BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146856.10 + phase: 0
(1260 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CD415908 206 5e-53
TC234271 homologue to UP|Q25639 (Q25639) Allergen (Fragment), pa... 34 0.36
BU927253 similar to GP|14194119|gb| At1g01540/F22L4_6 {Arabidops... 32 1.4
BM885457 similar to PIR|S56655|S56 lipoxygenase (EC 1.13.11.12) ... 32 2.3
TC214559 32 2.3
BU578205 similar to GP|5081555|gb|A PHAP2A protein {Petunia x hy... 32 2.3
TC219871 28 2.7
BM519608 31 3.0
TC214075 similar to UP|P93014 (P93014) 30S ribosomal protein S5,... 31 3.0
BU578284 31 3.9
TC213821 weakly similar to UP|O81373 (O81373) Cotton fiber expre... 31 3.9
TC208627 weakly similar to UP|Q9FGF4 (Q9FGF4) Arabidopsis thalia... 31 3.9
TC220766 homologue to UP|Q9Q5L3 (Q9Q5L3) EBNA-2, partial (4%) 30 5.1
TC223535 weakly similar to UP|Q6TY42 (Q6TY42) 60kDa glycoprotein... 30 6.7
TC220354 similar to UP|Q6ZI84 (Q6ZI84) RNA methylase-like protei... 30 8.8
TC214486 30 8.8
TC205480 weakly similar to UP|Q08883 (Q08883) Narbonin, partial ... 30 8.8
TC205764 homologue to UP|ERD2_PETHY (Q9ZTN2) ER lumen protein re... 30 8.8
TC218565 30 8.8
>CD415908
Length = 596
Score = 206 bits (524), Expect = 5e-53
Identities = 113/171 (66%), Positives = 127/171 (74%)
Frame = -2
Query: 767 VAVGVAWQVRDLLQHEDTRKAIFRSQPLEMLFFSLKMVSEVSIEHCGQYEYIDISPVLAY 826
V VGV WQVRDLL EDTRKAI S LEMLF SLK++SEVSI HCG +EYIDI PVLAY
Sbjct: 595 VPVGVTWQVRDLLLQEDTRKAILESPSLEMLFVSLKVISEVSIVHCGHHEYIDICPVLAY 416
Query: 827 MALALQMTVDNVGTSSEKNGDSKGKKTKIDSSTLLSEAILDLTIEHVLNCLEKLFGSDDT 886
+A ALQMTVDNVG SS N D+K KKTKIDSSTLLSE IL+LTIEHVLNCLEKLFG+
Sbjct: 415 VAFALQMTVDNVGRSSIHNSDTKRKKTKIDSSTLLSENILELTIEHVLNCLEKLFGAGGI 236
Query: 887 VQDHNVDSHNLESTTGKNRSSKKRKRTGKNQNSTKRRRLSLTNAGCPSNEG 937
+++H+ DS NL+ T RT +NQNS R RLS T+A PSN G
Sbjct: 235 MKNHDGDSCNLQPT----------NRTNQNQNSRPRPRLSPTDACRPSNRG 113
>TC234271 homologue to UP|Q25639 (Q25639) Allergen (Fragment), partial (7%)
Length = 568
Score = 34.3 bits (77), Expect = 0.36
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = +3
Query: 41 PSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQTPPTATLRRSSRKNTTATT 96
PSS + S PS S T+ + +S + +TPP T SSR+ +T +T
Sbjct: 339 PSSTTVPSASPSTARSRTSAASTCPTCTSRTSSSSTRTPPPTTTTSSSRRTSTTST 506
>BU927253 similar to GP|14194119|gb| At1g01540/F22L4_6 {Arabidopsis
thaliana}, partial (13%)
Length = 309
Score = 32.3 bits (72), Expect = 1.4
Identities = 29/86 (33%), Positives = 42/86 (48%)
Frame = +3
Query: 7 SSLNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLN 66
SS SSP S A+ S+ SS + S PSS+ S+ PP+ + TTTI++
Sbjct: 75 SSTQSSPNALPSSASASGSSSASSSDPS---SSSPSSSSPSASPPAAATTTTTTIRTPAP 245
Query: 67 LLELNNSENPQTPPTATLRRSSRKNT 92
S PQ+P ++SSR +T
Sbjct: 246 ATPPPPSPLPQSP-----KKSSRSST 308
>BM885457 similar to PIR|S56655|S56 lipoxygenase (EC 1.13.11.12) loxG -
garden pea, partial (16%)
Length = 421
Score = 31.6 bits (70), Expect = 2.3
Identities = 16/43 (37%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = -1
Query: 464 HSHEVLKVLCPRLGHLML-DNVLTVQVAMAELLLHLNDVQNFQ 505
HSH VLC L HL+L +VL + ++ + + HL D+ Q
Sbjct: 394 HSHRSFHVLCALLFHLLLKSSVLLLYTSLVDPVFHLEDMSCLQ 266
>TC214559
Length = 762
Score = 31.6 bits (70), Expect = 2.3
Identities = 31/105 (29%), Positives = 46/105 (43%)
Frame = +1
Query: 379 SKEDKDKLLDKQFFLLEKLLVDDCPEVRTIAVEGSCRVLHLFWEIIPSPIITKMLTKVIS 438
S E+ KL ++ LL+ L CP +R + IPS L +
Sbjct: 61 SHENAHKLSEQPTRLLQNLNCVGCPNIRKV--------------FIPSTAHCSRLLFLNL 198
Query: 439 DMSHDVCNEVRLSTLSGIIYLLDNPHSHEVLKVLCPRLGHLMLDN 483
+S ++ EV ++ L+ L N S EVLK+ CPRL L L +
Sbjct: 199 SLSANL-KEVDVACLNLSWLNLSNCSSLEVLKLECPRLTSLFLQS 330
>BU578205 similar to GP|5081555|gb|A PHAP2A protein {Petunia x hybrida},
partial (6%)
Length = 409
Score = 31.6 bits (70), Expect = 2.3
Identities = 16/49 (32%), Positives = 29/49 (58%)
Frame = -3
Query: 78 TPPTATLRRSSRKNTTATTEPSSQSDEKHKLLEKLQILAHILFLCVSHP 126
TPP+++ SS ++T++++PSS + + LE L +L L +S P
Sbjct: 392 TPPSSSSSSSSSSSSTSSSDPSSMTTAEELELETDPTLLPLLLLIMSSP 246
>TC219871
Length = 1292
Score = 27.7 bits (60), Expect(2) = 2.7
Identities = 13/42 (30%), Positives = 24/42 (56%)
Frame = +3
Query: 1011 FCLKSSFSYAAKILNVILTDSGGSSIMTSKAFTLANNLLDLI 1052
FC K+S + KI + ++S S+++S TL NN+ ++
Sbjct: 1107 FCCKTS*VWEVKIWKLDPSNSAKKSLISSSRLTLRNNMPQIV 1232
Score = 21.9 bits (45), Expect(2) = 2.7
Identities = 9/22 (40%), Positives = 12/22 (53%)
Frame = +2
Query: 956 VLKFMADATAMCFAPHNNGSFL 977
V + D C+A HNN +FL
Sbjct: 1046 VSPLLKDTINCCYAWHNNTTFL 1111
>BM519608
Length = 434
Score = 31.2 bits (69), Expect = 3.0
Identities = 17/39 (43%), Positives = 22/39 (55%)
Frame = +2
Query: 32 LKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLNLLEL 70
L L+HS+ SS +I PS+Y S T I S L+L L
Sbjct: 32 LLLLLHSLSSSSKIIFLFYPSIYHSFTLKIFSLLSLAVL 148
>TC214075 similar to UP|P93014 (P93014) 30S ribosomal protein S5, partial
(76%)
Length = 1140
Score = 31.2 bits (69), Expect = 3.0
Identities = 23/69 (33%), Positives = 28/69 (40%), Gaps = 6/69 (8%)
Frame = +3
Query: 37 HSIKPSSNLISSLPPSLYESITTTIQSFLNLLELNNSENPQT------PPTATLRRSSRK 90
H P + +S PPSL S S +L N NP + PPT RSS
Sbjct: 51 HLHSPPLSTLSPPPPSLSPSPPPPAASLSSLHPHTNPPNPSSSPSNPNPPTTLTHRSSTT 230
Query: 91 NTTATTEPS 99
+T TT S
Sbjct: 231 STRKTTSHS 257
>BU578284
Length = 443
Score = 30.8 bits (68), Expect = 3.9
Identities = 25/93 (26%), Positives = 43/93 (45%), Gaps = 1/93 (1%)
Frame = -2
Query: 9 LNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSFLNLL 68
L P + + T + SS + + S +++S+ +L S++ T+ S L LL
Sbjct: 442 LQLQPPTALGFSVTTKAERISSST*STVAPFTKSYDMLSTT--NLTPSLSKTLSSELILL 269
Query: 69 E-LNNSENPQTPPTATLRRSSRKNTTATTEPSS 100
L NP+ PP +TL+RS + + SS
Sbjct: 268 SRLKTYWNPEHPPVSTLKRSKLPSVDDSLPSSS 170
>TC213821 weakly similar to UP|O81373 (O81373) Cotton fiber expressed protein
1, partial (16%)
Length = 444
Score = 30.8 bits (68), Expect = 3.9
Identities = 36/106 (33%), Positives = 47/106 (43%), Gaps = 11/106 (10%)
Frame = +1
Query: 5 LCSSLNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSF 64
LCS ++SS + T SS +SL S SS+ SSLPP+ S TTT
Sbjct: 160 LCSRISSSTKPL-----TPQPSSSPASLPLTSTSFSTSSSSPSSLPPN---STTTT---- 303
Query: 65 LNLLELNNSENPQTPPTATL-----RRSSRKNTTA------TTEPS 99
L + S +P TP + R S RK +T+ TT PS
Sbjct: 304 -TLRRIPPSSSPPTPSSTPYHPPQSRSSLRKPSTSLPLYKRTTTPS 438
>TC208627 weakly similar to UP|Q9FGF4 (Q9FGF4) Arabidopsis thaliana genomic
DNA, chromosome 5, BAC clone:T12B11, partial (8%)
Length = 1012
Score = 30.8 bits (68), Expect = 3.9
Identities = 37/161 (22%), Positives = 66/161 (40%), Gaps = 2/161 (1%)
Frame = +1
Query: 280 ILFRAWKAAPEDSRSGIEDGFLQDLIEGSIHASSGVFALYIRRVLGGFINQRTVDGVEKL 339
++FR D +S I+ GFL+++I +F + R + R V+ +E +
Sbjct: 250 LIFREVLVGYFDKKSQIKSGFLKEIIRRRPWIGHAIFGFILERCGSAKSDFRRVEALELV 429
Query: 340 LYRLAEPVIFRSLQAANSNVRQNALHLLLDIFPLEDPDASKEDKDKLLDKQFFLLEKLLV 399
+ I +SL NS ++ +ASK+ LDK LL++L+
Sbjct: 430 M------EILKSLSTGNS----------------DEQNASKKILKNSLDKLSHLLKELVT 543
Query: 400 D--DCPEVRTIAVEGSCRVLHLFWEIIPSPIITKMLTKVIS 438
+ P RT + + L EI+ +TK K ++
Sbjct: 544 NMPSKPARRTEVQKFCVKAL----EILSKLNLTKNFVKTLA 654
>TC220766 homologue to UP|Q9Q5L3 (Q9Q5L3) EBNA-2, partial (4%)
Length = 885
Score = 30.4 bits (67), Expect = 5.1
Identities = 25/69 (36%), Positives = 36/69 (51%), Gaps = 2/69 (2%)
Frame = +3
Query: 27 SSKSSLKTLIHSIKPSSNLISSLPPSL--YESITTTIQSFLNLLELNNSENPQTPPTATL 84
SS S TL + KPS + +S PPS Y + T I +L L+ S NP +PP++
Sbjct: 3 SSSPSPTTLSSNPKPSLSSTASDPPSAKPYHPLPTII----TILPLSLS-NPNSPPSSKT 167
Query: 85 RRSSRKNTT 93
R ++ TT
Sbjct: 168RFTTTTTTT 194
>TC223535 weakly similar to UP|Q6TY42 (Q6TY42) 60kDa glycoprotein (Fragment),
partial (24%)
Length = 431
Score = 30.0 bits (66), Expect = 6.7
Identities = 31/103 (30%), Positives = 40/103 (38%), Gaps = 3/103 (2%)
Frame = +1
Query: 5 LCSSLNSSPQEFISLATTLTLKSSKSSLKTLIHSIKPSSNLISSLPPSLYESITTTIQSF 64
L SS +S P +S L+ LI K + LIS PPS + SF
Sbjct: 28 LSSSSSSQPSSILSFR-----------LQNLISESKKMNTLIS--PPSTNRYLCGVPYSF 168
Query: 65 LNLLELNNSEN---PQTPPTATLRRSSRKNTTATTEPSSQSDE 104
L+ L NN+ N P+ T S AT + SDE
Sbjct: 169 LSPLRHNNNTNLNLPRFHSTVATNFLSHSPVRATLSARATSDE 297
>TC220354 similar to UP|Q6ZI84 (Q6ZI84) RNA methylase-like protein, partial
(58%)
Length = 591
Score = 29.6 bits (65), Expect = 8.8
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +3
Query: 71 NNSENPQTPPTATLRRSSRKNTTATT 96
N+SE P+TP TAT R+ N+ TT
Sbjct: 6 NSSEQPRTPKTATFPRNCNGNSLITT 83
>TC214486
Length = 1070
Score = 29.6 bits (65), Expect = 8.8
Identities = 30/106 (28%), Positives = 47/106 (44%)
Frame = +1
Query: 378 ASKEDKDKLLDKQFFLLEKLLVDDCPEVRTIAVEGSCRVLHLFWEIIPSPIITKMLTKVI 437
+S E+ +L ++ LL+ L CP +R + IPS L +
Sbjct: 424 SSPENVLELSEQPIRLLQNLNCVGCPNIRKV--------------FIPSTAHCSRLLFLN 561
Query: 438 SDMSHDVCNEVRLSTLSGIIYLLDNPHSHEVLKVLCPRLGHLMLDN 483
+S ++ EV ++ L+ L N S EVLK+ CPRL L L +
Sbjct: 562 LSLSANL-KEVDVACLNLSWLNLSNCSSLEVLKLECPRLTSLFLQS 696
>TC205480 weakly similar to UP|Q08883 (Q08883) Narbonin, partial (8%)
Length = 1157
Score = 29.6 bits (65), Expect = 8.8
Identities = 21/62 (33%), Positives = 31/62 (49%)
Frame = +3
Query: 378 ASKEDKDKLLDKQFFLLEKLLVDDCPEVRTIAVEGSCRVLHLFWEIIPSPIITKMLTKVI 437
A KED +K+ FFL LV P V+ +V +V+ + IP I K+L+ +
Sbjct: 756 AIKEDWNKVSPPLFFLALNRLVHSWPRVKGASV----KVISDTYAFIPPTWIPKLLSLAV 923
Query: 438 SD 439
SD
Sbjct: 924 SD 929
>TC205764 homologue to UP|ERD2_PETHY (Q9ZTN2) ER lumen protein retaining
receptor (HDEL receptor) (PGP169-12), complete
Length = 1133
Score = 29.6 bits (65), Expect = 8.8
Identities = 28/121 (23%), Positives = 50/121 (41%), Gaps = 1/121 (0%)
Frame = -1
Query: 887 VQDHNVDSHNLESTTGKNRSSKKRKRTGKNQNSTKRRRLS-LTNAGCPSNEGSVHNEPQQ 945
V+D N HNL+ T + + K + +T S +S + +N+ ++ N Q+
Sbjct: 1055 VKDQNALCHNLDLNTFSS*NCKLQSQTN*CFLSIIFLVISTVVFEVARTNK*AIVN--QK 882
Query: 946 VLCKVKMITAVLKFMADATAMCFAPHNNGSFLNNTSKCIQHILSSLNQLYHHKIEFEEED 1005
LC K + + + CF N L N + C + N+ H++ FE+
Sbjct: 881 PLCPDKFVNPLCLLLIVHLDPCFQEQKNKLMLANEAFCCSSSFGNSNRRNQHRVVFEQGQ 702
Query: 1006 K 1006
K
Sbjct: 701 K 699
>TC218565
Length = 626
Score = 29.6 bits (65), Expect = 8.8
Identities = 13/45 (28%), Positives = 25/45 (54%)
Frame = -2
Query: 1040 KAFTLANNLLDLIVSTESCLGSAYASRLVAAAKPWLPDVVLALGS 1084
KAF +LL +++ + L A+ L ++ +PW P + +AL +
Sbjct: 529 KAFNCGRSLLSFVITFSNFLLKLIATLLYSSLQPWSPKMFVALSN 395
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.318 0.134 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,467,385
Number of Sequences: 63676
Number of extensions: 728148
Number of successful extensions: 4244
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 4182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4240
length of query: 1260
length of database: 12,639,632
effective HSP length: 108
effective length of query: 1152
effective length of database: 5,762,624
effective search space: 6638542848
effective search space used: 6638542848
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC146856.10