDNARQRYHIENNCRIKVDVDI--------FKQTCTC--RFWQLT*MPCMHACSALA 488
LW+G +A+ +NC + V + I F + C+C FW * C H C
Sbjct: 322 LWHGTHAQ*-----HNCCLLVHIFIIVLDRYWTFTKECSCCDAFWSSG*WICHHICW*TD 486
Query: 489 LRGFKLEDHCHAWLTLG 505
+ + L+D W+ +G
Sbjct: 487 RQVWTLQDMAWCWIFIG 537
>TC226853 weakly similar to UP|Q9JM93 (Q9JM93) SRp25 nuclear protein
(ADP-ribosylation factor-like 6 interacting protein 4),
partial (11%)
Length = 865
Score = 29.3 bits (64), Expect = 5.2
Identities = 26/97 (26%), Positives = 37/97 (37%), Gaps = 6/97 (6%)
Frame = -3
Query: 500 AWLTLGSYRETYSYFIQPMNSHIYWEPTPYEKPVPPKVKRSAGRPKKNRRKDG------N 553
+WL L S R YS ++ P + S +P NR G +
Sbjct: 692 SWLALASPRRRYSPWLLP--------------------EGSGTKPSGNREPTG*ERALSS 573
Query: 554 EEPIGGSSMKKTYNDIQCGRCGLMGHNSRSCAKQGVA 590
E+P+ GSS + + RC M S SCA G +
Sbjct: 572 EQPVTGSSSLRFQSSFSSNRCWTM-PPSPSCAACGAS 465
>TC215912 similar to GB|AAS09986.1|41618940|AY519516 MYB transcription factor
{Arabidopsis thaliana;} , partial (34%)
Length = 633
Score = 28.9 bits (63), Expect = 6.7
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +2
Query: 570 QCGRCGLMGHNSRSCAKQGV 589
+C C GHNSR+C +GV
Sbjct: 227 RCSHCSHNGHNSRTCPNRGV 286
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.350 0.154 0.574
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,197,317
Number of Sequences: 63676
Number of extensions: 545129
Number of successful extensions: 5876
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 4248
Number of HSP's successfully gapped in prelim test: 160
Number of HSP's that attempted gapping in prelim test: 1450
Number of HSP's gapped (non-prelim): 4656
length of query: 594
length of database: 12,639,632
effective HSP length: 103
effective length of query: 491
effective length of database: 6,081,004
effective search space: 2985772964
effective search space used: 2985772964
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146972.1