BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.17 + phase: 0
(310 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC231811 similar to UP|TRUA_STRMU (Q8DWH1) tRNA pseudouridine sy... 310 5e-85
BU764496 226 8e-66
TC210139 weakly similar to UP|TRA1_CLOPE (Q8XLQ0) tRNA pseudouri... 144 4e-35
AI416818 92 2e-19
TC209654 similar to UP|Q6NKP7 (Q6NKP7) At1g09800, partial (47%) 72 4e-13
TC233370 weakly similar to UP|Q7Q242 (Q7Q242) AgCP8994 (Fragment... 43 2e-04
TC222548 similar to GB|AAN18175.1|23308411|BT000606 At1g76120/T2... 37 0.015
BM270316 36 0.019
CO985253 35 0.043
TC231156 similar to GB|AAP37871.1|30725698|BT008512 At5g35400 {A... 34 0.074
BI787676 29 2.4
TC221505 similar to UP|Q9LMI7 (Q9LMI7) T2D23.2 protein, partial ... 29 3.1
TC207118 homologue to UP|Q6T2Z6 (Q6T2Z6) Cyclin d3, complete 28 5.3
TC233169 28 5.3
TC211738 similar to UP|Q9LT41 (Q9LT41) RCD1, partial (42%) 28 6.9
BI973628 28 6.9
TC218200 similar to UP|Q8LPQ5 (Q8LPQ5) AT3g18290/MIE15_8, partia... 28 6.9
TC208948 similar to UP|Q93ZL4 (Q93ZL4) At3g07700/F17A17.4, parti... 28 6.9
CD414604 similar to GP|15983801|gb| At3g07700/F17A17.4 {Arabidop... 28 6.9
TC211050 UP|Q7QR02 (Q7QR02) GLP_442_5166_5819, partial (5%) 27 9.0
>TC231811 similar to UP|TRUA_STRMU (Q8DWH1) tRNA pseudouridine synthase A
(Pseudouridylate synthase I) (Pseudouridine synthase I)
(Uracil hydrolyase) , partial (15%)
Length = 640
Score = 310 bits (794), Expect = 5e-85
Identities = 168/244 (68%), Positives = 179/244 (72%), Gaps = 5/244 (2%)
Frame = +3
Query: 66 ATKLQRKDLQLVGASRTDAGVHAWGQVAHFLTPFNYDNLDSVHAALNGLLPSDIRVREIS 125
ATKLQRKDL LVGASRTDAGVHAWGQVAHF TPFNYDNL+ +HAALNGLLP+DIR+REIS
Sbjct: 9 ATKLQRKDLCLVGASRTDAGVHAWGQVAHFFTPFNYDNLEQLHAALNGLLPTDIRIREIS 188
Query: 126 PASAEFHARFSVKS----KIYHYKIYSDTIMDPFQRHFAYHNMYKLNSAAMREAARYFIG 181
PASAEFHARFS K+ K+ H + S I P H IG
Sbjct: 189 PASAEFHARFSAKTLL*EKLQHILLAS-MISLPLPMHPT------------------MIG 311
Query: 182 KHDFTAFENASHNDRIPDPVKHIFPIDVKEMGALLQLEVEGSGFLYRQVRNMVALLLQIG 241
+PDPVKH+F D+KEMGALLQLEVEGSGFLYRQVRNMVALLLQIG
Sbjct: 312 ---------------VPDPVKHVFRFDLKEMGALLQLEVEGSGFLYRQVRNMVALLLQIG 446
Query: 242 KEATPPDIVPHILASRDRKELAKYCYYLPPHGLCLVSINYNESHLLPPPGC-PAKSFGMH 300
+EA PPDIVPHILASRDRKELAKY PPHGLCLVSINYNESHLLPPPGC PA SFG H
Sbjct: 447 REAIPPDIVPHILASRDRKELAKYSLSAPPHGLCLVSINYNESHLLPPPGCPPANSFGRH 626
Query: 301 HTIR 304
HTIR
Sbjct: 627 HTIR 638
>BU764496
Length = 438
Score = 226 bits (577), Expect(2) = 8e-66
Identities = 103/125 (82%), Positives = 115/125 (91%)
Frame = +1
Query: 92 VAHFLTPFNYDNLDSVHAALNGLLPSDIRVREISPASAEFHARFSVKSKIYHYKIYSDTI 151
VAHF TPFNYDNL+ +HAALNGLLP+DIR+REISPASAEFHARFS KS IYHYKIY+D I
Sbjct: 64 VAHFFTPFNYDNLEQLHAALNGLLPTDIRIREISPASAEFHARFSAKSYIYHYKIYNDAI 243
Query: 152 MDPFQRHFAYHNMYKLNSAAMREAARYFIGKHDFTAFENASHNDRIPDPVKHIFPIDVKE 211
MDPFQRHFAYH++YKLNSA MREAA YF+GKHDF+AF NASHNDR+PDPVKH+F D+KE
Sbjct: 244 MDPFQRHFAYHSVYKLNSAVMREAATYFVGKHDFSAFANASHNDRVPDPVKHVFRFDLKE 423
Query: 212 MGALL 216
MGALL
Sbjct: 424 MGALL 438
Score = 42.0 bits (97), Expect(2) = 8e-66
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = +3
Query: 70 QRKDLQLVGASRTDAGVHAWG 90
Q KDL LVGASRTDAGVHAWG
Sbjct: 3 QTKDLCLVGASRTDAGVHAWG 65
>TC210139 weakly similar to UP|TRA1_CLOPE (Q8XLQ0) tRNA pseudouridine
synthase A 1 (Pseudouridylate synthase I 1)
(Pseudouridine synthase I 1) (Uracil hydrolyase 1) ,
partial (9%)
Length = 522
Score = 144 bits (364), Expect = 4e-35
Identities = 79/124 (63%), Positives = 80/124 (63%), Gaps = 36/124 (29%)
Frame = +3
Query: 223 SGFLYRQVRNMV-----------------------------------ALLLQIGKEATPP 247
SGFLYRQVRNMV ALLLQIG+EA PP
Sbjct: 6 SGFLYRQVRNMVNSLVPK*SKILLCSCKITV*LFHFCNEHLCLLF*VALLLQIGREAIPP 185
Query: 248 DIVPHILASRDRKELAKYCYYLPPHGLCLVSINYNESHLLPPPGC-PAKSFGMHHTIRKC 306
DIVPHILASRDRKELAKY PPHGLCLVSINYNESHLLPPPGC PA SFG HHTIRKC
Sbjct: 186 DIVPHILASRDRKELAKYSLSAPPHGLCLVSINYNESHLLPPPGCPPANSFGRHHTIRKC 365
Query: 307 KVVF 310
KV F
Sbjct: 366 KVPF 377
>AI416818
Length = 369
Score = 92.4 bits (228), Expect = 2e-19
Identities = 46/58 (79%), Positives = 46/58 (79%), Gaps = 1/58 (1%)
Frame = +2
Query: 254 LASRDRKELAKYCYYLPPHGLCLVSINYNESHLLPPP-GCPAKSFGMHHTIRKCKVVF 310
LASRDRKELA Y PPHGLCLVSINYNESHLLPPP PA SFG H TIRKCKV F
Sbjct: 2 LASRDRKELAPYSLPAPPHGLCLVSINYNESHLLPPPESPPANSFGRHQTIRKCKVPF 175
>TC209654 similar to UP|Q6NKP7 (Q6NKP7) At1g09800, partial (47%)
Length = 759
Score = 71.6 bits (174), Expect = 4e-13
Identities = 55/178 (30%), Positives = 89/178 (49%), Gaps = 17/178 (9%)
Frame = +1
Query: 31 KWRLLLSYDGTRYAGWQYQESPPTVQCAVEKALIRATKLQRKDLQLVGASRTDAGVHAWG 90
++ + + Y GT ++G Q Q + TV A+E+A +K + + + +SRTDAGVHA
Sbjct: 235 RYLVAIEYIGTLFSGSQKQLNVRTVVGALEEAF---SKFVGQPVSVSCSSRTDAGVHALS 405
Query: 91 QVAHF-------------LTPFNYDNLDSVHAALNGLLP---SDIRVREISPASAEFHAR 134
V H L P V A+N L SD+ V ++ ++FHAR
Sbjct: 406 NVCHVDIQRISKRRPGEVLPPHEPA---VVGRAVNHFLQKHDSDLMVIDVRCVPSDFHAR 576
Query: 135 FSVKSKIYHYKIYSDTI-MDPFQRHFAYHNMYKLNSAAMREAARYFIGKHDFTAFENA 191
F + + Y Y++ S + F++ A+H +L+ AM+EA R +G HDF++F A
Sbjct: 577 FKAQERTYFYRLLSGPEPLSTFEKDRAWHVPEELSLPAMQEACRVLVGCHDFSSFRAA 750
>TC233370 weakly similar to UP|Q7Q242 (Q7Q242) AgCP8994 (Fragment), partial
(17%)
Length = 868
Score = 42.7 bits (99), Expect = 2e-04
Identities = 20/46 (43%), Positives = 28/46 (60%)
Frame = +1
Query: 111 LNGLLPSDIRVREISPASAEFHARFSVKSKIYHYKIYSDTIMDPFQ 156
LN +LP+DIR+ PA +FHARFS S+ Y Y + + + P Q
Sbjct: 88 LNRVLPNDIRIMGWCPAPVDFHARFSCLSREYKYFFWKENLNIPVQ 225
Score = 36.6 bits (83), Expect = 0.015
Identities = 15/37 (40%), Positives = 25/37 (67%)
Frame = +2
Query: 218 LEVEGSGFLYRQVRNMVALLLQIGKEATPPDIVPHIL 254
+++ GS FL+ QVR MVA+L +GK P+++ +L
Sbjct: 245 IKIRGSAFLWHQVRCMVAVLFMVGKGLESPNVIDMLL 355
>TC222548 similar to GB|AAN18175.1|23308411|BT000606 At1g76120/T23E18_5
{Arabidopsis thaliana;} , partial (34%)
Length = 613
Score = 36.6 bits (83), Expect = 0.015
Identities = 34/122 (27%), Positives = 54/122 (43%), Gaps = 6/122 (4%)
Frame = +1
Query: 31 KWRLLLSYDGTRYAGWQYQESPPTVQCAVEKALIRATKLQRKDLQL------VGASRTDA 84
K + +Y G Y G Q T++ +E+AL + + D + ++RTD
Sbjct: 73 KVAIFFAYCGVGYQGMQKNPGAKTIEGDLEEALYVSGAVPLYDRGVPKRYDWARSARTDK 252
Query: 85 GVHAWGQVAHFLTPFNYDNLDSVHAALNGLLPSDIRVREISPASAEFHARFSVKSKIYHY 144
GV A GQV ++ Y + + LN LPS IR+ +A F+A+ + Y Y
Sbjct: 253 GVSAVGQV---VSGRFYIDPPGLVDRLNSNLPSQIRIFGYKRVTASFNAKKFCDRRRYVY 423
Query: 145 KI 146
I
Sbjct: 424 LI 429
>BM270316
Length = 414
Score = 36.2 bits (82), Expect = 0.019
Identities = 22/65 (33%), Positives = 37/65 (56%)
Frame = +2
Query: 24 SPNQDGYKWRLLLSYDGTRYAGWQYQESPPTVQCAVEKALIRATKLQRKDLQLVGASRTD 83
+P ++ + + Y GTR++G Q Q + TV +E+A +K + + + +SRTD
Sbjct: 224 NPRNGIQRYLVAIEYIGTRFSGSQKQLNVRTVVGVLEEAF---SKFVGQPVSVSCSSRTD 394
Query: 84 AGVHA 88
AGVHA
Sbjct: 395 AGVHA 409
>CO985253
Length = 826
Score = 35.0 bits (79), Expect = 0.043
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = -2
Query: 218 LEVEGSGFLYRQVRNMVALLLQIGKEATPPDIVPHIL 254
+++ GS FL+ QVR MV +L +GK P+++ +L
Sbjct: 780 IKIRGSAFLWHQVRCMVVVLFMVGKGLESPNVIDMLL 670
>TC231156 similar to GB|AAP37871.1|30725698|BT008512 At5g35400 {Arabidopsis
thaliana;} , partial (28%)
Length = 739
Score = 34.3 bits (77), Expect = 0.074
Identities = 30/108 (27%), Positives = 47/108 (42%), Gaps = 9/108 (8%)
Frame = +1
Query: 185 FTAFENASHNDRIPDP--VKHIFPIDVK-------EMGALLQLEVEGSGFLYRQVRNMVA 235
F AS ND P V H ++VK E ++ +E+ + FL + VR +VA
Sbjct: 151 FARDTKASRNDGPPTECFVYHARAMEVKLPATDHGEETKVMCVELVANRFLRKMVRVLVA 330
Query: 236 LLLQIGKEATPPDIVPHILASRDRKELAKYCYYLPPHGLCLVSINYNE 283
++ D + ++ + R+ A PP GLCLV + Y E
Sbjct: 331 TSIREAAAGAEDDALIKLMDATCRRATAPPA---PPDGLCLVDVGYTE 465
>BI787676
Length = 421
Score = 29.3 bits (64), Expect = 2.4
Identities = 23/62 (37%), Positives = 31/62 (49%), Gaps = 3/62 (4%)
Frame = -3
Query: 242 KEATPPDIVPHILASRDRKELAKYCYYL-PPHGLCLVSIN--YNESHLLPPPGCPAKSFG 298
K++T PD HI R RK L + PPH L+S N + H+L P P +S G
Sbjct: 413 KQSTFPD--SHI-RRRSRKRLRSIHKLVGPPHRTILISANVKFIPEHVLYHPAKPNESIG 243
Query: 299 MH 300
+H
Sbjct: 242 LH 237
>TC221505 similar to UP|Q9LMI7 (Q9LMI7) T2D23.2 protein, partial (9%)
Length = 613
Score = 28.9 bits (63), Expect = 3.1
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = +2
Query: 255 ASRDRKELAKYCYYLPPHGLCLVS 278
AS RKE+ K C L PH L LVS
Sbjct: 233 ASAPRKEVPKLCAELRPHALALVS 304
>TC207118 homologue to UP|Q6T2Z6 (Q6T2Z6) Cyclin d3, complete
Length = 1763
Score = 28.1 bits (61), Expect = 5.3
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = -3
Query: 250 VPHILASRDRKELAKYCYYLPPH 272
+PH A RD+ A YCYY H
Sbjct: 561 LPHDEAERDKSPTAHYCYYYCDH 493
>TC233169
Length = 649
Score = 28.1 bits (61), Expect = 5.3
Identities = 13/31 (41%), Positives = 21/31 (66%)
Frame = +2
Query: 228 RQVRNMVALLLQIGKEATPPDIVPHILASRD 258
RQ+R +ALLLQ G++AT V H++ ++
Sbjct: 155 RQMRRDIALLLQSGQDATARIRVEHVMREQN 247
>TC211738 similar to UP|Q9LT41 (Q9LT41) RCD1, partial (42%)
Length = 571
Score = 27.7 bits (60), Expect = 6.9
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +3
Query: 10 SSVVTPIPSPTSLLSPNQDGYKWRLLLSYDGTRYAGWQY 48
S+ TPI T ++ + G +R+LL Y G A WQ+
Sbjct: 207 SNSATPISEKTLSMNSPRRGSYFRILLRYCGILLAPWQH 323
>BI973628
Length = 402
Score = 27.7 bits (60), Expect = 6.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 274 LCLVSINYNESHLLPPPGCPAKSF 297
L +++ YN+SH+L PG PA +
Sbjct: 189 LIIIN*GYNDSHILENPGAPAHPY 118
>TC218200 similar to UP|Q8LPQ5 (Q8LPQ5) AT3g18290/MIE15_8, partial (25%)
Length = 1175
Score = 27.7 bits (60), Expect = 6.9
Identities = 13/61 (21%), Positives = 28/61 (45%)
Frame = +1
Query: 145 KIYSDTIMDPFQRHFAYHNMYKLNSAAMREAARYFIGKHDFTAFENASHNDRIPDPVKHI 204
K+Y D+ +DP ++ + N+ A ++ + + + + E + DP KH+
Sbjct: 70 KVYRDSTLDPRRKAYLVQNLMTSRWIAAQQKSPKALSEGSSNSVEIEGLSPSFRDPGKHV 249
Query: 205 F 205
F
Sbjct: 250 F 252
>TC208948 similar to UP|Q93ZL4 (Q93ZL4) At3g07700/F17A17.4, partial (33%)
Length = 846
Score = 27.7 bits (60), Expect = 6.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2
Query: 131 FHARFSVKSKIYHYKIYSDTI 151
FH+ FS K KIY Y++Y + I
Sbjct: 779 FHSYFSHKHKIYEYELYRNMI 841
>CD414604 similar to GP|15983801|gb| At3g07700/F17A17.4 {Arabidopsis
thaliana}, partial (8%)
Length = 635
Score = 27.7 bits (60), Expect = 6.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -1
Query: 131 FHARFSVKSKIYHYKIYSDTI 151
FH+ FS K KIY Y++Y + I
Sbjct: 347 FHSYFSHKHKIYEYELYRNMI 285
>TC211050 UP|Q7QR02 (Q7QR02) GLP_442_5166_5819, partial (5%)
Length = 512
Score = 27.3 bits (59), Expect = 9.0
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +1
Query: 32 WRLLLSYDGTRYAGWQYQESPPTVQC 57
WR ++ TR+AGW Y P ++C
Sbjct: 364 WRF*IAKLHTRFAGWPYSTLPQFLKC 441
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.137 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,437,487
Number of Sequences: 63676
Number of extensions: 262338
Number of successful extensions: 1372
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 1361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1366
length of query: 310
length of database: 12,639,632
effective HSP length: 97
effective length of query: 213
effective length of database: 6,463,060
effective search space: 1376631780
effective search space used: 1376631780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146862.17