Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146817.2 + phase: 0 
         (145 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AW132833                                                               69  8e-13
BM954263                                                               68  1e-12
TC217405 similar to UP|Q9FLL9 (Q9FLL9) Gb|AAB80666.1, partial (20%)    54  3e-08
BU081020                                                               31  0.18
TC221250 weakly similar to GB|AAP37739.1|30725434|BT008380 At5g0...    31  0.24
BE331525                                                               30  0.31
TC233625 similar to UP|Q85DB9 (Q85DB9) NADH dehydrogenase subuni...    30  0.31
TC214473 similar to UP|Q39449 (Q39449) Specific tissue protein 1...    27  3.4
TC229283 homologue to UP|P93078 (P93078) Ap19 protein, complete        27  3.4
AW756419                                                               27  4.5
TC214785 similar to UP|Q6V8T3 (Q6V8T3) Chlorophyll a/b-binding p...    27  4.5
CD412998                                                               26  5.9
AW703778                                                               26  5.9
TC207242 UP|O22107 (O22107) Squalene synthase , complete               26  7.7
TC205877 similar to UP|Q9FJJ6 (Q9FJJ6) Arabidopsis thaliana geno...    26  7.7

>AW132833 
          Length = 385

 Score = 68.9 bits (167), Expect = 8e-13
 Identities = 32/61 (52%), Positives = 44/61 (71%)
 Frame = +2

Query: 44  NSLKVKEKDNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITSRPLQAAHDEYEEEAT 103
           N L++ E+  MK  CDEKR++Y+YM+ + +E G S  GKGE  + + LQ AHDEY+EEAT
Sbjct: 116 NELRIVEE--MKRQCDEKRDLYDYMVTRYREGGXSKGGKGETFSLQQLQTAHDEYDEEAT 289

Query: 104 L 104
           L
Sbjct: 290 L 292


>BM954263 
          Length = 454

 Score = 68.2 bits (165), Expect = 1e-12
 Identities = 32/51 (62%), Positives = 38/51 (73%)
 Frame = +3

Query: 47  KVKEKDNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITSRPLQAAHDE 97
           +++  ++MK  CDEKR VYEYMI Q KEKGKS SGKGE  T + LQAAH E
Sbjct: 300 ELRTVEDMKRQCDEKRNVYEYMIAQQKEKGKSKSGKGESFTLQQLQAAHAE 452


>TC217405 similar to UP|Q9FLL9 (Q9FLL9) Gb|AAB80666.1, partial (20%)
          Length = 1652

 Score = 53.5 bits (127), Expect = 3e-08
 Identities = 22/37 (59%), Positives = 29/37 (77%)
 Frame = +3

Query: 109 CPHIAKLGKGRRIKPLKHSNFNERNCRDKFRQDPCRF 145
           C HI +L +G RI+P  +S+FN RNCRDKFR++P RF
Sbjct: 306 CFHITELSRGGRIRPFIYSSFNPRNCRDKFREEPRRF 416


>BU081020 
          Length = 421

 Score = 31.2 bits (69), Expect = 0.18
 Identities = 24/90 (26%), Positives = 41/90 (44%), Gaps = 2/90 (2%)
 Frame = -2

Query: 18  KNIKKNGKFG--PIRVPRGVNNQIFEDSNSLKVKEKDNMKHHCDEKREVYEYMIIQPKEK 75
           KN +K  K G   +R     N+    + N+L VK+ D++ H        YE   ++    
Sbjct: 309 KNHRKGTKHGFDGMRYDSASNSSDHYEENNLVVKQTDSLLHG-------YEKCNLK---- 163

Query: 76  GKSNSGKGEHITSRPLQAAHDEYEEEATLA 105
              N  +GE++ S+PL   H   +  A+L+
Sbjct: 162 ---NVHRGEYVKSKPLNLVHKSIQSTASLS 82


>TC221250 weakly similar to GB|AAP37739.1|30725434|BT008380 At5g05190
           {Arabidopsis thaliana;} , partial (12%)
          Length = 1059

 Score = 30.8 bits (68), Expect = 0.24
 Identities = 20/87 (22%), Positives = 36/87 (40%)
 Frame = +3

Query: 37  NQIFEDSNSLKVKEKDNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITSRPLQAAHD 96
           N++ + SN   V   D++ +  D    V           GKS S +G+ +++ PL   HD
Sbjct: 315 NEVIDGSNPHSVSHADHISYSDDYGHSV-----------GKSYSSEGDPVSAAPLHPLHD 461

Query: 97  EYEEEATLAKQGCPHIAKLGKGRRIKP 123
              ++ T++      I +  K     P
Sbjct: 462 SAYDKQTVSSGTLEPITEKDKNASRSP 542


>BE331525 
          Length = 397

 Score = 30.4 bits (67), Expect = 0.31
 Identities = 10/16 (62%), Positives = 14/16 (87%)
 Frame = +3

Query: 54  MKHHCDEKREVYEYMI 69
           MK  CDEKR++Y+YM+
Sbjct: 336 MKRQCDEKRDLYDYMV 383


>TC233625 similar to UP|Q85DB9 (Q85DB9) NADH dehydrogenase subunit 4, partial
           (5%)
          Length = 890

 Score = 30.4 bits (67), Expect = 0.31
 Identities = 25/92 (27%), Positives = 41/92 (44%), Gaps = 6/92 (6%)
 Frame = +3

Query: 42  DSNSLKVKEK------DNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITSRPLQAAH 95
           DS+ L V+E       D+   H  E+ E Y+    +   + + +SG  + +     +A  
Sbjct: 153 DSSHLSVEELNEADTVDDDSSHLLEQNEGYKCQGKEESCEARESSGSEKEVEENNEEALQ 332

Query: 96  DEYEEEATLAKQGCPHIAKLGKGRRIKPLKHS 127
            E E    L+KQ    IAK  KGR+    ++S
Sbjct: 333 SEAELVKNLSKQRRCAIAKAHKGRKTVAARNS 428


>TC214473 similar to UP|Q39449 (Q39449) Specific tissue protein 1, partial
           (19%)
          Length = 946

 Score = 26.9 bits (58), Expect = 3.4
 Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 13/89 (14%)
 Frame = +3

Query: 31  VPRGVNNQI-FEDSNSLKVKEK--DNMKHHCDEKR----------EVYEYMIIQPKEKGK 77
           +P G+   + F+  N+ K +E+     K+HC+E            E    +      + K
Sbjct: 150 MPEGLQGLVSFQSENNPKTQEQLGKGSKNHCEESLVTNTQVNSDFEPIPSVTKYDDLEFK 329

Query: 78  SNSGKGEHITSRPLQAAHDEYEEEATLAK 106
           S+  K +   SRP    HD++E ++++ K
Sbjct: 330 SSIIKNDDFESRPSVTKHDDFELKSSVTK 416


>TC229283 homologue to UP|P93078 (P93078) Ap19 protein, complete
          Length = 988

 Score = 26.9 bits (58), Expect = 3.4
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = -3

Query: 114 KLGKGRRIKPLKHSNFNERNCRDK 137
           K+GK +RI P    NFN R C+ K
Sbjct: 815 KMGKRKRILPRNLFNFNLRTCKVK 744


>AW756419 
          Length = 172

 Score = 26.6 bits (57), Expect = 4.5
 Identities = 12/39 (30%), Positives = 24/39 (60%)
 Frame = -1

Query: 51  KDNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITSR 89
           + ++ H+  +K+ + +  I+  KEK K+ S + +HIT R
Sbjct: 130 EQSLSHYF*QKKFIRDQDILLQKEKNKNTSLQQKHITLR 14


>TC214785 similar to UP|Q6V8T3 (Q6V8T3) Chlorophyll a/b-binding protein type
           I (Fragment), partial (33%)
          Length = 733

 Score = 26.6 bits (57), Expect = 4.5
 Identities = 13/40 (32%), Positives = 23/40 (57%)
 Frame = -3

Query: 49  KEKDNMKHHCDEKREVYEYMIIQPKEKGKSNSGKGEHITS 88
           + ++ +KH+C  K+E+ ++     KEKGK    KG+   S
Sbjct: 422 RTENMLKHYCT-KQEI*DWFTG*KKEKGKRKEEKGDRFES 306


>CD412998 
          Length = 465

 Score = 26.2 bits (56), Expect = 5.9
 Identities = 10/29 (34%), Positives = 15/29 (51%)
 Frame = +1

Query: 114 KLGKGRRIKPLKHSNFNERNCRDKFRQDP 142
           +L +GRR  P  H +  ++ C DK    P
Sbjct: 292 ELARGRRAGPPPHKHIKQKLCSDKCHNGP 378


>AW703778 
          Length = 311

 Score = 26.2 bits (56), Expect = 5.9
 Identities = 11/42 (26%), Positives = 21/42 (49%)
 Frame = +3

Query: 68  MIIQPKEKGKSNSGKGEHITSRPLQAAHDEYEEEATLAKQGC 109
           ++I+P+++ +   G+  H   R L AA  +  E+     Q C
Sbjct: 9   LVIEPQQQAEGRGGQVAHGLGRRLTAAPQQAPEQLVPCAQNC 134


>TC207242 UP|O22107 (O22107) Squalene synthase , complete
          Length = 1671

 Score = 25.8 bits (55), Expect = 7.7
 Identities = 9/14 (64%), Positives = 13/14 (92%)
 Frame = +2

Query: 14  SKVDKNIKKNGKFG 27
           SK D++++KNGKFG
Sbjct: 164 SKFDRSVEKNGKFG 205


>TC205877 similar to UP|Q9FJJ6 (Q9FJJ6) Arabidopsis thaliana genomic DNA,
           chromosome 5, TAC clone:K19B1 (AT5g62460/K19B1_7),
           partial (60%)
          Length = 1321

 Score = 25.8 bits (55), Expect = 7.7
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +2

Query: 43  SNSLKVKEKDNMKHHCDEKREVYEYMIIQPKEKG 76
           S SLK   +  ++H CDEK ++   +  QP + G
Sbjct: 458 SGSLKYAHRKCVQHWCDEKGDITCEICHQPYQPG 559


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.315    0.133    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,474,104
Number of Sequences: 63676
Number of extensions: 76749
Number of successful extensions: 312
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 312
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 312
length of query: 145
length of database: 12,639,632
effective HSP length: 88
effective length of query: 57
effective length of database: 7,036,144
effective search space: 401060208
effective search space used: 401060208
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 54 (25.4 bits)


Medicago: description of AC146817.2