BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146865.13 - phase: 0
(592 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BM085746 184 1e-46
TC206111 similar to UP|O22208 (O22208) BZIP family transcription... 33 0.46
BM527349 32 0.61
TC208060 weakly similar to UP|Q840B9 (Q840B9) Endo-b1,4-mannanas... 32 1.0
TC217217 similar to GB|AAO44081.1|28466945|BT004815 At3g57000 {A... 31 1.4
TC211496 similar to UP|Q8RW17 (Q8RW17) Aux/IAA protein, partial ... 30 3.0
TC217464 similar to GB|AAD31078.1|4850408|F3F19 Contains PF|0009... 30 3.9
TC233873 weakly similar to UP|Q6ZJC8 (Q6ZJC8) BHLH protein famil... 30 3.9
TC228640 similar to UP|Q8RW17 (Q8RW17) Aux/IAA protein, partial ... 30 3.9
TC235164 30 3.9
BM527625 similar to GP|15450619|gb| AT3g14110/MAG2_6 {Arabidopsi... 30 3.9
TC224907 weakly similar to UP|Q6Z8A6 (Q6Z8A6) Zinc finger (C3HC4... 29 5.1
TC209766 similar to GB|AAP31952.1|30387573|BT006608 At1g12680 {A... 29 6.7
BG726018 29 6.7
BI893397 29 6.7
TC217291 similar to UP|WR30_ARATH (Q9FL62) Probable WRKY transcr... 28 8.8
TC219609 similar to UP|PER1_ARAHY (P22195) Cationic peroxidase 1... 28 8.8
BU965205 28 8.8
>BM085746
Length = 421
Score = 184 bits (466), Expect = 1e-46
Identities = 99/142 (69%), Positives = 110/142 (76%)
Frame = -2
Query: 184 LNKGLDLKIKGVVNKKKRNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQNICRNSEERC 243
LNKGL+L+IK VVNKKKR PNRKRA ND SKP R EEE GV+N+SQSSQN+C NS+ERC
Sbjct: 420 LNKGLNLEIKPVVNKKKRKPNRKRAANDASKPISRPEEETGVQNASQSSQNMCGNSKERC 241
Query: 244 FEQDGDEHLPLVKRWRVRMGKSSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRG 303
FEQDGDEHLPLVKR RVRMGK SS E EL+S K+CKE+ NS QMI SNCEN
Sbjct: 240 FEQDGDEHLPLVKRARVRMGK-SSVEAELHSTLQCLEKNCKENTNSVQQMITPSNCENNS 64
Query: 304 SADVGSSVLIGTMDNVSPSKNF 325
AD SSVL G +D+VSP KNF
Sbjct: 63 PADGDSSVLNGALDDVSP-KNF 1
>TC206111 similar to UP|O22208 (O22208) BZIP family transcription factor,
partial (36%)
Length = 1627
Score = 32.7 bits (73), Expect = 0.46
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +3
Query: 514 SPALSLPPNIEANIITSNHSNTTSNASEHNRINLHSVAD 552
SP +P AN+ T NH N TS NR LH + +
Sbjct: 942 SPGAIVPATSVANVSTENHQNATSVKKTRNRRTLHELPE 1058
>BM527349
Length = 408
Score = 32.3 bits (72), Expect = 0.61
Identities = 22/84 (26%), Positives = 34/84 (40%), Gaps = 4/84 (4%)
Frame = +1
Query: 1 MKSSNSDMDSHGLVCPAEDDSAAVLKDESHDNEASKELTENV----ASVHSAKPLTYSSR 56
M +N+ + G P+ D+A L + N K A V S P T
Sbjct: 160 MLDNNNSASNSG--APSSSDAAFALSENGVANNKRKRRPAGTPDPDAEVVSLSPTTLLES 333
Query: 57 KRSAAELCPQGFITDRHMPVRKNR 80
R E+C QGF D+++ + + R
Sbjct: 334 DRYVCEICNQGFQRDQNLQMHRRR 405
>TC208060 weakly similar to UP|Q840B9 (Q840B9) Endo-b1,4-mannanase 26B,
partial (5%)
Length = 2230
Score = 31.6 bits (70), Expect = 1.0
Identities = 32/135 (23%), Positives = 57/135 (41%), Gaps = 3/135 (2%)
Frame = +1
Query: 422 DSSHIIVHSISANSNPMISTENKLSNQVDEPSTRFQPQETGKNVLQCAADQIEELSDFVV 481
DS+ H IS+N +PM + + + PS P + + IE+ S
Sbjct: 547 DSAASDAHHISSNGSPMAAPRDLELSPAAGPSPVVHPDSPSEET-----EHIEKFS---- 699
Query: 482 SHTANVDLKTQVHGET--YPDLDSKCNEAES-NQDSPALSLPPNIEANIITSNHSNTTSN 538
+ N D+ G+T + DLD S +Q + P N+E ++ +S+ S +
Sbjct: 700 --SNNDDIFQIQQGKTNIHQDLDKNTVAFTSGSQHEIDVKSPSNLENDLSSSDKSVVAQS 873
Query: 539 ASEHNRINLHSVADV 553
+S+H+ I + V
Sbjct: 874 SSDHDEIQSSNAIQV 918
>TC217217 similar to GB|AAO44081.1|28466945|BT004815 At3g57000 {Arabidopsis
thaliana;} , partial (51%)
Length = 1080
Score = 31.2 bits (69), Expect = 1.4
Identities = 22/91 (24%), Positives = 43/91 (47%)
Frame = +3
Query: 439 ISTENKLSNQVDEPSTRFQPQETGKNVLQCAADQIEELSDFVVSHTANVDLKTQVHGETY 498
+ KL + P T++ P + K L +++++ ++ D+V + T+N+DL V +
Sbjct: 552 VGKREKLLRTIKNPVTQYLPINSRKIGLSYSSEKLVDMDDYVSTVTSNMDLVFVVGAMAH 731
Query: 499 PDLDSKCNEAESNQDSPALSLPPNIEANIIT 529
+ E + +D A+S P A IT
Sbjct: 732 GKI-----ETDYTEDYVAISGYPLSAAYCIT 809
>TC211496 similar to UP|Q8RW17 (Q8RW17) Aux/IAA protein, partial (22%)
Length = 442
Score = 30.0 bits (66), Expect = 3.0
Identities = 20/76 (26%), Positives = 31/76 (40%)
Frame = +2
Query: 159 SLNEGSAMDSNFKHTETSECPEEVELNKGLDLKIKGVVNKKKRNPNRKRATNDTSKPTIR 218
S++ S D+ +E S CPEE EL GL L + + K + + R P+
Sbjct: 77 SMSTVSKDDNLALSSEDSSCPEESELELGLGLSLSSGSSSKSHHHHHARIYTAKDFPSSA 256
Query: 219 VEEELGVRNSSQSSQN 234
+ S SS +
Sbjct: 257 AAAAAAAASPSSSSSS 304
>TC217464 similar to GB|AAD31078.1|4850408|F3F19 Contains PF|00097 Zinc
finger (C3HC4) ring finger motif. {Arabidopsis
thaliana;} , partial (59%)
Length = 699
Score = 29.6 bits (65), Expect = 3.9
Identities = 21/69 (30%), Positives = 31/69 (44%)
Frame = +2
Query: 351 LPPSKRLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAINDHEGGGL 410
LP +RLH +LE + EEGQ+ +E S I +K + +V
Sbjct: 140 LPSLQRLHGSLEKLEVVKEEEGQSSIEG-----PSYGKKVIEGVKLTTNV---------- 274
Query: 411 ELQKFDACG 419
+LQ+ D CG
Sbjct: 275 DLQREDECG 301
>TC233873 weakly similar to UP|Q6ZJC8 (Q6ZJC8) BHLH protein family-like,
partial (25%)
Length = 817
Score = 29.6 bits (65), Expect = 3.9
Identities = 41/174 (23%), Positives = 63/174 (35%), Gaps = 2/174 (1%)
Frame = +3
Query: 30 HD-NEASKELTENVASVHSAKPLTYSSRKRSAAELCPQGFITDRHMPVRKNRSSSRVQPF 88
HD A L + + A+ S +R + E C +R R N S ++
Sbjct: 45 HDAGHAQSSLIKKAVELDGAR----SKTERKSTEACKSHREAERRRRQRINSHLSTLRTL 212
Query: 89 MFPCNDSGKNAGSQLTNAAQGASVRRNKRLRKSPDLAGCNDFDSSALVLNGSMEDKDNSS 148
+ NA + G V KRLRK D C D SS GS+ + +
Sbjct: 213 L-------PNAAKSDKASLLGEVVEHVKRLRKQADDVTCGDSYSSRSGEPGSVR---SEA 362
Query: 149 EILTNDSDEFSLNEGSAMDSNFKHTE-TSECPEEVELNKGLDLKIKGVVNKKKR 201
+ DE +++ D K + T C + LN+ + I+ V K R
Sbjct: 363 WPFPGECDEVTVSYCDGEDGEPKRVKATVCCGDRTGLNRDVSQAIRSVRAKAVR 524
>TC228640 similar to UP|Q8RW17 (Q8RW17) Aux/IAA protein, partial (51%)
Length = 1344
Score = 29.6 bits (65), Expect = 3.9
Identities = 26/84 (30%), Positives = 36/84 (41%), Gaps = 7/84 (8%)
Frame = +2
Query: 159 SLNEGSAMDSNFKHTETSECPEEVELNKGLDLKIKGVVNKKKRN-----PNRKRATNDTS 213
S++ S D+ +E S CPEE EL GL L + + K + P A T+
Sbjct: 143 SMSTVSKDDNLVLSSEDSSCPEESELELGLGLSLSSGPSSKSHHHHVHAPTTLYARIYTA 322
Query: 214 K--PTIRVEEELGVRNSSQSSQNI 235
K P+ +SS SS NI
Sbjct: 323 KDFPSSAAAASSSPSSSSSSSPNI 394
>TC235164
Length = 797
Score = 29.6 bits (65), Expect = 3.9
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = -2
Query: 265 SSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRGS 304
SSS E L+ +P + G+SC +PP ++AS E R S
Sbjct: 346 SSSIETNLSRLPRSDGQSC-----APPSLLASVAAEARDS 242
>BM527625 similar to GP|15450619|gb| AT3g14110/MAG2_6 {Arabidopsis thaliana},
partial (13%)
Length = 427
Score = 29.6 bits (65), Expect = 3.9
Identities = 27/90 (30%), Positives = 41/90 (45%)
Frame = -1
Query: 347 CEAALPPSKRLHRALEAMSANAAEEGQAHVESSASRMTSIATCCISSIKTSPDVAINDHE 406
C A+ PP+ + MSA A+ G E SAS +++ A I +IKT I+D
Sbjct: 394 CRASNPPT------VAPMSATASSIGILKTELSASHVSAKAVLGIENIKTLLATNISDGN 233
Query: 407 GGGLELQKFDACGGGDSSHIIVHSISANSN 436
+ F+ C S H I+ S+ S+
Sbjct: 232 LWCMSSLHFEQC----SKHEILEDPSSVSS 155
>TC224907 weakly similar to UP|Q6Z8A6 (Q6Z8A6) Zinc finger (C3HC4-type RING
finger)-like, partial (12%)
Length = 1685
Score = 29.3 bits (64), Expect = 5.1
Identities = 24/82 (29%), Positives = 34/82 (41%)
Frame = +2
Query: 199 KKRNPNRKRATNDTSKPTIRVEEELGVRNSSQSSQNICRNSEERCFEQDGDEHLPLVKRW 258
+ R+P R R+ D S+PT RVE R +S S S R + E LP R
Sbjct: 173 RSRSPVRSRSPVDRSRPT-RVE-----RRASSSRSRSPSKSRSRSRSRSSVESLPRASRD 334
Query: 259 RVRMGKSSSTEGELNSIPHTPG 280
S S +G+ + + G
Sbjct: 335 SRSRSSSRSPDGKKGLVSYGDG 400
>TC209766 similar to GB|AAP31952.1|30387573|BT006608 At1g12680 {Arabidopsis
thaliana;} , partial (29%)
Length = 585
Score = 28.9 bits (63), Expect = 6.7
Identities = 28/107 (26%), Positives = 46/107 (42%), Gaps = 7/107 (6%)
Frame = -3
Query: 429 HSISANSNPMISTENKLSNQVDEPSTRFQPQETGK------NVLQCAADQIEELSDFVVS 482
H + SNP+ + L++ +DEP+TR Q K N LQC ++ V
Sbjct: 490 HFLEYISNPLFRIQTILNHPIDEPTTRAQFHHKMKAFSFLINSLQCNNPRMSG----*VL 323
Query: 483 HTANVDLKTQV-HGETYPDLDSKCNEAESNQDSPALSLPPNIEANII 528
H N+ + + + + S N + + P L+LP + NII
Sbjct: 322 HDLNLSMNRLLPFLQGLTRIFSSTNL*PTQPNRPELALPDCLPRNII 182
>BG726018
Length = 412
Score = 28.9 bits (63), Expect = 6.7
Identities = 25/113 (22%), Positives = 48/113 (42%), Gaps = 8/113 (7%)
Frame = +1
Query: 140 SMEDKDNSSEILTNDSDEFSLNEGSAMDSNFKHTETSECPEEVELNKGLDLKIKGVVNKK 199
++ +++ SS S+E +++ A K +E + + E+ K D K N K
Sbjct: 67 TVAEEERSSSTPKKASNE--IDKIFAAGKKRKKSEMEKTGKSGEVTKKTDKTKKKKKNLK 240
Query: 200 KRNPNRKRATNDTSKP--------TIRVEEELGVRNSSQSSQNICRNSEERCF 244
+ + + + S+P T+ EEELG+ N+ + +C CF
Sbjct: 241 RNQSDNGKFVDLPSRPRKKTEDGLTVYTEEELGISNADAGNTPLCPFDCSCCF 399
>BI893397
Length = 429
Score = 28.9 bits (63), Expect = 6.7
Identities = 15/46 (32%), Positives = 22/46 (47%)
Frame = +2
Query: 520 PPNIEANIITSNHSNTTSNASEHNRINLHSVADVMKKEIISPNLDP 565
PP A S T+ A+ R LHS+ +++ +PNLDP
Sbjct: 251 PPT*SAPASFLGKSRTSETAAPSLRSTLHSILSTWEEKSPAPNLDP 388
>TC217291 similar to UP|WR30_ARATH (Q9FL62) Probable WRKY transcription
factor 30 (WRKY DNA-binding protein 30), partial (21%)
Length = 1108
Score = 28.5 bits (62), Expect = 8.8
Identities = 22/81 (27%), Positives = 36/81 (44%)
Frame = +1
Query: 263 GKSSSTEGELNSIPHTPGKSCKEDINSPPQMIASSNCENRGSADVGSSVLIGTMDNVSPS 322
G SS E ++ I H G +SP ++ S N + D SS+ TM +
Sbjct: 553 GSSSGLEVKIEDIDHKEGIFPSFCFSSP-SIVGSENGD-----DNNSSIFSYTMIENNLM 714
Query: 323 KNFTPCFENQVCNTKKDQTFC 343
++F+P F + + + TFC
Sbjct: 715 ESFSPAFISPTTSDIESNTFC 777
>TC219609 similar to UP|PER1_ARAHY (P22195) Cationic peroxidase 1 precursor
(PNPC1) , partial (79%)
Length = 1036
Score = 28.5 bits (62), Expect = 8.8
Identities = 20/62 (32%), Positives = 28/62 (44%)
Frame = +2
Query: 216 TIRVEEELGVRNSSQSSQNICRNSEERCFEQDGDEHLPLVKRWRVRMGKSSSTEGELNSI 275
TIR E E VRN S+ ++ R CF Q D V + +++ GE NS
Sbjct: 197 TIRKEVERAVRNESRMGASLLRLHFHDCFVQGCDA--------SVLLDDTANFTGEKNSF 352
Query: 276 PH 277
P+
Sbjct: 353 PN 358
>BU965205
Length = 444
Score = 28.5 bits (62), Expect = 8.8
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -1
Query: 502 DSKCNEAESNQDSPALSLPPNIEANIITSNHSN 534
D KC E ESN+D L N + + T+NH +
Sbjct: 399 DDKCQELESNRDDTDLPQHRNTQLSNQTNNHQS 301
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.308 0.124 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,904,432
Number of Sequences: 63676
Number of extensions: 330950
Number of successful extensions: 1360
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1352
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1359
length of query: 592
length of database: 12,639,632
effective HSP length: 103
effective length of query: 489
effective length of database: 6,081,004
effective search space: 2973610956
effective search space used: 2973610956
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146865.13