BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.25 + phase: 0
(206 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC210086 similar to UP|NS12_ARATH (Q9LNH6) Novel plant SNARE 12 ... 189 1e-48
TC231322 similar to GB|AAC61818.2|20197215|AC004667 expressed pr... 152 1e-37
TC209508 similar to UP|NS13_ARATH (Q9LRP1) Novel plant SNARE 13 ... 132 1e-31
BI974298 115 2e-26
AW830126 similar to GP|20197215|gb| expressed protein {Arabidops... 88 3e-18
AI416848 similar to GP|20197215|gb| expressed protein {Arabidops... 70 9e-13
BI971096 weakly similar to PIR|C96522|C965 F11A17.20 [imported] ... 44 5e-05
TC228521 32 0.15
TC224492 similar to UP|Q9VJD4 (Q9VJD4) CG5094-PA (LD24721p), par... 31 0.35
TC229683 similar to GB|AAM62553.1|21553460|AY085322 snap25a {Ara... 31 0.45
TC220878 weakly similar to UP|Q84TG3 (Q84TG3) At2g35930, partial... 30 0.59
TC232996 similar to UP|Q8S3B4 (Q8S3B4) NADH-ubiquinone oxidoredu... 30 1.0
TC214818 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous hea... 29 1.3
TC209651 weakly similar to UP|Q9C887 (Q9C887) Myosin-like protei... 29 1.3
BE823260 29 1.3
TC219027 similar to UP|Q94KD3 (Q94KD3) At1g71270/F3I17_8, partia... 29 1.7
BU547613 similar to GP|15528811|dbj P0431G06.18 {Oryza sativa (j... 28 2.2
TC225329 similar to UP|ATPX_SPIOL (P31853) ATP synthase B' chain... 28 2.9
TC220324 similar to GB|AAP13360.1|30023654|BT006252 At4g30480 {A... 28 3.8
TC205607 homologue to UP|Q6YIV8 (Q6YIV8) Cytochrome b (Fragment)... 27 8.5
>TC210086 similar to UP|NS12_ARATH (Q9LNH6) Novel plant SNARE 12 (AtNPSN12),
partial (49%)
Length = 799
Score = 189 bits (479), Expect = 1e-48
Identities = 97/101 (96%), Positives = 100/101 (98%)
Frame = +1
Query: 106 SNQELVNAGMKTMDETDQAIERSKQVVHQTIEVGTQTASTLKGQTEQMGRIVNELDSIQF 165
SNQEL+NAG KTMDETDQAIERSKQVVHQTIEVGTQTA+TLKGQTEQMGRIVNELDSIQF
Sbjct: 7 SNQELINAGTKTMDETDQAIERSKQVVHQTIEVGTQTAATLKGQTEQMGRIVNELDSIQF 186
Query: 166 SIKKASQLVKEIGRQVATDKCIMLFLFLIVCGVIAIIVVKV 206
SIKKASQLVKEIGRQVATDKCIMLFLFLIVCGVIAIIVVK+
Sbjct: 187 SIKKASQLVKEIGRQVATDKCIMLFLFLIVCGVIAIIVVKI 309
>TC231322 similar to GB|AAC61818.2|20197215|AC004667 expressed protein
{Arabidopsis thaliana;} , partial (58%)
Length = 936
Score = 152 bits (383), Expect = 1e-37
Identities = 79/111 (71%), Positives = 92/111 (82%)
Frame = +2
Query: 96 EGNVQLASEMSNQELVNAGMKTMDETDQAIERSKQVVHQTIEVGTQTASTLKGQTEQMGR 155
E N LAS M+N++L++ G + MDETDQAIER K+VV TI VGT TA+ LK QTEQM R
Sbjct: 104 EENGLLASSMTNEQLMDHGNRMMDETDQAIERGKKVVQDTINVGTDTAAALKAQTEQMSR 283
Query: 156 IVNELDSIQFSIKKASQLVKEIGRQVATDKCIMLFLFLIVCGVIAIIVVKV 206
+VNELDSI FS+KKAS+LVKEIGRQVATDKCIM LFLIV GVIAII+VK+
Sbjct: 284 VVNELDSIHFSMKKASKLVKEIGRQVATDKCIMALLFLIVIGVIAIIIVKL 436
>TC209508 similar to UP|NS13_ARATH (Q9LRP1) Novel plant SNARE 13 (AtNPSN13),
partial (29%)
Length = 510
Score = 132 bits (331), Expect = 1e-31
Identities = 65/79 (82%), Positives = 71/79 (89%)
Frame = +2
Query: 1 MASNLKMTPQLEQIHGEIRDIFRALANGFQKLDKIKDSNRQSTQLEELTGKMRDCKRLIK 60
MASNL+M+PQ+EQIHGEIRD FRALANGFQKLDKIKD+NRQS QLEELT KMR+CKRLIK
Sbjct: 272 MASNLQMSPQVEQIHGEIRDNFRALANGFQKLDKIKDANRQSNQLEELTDKMRECKRLIK 451
Query: 61 DFDREIKDEGAGNPEEVNK 79
+FDREIKDE P EVNK
Sbjct: 452 EFDREIKDEEGRXPPEVNK 508
>BI974298
Length = 420
Score = 115 bits (287), Expect = 2e-26
Identities = 55/65 (84%), Positives = 62/65 (94%)
Frame = +1
Query: 1 MASNLKMTPQLEQIHGEIRDIFRALANGFQKLDKIKDSNRQSTQLEELTGKMRDCKRLIK 60
MASNL+M+PQ+EQIHGEIRD FRALANGFQKLDKIKD+NRQS QLEELT KMR+CKRLIK
Sbjct: 226 MASNLQMSPQVEQIHGEIRDNFRALANGFQKLDKIKDANRQSNQLEELTDKMRECKRLIK 405
Query: 61 DFDRE 65
++DRE
Sbjct: 406 EYDRE 420
>AW830126 similar to GP|20197215|gb| expressed protein {Arabidopsis
thaliana}, partial (26%)
Length = 345
Score = 87.8 bits (216), Expect = 3e-18
Identities = 41/61 (67%), Positives = 52/61 (85%)
Frame = +3
Query: 7 MTPQLEQIHGEIRDIFRALANGFQKLDKIKDSNRQSTQLEELTGKMRDCKRLIKDFDREI 66
++ L +I G I D FRAL+NGFQKL+KIKDSNRQS QLE+LT K+R+CKRLIK+FD+E+
Sbjct: 144 ISEDLAEIDGHIADNFRALSNGFQKLEKIKDSNRQSRQLEDLTEKLRECKRLIKEFDKEV 323
Query: 67 K 67
K
Sbjct: 324 K 326
>AI416848 similar to GP|20197215|gb| expressed protein {Arabidopsis
thaliana}, partial (21%)
Length = 311
Score = 69.7 bits (169), Expect = 9e-13
Identities = 33/50 (66%), Positives = 41/50 (82%)
Frame = +2
Query: 7 MTPQLEQIHGEIRDIFRALANGFQKLDKIKDSNRQSTQLEELTGKMRDCK 56
++ L +I G I D FRAL+NGFQKL+KIKDSNRQS QLE+LT K+R+CK
Sbjct: 62 ISEDLAEIDGHIADNFRALSNGFQKLEKIKDSNRQSRQLEDLTEKLRECK 211
>BI971096 weakly similar to PIR|C96522|C965 F11A17.20 [imported] -
Arabidopsis thaliana, partial (21%)
Length = 597
Score = 43.9 bits (102), Expect = 5e-05
Identities = 22/33 (66%), Positives = 23/33 (69%)
Frame = -2
Query: 174 VKEIGRQVATDKCIMLFLFLIVCGVIAIIVVKV 206
VKEIG QV T CIML L CGVIA IVVK+
Sbjct: 596 VKEIGTQVVTXXCIMLLLXXXXCGVIASIVVKI 498
>TC228521
Length = 1207
Score = 32.3 bits (72), Expect = 0.15
Identities = 27/107 (25%), Positives = 48/107 (44%), Gaps = 14/107 (13%)
Frame = +2
Query: 33 DKIKDSNRQSTQLEELTGKMRDCKRL-------IKDFDREIKDE------GAGNPEEVNK 79
+++ + ++EEL GK++ K L +++ +E+ DE N E +N+
Sbjct: 149 NRLDAKQKLEMEIEELKGKLQVMKHLGDEDDAAVQNKTKEMNDELQEKVDNLENMEAMNQ 328
Query: 80 QL-NDEKQSMTEKNSTAEGNVQLASEMSNQELVNAGMKTMDETDQAI 125
L E+QS E + ++ +M N G+K M E DQ I
Sbjct: 329 TLIVKERQSNDELQEARKELIKGLDDMLNAPRTKIGLKRMGELDQKI 469
>TC224492 similar to UP|Q9VJD4 (Q9VJD4) CG5094-PA (LD24721p), partial (6%)
Length = 525
Score = 31.2 bits (69), Expect = 0.35
Identities = 23/78 (29%), Positives = 36/78 (45%), Gaps = 9/78 (11%)
Frame = +1
Query: 61 DFDREIKDEGAGNPEEVNK--QLNDEKQSMTE-------KNSTAEGNVQLASEMSNQELV 111
D E D GAG PEE N+ Q + EKQ+ E + ++E N ++ E S Q+ +
Sbjct: 238 DLAGEGDDSGAGIPEEQNRTEQSHTEKQTEQEPEHDAPRSSESSENNALISEEESRQKAL 417
Query: 112 NAGMKTMDETDQAIERSK 129
N + E ++ K
Sbjct: 418 NQANEAKVEGNKLFVEGK 471
>TC229683 similar to GB|AAM62553.1|21553460|AY085322 snap25a {Arabidopsis
thaliana;} , partial (66%)
Length = 902
Score = 30.8 bits (68), Expect = 0.45
Identities = 19/73 (26%), Positives = 33/73 (45%)
Frame = +2
Query: 106 SNQELVNAGMKTMDETDQAIERSKQVVHQTIEVGTQTASTLKGQTEQMGRIVNELDSIQF 165
S QEL + + +ET ++ ++ E TQT TL Q EQ+ R + I
Sbjct: 14 SVQELESYAVXXAEETTNSVTNCLKIAEXIREEATQTLVTLHQQGEQITRSHHVAADIDH 193
Query: 166 SIKKASQLVKEIG 178
+ + +L+ +G
Sbjct: 194 DLTRGEKLLGSLG 232
>TC220878 weakly similar to UP|Q84TG3 (Q84TG3) At2g35930, partial (22%)
Length = 923
Score = 30.4 bits (67), Expect = 0.59
Identities = 24/112 (21%), Positives = 54/112 (47%)
Frame = +3
Query: 37 DSNRQSTQLEELTGKMRDCKRLIKDFDREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAE 96
DS+ + ++ + G+ D + ++ + N + N+ + KQ + K+STA
Sbjct: 585 DSSNNNNNIKRMVGENFD---FLNSLTWALQLQTKNNVKLTNEAMPILKQVIEAKDSTAL 755
Query: 97 GNVQLASEMSNQELVNAGMKTMDETDQAIERSKQVVHQTIEVGTQTASTLKG 148
GN++L ++V + MK + + QA++ + V+ +T +G ++G
Sbjct: 756 GNLKL----EFFKVVVSVMKNRELSQQAVKSALHVLIETCPLGRNRMKIVEG 899
>TC232996 similar to UP|Q8S3B4 (Q8S3B4) NADH-ubiquinone oxidoreductase
(Fragment), partial (17%)
Length = 465
Score = 29.6 bits (65), Expect = 1.0
Identities = 14/24 (58%), Positives = 18/24 (74%)
Frame = +1
Query: 3 SNLKMTPQLEQIHGEIRDIFRALA 26
++L+M+P QIHGEI D F ALA
Sbjct: 232 TSLQMSPHAHQIHGEICDHF*ALA 303
>TC214818 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat shock
cognate protein 70, partial (68%)
Length = 1554
Score = 29.3 bits (64), Expect = 1.3
Identities = 21/106 (19%), Positives = 46/106 (42%), Gaps = 16/106 (15%)
Frame = +2
Query: 53 RDCKRLIKDFDREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAEGNVQLAS--------- 103
R +++F R+ K + +GNP + + +++ +STA+ +++ S
Sbjct: 113 RMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEIDSLYEGIDFYS 292
Query: 104 -------EMSNQELVNAGMKTMDETDQAIERSKQVVHQTIEVGTQT 142
E N +L M+ +++ + + K+ VH + VG T
Sbjct: 293 TITRARFEELNMDLFRKCMEPVEKCLRDAKMDKRTVHDVVLVGGST 430
>TC209651 weakly similar to UP|Q9C887 (Q9C887) Myosin-like protein;
97843-94399, partial (51%)
Length = 1013
Score = 29.3 bits (64), Expect = 1.3
Identities = 22/71 (30%), Positives = 35/71 (48%)
Frame = +3
Query: 46 EELTGKMRDCKRLIKDFDREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAEGNVQLASEM 105
EEL + + +RL+ D R I D A + + L K+ + N A G+++ E
Sbjct: 207 EELEFQHAEMRRLVADNRRLIDDRMA-----LQRDLGAAKEELHRMN-LAIGDIRAEHEA 368
Query: 106 SNQELVNAGMK 116
++ELV GMK
Sbjct: 369 HSRELVEKGMK 401
>BE823260
Length = 578
Score = 29.3 bits (64), Expect = 1.3
Identities = 20/75 (26%), Positives = 39/75 (51%), Gaps = 1/75 (1%)
Frame = -2
Query: 40 RQSTQLEELTGKMRDCKRLIKDFDREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAEGNV 99
+ ++EEL GK++ K L + D ++++ EE+N +L ++ ++ +N A +
Sbjct: 556 KXXXEIEELKGKLQVMKHLXDEDDAAVQNK----TEEMNDELQEKVDNL--ENMEAMNQI 395
Query: 100 QLASE-MSNQELVNA 113
+ E SN EL A
Sbjct: 394 LVVKERQSNDELQEA 350
>TC219027 similar to UP|Q94KD3 (Q94KD3) At1g71270/F3I17_8, partial (14%)
Length = 541
Score = 28.9 bits (63), Expect = 1.7
Identities = 25/97 (25%), Positives = 48/97 (48%), Gaps = 2/97 (2%)
Frame = +1
Query: 80 QLNDEKQSMTEKNSTAEGNVQLASEMSNQELVNAGMKTMDETDQAIERSKQ--VVHQTIE 137
+ ND ++++ + + G++ L + S+ ++ G+ +Q +E K VV +
Sbjct: 223 ETNDAQKNVFDLGAFV-GDLTLEDDPSSDDISLEGL------EQELEECKNNDVVANILS 381
Query: 138 VGTQTASTLKGQTEQMGRIVNELDSIQFSIKKASQLV 174
GT+ KG + ++ ELDSIQ IK++ LV
Sbjct: 382 KGTKLRDYTKGVENDLRKV--ELDSIQDYIKESDNLV 486
>BU547613 similar to GP|15528811|dbj P0431G06.18 {Oryza sativa (japonica
cultivar-group)}, partial (13%)
Length = 632
Score = 28.5 bits (62), Expect = 2.2
Identities = 17/69 (24%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = -1
Query: 63 DREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAEGNVQLASEMS--NQELVNAGMKTMDE 120
D+E K + +E+N N E + S + GN+ + +E S Q++V M++M +
Sbjct: 527 DKESKFSSS-KEKEINSDRNTEATRFGQVFSASLGNINMEAEQSAMQQQIVTMYMRSMQQ 351
Query: 121 TDQAIERSK 129
+++ + K
Sbjct: 350 FTESLAKMK 324
>TC225329 similar to UP|ATPX_SPIOL (P31853) ATP synthase B' chain,
chloroplast precursor (Subunit II) , partial (67%)
Length = 1213
Score = 28.1 bits (61), Expect = 2.9
Identities = 20/97 (20%), Positives = 42/97 (42%), Gaps = 9/97 (9%)
Frame = +3
Query: 30 QKLDKIKDSNRQSTQLEELTGKMRDCKR---------LIKDFDREIKDEGAGNPEEVNKQ 80
+KL +KD++ + QLEE + R + K+ E++ + A ++V +
Sbjct: 486 EKLSSVKDTSEEVKQLEEKANAVMAAARAEIAAALNTMKKETQAEVEQKIAEGRKKVEAE 665
Query: 81 LNDEKQSMTEKNSTAEGNVQLASEMSNQELVNAGMKT 117
L + S+ + ++ +QE+VN + T
Sbjct: 666 LQEALSSLENQKEETIKSLDSQIAALSQEIVNKVLPT 776
>TC220324 similar to GB|AAP13360.1|30023654|BT006252 At4g30480 {Arabidopsis
thaliana;} , partial (17%)
Length = 589
Score = 27.7 bits (60), Expect = 3.8
Identities = 25/88 (28%), Positives = 39/88 (43%), Gaps = 18/88 (20%)
Frame = +1
Query: 68 DEGAGNPEEVN------KQLNDEKQSMTEKNSTAE--------GNVQLASEMSNQELVN- 112
D GAG+PEE N + N +QS TEK+ + + Q N L+N
Sbjct: 313 DGGAGSPEEQNHIEQSHTEQNHTEQSHTEKHQQQQQTEQEPEHDDPQSTESSENNALINE 492
Query: 113 --AGMKTMDETDQA-IERSKQVVHQTIE 137
+ K +D+ ++A +E +K V E
Sbjct: 493 EESRQKALDQANEAKVEGNKLFVEGKYE 576
>TC205607 homologue to UP|Q6YIV8 (Q6YIV8) Cytochrome b (Fragment), partial
(5%)
Length = 686
Score = 26.6 bits (57), Expect = 8.5
Identities = 19/62 (30%), Positives = 28/62 (44%)
Frame = +2
Query: 56 KRLIKDFDREIKDEGAGNPEEVNKQLNDEKQSMTEKNSTAEGNVQLASEMSNQELVNAGM 115
K I D E KD+G G PE K ++ +S K T L + +S + + +G
Sbjct: 83 KSKIADTSIESKDDGTGXPEPPAKXKHEAPKSGKSKQET------LKTALSKVKHLKSGG 244
Query: 116 KT 117
KT
Sbjct: 245 KT 250
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.314 0.130 0.339
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,200,059
Number of Sequences: 63676
Number of extensions: 42105
Number of successful extensions: 244
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 244
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 244
length of query: 206
length of database: 12,639,632
effective HSP length: 93
effective length of query: 113
effective length of database: 6,717,764
effective search space: 759107332
effective search space used: 759107332
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146862.25