BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146790.10 - phase: 0
(406 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC218241 similar to UP|ROL_HUMAN (P14866) Heterogeneous nuclear ... 54 2e-07
TC224116 50 2e-06
BU965508 47 1e-05
TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%) 47 2e-05
AW756593 45 6e-05
TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat... 42 5e-04
AW203333 42 7e-04
AW596916 41 0.001
TC220267 similar to UP|Q6K235 (Q6K235) F-box-like protein, parti... 33 0.30
AI495509 32 0.52
CD487321 32 0.68
NP520295 disease resistance-like protein GS5-3 [Glycine max] 31 0.88
TC225617 UP|Q84ZV8 (Q84ZV8) R 3 protein, complete 30 1.5
AI899939 similar to GP|29895912|gb| Glycosyltransferase involved... 29 3.4
TC225614 UP|Q84ZV7 (Q84ZV7) R 12 protein, partial (88%) 29 4.4
TC214775 UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37, partia... 28 7.5
TC222889 similar to UP|Q9FE83 (Q9FE83) Emb|CAB62116.1 (At5g67250... 28 7.5
TC221652 28 7.5
CO981263 28 7.5
TC232958 28 7.5
>TC218241 similar to UP|ROL_HUMAN (P14866) Heterogeneous nuclear
ribonucleoprotein L (hnRNP L) (P/OKcl.14), partial (4%)
Length = 1206
Score = 53.5 bits (127), Expect = 2e-07
Identities = 37/118 (31%), Positives = 61/118 (51%), Gaps = 8/118 (6%)
Frame = +2
Query: 32 INRVLSFLPTKDVVR---TCVLSK--RWMNRWT---SSITKLDLDDIDLSYNYNPLCSYC 83
+ RV FL T ++ + L+K +W ++ T S+ + + N LCS C
Sbjct: 452 VRRVFDFLETWGLINYHPSSSLTKPLKWDDKETKSDSASNSTESSSAPVKENTKRLCSGC 631
Query: 84 EEICTDYCYDYDKYKLTVCPRCHVCGNHKANLRDAEVYERRVEIDFESGMKIESGKKE 141
+ +CT C+ DKY LT+C RC+V GN++ + ++ R VEI E+ K + +KE
Sbjct: 632 KVVCTIACFACDKYDLTLCARCYVRGNYRVGVNSSDF--R*VEISEET--KTDWNEKE 793
>TC224116
Length = 520
Score = 50.1 bits (118), Expect = 2e-06
Identities = 27/60 (45%), Positives = 39/60 (65%)
Frame = +2
Query: 10 KSTQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKLDLDD 69
+S +M V+ D I S LP+S+I +L LP +D VRT +LS +W +W +SIT+L DD
Sbjct: 254 RSIRMGDVMGPDLI-SDLPQSIIESILVQLPIRDAVRTSILSSKWRYKW-ASITQLVFDD 427
>BU965508
Length = 447
Score = 47.4 bits (111), Expect = 1e-05
Identities = 26/59 (44%), Positives = 37/59 (62%)
Frame = +2
Query: 11 STQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKLDLDD 69
S +M + D I S LP+S+I +L LP +D VRT +LS +W +W +SIT+L DD
Sbjct: 266 SIRMDDAMGPDLI-SDLPQSIIESILVQLPIRDAVRTSILSSKWRYKW-ASITRLVFDD 436
>TC233295 similar to UP|Q7KVQ0 (Q7KVQ0) CG4038-PA, partial (6%)
Length = 557
Score = 46.6 bits (109), Expect = 2e-05
Identities = 28/67 (41%), Positives = 40/67 (58%)
Frame = +3
Query: 3 AGSSKVHKSTQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSI 62
A SS+ Q + ++D I S LP+ L+ ++LS LPTK V T +LSKRW W ++
Sbjct: 12 AESSEPPSHRQKPTTGDDDRI-SHLPDVLLLQILSLLPTKQAVITGILSKRWRPLW-PAV 185
Query: 63 TKLDLDD 69
+ LD DD
Sbjct: 186SVLDFDD 206
>AW756593
Length = 370
Score = 45.1 bits (105), Expect = 6e-05
Identities = 18/41 (43%), Positives = 29/41 (69%)
Frame = +3
Query: 25 SKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKL 65
S+LP++++ ++ F+ T++ V+TCVLSKRW N W T L
Sbjct: 72 SELPDNILLHMMDFMDTREAVQTCVLSKRWNNLWKRLSTLL 194
>TC222844 weakly similar to UP|Q9FFW4 (Q9FFW4) Similarity to heat shock
transcription factor HSF30, partial (6%)
Length = 746
Score = 42.0 bits (97), Expect = 5e-04
Identities = 20/41 (48%), Positives = 29/41 (69%)
Frame = +1
Query: 25 SKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKL 65
S LP+SL+ +++F+ TK V+TCVLSKRW N + +T L
Sbjct: 505 SALPDSLLFHMINFMDTKSAVQTCVLSKRW-NDLSKCLTNL 624
>AW203333
Length = 418
Score = 41.6 bits (96), Expect = 7e-04
Identities = 19/43 (44%), Positives = 30/43 (69%)
Frame = +1
Query: 18 IEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTS 60
+E D I S LP+++++ +LS +P +D VRT VLSK W W++
Sbjct: 76 MERDMI-STLPKTILHDILSRMPEEDAVRTSVLSKSWAEAWST 201
>AW596916
Length = 455
Score = 40.8 bits (94), Expect = 0.001
Identities = 24/65 (36%), Positives = 34/65 (51%), Gaps = 1/65 (1%)
Frame = +3
Query: 10 KSTQMASVIEEDTIN-SKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKLDLD 68
K+ Q EED S LP+ ++ ++ F+ K V+TCVLSKRW W +T L L
Sbjct: 225 KNMQSDKDREEDMDRLSDLPDFVLLHIMKFMSMKHAVQTCVLSKRWKELW-KRLTNLALH 401
Query: 69 DIDLS 73
D +
Sbjct: 402 SSDFA 416
>TC220267 similar to UP|Q6K235 (Q6K235) F-box-like protein, partial (7%)
Length = 586
Score = 32.7 bits (73), Expect = 0.30
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 27 LPESLINRVLSFLPTKDVVRTCVLSKRW 54
LP+ L+ R+L++LP + R +SKRW
Sbjct: 338 LPDDLLERILAYLPIASIFRAGCVSKRW 421
>AI495509
Length = 394
Score = 32.0 bits (71), Expect = 0.52
Identities = 17/50 (34%), Positives = 30/50 (60%)
Frame = +3
Query: 31 LINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKLDLDDIDLSYNYNPLC 80
L NR +S++P + V+T + NR+ S I +++ DD+ LS ++ LC
Sbjct: 36 LPNRYISYIPRTEAVKTNWH*VKLSNRYISFIPRIEADDVGLSNKFS-LC 182
>CD487321
Length = 638
Score = 31.6 bits (70), Expect = 0.68
Identities = 17/39 (43%), Positives = 21/39 (53%)
Frame = +2
Query: 27 LPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKL 65
L E L+ RVLS+LPT R + KRW + S KL
Sbjct: 326 LNEDLLERVLSWLPTSSFFRLTSVCKRWKSAAASVSFKL 442
>NP520295 disease resistance-like protein GS5-3 [Glycine max]
Length = 467
Score = 31.2 bits (69), Expect = 0.88
Identities = 22/48 (45%), Positives = 25/48 (51%)
Frame = +1
Query: 207 LTSHYLFNCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFK 254
LTS N TSLE L L H SS FP I ++K L LYG+ K
Sbjct: 259 LTSFPPLNLTSLETLALS-HCSSLEYFPEILGEMENIKHLFLYGLHIK 399
>TC225617 UP|Q84ZV8 (Q84ZV8) R 3 protein, complete
Length = 2697
Score = 30.4 bits (67), Expect = 1.5
Identities = 39/146 (26%), Positives = 56/146 (37%), Gaps = 21/146 (14%)
Frame = +1
Query: 207 LTSHYLFNCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFKIDTSS------- 259
LTS N TSLE LEL H SS FP I +++ L L+G+ K S
Sbjct: 2056 LTSFPPLNLTSLETLEL-SHCSSLEYFPEILGEMENIERLDLHGLPIKELPFSFQNLIGL 2232
Query: 260 -------------DCLTLNLPLLRKFDIKNCN-WSGGKDLIVEAPLLEIVSIEQDIEFYN 305
C +P L F NCN W + E + I+S E ++
Sbjct: 2233 QQLSMFGCGIVQLRCSLAMMPKLSAFKFVNCNRWQWVESEEAEEKVGSIISSEARFWTHS 2412
Query: 306 AASHDLHSQSIKFNALHLKQFTYSGY 331
++ + + F K+F + GY
Sbjct: 2413 FSAKNCNLCD-DFFLTGFKKFAHVGY 2487
>AI899939 similar to GP|29895912|gb| Glycosyltransferase involved in cell
wall biogenesis {Bacillus cereus ATCC 14579}, partial
(3%)
Length = 415
Score = 29.3 bits (64), Expect = 3.4
Identities = 24/77 (31%), Positives = 38/77 (49%), Gaps = 2/77 (2%)
Frame = +2
Query: 198 SFYQLPFSALTSHY--LFNCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFKI 255
SF+ L FS+LT H+ LF T+L + IKF SI++ +++ L F++
Sbjct: 20 SFFLLFFSSLTLHFLLLFTNTTLSPPSNSIVTKLLIKFGSIALPLI*IRIRLLLTCAFRL 199
Query: 256 DTSSDCLTLNLPLLRKF 272
+ C L P+L F
Sbjct: 200 LYLNHCSLLP*PMLILF 250
>TC225614 UP|Q84ZV7 (Q84ZV7) R 12 protein, partial (88%)
Length = 2367
Score = 28.9 bits (63), Expect = 4.4
Identities = 29/92 (31%), Positives = 37/92 (39%), Gaps = 20/92 (21%)
Frame = +1
Query: 207 LTSHYLFNCTSLEELELVLHVSSTIKFPSI-----SVHFGHLKLLKLYGIFFKID----- 256
LTS N TSLE LEL H SS FP I ++ HL+ L + + F
Sbjct: 2044 LTSFPPLNLTSLETLEL-SHCSSLEYFPEILGEMENITALHLERLPIKELPFSFQNLIGL 2220
Query: 257 ----------TSSDCLTLNLPLLRKFDIKNCN 278
C +P L +F I+NCN
Sbjct: 2221 REITLRRCRIVRLRCSLAMMPNLFRFQIRNCN 2316
>TC214775 UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37, partial (42%)
Length = 530
Score = 28.1 bits (61), Expect = 7.5
Identities = 13/41 (31%), Positives = 19/41 (45%)
Frame = -2
Query: 202 LPFSALTSHYLFNCTSLEELELVLHVSSTIKFPSISVHFGH 242
+PFS +TS F+CT+ L S + P +H H
Sbjct: 133 IPFSTITSRTFFSCTTFSPLHCGQRSLSLMVSPGDCIHCKH 11
>TC222889 similar to UP|Q9FE83 (Q9FE83) Emb|CAB62116.1 (At5g67250) (SKP1
interacting partner 2) (SKIP2), partial (26%)
Length = 411
Score = 28.1 bits (61), Expect = 7.5
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +3
Query: 214 NCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFKIDTSSDCLTLNLPLLRKFD 273
+C +L+EL L+ + +I+ + +L+ L L GI D +C+ L+K
Sbjct: 171 HCLNLQELVLIGVYPTFSSLAAIASNCRNLERLALCGIGTVGDAEIECIADKCVALKKLY 350
Query: 274 IKNC 277
IK C
Sbjct: 351 IKRC 362
>TC221652
Length = 638
Score = 28.1 bits (61), Expect = 7.5
Identities = 18/56 (32%), Positives = 30/56 (53%), Gaps = 4/56 (7%)
Frame = +3
Query: 165 FSLLINNKRDISLQNTWISSILNRRVKILRIHSSFYQLPFSALTSHYL----FNCT 216
+ +L+N+ +IS +T ++ NR + I Y + FS LTSH L +NC+
Sbjct: 414 YMVLVNSGSNISRFSTSVNKGKNRCLLNFNISFVRYFMEFSGLTSHKLLLLGYNCS 581
>CO981263
Length = 826
Score = 28.1 bits (61), Expect = 7.5
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = -1
Query: 53 RWMNRWTSSITKLDLDDIDLSYNYNPLC 80
+W+N++ S IT LDL DL + N C
Sbjct: 298 KWVNKYMSMITPLDLSIWDLLFRSNLSC 215
>TC232958
Length = 758
Score = 28.1 bits (61), Expect = 7.5
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = +1
Query: 10 KSTQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWM 55
+ T++ + E LP+ LI +L LP K +VR + K W+
Sbjct: 10 QETRLDLTVVERRKKMVLPQDLITEILLRLPVKSLVRFKSVCKSWL 147
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.324 0.138 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,327,133
Number of Sequences: 63676
Number of extensions: 331938
Number of successful extensions: 2384
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 2357
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2381
length of query: 406
length of database: 12,639,632
effective HSP length: 99
effective length of query: 307
effective length of database: 6,335,708
effective search space: 1945062356
effective search space used: 1945062356
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146790.10