BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146806.1 - phase: 0
(790 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW457987 78 2e-14
BE555752 39 0.012
TC209674 weakly similar to UP|Q6GUF2 (Q6GUF2) NIM1-like protein ... 32 1.1
TC219242 similar to UP|SY61_ARATH (Q946Y7) Syntaxin 61 (AtSYP61)... 32 1.1
TC222792 similar to UP|Q71IS7 (Q71IS7) Magnesium transporter (Fr... 31 2.4
CO983808 31 2.4
CO980311 30 3.2
BU548932 30 4.1
TC235105 UP|Q9L9E5 (Q9L9E5) NovW, partial (5%) 30 5.4
TC219617 similar to UP|Q8LPJ3 (Q8LPJ3) Alpha-mannosidase, partia... 29 7.0
TC217196 similar to GB|CAD83013.1|45771904|ATH551263 mitochondri... 29 9.2
TC212146 29 9.2
>AW457987
Length = 245
Score = 77.8 bits (190), Expect = 2e-14
Identities = 44/76 (57%), Positives = 47/76 (60%), Gaps = 23/76 (30%)
Frame = +1
Query: 611 LFSVLDLQLQELNARFDEENTELLQ-----------------------CVSCLSPAKSFS 647
LFSVLDL L ELNARFDE+NT+LLQ CVSCLSP+ SF
Sbjct: 1 LFSVLDL*LHELNARFDEDNTKLLQCVSCLSPSSSFEAFDEDNTKLLQCVSCLSPSSSFE 180
Query: 648 AFDVNKLLRMAELYPN 663
AFDV KLLRM ELYPN
Sbjct: 181 AFDVKKLLRMVELYPN 228
>BE555752
Length = 418
Score = 38.5 bits (88), Expect = 0.012
Identities = 31/96 (32%), Positives = 43/96 (44%), Gaps = 3/96 (3%)
Frame = -2
Query: 56 SYHPDIQDEVRKAYLKIRRHQPPSNF---VYPWSDFRGTRRRFNKNWFNLYDWLEYSESK 112
+YH + QD + + + SNF VYP S RF WF + WLEYS +
Sbjct: 279 NYHANQQDGINS*--NVYKVWTISNFFMDVYPLSGQEDHPCRFQACWFRNFPWLEYS-LE 109
Query: 113 NLAFCLPCFLFKNVSNYGGDHFVGDGFGDWKNPRKL 148
FC C++F S+ F GF KN +K+
Sbjct: 108 GAFFCFQCYIFSKKSS----PFTSKGF---KN*KKV 22
>TC209674 weakly similar to UP|Q6GUF2 (Q6GUF2) NIM1-like protein 1, partial
(13%)
Length = 471
Score = 32.0 bits (71), Expect = 1.1
Identities = 21/53 (39%), Positives = 28/53 (52%)
Frame = +2
Query: 353 YDGASNMRGRFGGLKTLIQNENPSAHYVHCFAHQLQLALVACAKTHKPVSGFF 405
Y +S ++G G LK + NE+PSAH +LQL L A KT + FF
Sbjct: 26 YANSSALKGTNGNLKQVDLNESPSAH-----TRKLQLRLHALMKTVENGRRFF 169
>TC219242 similar to UP|SY61_ARATH (Q946Y7) Syntaxin 61 (AtSYP61) (Osmotic
stess-sensitive mutant 1), partial (85%)
Length = 986
Score = 32.0 bits (71), Expect = 1.1
Identities = 36/182 (19%), Positives = 74/182 (39%), Gaps = 2/182 (1%)
Frame = +3
Query: 356 ASNMRGRFGGLKTLIQNENPSAHYVHCFAHQLQLALVACAKTHKPVSGFFGKVNMLVNFI 415
AS+ R + G +K +++ S+ H + ++ L+ +H+ S +FI
Sbjct: 342 ASSARSQVGTMKKAMESGKGSSTTSHASVNGMRRELMRLPNSHQTDSSNQYAARDNDDFI 521
Query: 416 RASNKRQEMLRDKQLAQFAKLIEEGEIETGSGLNQDSSIARAGDT--RWGSHFRTLTSLM 473
++ + RQ +L +Q + +L E G GL + GS + T+ +
Sbjct: 522 QSESDRQTLLIKRQDEELDELSESVRRIGGVGLTIHDELTAQEKILDELGSEMDSTTNRL 701
Query: 474 TLYGAIVEVIVEVGNDPSFDKFGETVLLLDVLQSFDFIFMLYMMVEILGITNDLSLALQR 533
V ++++ + G+ +++L +L F F+F+L L L +
Sbjct: 702 DFVQKKVAMVMKKASAK-----GQIMMILGLLALFIFLFILVFFT*SLRWDQSLCCLYMK 866
Query: 534 RD 535
RD
Sbjct: 867 RD 872
>TC222792 similar to UP|Q71IS7 (Q71IS7) Magnesium transporter (Fragment),
partial (15%)
Length = 959
Score = 30.8 bits (68), Expect = 2.4
Identities = 22/77 (28%), Positives = 34/77 (43%), Gaps = 13/77 (16%)
Frame = -2
Query: 704 VETNKCKTFALVFKLLKL-----ALLLPVATASVERVFSAMKIVKSHLR--------NKM 750
++ KCKT FK LK+ LLP A + + S+ ++ SH R N +
Sbjct: 721 LKLQKCKTQVSKFKFLKVI*VNETKLLPTRAAKIPLMISSSIVISSHFRIFNCSQLSNAV 542
Query: 751 GDQWLNDRLVTFIERDV 767
+ DR T + RD+
Sbjct: 541 KANYSTDRWSTQLGRDL 491
>CO983808
Length = 691
Score = 30.8 bits (68), Expect = 2.4
Identities = 26/88 (29%), Positives = 45/88 (50%), Gaps = 3/88 (3%)
Frame = +3
Query: 283 FCVLIDESGDVSGREQMA--VVLRYVDGDGLVKERFLGITSVKETSAKSLKDALETMLSI 340
F +++D + D+S +EQM+ +V Y F I + + KSL + +I
Sbjct: 378 FSIILDCTHDISYQEQMSFVIVCTYFFNPNTSF*IFFRIFKNR*YN*KSLF*CVIMNETI 557
Query: 341 N-GLSFSSIRGQGYDGASNMRGRFGGLK 367
N GL S++R Q Y+ NM+G+ G++
Sbjct: 558 NIGLDISNLRVQDYNKGLNMKGKHQGMQ 641
>CO980311
Length = 807
Score = 30.4 bits (67), Expect = 3.2
Identities = 18/48 (37%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
Frame = +2
Query: 79 SNF---VYPWSDFRGTRRRFNKNWFNLYDWLEYSESKNLAFCLPCFLF 123
SNF VYP S RF WF + W YS FC C++F
Sbjct: 665 SNFFMDVYPLSGQXXHPCRFQACWFXNFPWXXYSLXXAF-FCFQCYIF 805
>BU548932
Length = 392
Score = 30.0 bits (66), Expect = 4.1
Identities = 16/47 (34%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +2
Query: 101 NLYDWLEYSESKNLAFCLPCFLFKNVSNYGGD-HFVGDGFGDWKNPR 146
+LY ++ L+FC+ C K V+ GG+ HF+ +G+ +WK R
Sbjct: 257 SLYKQSSTNKLSGLSFCVNCQ--KTVTKIGGN*HFLVNGYKEWKIER 391
>TC235105 UP|Q9L9E5 (Q9L9E5) NovW, partial (5%)
Length = 741
Score = 29.6 bits (65), Expect = 5.4
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2
Query: 544 LVNDTKKQLQEMRNEGWEELISRVVTICTKHEID 577
L+N K +L+E NE E+ + ++ C+ HE D
Sbjct: 284 LINK*KDELKEWHNETVEQTTTEIIRSCSSHESD 183
>TC219617 similar to UP|Q8LPJ3 (Q8LPJ3) Alpha-mannosidase, partial (33%)
Length = 1129
Score = 29.3 bits (64), Expect = 7.0
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = +2
Query: 752 DQWLNDRLVTFIERDVLFTISTDVILAHFQQMDD 785
D W+N R++TF D + + ++ + Q++DD
Sbjct: 686 DDWMNSRVLTFSGIDSSYALPDNIAIITLQELDD 787
>TC217196 similar to GB|CAD83013.1|45771904|ATH551263 mitochondrial
sulfhydryl oxidase Erv1p {Arabidopsis thaliana;} ,
partial (59%)
Length = 753
Score = 28.9 bits (63), Expect = 9.2
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +3
Query: 89 RGTRRRFNKNWFNLYDWLEYSESKNLAFCLPCFL 122
+ TRRR KNW+ Y LEY+ + N+ L FL
Sbjct: 261 QATRRRM*KNWYRCY--LEYTLAGNVEITLKKFL 356
>TC212146
Length = 674
Score = 28.9 bits (63), Expect = 9.2
Identities = 12/31 (38%), Positives = 14/31 (44%)
Frame = +2
Query: 131 GDHFVGDGFGDWKNPRKLANHATSNNSHVDC 161
G F G W+N R H + NSH DC
Sbjct: 404 GSDFDGAAHAQWRNLRYPCAHYENRNSHFDC 496
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.137 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,009,022
Number of Sequences: 63676
Number of extensions: 453831
Number of successful extensions: 2238
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 2222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2236
length of query: 790
length of database: 12,639,632
effective HSP length: 105
effective length of query: 685
effective length of database: 5,953,652
effective search space: 4078251620
effective search space used: 4078251620
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146806.1