BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146855.1 + phase: 0 /pseudo
(1356 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG405621 88 2e-17
CA851030 58 3e-08
CA851997 37 4e-04
TC233199 43 8e-04
CA936755 40 0.005
TC206673 40 0.007
CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partia... 39 0.012
BI321779 38 0.026
CO982668 35 0.17
TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment)... 35 0.22
TC222762 35 0.29
BM732560 34 0.50
TC219606 similar to UP|O48722 (O48722) Heme oxygenase 2 precurso... 32 1.4
TC230791 homologue to UP|Q7S6J2 (Q7S6J2) Predicted protein, part... 32 2.5
BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase... 31 3.2
BM891643 weakly similar to GP|9759426|dbj WD-40 repeat protein-l... 31 3.2
AW099854 31 4.2
BI497688 similar to GP|14517500|gb| K16N12.18/K16N12.18 {Arabido... 30 5.5
CD413613 30 5.5
BI968991 30 5.5
>BG405621
Length = 402
Score = 88.2 bits (217), Expect = 2e-17
Identities = 53/132 (40%), Positives = 70/132 (52%), Gaps = 3/132 (2%)
Frame = +2
Query: 229 LQLDPPPWRAPHNSFRFNNSWLIEPELAHLVKDNWSYYPSSNIVTKLSYCIEDMKYWSKA 288
L LD R +F F NSWL+EP L +V WS S+N+++KL+ I + +
Sbjct: 2 LYLDASKRRIFGRNFIFENSWLLEPGLEEIVHFGWSKPLSNNLISKLNC*IVALMK*TNE 181
Query: 289 NH---PHFNQRKQQLKNQIDVMRNTSDASVDPRLIELQNSLANLILQEDVYWRQRSKIFW 345
F+ + + + I+ R D D R E Q L N I QE+ YW+QRSKIFW
Sbjct: 182 GRILKRKFHDQIEACRKDIEASRFFHDPVNDARY*EAQAKLNNFIAQEESYWQQRSKIFW 361
Query: 346 LKDGDKNSKFFH 357
LK GD NSKFFH
Sbjct: 362 LKYGDLNSKFFH 397
>CA851030
Length = 358
Score = 57.8 bits (138), Expect = 3e-08
Identities = 23/56 (41%), Positives = 38/56 (67%)
Frame = +1
Query: 322 LQNSLANLILQEDVYWRQRSKIFWLKDGDKNSKFFHLTASSRRRKNTISKLRHPNG 377
++ SL +L+ QE+ YW+QRS+ W K+ DKN++FFH R+ N+I+K++ G
Sbjct: 139 VEESLDSLLKQEEEYWQQRSRAIWFKERDKNTEFFHRKTLQRKASNSITKIQDDEG 306
>CA851997
Length = 636
Score = 37.0 bits (84), Expect(2) = 4e-04
Identities = 18/44 (40%), Positives = 27/44 (60%)
Frame = +3
Query: 396 GLFQAINGDQQPVITRIQARVTENDNATLTKPFSIDEFKEAVFS 439
GLF NG+ VI ++++ +T +DN L +PF E KEA F+
Sbjct: 177 GLFADGNGNHGVVIDKVRSIITTDDNLKLVRPFMK*ELKEATFA 308
Score = 26.2 bits (56), Expect(2) = 4e-04
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = +1
Query: 448 PDGLNPGFYQFFFGM 462
PDG N GFY F+GM
Sbjct: 316 PDGFNLGFYHRFWGM 360
>TC233199
Length = 1105
Score = 43.1 bits (100), Expect = 8e-04
Identities = 25/99 (25%), Positives = 47/99 (47%), Gaps = 10/99 (10%)
Frame = +2
Query: 96 VKGPWR-------LTAFYGYPDHGRRRDSWELLRSLHSQS-DDPWCIIGDFNDHLSPSDK 147
++G W+ + Y D +R+ W + L S S + WCI+ DFN S++
Sbjct: 158 LEGTWKGDGEKITIVNIYSPCDVNLKRNLWGQINQLRSAS*NKLWCIVDDFNCVRRQSER 337
Query: 148 RGGPDRPHW--LIRGFQEAVSDCNLIDLPLNGYQFTWFK 184
G R ++ F + + D + D+P G+++TW++
Sbjct: 338 VGASQREQGGGIMTEFNDWIVDLEVEDMPSVGHKYTWYR 454
>CA936755
Length = 424
Score = 40.4 bits (93), Expect = 0.005
Identities = 27/93 (29%), Positives = 39/93 (41%), Gaps = 5/93 (5%)
Frame = -2
Query: 200 LCTAPWLELFPHASLQTLVAPMSDHTPLLLQLDPPPWRAPHNSFRFNNSWLIEPELAHLV 259
L + WL +P +S TL SDH P+++Q W FR + WL + LV
Sbjct: 291 LVSEQWLLSWPDSSQYTLPRDFSDHCPIIMQTKKVDWGP--KPFRVVDWWLHQKGYQRLV 118
Query: 260 KDNWSYYPSSN-----IVTKLSYCIEDMKYWSK 287
++ WS + KL +K WSK
Sbjct: 117 RETWSAEQQPGWGGILLKNKLRMLKLSIKQWSK 19
>TC206673
Length = 742
Score = 40.0 bits (92), Expect = 0.007
Identities = 23/68 (33%), Positives = 34/68 (49%), Gaps = 2/68 (2%)
Frame = +3
Query: 302 NQIDVMRNTSDASVDPRLI--ELQNSLANLILQEDVYWRQRSKIFWLKDGDKNSKFFHLT 359
N+++ + + SVD ELQ L + RQ+S+ WLK+GD NS +FH
Sbjct: 45 NEVEDLASNRSLSVDEIKAKKELQQQLWEASTAYESLLRQKSRDKWLKEGDNNSAYFHTV 224
Query: 360 ASSRRRKN 367
+ RR N
Sbjct: 225 INFRRHYN 248
>CA938853 similar to GP|1684847|gb|A pinin {Homo sapiens}, partial (2%)
Length = 422
Score = 39.3 bits (90), Expect = 0.012
Identities = 18/47 (38%), Positives = 24/47 (50%)
Frame = -3
Query: 104 AFYGYPDHGRRRDSWELLRSLHSQSDDPWCIIGDFNDHLSPSDKRGG 150
A Y + +RRD W L L + W IGDFN ++ +KRGG
Sbjct: 168 AVYASTAYVKRRDLWSELTDLQQKHLGSWPFIGDFNSNVGAQEKRGG 28
>BI321779
Length = 421
Score = 38.1 bits (87), Expect = 0.026
Identities = 17/47 (36%), Positives = 27/47 (57%)
Frame = +2
Query: 417 TENDNATLTKPFSIDEFKEAVFSMHSDKSPGPDGLNPGFYQFFFGMI 463
+ DNA L + F ++E K+ + S KSPGPDG + + F+ +I
Sbjct: 62 SNEDNAMLIQDFEVEEIKKVI*DCESSKSPGPDGYSLLLIKIFWEVI 202
>CO982668
Length = 870
Score = 35.4 bits (80), Expect = 0.17
Identities = 22/73 (30%), Positives = 30/73 (40%), Gaps = 1/73 (1%)
Frame = -2
Query: 193 EARIDRALCTAPWLELFPHASLQTLVAPMSDHTPLLLQLDPP-PWRAPHNSFRFNNSWLI 251
+ R+DR L W P + L D P + LD P +RF N+WL
Sbjct: 269 QVRLDRVLMNQEWRIESPIVKVTHLFHCK*DQ*PHHINLDVG*PMNRKRCPYRFQNAWLS 90
Query: 252 EPELAHLVKDNWS 264
E L++ NWS
Sbjct: 89 HDEFDSLIRHNWS 51
>TC232603 weakly similar to UP|Q39482 (Q39482) Legumin (Fragment), partial
(4%)
Length = 1123
Score = 35.0 bits (79), Expect = 0.22
Identities = 13/43 (30%), Positives = 25/43 (57%)
Frame = +1
Query: 112 GRRRDSWELLRSLHSQSDDPWCIIGDFNDHLSPSDKRGGPDRP 154
G++++ E++R + + WCI+GDFN S +++G P
Sbjct: 370 GKKQEWEEIIRIKNEMGGERWCIVGDFNAVCSEEERKGVETNP 498
>TC222762
Length = 879
Score = 34.7 bits (78), Expect = 0.29
Identities = 20/40 (50%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = +1
Query: 878 NLAPYSLAFSK-PNISLDGTSWIPILVTIQVILGGAFGVP 916
N+ + FSK NI G SW PILV +Q + G AFGVP
Sbjct: 274 NVNSFDR*FSKIVNIIPWGISWTPILVMVQALSGVAFGVP 393
>BM732560
Length = 422
Score = 33.9 bits (76), Expect = 0.50
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -2
Query: 335 VYWRQRSKIFWLKDGDKNSKFFHLTASSRRRKNTISKLRHPNGFW 379
+Y Q ++ W++DG++N++ + + + RRKN I R G W
Sbjct: 181 IYKLQNTRGQWIRDGERNNRHYKIKTITGRRKNRILMSRGH*GTW 47
>TC219606 similar to UP|O48722 (O48722) Heme oxygenase 2 precursor (HO2),
partial (66%)
Length = 948
Score = 32.3 bits (72), Expect = 1.4
Identities = 17/41 (41%), Positives = 25/41 (60%), Gaps = 1/41 (2%)
Frame = -2
Query: 953 FLLLLWLSCLSTICL-IRICPLGIMISSILFLTTKMLQQFF 992
+LLLLW+SC CL +R+ I++ + LTT +L FF
Sbjct: 170 WLLLLWISCNCRTCLGLRLGVGSIVVEWVSLLTTSLLPFFF 48
>TC230791 homologue to UP|Q7S6J2 (Q7S6J2) Predicted protein, partial (7%)
Length = 643
Score = 31.6 bits (70), Expect = 2.5
Identities = 29/95 (30%), Positives = 45/95 (46%), Gaps = 2/95 (2%)
Frame = -3
Query: 928 SVMVPASMFGTLVGYVHSLHLNPPLFLLLLWLSCLSTICLIRICPLGIMISSILFLTTKM 987
S+++ S F ++ HL FLL L+L C + + L C I S F +
Sbjct: 635 SIIIHRSFF-----FLRFCHLLLFFFLLFLFLLCFNML-LFGFCWGSIRFS---FSYNFL 483
Query: 988 LQQFFLSLLGTELWWILM--FGSTRSMVPTPLNQL 1020
FFL + + LWW+L+ FG +V + LN+L
Sbjct: 482 WLLFFLVVSSSCLWWLLVIMFGREVVIVESVLNEL 378
>BE329487 weakly similar to PIR|T14619|T146 reverse transcriptase - beet
retrotransposon (fragment), partial (15%)
Length = 376
Score = 31.2 bits (69), Expect = 3.2
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -2
Query: 434 KEAVFSMHSDKSPGPDGLNPGFYQFFF 460
K AV+ S KSPGPDGLN F + F+
Sbjct: 372 KSAVWQCGSVKSPGPDGLNFKFIKHFW 292
>BM891643 weakly similar to GP|9759426|dbj WD-40 repeat protein-like
{Arabidopsis thaliana}, partial (4%)
Length = 406
Score = 31.2 bits (69), Expect = 3.2
Identities = 24/67 (35%), Positives = 34/67 (49%), Gaps = 4/67 (5%)
Frame = -1
Query: 186 IGTSHAKEARIDRALCTAPWLELFPH---ASLQTLVAPM-SDHTPLLLQLDPPPWRAPHN 241
+ T +A ++R+D++ CT P + FP S QT A SDH P L PPP P +
Sbjct: 265 VPTDYADDSRLDQS-CTGP--DTFPRDC*GSHQTWPALE*SDHPPNSLPYTPPPPLLPFS 95
Query: 242 SFRFNNS 248
F + S
Sbjct: 94 KFNTHTS 74
>AW099854
Length = 411
Score = 30.8 bits (68), Expect = 4.2
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 1109 CNLTQSSVTCFLLRMIFPLFYLISLTG 1135
CN Q+ TCF+ +++ P+ L SLTG
Sbjct: 280 CNT*QAKSTCFVYKVLHPIIVLYSLTG 200
>BI497688 similar to GP|14517500|gb| K16N12.18/K16N12.18 {Arabidopsis
thaliana}, partial (17%)
Length = 423
Score = 30.4 bits (67), Expect = 5.5
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = +3
Query: 231 LDPPPWRAPHNSFRFNNSWLIEPELAHLVKDNWSYYPSSNIVTKLSYCI 279
+ P W + F +N+ WL+ L+ N S Y S + + +CI
Sbjct: 144 IGPQ*WTSARTKFYYNSGWLLLDSSGKLIGINISIYSPSRASSGVGFCI 290
>CD413613
Length = 623
Score = 30.4 bits (67), Expect = 5.5
Identities = 30/81 (37%), Positives = 36/81 (44%)
Frame = -2
Query: 1268 TTCPM*SLSLTARPLLTL*TPLISHKTSWETFYLLVRTYYPFMLALL*TLLGGKQMRLLI 1327
TT P S LT T T IS K E V + + +LL GK ++L I
Sbjct: 277 TTSPKSSWKLTVSS*WTPSTRAISKKMKLEIS*SSVFLNVQVLKTVQFSLLVGKPIKLFI 98
Query: 1328 P*QEHPYLTLAPMFSMMYLLI 1348
P*QEH L MF M L+I
Sbjct: 97 P*QEH----LDSMFVNMLLII 47
>BI968991
Length = 750
Score = 30.4 bits (67), Expect = 5.5
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = +1
Query: 332 QEDVYWRQRSKIFWLKDGDKNSKFFH 357
+ D+ W ++S W+KDGD + +FH
Sbjct: 13 KNDILWFRKS*AKWIKDGDHETGYFH 90
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.338 0.147 0.484
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,490,576
Number of Sequences: 63676
Number of extensions: 1234192
Number of successful extensions: 10073
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 9851
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10061
length of query: 1356
length of database: 12,639,632
effective HSP length: 109
effective length of query: 1247
effective length of database: 5,698,948
effective search space: 7106588156
effective search space used: 7106588156
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 65 (29.6 bits)
Medicago: description of AC146855.1