BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146941.10 - phase: 0
(124 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC217248 homologue to UP|PM14_ARATH (Q9FMP4) Pre-mRNA branch sit... 246 3e-66
TC234364 similar to UP|ROC5_NICSY (P19684) 33 kDa ribonucleoprot... 50 3e-07
BE475256 similar to SP|P19684|ROC5_ 33 kDa ribonucleoprotein ch... 47 2e-06
TC225340 similar to UP|Q9SPI1 (Q9SPI1) Splicing factor SR1, part... 47 2e-06
TC225640 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, part... 47 3e-06
TC216453 44 2e-05
TC216455 weakly similar to UP|Q83CD7 (Q83CD7) Nucleic acid bindi... 43 3e-05
TC205672 similar to UP|Q9SCL3 (Q9SCL3) PRE-MRNA SPLICING FACTOR ... 43 4e-05
TC204093 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprot... 43 4e-05
TC227813 similar to UP|Q7XXQ1 (Q7XXQ1) Pre-mRNA splicing factor ... 43 4e-05
TC228972 similar to UP|Q8LEI7 (Q8LEI7) Ras-GTPase-activating pro... 43 4e-05
TC225135 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, ... 42 6e-05
TC212985 similar to UP|P82277 (P82277) Plastid-specific 30S ribo... 42 9e-05
TC205700 weakly similar to PIR|T48456|T48456 rna binding protein... 41 2e-04
TC225739 homologue to UP|Q41124 (Q41124) Chloroplast RNA binding... 41 2e-04
TC225576 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, ... 40 2e-04
TC217271 weakly similar to UP|RU2B_HUMAN (P08579) U2 small nucle... 40 2e-04
TC217269 similar to UP|Q41499 (Q41499) Spliceosomal protein, par... 40 2e-04
TC217407 similar to UP|P82277 (P82277) Plastid-specific 30S ribo... 40 3e-04
TC205702 similar to PIR|T48456|T48456 rna binding protein-like -... 40 3e-04
>TC217248 homologue to UP|PM14_ARATH (Q9FMP4) Pre-mRNA branch site p14-like
protein, partial (98%)
Length = 699
Score = 246 bits (627), Expect = 3e-66
Identities = 122/124 (98%), Positives = 122/124 (98%)
Frame = +1
Query: 1 MTTISLRKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTA 60
M ISLRKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTA
Sbjct: 73 MAAISLRKGNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTA 252
Query: 61 FVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGVS 120
FVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGVS
Sbjct: 253 FVVYEDIYDAKTAVDHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYGVS 432
Query: 121 TKDK 124
TKDK
Sbjct: 433 TKDK 444
>TC234364 similar to UP|ROC5_NICSY (P19684) 33 kDa ribonucleoprotein,
chloroplast precursor, partial (32%)
Length = 710
Score = 50.1 bits (118), Expect = 3e-07
Identities = 28/79 (35%), Positives = 44/79 (55%), Gaps = 3/79 (3%)
Frame = +2
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDT---RGTAFVVYEDIYDAKTAVDHL 77
LYV NLP++IT+ E+ ++FG+ G + + I ++ T RG AFV + DAK A+
Sbjct: 383 LYVGNLPYSITNSELGELFGEAGTVASVEIVYDRVTDRSRGFAFVTMGSVEDAKEAIRMF 562
Query: 78 SGFNVANRYLIVLYYQQAK 96
G V R + V + + K
Sbjct: 563 DGSQVGGRTVXVNFPEVPK 619
>BE475256 similar to SP|P19684|ROC5_ 33 kDa ribonucleoprotein chloroplast
precursor. [Wood tobacco] {Nicotiana sylvestris},
partial (26%)
Length = 411
Score = 47.4 bits (111), Expect = 2e-06
Identities = 25/68 (36%), Positives = 39/68 (56%), Gaps = 3/68 (4%)
Frame = +2
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDT---RGTAFVVYEDIYDAKTAVDHL 77
LYV NLP++IT+ + ++FG+ G + + I ++ T RG AFV ++ DAK A+
Sbjct: 194 LYVGNLPYSITNSALAELFGEAGTVASVEIMYDRVTDRSRGFAFVTMGNVEDAKEAIRMF 373
Query: 78 SGFNVANR 85
G V R
Sbjct: 374 DGSQVGGR 397
>TC225340 similar to UP|Q9SPI1 (Q9SPI1) Splicing factor SR1, partial (81%)
Length = 1349
Score = 47.0 bits (110), Expect = 2e-06
Identities = 25/64 (39%), Positives = 36/64 (56%)
Frame = +1
Query: 18 NRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDIYDAKTAVDHL 77
+R +YV NLP +I E+ D+F KYG I I + G AFV +ED DA+ A+
Sbjct: 157 SRTVYVGNLPGDIREREVEDLFLKYGHITHIDLKVPPRPPGYAFVEFEDAQDAEDAIRGR 336
Query: 78 SGFN 81
G++
Sbjct: 337 DGYD 348
>TC225640 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, partial (66%)
Length = 1290
Score = 46.6 bits (109), Expect = 3e-06
Identities = 25/72 (34%), Positives = 39/72 (53%), Gaps = 3/72 (4%)
Frame = +3
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDT---RGTAFVVYEDIYDAKTAVDHL 77
++V NLPF+I SE + +FG+ G + + N+ T RG FV + + K AV+
Sbjct: 447 IFVGNLPFDIDSENLASLFGQAGTVEVAEVIYNRATDRSRGFGFVTMSTLEELKKAVEMF 626
Query: 78 SGFNVANRYLIV 89
SG+ + R L V
Sbjct: 627 SGYELNGRVLTV 662
>TC216453
Length = 1075
Score = 43.9 bits (102), Expect = 2e-05
Identities = 26/89 (29%), Positives = 49/89 (54%), Gaps = 2/89 (2%)
Frame = +1
Query: 14 PPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKD--TRGTAFVVYEDIYDAK 71
P + + LYV NL +++T+ ++ D+F + G + + I +KD ++G AFV +A+
Sbjct: 202 PTDNVKKLYVVNLSWSLTAADITDLFAQCGTVTDVEIIKSKDGRSKGYAFVTMASGEEAQ 381
Query: 72 TAVDHLSGFNVANRYLIVLYYQQAKMSKK 100
AVD + ++ R ++ + AK KK
Sbjct: 382 AAVDKFDSYELSGR---IIRVELAKRLKK 459
>TC216455 weakly similar to UP|Q83CD7 (Q83CD7) Nucleic acid binding domain
protein, partial (35%)
Length = 491
Score = 43.1 bits (100), Expect = 3e-05
Identities = 23/78 (29%), Positives = 44/78 (55%), Gaps = 2/78 (2%)
Frame = +2
Query: 14 PPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKD--TRGTAFVVYEDIYDAK 71
P + + LYV NL +++T+ ++ D+F + G + + I +KD ++G AFV +A+
Sbjct: 221 PTDNVKKLYVVNLSWSLTAADINDLFAQSGTVTDVEIIKSKDGRSKGYAFVTMASGEEAQ 400
Query: 72 TAVDHLSGFNVANRYLIV 89
AVD + ++ R + V
Sbjct: 401 AAVDKFDSYELSGRIIRV 454
>TC205672 similar to UP|Q9SCL3 (Q9SCL3) PRE-MRNA SPLICING FACTOR SF2-like
protein, partial (73%)
Length = 1155
Score = 42.7 bits (99), Expect = 4e-05
Identities = 22/64 (34%), Positives = 34/64 (52%)
Frame = +2
Query: 18 NRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAFVVYEDIYDAKTAVDHL 77
+R +YV NLP +I E+ D+F KYG I I + FV +++ DA+ A+
Sbjct: 143 SRTIYVGNLPSDIRESEIEDLFYKYGRIMDIELKVPPRPPCYCFVEFDNARDAEDAIRGR 322
Query: 78 SGFN 81
G+N
Sbjct: 323 DGYN 334
>TC204093 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprotein A,
chloroplast precursor (CP29A), partial (63%)
Length = 1403
Score = 42.7 bits (99), Expect = 4e-05
Identities = 22/72 (30%), Positives = 38/72 (52%), Gaps = 3/72 (4%)
Frame = +2
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDT---RGTAFVVYEDIYDAKTAVDHL 77
L+V NLPFN+ S ++ ++F G + + + +K T RG FV + +A+ A
Sbjct: 335 LFVGNLPFNVDSAQLAELFESAGNVEVVEVIYDKTTGRSRGFGFVTMSSVEEAEAAAQQF 514
Query: 78 SGFNVANRYLIV 89
+G+ + R L V
Sbjct: 515 NGYELDGRALRV 550
>TC227813 similar to UP|Q7XXQ1 (Q7XXQ1) Pre-mRNA splicing factor (Fragment),
partial (77%)
Length = 1322
Score = 42.7 bits (99), Expect = 4e-05
Identities = 30/104 (28%), Positives = 51/104 (48%), Gaps = 3/104 (2%)
Frame = +2
Query: 18 NRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNK---DTRGTAFVVYEDIYDAKTAV 74
N L VRN+P + EE+ F ++G +R + I + + RG AFV + D YDA A
Sbjct: 293 NGSLLVRNIPLDCRPEELRVPFERFGPVRDVYIPKDYYSGEPRGFAFVQFVDPYDASEAQ 472
Query: 75 DHLSGFNVANRYLIVLYYQQAKMSKKFDQKKKEDEIARMQEKYG 118
H++ A R + V+ ++ + K+ ++ + R YG
Sbjct: 473 YHMNRQIFAGREISVVVAEETR--KRPEEMRHRTSRFRGPASYG 598
>TC228972 similar to UP|Q8LEI7 (Q8LEI7) Ras-GTPase-activating protein
SH3-domain binding protein-like, partial (19%)
Length = 918
Score = 42.7 bits (99), Expect = 4e-05
Identities = 23/71 (32%), Positives = 42/71 (58%), Gaps = 2/71 (2%)
Frame = +3
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQ--IRIGTNKDTRGTAFVVYEDIYDAKTAVDHLS 78
+Y+RNLPFN T E++ ++F K+G I+ I++ ++K FV +E++ +A++ S
Sbjct: 9 IYIRNLPFNATVEQLEEVFKKFGPIKHGGIQVRSSKHGFCFGFVEFEELSSMHSALE-AS 185
Query: 79 GFNVANRYLIV 89
V R +V
Sbjct: 186PITVGERQAVV 218
>TC225135 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, partial
(97%)
Length = 2392
Score = 42.4 bits (98), Expect = 6e-05
Identities = 25/90 (27%), Positives = 48/90 (52%), Gaps = 3/90 (3%)
Frame = +3
Query: 17 VNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIG---TNKDTRGTAFVVYEDIYDAKTA 73
V LYV +L N+T ++YD+F + G + +R+ T++ + G +V + + DA A
Sbjct: 174 VTTSLYVGDLDPNVTDAQLYDLFNQLGQVVSVRVCRDLTSRRSLGYGYVNFSNPQDAARA 353
Query: 74 VDHLSGFNVANRYLIVLYYQQAKMSKKFDQ 103
+D L+ + NR + ++Y + +K Q
Sbjct: 354 LDVLNFTPLNNRPIRIMYSHRDPSIRKSGQ 443
>TC212985 similar to UP|P82277 (P82277) Plastid-specific 30S ribosomal
protein 2, chloroplast precursor (PSRP-2), partial (27%)
Length = 423
Score = 41.6 bits (96), Expect = 9e-05
Identities = 22/70 (31%), Positives = 39/70 (55%), Gaps = 3/70 (4%)
Frame = +2
Query: 19 RVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNK---DTRGTAFVVYEDIYDAKTAVD 75
R LYV N+P +T+EE+ I ++GA+ + + +K +R AFV + + DA ++
Sbjct: 203 RRLYVGNIPRTVTNEELAKIVQEHGAVEKAEVMYDKYSGRSRRFAFVTMKTVEDATAVIE 382
Query: 76 HLSGFNVANR 85
L+G + R
Sbjct: 383 KLNGTEIGGR 412
>TC205700 weakly similar to PIR|T48456|T48456 rna binding protein-like -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(83%)
Length = 1176
Score = 40.8 bits (94), Expect = 2e-04
Identities = 24/71 (33%), Positives = 36/71 (49%), Gaps = 4/71 (5%)
Frame = +3
Query: 14 PPE-VNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNK---DTRGTAFVVYEDIYD 69
PPE VLYV +P +EM FG++G I+++RI NK +R F+ +E
Sbjct: 306 PPENTATVLYVGRIPHGFYEKEMEGYFGQFGTIKRLRIARNKKSGKSRHFGFIEFESPEV 485
Query: 70 AKTAVDHLSGF 80
AK D + +
Sbjct: 486 AKIVADTMHNY 518
>TC225739 homologue to UP|Q41124 (Q41124) Chloroplast RNA binding protein
precursor, partial (85%)
Length = 1436
Score = 40.8 bits (94), Expect = 2e-04
Identities = 24/79 (30%), Positives = 39/79 (48%), Gaps = 3/79 (3%)
Frame = +3
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDT---RGTAFVVYEDIYDAKTAVDHL 77
LY NLP+++ S ++ + YG+ I + ++DT RG AFV I D +++L
Sbjct: 504 LYFGNLPYSVDSAKLAGLIQDYGSAELIEVLYDRDTGKSRGFAFVTMSCIEDCNAVIENL 683
Query: 78 SGFNVANRYLIVLYYQQAK 96
G R L V + + K
Sbjct: 684 DGKEFLGRTLRVNFSSKPK 740
>TC225576 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, partial
(93%)
Length = 2491
Score = 40.4 bits (93), Expect = 2e-04
Identities = 25/67 (37%), Positives = 38/67 (56%), Gaps = 2/67 (2%)
Frame = +3
Query: 21 LYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKD--TRGTAFVVYEDIYDAKTAVDHLS 78
+YV+NL + T EE+ FG+YG I I + D +R FV +E+ DA AV+ L+
Sbjct: 903 VYVKNLSESTTDEELMKFFGEYGTITSAVIMRDADGKSRCFGFVNFENPDDAAKAVEGLN 1082
Query: 79 GFNVANR 85
G V ++
Sbjct: 1083GKKVDDK 1103
Score = 25.8 bits (55), Expect = 5.4
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +1
Query: 17 VNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRI 50
V LYV +L N+ ++YD+F + + +R+
Sbjct: 355 VTTSLYVGDLEQNVNDAQLYDLFNQVVQVVSVRV 456
>TC217271 weakly similar to UP|RU2B_HUMAN (P08579) U2 small nuclear
ribonucleoprotein B", partial (48%)
Length = 744
Score = 40.4 bits (93), Expect = 2e-04
Identities = 26/94 (27%), Positives = 46/94 (48%), Gaps = 4/94 (4%)
Frame = +3
Query: 3 TISLRKG----NTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRG 58
T S R+G T P N +L++ NLP T + +F +Y +++R+ K G
Sbjct: 270 TASFRQGPGAQETAAP---NNILFIENLPHETTGRMLEMLFEQYPGFKEVRLIEAKP--G 434
Query: 59 TAFVVYEDIYDAKTAVDHLSGFNVANRYLIVLYY 92
AFV +ED + A+ L GF + + +++ +
Sbjct: 435 IAFVDFEDEVQSSMAMQALHGFKITPQNPMIITF 536
>TC217269 similar to UP|Q41499 (Q41499) Spliceosomal protein, partial (51%)
Length = 762
Score = 40.4 bits (93), Expect = 2e-04
Identities = 24/91 (26%), Positives = 45/91 (49%), Gaps = 1/91 (1%)
Frame = +2
Query: 3 TISLRKG-NTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNKDTRGTAF 61
T S R+G + N +L++ NLP T + +F +Y +++R+ K G AF
Sbjct: 224 TASFRQGPGAQEAAAPNNILFIENLPHETTGRMLEMLFEQYPGFKEVRLIEAKP--GIAF 397
Query: 62 VVYEDIYDAKTAVDHLSGFNVANRYLIVLYY 92
V +ED + A+ L GF + + +++ +
Sbjct: 398 VDFEDEVQSSMAMQALHGFKITPQNPMIITF 490
>TC217407 similar to UP|P82277 (P82277) Plastid-specific 30S ribosomal
protein 2, chloroplast precursor (PSRP-2), partial (68%)
Length = 1194
Score = 40.0 bits (92), Expect = 3e-04
Identities = 22/74 (29%), Positives = 41/74 (54%), Gaps = 3/74 (4%)
Frame = +2
Query: 19 RVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNK---DTRGTAFVVYEDIYDAKTAVD 75
R LYV N+P +T++E+ I ++GA+ + + +K +R AFV + + DA ++
Sbjct: 296 RRLYVGNIPRTVTNDELAKIVQEHGAVEKAEVMYDKYSGRSRRFAFVTMKTVEDATAVIE 475
Query: 76 HLSGFNVANRYLIV 89
L+G + R + V
Sbjct: 476 KLNGTELGGREIKV 517
>TC205702 similar to PIR|T48456|T48456 rna binding protein-like - Arabidopsis
thaliana {Arabidopsis thaliana;} , partial (34%)
Length = 472
Score = 40.0 bits (92), Expect = 3e-04
Identities = 24/66 (36%), Positives = 34/66 (51%), Gaps = 4/66 (6%)
Frame = +3
Query: 14 PPE-VNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGTNK---DTRGTAFVVYEDIYD 69
PPE VLYV +P +EM FG++G I+++RI NK +R F+ +E
Sbjct: 273 PPENTATVLYVGRIPHGFYEKEMEGYFGQFGTIKRLRIARNKKSGKSRHFGFIEFESPEV 452
Query: 70 AKTAVD 75
AK D
Sbjct: 453 AKIVAD 470
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.136 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,599,694
Number of Sequences: 63676
Number of extensions: 31920
Number of successful extensions: 288
Number of sequences better than 10.0: 108
Number of HSP's better than 10.0 without gapping: 282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 283
length of query: 124
length of database: 12,639,632
effective HSP length: 85
effective length of query: 39
effective length of database: 7,227,172
effective search space: 281859708
effective search space used: 281859708
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146941.10